LOCUS       NZ_CP139405            13848 bp    DNA     circular CON 08-DEC-2023
DEFINITION  Klebsiella aerogenes strain C242078 plasmid pC242078-3, complete
            sequence.
ACCESSION   NZ_CP139405
VERSION     NZ_CP139405.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38396735
            Assembly: GCF_034197875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Li,X.
  TITLE     Klebsiella aerogenes C242078 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13848)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/08/2023 12:54:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,993
            CDSs (total)                      :: 4,867
            Genes (coding)                    :: 4,801
            CDSs (with protein)               :: 4,801
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 35 of 66
            Pseudo Genes (internal stop)      :: 11 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139405.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13848
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="C242078"
                     /isolation_source="excrement"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pC242078-3"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2020-01-04"
                     /collected_by="Longjie Zhou"
     gene            60..920
                     /locus_tag="SM905_RS24360"
                     /old_locus_tag="SM905_24360"
     CDS             60..920
                     /locus_tag="SM905_RS24360"
                     /old_locus_tag="SM905_24360"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            1179..1655
                     /locus_tag="SM905_RS24365"
                     /old_locus_tag="SM905_24365"
     CDS             1179..1655
                     /locus_tag="SM905_RS24365"
                     /old_locus_tag="SM905_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="WP_013023839.1"
                     /translation="MKQITMSDMQQQSAAAVQSPRLRAHRNFHPELSDSVQRLAIAMEP
                     GTYVRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVLEMAAGTWHAVLS
                     LDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQVGDSTFAV"
                     /name="WbuC family cupin fold metalloprotein"
     gene            complement(1702..2577)
                     /locus_tag="SM905_RS24370"
                     /old_locus_tag="SM905_24370"
     CDS             complement(1702..2577)
                     /locus_tag="SM905_RS24370"
                     /old_locus_tag="SM905_24370"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase CTX-M-3"
                     /protein_id="WP_011091028.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQSG
                     GRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKKSD
                     LVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETF
                     RLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAA
                     SIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESRRDVLA
                     SAAKIVTDGL"
                     /name="extended-spectrum class A beta-lactamase CTX-M-3"
     gene            complement(2912..>3106)
                     /locus_tag="SM905_RS24375"
                     /old_locus_tag="SM905_24375"
                     /pseudo=""
     CDS             complement(2912..>3106)
                     /locus_tag="SM905_RS24375"
                     /old_locus_tag="SM905_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /name="IS1380 family transposase"
     gene            3163..3438
                     /locus_tag="SM905_RS24380"
                     /old_locus_tag="SM905_24380"
     CDS             3163..3438
