LOCUS       NZ_CP139385             6033 bp    DNA     circular CON 07-DEC-2023
DEFINITION  Klebsiella aerogenes strain C82067 plasmid pC82067_3_6k, complete
            sequence.
ACCESSION   NZ_CP139385
VERSION     NZ_CP139385.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38396342
            Assembly: GCF_034120075.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Li,X.
  TITLE     Klebsiella aerogenes C82067 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/07/2023 12:36:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,214
            CDSs (total)                      :: 5,090
            Genes (coding)                    :: 5,031
            CDSs (with protein)               :: 5,031
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 34 of 59
            Pseudo Genes (internal stop)      :: 6 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139385.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="C82067"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pC82067_3_6k"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2020-08-05"
                     /collected_by="Longjie Zhou"
     gene            join(5776..6033,1..291)
                     /locus_tag="SM909_RS26020"
                     /old_locus_tag="SM909_26020"
     CDS             join(5776..6033,1..291)
                     /locus_tag="SM909_RS26020"
                     /old_locus_tag="SM909_26020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059363236.1"
                     /translation="MAEKLTLLDMFKNGTATWSSVSSFVVVLFWCVGCWLAFIAIVNYK
                     NAADGKSGIAKPIIQTVIASLMVAISRFIPILSDTLNNKAAEFSPQSLLSDIPTTDTMG
                     LGLAFTSVLLFVQMLGTIAIFRGFLMIWEVTNKGAGSGLIGKSWTHIIGGVLAVNIQLT
                     ISTVASTFYPGVDLTFLGL"
                     /name="hypothetical protein"
     gene            complement(426..683)
                     /locus_tag="SM909_RS26025"
                     /old_locus_tag="SM909_26025"
     CDS             complement(426..683)
                     /locus_tag="SM909_RS26025"
                     /old_locus_tag="SM909_26025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_320903121.1"
                     /translation="MPNFEFKTINTFYDDDIARYLITEKITKKENINIHNLLKSVLISR
                     NIDFNKIDILDLSEDEIKEIKTSADEILADIDNYKKAYNL"
                     /name="hypothetical protein"
     gene            1469..3205
                     /locus_tag="SM909_RS26030"
                     /old_locus_tag="SM909_26030"
     CDS             1469..3205
                     /locus_tag="SM909_RS26030"
                     /old_locus_tag="SM909_26030"
                     /inference="COORDINATES: protein motif:HMM:NF013603.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_320903122.1"
                     /translation="MLYENNKAFKQIVKQLEAGQAYNFLPKKQYADLVNVAPTNAKTLN
                     RRKTENLKLSSIYQKTGQADRAHRVAFCCSYRYVLIDSENRAHTTSTVRCRDRHCSECQ
                     RIKSYVYQERLKRTAIEKIKSDFVEDEFLFLTFTVKNPLVWNMRKTMRAMSQAFGLLLK
                     RKIFLSVFRGGIRTFEVTRSFEELFKNFCHPHIHAIAQTKKDALILILAHIEAEEIKAL
