LOCUS       NZ_CP139386             2746 bp    DNA     circular CON 07-DEC-2023
DEFINITION  Klebsiella aerogenes strain C82067 plasmid pC82067_4_2k, complete
            sequence.
ACCESSION   NZ_CP139386
VERSION     NZ_CP139386.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38396342
            Assembly: GCF_034120075.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Li,X.
  TITLE     Klebsiella aerogenes C82067 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/07/2023 12:36:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,090
            Genes (coding)                    :: 5,031
            CDSs (with protein)               :: 5,031
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 34 of 59
            Pseudo Genes (internal stop)      :: 6 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139386.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="C82067"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pC82067_4_2k"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2020-08-05"
                     /collected_by="Longjie Zhou"
     gene            430..657
                     /locus_tag="SM909_RS26055"
                     /old_locus_tag="SM909_26055"
     CDS             430..657
                     /locus_tag="SM909_RS26055"
                     /old_locus_tag="SM909_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223229632.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHDGYT"
                     /name="hypothetical protein"
     gene            complement(705..893)
                     /locus_tag="SM909_RS26060"
                     /old_locus_tag="SM909_26060"
     CDS             complement(705..893)
                     /locus_tag="SM909_RS26060"
                     /old_locus_tag="SM909_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1222..1596
                     /locus_tag="SM909_RS26065"
                     /old_locus_tag="SM909_26065"
     CDS             1222..1596
                     /locus_tag="SM909_RS26065"
                     /old_locus_tag="SM909_26065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061335866.1"
                     /translation="MNGMSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSL
                     LGQGFSLPGGDKDTHSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKE
                     LNPDFTTYEIGRFLFKHLQG"
                     /name="hypothetical protein"
     gene            complement(1785..>1886)
                     /locus_tag="SM909_RS26070"
                     /old_locus_tag="SM909_26070"
                     /pseudo=""
     CDS             complement(1785..>1886)
                     /locus_tag="SM909_RS26070"
                     /old_locus_tag="SM909_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     Typing          complement(2208..2703)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.171"
                     /coverage="82.0"
                     /evalue="0.0"
ORIGIN
        1 tttaatacga tgcaggatag ggcaaacgcc gcaaaatgac gtcatccgac atcattccgc
       61 cgcgttcgtc cagtgaccta cagtcggctc tggggtgtcg gggctgtgcc ctgaccaggc
      121 gttttggagt atcgccaccg tggtggcgat actccaaaac atatcctgtc ccttccttgg
      181 catctctgaa gtttagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc
      241 gccccgacac ccgctaaaag cactgacgcg cctgcggctt gcccaacccc gcgccgtccg
      301 ggaaagaatt tcccgatcga cccgacgttt ctgtttacct gtgatgttaa cgtttggatc
      361 tttttcccgg gtttacctat tttcttctaa tgaagcgttc acagctgacg ttaatagatg
      421 ggtgctttca tgtttggttt ttcaccagga gaacttatga acctttgccc cgatgaacgt
      481 ctgctctttg tgcggatgat ttccgctatg cttcgccgtt cgggcggtga tgcgggtgct
      541 gttatgtttg aggcgtatcg ccatattgtg tccgatacca atcaagctcg tcgttcctac
      601 atgctcgacc tgctcgaaag cgtcaggcat gactatgtac acgatggata tacatagttg
      661 ccctggatac gcttttcgtc taagtgagcg ttcttttcgt ctctttattc gccgtcctgt
      721 ataccgaagc gtgcgctcag cttctgataa agcgcttctg cctgttcgtg cattcgctca
      781 cagtcggctg cctcgtcatc aagatcgagc acgtcgagtt tttcgagcag cagcagcgac
      841 tgcgcccgga taaattttgc catgttcagt gcggctgatt cctgttttgt catacggctt
      901 tttcccggtt ttatggtgat cgcagactga aatcttactt cattcacttg agtgcatagc
      961 catgcaggta cgcagaaatc atcagcgcgt ttctgagcgg ttttcagggg gtaagatgtg
     1021 ttcttttgca ggtgaggtcg tagaaacgcc ctgagagcct ctcaggcggt gcacgcaacg
     1081 gatgttatgt tcaatctggc ggtgcagcat agttatcatc gcacctacag accgcttgtt
     1141 gtattcagaa caatatttat ttgtataatt tgtattttgt tgtattattt cttcaaatta
     1201 gagcggtggg gtaataataa tatgaatggt atgtctgacg taagactaaa gaaatcaact
     1261 attgagagcc taaaagagta cgtgaatgac tttggcatga cggttgatga tgttgtcctg
     1321 aacttaattg aagagaataa ttcattaaaa tcgttattag gtcagggctt ttctttgcct
     1381 ggtggagata aggacacaca ttccttcaag gattacgtga ttcataagct tccaaaagga
     1441 aagatcttgg ttaccaaaga cgggcttaaa gttagcaaca tgaagaaggt attgatcgag
     1501 gcagggagcg ctgtagggat ggatgataaa gagctaaacc ctgactttac gacctatgaa
     1561 ataggccgtt ttctcttcaa acatcttcag ggataaaggt gtctaccagt tcgtggccgg
     1621 gattttaggt tcaattcagc tagttcccgg acagaaaaaa agccaccgtg taggtggctt
     1681 ttttattcct gctttaggca aagcattatt gatagcattt gcaagcaaac cgttagcgac
     1741 ttctgctgaa tgcttctggg cggattcaag ttcgaagtgc aacgctaagc cgcctgtctt
     1801 agctcagcaa agaccactga aggttgccgg aatccaagac atttccgtgg cctgctattc
     1861 agtgctctct ggtacttgcg gagctcccct gtgtgataag tcaggtgtgg aatttttacg
     1921 tataaggccc cgctaggcgg aaaccaatac tgaagccaaa tcactggtaa acagccggat
     1981 cttcttgtga tccttttact ctcgtcgtaa tctcatgtcc tgtaaacaaa aaaaccaccg
     2041 ctaccaacgg tggttcgttt gccggaatca agagctacca actctttctt ccgaaggtaa
     2101 ctggctttca cggagcgcag ataccaaaac tgtccttcca gtgtagccgc agtcaggccg
     2161 ccacttcaag aactctgtag aagcactcta ctaccctgct ccgtgaatcc tcttaccaat
     2221 ggctgctgcc aatggcgtta agtcgtgtct ttccgggttg gactcaagac gatagttacc
     2281 ggacaaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
     2341 aactgcctac ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg
     2401 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccaccagggg
     2461 gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     2521 ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggcttcgccg tggccttctt
     2581 gcttccctgt taagcttctt cctggaagat gccaggaaat ttccgccccg ccagtaagcc
     2641 ggtaccgctc gccgcagccg aacgaccgag cggagcgagt cagtgagcga ggaagcggaa
     2701 tatctccggg cttatctttg gcaccgtacg cgccatagct gcattt
//