                     /locus_tag="SM905_RS24380"
                     /old_locus_tag="SM905_24380"
                     /inference="COORDINATES: protein motif:HMM:NF024169.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="WP_001419186.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHLKNSGRSR"
                     /name="IS1-like element transposase"
     gene            3483..3587
                     /locus_tag="SM905_RS24385"
                     /old_locus_tag="SM905_24385"
     CDS             3483..3587
                     /locus_tag="SM905_RS24385"
                     /old_locus_tag="SM905_24385"
                     /inference="COORDINATES: protein motif:HMM:NF015365.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_321140168.1"
                     /translation="MDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFR"
                     /name="IS1 family transposase"
     gene            3544..3861
                     /locus_tag="SM905_RS24390"
                     /old_locus_tag="SM905_24390"
     CDS             3544..3861
                     /locus_tag="SM905_RS24390"
                     /old_locus_tag="SM905_24390"
                     /inference="COORDINATES: protein motif:HMM:NF015365.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_321140169.1"
                     /translation="MTGYGGRLWRTSFGERTLATLERLLSLLSAFEVVVWMTDGWPLYE
                     SRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY
                     Q"
                     /name="IS1 family transposase"
     gene            complement(<3877..4782)
                     /locus_tag="SM905_RS24395"
                     /old_locus_tag="SM905_24395"
                     /pseudo=""
     CDS             complement(<3877..4782)
                     /locus_tag="SM905_RS24395"
                     /old_locus_tag="SM905_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /name="IS1380-like element ISEcp1 family transposase"
     gene            5826..7124
                     /locus_tag="SM905_RS24400"
                     /old_locus_tag="SM905_24400"
     CDS             5826..7124
                     /locus_tag="SM905_RS24400"
                     /old_locus_tag="SM905_24400"
                     /inference="COORDINATES: protein motif:HMM:NF024700.3"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_097414638.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGIIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            7197..7565
                     /locus_tag="SM905_RS24405"
                     /old_locus_tag="SM905_24405"
     CDS             7197..7565
                     /locus_tag="SM905_RS24405"
                     /old_locus_tag="SM905_24405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(7802..7915)
                     /locus_tag="SM905_RS24410"
                     /old_locus_tag="SM905_24410"
     CDS             complement(7802..7915)
                     /locus_tag="SM905_RS24410"
                     /old_locus_tag="SM905_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            8331..8522
                     /locus_tag="SM905_RS24415"
                     /old_locus_tag="SM905_24415"
     CDS             8331..8522
                     /locus_tag="SM905_RS24415"
                     /old_locus_tag="SM905_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045265536.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8522..8944
                     /locus_tag="SM905_RS24420"
                     /old_locus_tag="SM905_24420"
     CDS             8522..8944
                     /locus_tag="SM905_RS24420"
                     /old_locus_tag="SM905_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9999..13004)