                     KAIYREKRDKKNKEKLIKKIMLINEKYKTLSDSAKEEMIKVFFEEQWTDCLKECFKKND
                     LVWNAEDYESTKGRAIVDVKRVRAGGEKDAPILTGEGFKEHGEKVINYVLKYSLKEDDK
                     RIFTGDKWSQIFDQEIKGMRMIAPFGLWRSELSKEEKAEYNEKDYFDKFESVTDSNAYL
                     AKFDSEYKTETITAAEIKRVKRSQLCLSLDYLNRRYIDTVKELKDKLNIALQKTYLSKR
                     LEAIAINEIVIEINKYSEKVRANFEKLIQYGEYVKGERFYIKTYIQAESFYNNIDLEYQ
                     KNIHLFKYNDINNKDRNWKLELFEYLNEYRIEKIDSYQFAEIELIDVEEYAEIEVCEEA
                     PF"
                     /name="protein rep"
     gene            complement(3236..3613)
                     /locus_tag="SM909_RS26035"
                     /old_locus_tag="SM909_26035"
     CDS             complement(3236..3613)
                     /locus_tag="SM909_RS26035"
                     /old_locus_tag="SM909_26035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_320903119.1"
                     /translation="MSIKFKTSPQGIKITTYQPTNIKFEYENKLYRIEIYIEEPAENTK
                     VKGDKEKKYKPEEKEITFIKTKEIDLELLEAFDTRRKLTKVRHTTDYIPPEKNGVFKIR
                     YAAFSVEEEEKFLKYNNNAGY"
                     /name="hypothetical protein"
     gene            complement(3540..4607)
                     /gene="mobQ"
                     /locus_tag="SM909_RS26040"
                     /old_locus_tag="SM909_26040"
     CDS             complement(3540..4607)
                     /gene="mobQ"
                     /locus_tag="SM909_RS26040"
                     /old_locus_tag="SM909_26040"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_320903120.1"
                     /translation="MAIFHIDFKILKRSEGKSSLYLAAYNARMRLKDEKTGLVFNYEKK
                     KEDLMHSDILLPKNAPESFKNRSVLWNEVEKQKRKDSQLSRYFICALPRSLSLQENKKL
                     LEEYIQKNFINKGMCADYAIHNDAENNNPHAHVMLTMQNVNETGFLNKNREWNKKENID
                     IWRRSWSVSINKYLRQNKKSDYVTHLSFLKQREILIKKAKEELEKNNLDDAEKYLNVYN
                     DFKDKKPRKRKPRHTYLNRKNHKNKLKNYNQQLEDIKRKEKKKEDMKKENKLTSFFKNV
                     FNKLTNKQQKETEERKQREIEAMKQWKKNEEKRAELREKLKNENLNEEINYVDKIQNIP
                     TRDQNHNIPTHKYKI"
                     /name="mobQ"
     gene            4970..5377
                     /locus_tag="SM909_RS26045"
                     /old_locus_tag="SM909_26045"
     CDS             4970..5377
                     /locus_tag="SM909_RS26045"
                     /old_locus_tag="SM909_26045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059363234.1"
                     /translation="MIKTNKDFKNDIDCLANNIYNFYLDTLKENNYRIFAKDVNFKLDE
                     VDEYELNAFKKCFKVYLKTDAQFRKTKHIKSDCLSVSLPDFYNNYYTVNFIIYKDRYSE
                     FGKKYLDDIFNLFVKNIEYRVKNKEKINKGE"
                     /name="hypothetical protein"
     gene            5496..5762
                     /locus_tag="SM909_RS26050"
                     /old_locus_tag="SM909_26050"
     CDS             5496..5762
                     /locus_tag="SM909_RS26050"
                     /old_locus_tag="SM909_26050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059363235.1"
                     /translation="MTRLSVEQKKERLKRELAKIEAEEKKLKRAKELKDLKIINGEILR