                     /locus_tag="SM905_RS24425"
                     /old_locus_tag="SM905_24425"
     CDS             complement(9999..13004)
                     /locus_tag="SM905_RS24425"
                     /old_locus_tag="SM905_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            13168..13725
                     /locus_tag="SM905_RS24430"
                     /old_locus_tag="SM905_24430"
     CDS             13168..13725
                     /locus_tag="SM905_RS24430"
                     /old_locus_tag="SM905_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     ARG             complement(1706..2577)
                     /name="blaCTX-M-3"
                     /gene="blaCTX-M-3"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-3"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             61..917
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(7803..8170)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.038"
                     /coverage="90.0"
                     /evalue="4.68e-157"
ORIGIN
        1 atccgctcat gagacaataa ccctggtaaa tgcttcaata atattgaaaa aggaagagta
       61 tgagtattca acattttcgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      121 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      181 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      241 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggtgcg gtattatccc
      301 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      361 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      421 gcagtgctgc cataaccatg agtgataaca ctgctgccaa cttacttctg acaacgatcg
      481 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      541 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      601 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      661 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      721 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      781 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      841 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      901 cactgattaa gcattggtaa ctgtcagacc aagtttactc atactgaaat aattgggggg
      961 tgattaccac cgtaaatttt tcccagacgg taaaaagaat tacagacttt tatcaactaa
     1021 aaaagtggcc tgaatatcag cactaccgtc aataccattt cagtaaatca ggatagggaa
     1081 aaattggcaa acccagttct tcacttgctc tgcgggttct gtcaccctga cgtcatacgg
     1141 taatcccccc caattcatta atcgacaagg tcatcctgat gaaacaaatt accatgagcg
     1201 atatgcagca acagagcgca gcggctgtgc aatcgcctcg gctccgcgcg caccgtaatt
     1261 tccatccaga attaagcgat tcggtccaac gtctggctat tgccatggaa cctgggacct
     1321 acgtgcgccc gcaccgacac cctcacacct tcgagctact gttgccatta aggggtcgtt
     1381 tcgtggtgct gaattttgac gatcggggta ccgtcaccca tcgggcgata ttgggggaaa
     1441 cctgtacggt gctggagatg gccgcaggaa cctggcatgc cgtgctgtcg ctggataccg
     1501 gtggcataat ttttgaagta aaacacggtg gctatcaacc cgtggctgcc gatgactatg
     1561 cgcactgggc tccagcggaa ggagaaccag gaaccacgga gcttatggcc tggtatgcgc
     1621 aagcgcaggt gggcgacagc acttttgccg tctaaggcga taaacaaaaa cggaatgagt
     1681 ttccccattc cgtttccgct attacaaacc gtcggtgacg attttagccg ccgacgctaa
     1741 tacatcgcga cggctttctg ccttaggttg aggctgggtg aagtaagtga ccagaatcag
     1801 cggcgcacga tcttttggcc agatcaccgc gatatcgttg gtggtgccat agtcaccgct
     1861 gccggtttta tcccccacaa cccaggaagc aggcagtcca gcctgaatgc tcgctgcacc
     1921 ggtggtattg cctttcatcc atgtcaccag ctgcgcccgt tggctgtcgc ccaatgcttt
     1981 acccagcgtc agattccgca gagtttgcgc cattgcccga ggtgaagtgg tatcacgcgg
     2041 atcgcccgga atggcggtgt ttaacgtcgg ctcggtacgg tcgagacgga acgtttcgtc
     2101 tcccagctgt cgggcgaacg cggtgacgct agccgggccg ccaacgtgag caatcagctt
     2161 attcatcgcc acgttatcgc tgtactgtag cgcggccgcg ctaagctcag ccagtgacat
     2221 cgtcccattg acgtgctttt ccgcaatcgg attatagtta acaaggtcag attttttgat
     2281 ctcaactcgc tgatttaaca gattcggttc gctttcactt ttcttcagca ccgcggccgc
     2341 ggccatcact ttactggtgc tgcacatcgc aaagcgctca tcagcacgat aaagtatttg
     2401 cgaattatct gctgtgttaa tcaatgccac acccagtctg cctcccgact gccgctctaa
     2461 ttcggcaagt ttttgctgta cgtccgccgt ttgcgcatac agcggcacac ttcctaacaa
     2521 cagcgtgacg gttgccgtcg ccatcagcgt gaactggcgc agtgattttt taaccatggg
     2581 attccttatt ctgaaagaga cgaaataaca acaacatgaa tagtcaatat tttacctgaa
     2641 gcgagccaca acgcgtccga ttttatgctt ccgaaaggca aatacggacg tcgccagaat
     2701 gaaacctaaa ttccacgtgt gttttttatt agcttcaaaa atcactattt cacgaagaat
     2761 ttagactgct tctcacacat tgtaacatta tttacaacca cctttcaatc atttttgata
     2821 aatcattgat ttcatctttg ctgcaatgat acttaataaa ctctgcaagt tatccacaga
     2881 gcaacactca attttattga tgatattctt attataccag acatttttca tacactccct
     2941 tgtacggata gttttccgac aacttcatga ttacatatct tgcggttttg attatttttg
     3001 ctgcaagaaa tacatacttc aaacgaaagg tctttatttg ctgtctgtat tctgaagagt
     3061 ccaaggaatc aaacttgaac aacaaaaata ggttatatga aagcatcggt aatggtgcca
     3121 acttactgat ttagtgtatg atggtgtttt tgaggtgctc ccgtggcttc catctccatc
     3181 agttgtccct cctgctcagc tactgaaggc gtggtgcgta acggtaaaag tactgccgga
     3241 catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt cacttacacc
     3301 gcttctcaac ccggtacaca ccagaaaatc attgatatgg ccatgaatgg tgttggatgc
     3361 cgggcaaccg cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac
     3421 tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgatgtgatt gtctgcgctg
     3481 aaatggacga acagtggggc tacgtcggtg ctaaatcacg tcagcgctgg ctgttttacg
     3541 cgtatgacag gatacggagg acggttgtgg cgcacgtctt tcggtgaacg cactctggcc
     3601 acactggagc gtcttctgag cctgctgtcg gcctttgagg tcgtggtatg gatgacggat
     3661 ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgttac
     3721 actcagcgca ttgagcgaca taacctgaat ctgagacaac atctggcaag gctgggacgg
     3781 aagtcactgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg
     3841 aacataaaac actatcagta agttggagtc attaccaaag tagtcgtatt cggaaccttc
     3901 taaaagtgat aattgtgctc tttctttttc tggtttcagt acgcgagata cgacaaatct
     3961 tctgtctttt tcccatttaa ctaattttgt atacagttct gtagtttctc taccttcttc
     4021 tcctttaacg aatacaattg atgaattcgt tgcttgtgag gtgagtgtag aataactttt
     4081 ggctttaatt aaatatttgc atccaagaga ttctatcgtt tcgataattt tttcatcaaa
     4141 gtagccacta tccattcgaa ataaaatttc taaatcgtct gatttgatgt tagcaacaat
     4201 ttctttgatc atttccgcag caccgtttgc agtgtaagta ttgccacttc ttacaaatcc
     4261 ggtaacatat gcttttaatt cgtcgcaaaa tgcaaattgg atattgtagc atcggtttcc
     4321 cagtttctta ggattatatc cttttgacgc accttcttga tgaccttcta cgttaattac
     4381 actactatca atatcaatcg taatggatgt caatttactt ttagtgagca gttttttaaa
     4441 gactttaaaa ttaatgtctc taaacatttg ggttgtcttg aagttgaagt ttcctagaaa
     4501 ccgtgacact gtttcaggtt cttttacgga aatatcaaac tcgttgacga ggggatcatt
     4561 ttgaagtagc tttagacgtt ctaacttatc aatgccaatg aagtgaccgc agagcatggt
     4621 ctttatatga ttcatcttga ttttatttgt tgagtcatta tcaaatacga ggtcattttc
     4681 aataaaatca aaaatcccat tgctttttgc attctcaagg agcagaaaaa gacctgcatt
     4741 tgatgttaga ttcttagctt tgaaatcaat tttattaatc ataattagaa ccccttttta
     4801 ctacttttct tactattatt ttaccatata tcgagtcata aaagctgata atttaacata
     4861 tttttgagca cttttctttc acccaatggg tgaaagctga atttcgaagg aatgcatatt
     4921 tatcaaggct ttgattatgc tttttgaagt actgacgtag aatctaggtc atatatactt
     4981 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5041 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgat
     5101 tcaggtcata agagacacag taaactgaca tagaaccatc ctctttaatc aaatgtgggg
     5161 cagcccgtta gggctgcagt cacaacttac ttcttctttt cacgattaat tggaattttg
     5221 aacatcccac cgtttggacg ggtgatccgt ttgcccttta ctgtgatata agggcgaaaa
     5281 atgtatgcct gagactcgtc tgtctgcatg gtgcatttcc tatttttcgc cacatgagtg
     5341 ttgatttttg ctatcaaaat tgtactgtag caaccaacaa atcttgtggc tggctttatc
     5401 aagctcttga ggtggtactc aagtgccgca tttgtgtatg gtttgttacg ggttagttct
     5461 ggtggtacag gctaacccac ttacttcaaa aaacgtcgtt tttctccttt catgcgtcat
     5521 ttcctagatg tttcttcact gtatcagctc cgctgagcat aaatgctaca aagagaaacc
     5581 atccccatcc tgaaaccgag tgtaaagcta gcactgctga gctgacaact gcggctaaag
     5641 gaaccatttg gtatagaaaa aatctcatag accccctgca tttagctttt tttaacaagt
     5701 gttccataac gtgctatcta ttaaacgcac ctgttgtttt agaacgcttg tatgctatcc
     5761 ttaagtcctg attttgtcaa tatttactca ttttgttgtt taaggatggc gtgaggggtg
     5821 ttcttatgat ggttaatttt agtctttcta acgcattttc ctttatggat gctcaaaata
     5881 tgagcatgtt ggcggtgtca tccgcgaaag acgacataaa cttggctaac gtggtttcag
     5941 tgagtgatgt tcggatactg aaatctgctc tgatatttgg ggctaacgct agcgggaagt
     6001 ctaatctcat gaaaacaatt tctttaatga aagatattgt tttgcattcc gtgcaagcta
     6061 ttgaaagtag cattacaaag aacgtaatac cgtttttgct tgatgaggat ggaccaaata
     6121 aaccatctga aatggaaatg acttttattt cagatggggt taaatataga tatggattgt
     6181 ctatttttca ggggaaaata aaagaagaat ggttgtacta cacaccaaaa tcaagagaaa
     6241 ccattctctt tgaaagagat ggtctttcta ttgaggtaaa taaggcggct tttagtgagt
     6301 caaacctttt tatcaaagaa gggattattc aaaaaacaag ggaggatgtt ccatttgttt
     6361 cggttgttgc tggttttaat ggtgagcatt caaaacgagt agttaattgg tttaacaata
     6421 tcgtattcct ttctggttct aatgaaggga tgtttacagc aattaccatg aagttaataa
     6481 atgagtctga ggattttaag gcttggctaa aaaaaatact tccagcattc cagattaatg
     6541 atatttacct cgatgaggtc gcacctgatt ttccatttgg aaatataaaa acagatgatg
     6601 ataatttaaa aaatttattt agttcaatac aaaattttgc aaaaaaccag aaggtttttt
     6661 ctcttagagt agtgaaaaaa acagctagcg gtgagatttc agtacctatt gattttgagt
     6721 ctgagggtac taaaaagata gtttatcttc ttggtccaat ctacaatgcc atgataaacg
     6781 gtaacttatt gttagttgat gagtttgatt ctaagtttca cacccttttg tctaagtttt
     6841 tgtttagaat attccatgcg caaagtgagg ggcttagcca aataatagct gctgttcagg
     6901 atgttaatct aatgaacact gattgtttta gacgtgacca gatttggttt gttgataaag
     6961 atataaaatc aggaagtagt catctctatt ctttggttga gtataaagag aagcagcgaa
     7021 ctttaaaatc atcgtatggg catgattatt tatctggtgc atttgatgct atcccattgt
     7081 ttgataattt agatgaattt gaaaaactta tgcctgaggg atgatttatg tcaaagagag
     7141 gccgcggtct tcgtagctct tcgaggaaag aggaaacaaa atcctcaaag agcattatga
     7201 aaactacaga tagatttatt tctgttagtt ttagatattt tcataacgtc gatgatattg
     7261 gtcagtctat tgattcttgg agggttgacg gcagtttgcc agatttaatt gaaaagcttg
     7321 tttatcttac agctaatggg ataacaaaag tcagctctga tggtattttt actaactaca
     7381 ctcgatttcc agattcagca gttaatgatt ttaaatgccc tgatgaaata tcagttgatg
     7441 agaactgggg tgttataaaa aatataggag gccagaaaag aagggttgct ggttttttaa
     7501 aggataatgt gttttatata gttttttttg ataaggatca tcggttttgg aagagcagaa
     7561 aataggctta gttacgattg tactgtatgt tgaggcaaga tcttcttgag atcactgtgt
     7621 tctcgtcgta atctctcgct ctgtaaacga aaaaaccgcc ttggcgggcg gttttttcga
     7681 aggttccaag agttggcgct cttagaacca aggtaactgg cttggaggag cgcagtaacc
     7741 aaaacttgtt ctttcagttt agccttaacc ggcgcataac ttcaagacta actcctctaa
     7801 atcagttacc agtggctgct gccagtggcg cttttgcatg tctttccggg ttggactcaa
     7861 gacgatagtt accggataag gcgcagcagt cggactgaac ggggggttcg tgcatacagt
     7921 ccagcttgga gcgaactgcc tacccggaac tgagtgtctg gcgtggaatg agataaacgc
     7981 ggccataaca gcggaatggc accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     8041 gagccgccta tcagaaatga taggtatctt tatagtcctg tcgggtttcg ccaccactga
     8101 tttgagcgtc cgtttctgtg atgcttgtca ggggggcgga gcctatggaa aaacggccgc
     8161 tgcgcggcct cctcttttcc tcctaccttg atcgctcggt tttttcgagc ttttataaga
     8221 atggtcttgc cgctcgccgc agccgaacga ccggagcgta gcgactgagt gagcgaggaa
     8281 gcggaaaaga gactggtttt acactgagca ctgacgctct gaggcttctt atgacgaaac
     8341 aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg ctcctgctgg
     8401 agaagcttaa cgagcttgct ctcgatgtcg aggccgacct ttgtgagaaa ctccacgatg
     8461 atgcggagca tcttttccgt acactgtcct cgagactgga tgaattacag gatggtaact
     8521 gatggccaga gtgtctatta gtgaagcggc acggcttgtt aaggttagtc gccccacaat
     8581 ttacaagatg attaacagtg ggaagttaag ttacacctct gttgtaaagc acgggaaggc
     8641 tattaaagtt attgatactt cagagcttat tagagtgttt ggttctcttg atggtgtcat
     8701 cgatactgta aaatatgatg taaaaagtga cgcagaatct acaggtgtta acagcgttgg
     8761 tttacatgat ttacaacata gaatcgcttt acttgaagct gaaaacgatg gtcttaaagg
     8821 ggctgttaag gctcgagatg agcatattga ttcccttcgt caggctatgc agcttctgga
     8881 gcacaagcac gaaccttctt caccacccca ttccccgtgg tggaaattct ggaaaaaatc
     8941 ctgaccccgt catgcttccg ttttcctctc cggccatccc ggggagttcc tgaaccttcc
     9001 caaaaatcgt cagatctctg aacgctcctc accactaaaa atacccacac cccacgcaaa
     9061 attaacattt tgcagatttg tatttaatat cagttggtta ctttcctgaa atttcgtttt
     9121 ttaaatttta tttatataga tccgactcta tgacgactcg tcatagagtc cccggcgagt
     9181 cgtcatagag tcggtaacga ctcaggacta taaaaacaat aagttaataa cgacccgact
     9241 ctagacacga ctccccgttt ggttgttttt tagactcggc gactctgggt acgactctag
     9301 tgaagattcg ccggtgaatc gtgtggtgag tcgtccggcg acttgccata acgtgattgc
     9361 cggccgtcga cgaacccgat ttctgaacgt gctgaaaacc ggttttccgg ctgcgccggc
     9421 aacgcttcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tgggaagaac
     9481 gccggaaaca ggacttaccc tggctgaatg gcgtgaattt tcgcgctatg cacttgcgtg
     9541 catactcatg catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt
     9601 tgttgcctgt tttaccggtt tcccgtcaga aacgccctga ggccgtttta gcggtgcgta
     9661 caattagggg gttatgttaa atccacacca gctacagacg taaaaaaagc cccgcagggc
     9721 tttatatcta tatatctgtt ctcatccaat cccatgtgtc ttgggtgagc catcctgcgt
     9781 attctttggt tacaccaatc acaagcaagg tgtcattatt gtccagatgt ctacgcaaac
     9841 gatccgacag ttgttgcgct gtttctccgg ttgatattag ccatgtggaa tctaaagggt
     9901 ggcaccaccc cggcgatttt tttaactcat catatagtgc attgtagttt tgacctgctt
     9961 gattcggggt ctgacgctca gtggaacgaa aactcacgtt aagcaacgtt ttctgcctct
    10021 gacgcctctt ttaatggtct cagatgtcct ttggtcacca gttctgccag cgtgaaggaa
    10081 taatggccga gcatattgat atgtccgtgg caaagcgggg agaggcgtgc gatatcttca
    10141 tcattcagtg tttcaccctg cgcccggaga tgatccaggg ctgcctgcat ataaatagtg
    10201 ttccataaca cgacggcgtt agtgaccagc cccagtgtgc ccagttgatc ttcctgaccg
    10261 tcggtatatc gttttcttat ctcacctttt tgaccgtgac agatggctct ggcaacggca
    10321 tggcgacttt ctccccgatt aagctgggtc agaatgcgcc ggcggtaatc ttcatcatca
    10381 atataattaa gcagatacag cgttttgttg atgcgcccca cttcaatgat tgcctgagtc
    10441 agtccggaag gacgttcact tttcagcaat gaacggacca gcactgaaac ctgtactttg
    10501 cccagcttca gggagccagc ggtccggatc atttcgtccc actgaaggac tatttttcgg
    10561 ggatctgatt gccctctggc aatatcattc agcacgccat agtcggcatc atggtccatt
    10621 cgccagaaaa ccgaagcacc ggcatcagcc aggcgtggag aaaactggta tcccagcagc
    10681 cagaaaaggc caaagacaag ttcgctggca cctgctgtat cggtcataat ttcggttgga
    10741 ttcagcccgg tctcctgttc cagaagacct tccagcacaa agatagagtc cctcagcgtc
    10801 cccggtataa cgatgccatg aaagccggaa tactgatcgg acacaaagtt gtaccaggtg
    10861 atccctctgt tattaccaaa gtatttgcgg ttcggtccgg cattgattgt tctgactggc
    10921 gtaacaaagc gcattccatc tgcagatgcc acttctcctc caccccatat ctgtgccagt
    10981 ggcagcgttg cctgaaaatc aaccagtctg gcattagcgc tggtgatagt ttcagcccgc
    11041 agatagttcg cttttgtcca gttcagccgg tgtcgggtca gtgcaggaac atttgatctg
    11101 atcagtggtt ccagaccgat attgcaggct tcagccatca gcacggcgct gatgctgacg
    11161 ggcagatcat caactctggc actggcttca ctagcatgga aaaactcatc agcaaatccg
    11221 gtatgggcgt taatttcgag cagcaactcc gttaaatcca ccggagggag tagatcactg
    11281 atcattttgc tcagtcgttt cagactgtcc ggctcatcaa gactggcgag gggagaaatt
    11341 gtcaaccggg gcttcgggcc agaaacatcg agttcgacag cctcattttc gcaaagacgt
    11401 gcagcaacct gtctgtaacg actatcaagc tgatgaccca gagattttat tgcttcctgc
    11461 gggtctgtcg ggtgccccaa agaacgataa accttaatcc ggtttgcctg ccagtcagca
    11521 ccctgtagta atcttgcacg aggatctccc caccggttac tgccggtaac gtagacatcc
    11581 ctccgcctca gactatcctg cagtttactg agaaagcaga gcgtgtatcc cctgcgggtg
    11641 atatgttttt ccttgttaat caccagccgt ttccatgacc gactgataat ttccgttggt
    11701 gcgtcgtcaa aaaactgccg ccgtgagctg aactcccggc tgaggtagtc acaggcattc
    11761 agagtggtaa ccccggcagg tgcggatgaa aatttaacgg tattcagcag atggggcagg
    11821 aaacgacgaa cgcgcccgta ctgctccacc atttcttcat gaaaattatc gtctgagggc
    11881 cgggcaattt cacggacaag cgtgatgatt tcagccagct tttgccttgg gatgtagctg
    11941 aacacctcag cacgaatcga ttcgtccggt gtttcttctt tcagcaggta cgaacatgcg
    12001 ctggcgagcg ccaatgcaga tttatccaga tccttcagcg agcggagccg ttttttctgc
    12061 ccaatctttc tggcgtcacg gatgataacg gccagcatgg cgtccagaac gtccaatgca
    12121 tcatccagcg ccagcgtttc ccatgcaagg acaaaggcaa ccagaaccgc catccttttc
    12181 tgcggtgaca tcctggcaat attgaacacc gaagtcatac cagcataacg tgcgagattt
    12241 ttcaggcgca cagccgggag tgtactcagg ttttcagcat gcaggccaaa atcgttcaga
    12301 gttttccagc gttcaattgc ttcattaaac gccggaccac tgatggtcac agggcccttt
    12361 ttcagtgatt ccagtaaaga caggcggctg caatcagttg gccccagcag catctccagc
    12421 tgtgaacgct gttcggctga cggtatcagt gccagtttgt tccacaggcg caacgtcgcc
    12481 ttttccctta cctctgaaat caaccgggtc agcgtagtgg ctccggggag aataatacga
    12541 tgttgcataa gccaccctgt cgccagatcg aaaagcaggc caggacgttc gttgcttatc
    12601 cagctccggg tatataaaag acgggtaagg cgaaatgtcc agggccaggc aaattcacga
    12661 tactgatagt gctgacgtat cagcgctgca tgctcacggc gggtattttc cctctgaccg
    12721 tattctgcaa gaacggtgat atcacgaatc ccgagctgtc tggcggtaaa atgccggacg
    12781 ccggaaggaa tatgattcat atcggtgagg aaggtgccca gaaaacggac acatccaatt
    12841 tgcagggcaa tgcccagacg gttgtgatca cctctgcttt ttccgataaa ttccttgtct
    12901 gcttcatcaa ggtgaaaata tcgtgccagc tgaagctcat ccggttcacc ggtgaatctg
    12961 ccatagcttt cagtctgctc agtggtcaga aagtcaacgg gcatatcggc ctccctgcct
    13021 gacgggcatt tagtaacatt tttccaaccg tacgaaatgt tataaattat cggacatcgt
    13081 aaaactgtta cattaatatg tctattaaat cgtaaatttg taataataga catgagttgt
    13141 ccgatattcg atttaaggta catttttatg cgactttttg gttacgctcg ggtctcaacc
    13201 agtcagcagt ctcttgatct tcaggtcaga gcactcaaag acgcaggtgt gaaagcaaac
    13261 cgtatattta ccgataaggc atccggcagt tcaacagacc gggaagggct ggatttgctg
    13321 aggatgaagg tggaggaagg tgatgtcatt ctggttaaga agctcgaccg tcttggccgc
    13381 gacactgccg atatgatcca actgataaag gaatttgacg ctcagggcgt ggcagtccgg
    13441 ttcattgatg acgggatcag taccgacggt gatatggggc aaatggtggt caccatcctg
    13501 tcggctgtgg cacaggctga acgccggagg atcctagaac gcacgaatga gggccgacag
    13561 gaagcaaagc tgaaaggaat caaatttggc cgcaggcgta ccgtggacag gaacgtcgtg
    13621 ctgacgcttc atcagaaggg cactggtgca acggaaattg ctcatcagct cagtattgcc
    13681 cgctccacgg tttataaaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
    13741 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
    13801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgt
//