                     YTKKDFDNFKKILGLLVLFETQNDEFRKAVFTSADREIQKRNK"
                     /name="hypothetical protein"
ORIGIN
        1 gaattttcgc cgcagagctt acttagtgat attccaacca ctgatacaat gggattgggg
       61 ctggcgttca caagcgtttt attgtttgtt caaatgttag gcaccatcgc catttttcgg
      121 ggctttttga tgatatggga agtaacgaat aagggagctg gttctggact cataggcaag
      181 agctggacgc acattatagg tggcgtttta gcggtaaaca tacaattgac catatcaaca
      241 gtagcatcca cgttctatcc tggtgtagat ctcacgtttt tgggtttatg agtggaaatc
      301 agtcaagagc agaagcgtag cgaagcgtag cgactcttga cggattggag cgaccccaac
      361 cctgagcaaa gggggaaagc tggccatcgg ctggctttcc tcctgttaag ctggttttaa
      421 tgtttttaaa ggttgtatgc ttttttataa ttatcaatgt cagctaaaat ctcatcagct
      481 gatgttttta tttcttttat ttcatcttct gataaatcta aaatatcaat tttattaaag
      541 tctatatttc tacttatcaa taccgatttt aataaattgt gtatatttat attttctttt
      601 tttgttattt tttcagttat taaatatctt gcaatgtcat catcgtagaa tgtgtttatt
      661 gttttaaatt caaagtttgg catctttctt cctcctgtgt tttttgctta tcatatcaga
      721 gagggcggca ggcgtctggg tgggtttgtt atcgtgtctg tcgtcctttc ctcatccctc
      781 ccgtatgtag agatacccga ttatctaaaa cggcttaaaa cgcgttacag agcgttacag
      841 agctattcct gagcatcttt tttcccgtgg cggtagctga tgggtttgac cttctccccc
      901 tcgggggttg ggggggttaa acccgttcag ggttcgccct tgaccttggt tttgggggtt
      961 gagcgaagcg acgccccccg cttgattata tagaaacaaa tcgcggcgtt tggcacaaaa
     1021 tagcgatttt ttactaagaa aaacctttat tttttaataa cttacaagtc tttttttcct
     1081 tcaatttttg aaattttttt ttcttaaaat ttggttaaaa aataagcaat ttttaagatg
     1141 ggacggcata caccaaaacg gcaaaaaatc gggttgattt tgggacggca tacaccaaaa
     1201 cggcaaaaaa tcgggttgat tttgggacgg gttacttttt gaaagtttcc gatagggggc
     1261 agacgtggga caacatgccc aaaaacggca aaaaatggtg gttgaaatgg gacggtacga
     1321 tcaaaaacgg caaaaaatgg gggttgaaat gggacggcgt cacacggtgg gggatttttg
     1381 agcgttttaa taagtgattg atgtgattga caaaaacaaa aaacccctta gagtctgagt
     1441 tgcgaagtca agaaactaag gggtttaaat gctttatgaa aataataaag cgtttaagca
     1501 gattgtcaag caactggaag ctggtcaagc atataatttt ttacctaaaa aacaatatgc
     1561 tgatttggtt aatgtagcac cgacaaacgc taaaacgcta aatcgtcgaa agacagaaaa
     1621 tttaaaatta tcttctattt atcagaagac agggcaagct gatagagcgc atcgcgttgc
     1681 gttctgttgc tcatatcgtt atgttttaat tgatagcgaa aacagagcac acacaacaag
     1741 cactgtcagg tgtagagacc gacactgcag cgaatgtcag cgcataaaat cttacgtata
     1801 tcaagagcga ttgaaacgca ccgcgataga aaaaatcaaa tctgattttg tcgaagatga
     1861 atttttattt ttaacgttca cggtaaaaaa tccgcttgtc tggaatatgc gcaaaacaat
     1921 gcgagccatg tcgcaagcgt tcggtttatt acttaaaaga aaaatctttt tgtcggtttt
     1981 tcgcggtgga ataaggacgt ttgaagtaac tcgtagtttt gaagaattat ttaaaaactt
     2041 ctgtcatcct cacattcacg ccatagcaca gactaaaaaa gacgctttga ttttgatttt
     2101 agcgcatatt gaagcagaag aaataaaagc cttaaaagca atttatagag aaaaaagaga
     2161 caagaaaaat aaagaaaaat tgattaaaaa aataatgtta attaatgaaa aatataagac
     2221 gttaagcgat agcgcaaaag aagaaatgat taaagttttc ttcgaggaac aatggacaga
     2281 ttgcctgaaa gaatgcttta aaaagaatga tcttgtatgg aatgcggaag actacgaaag
     2341 cacgaaaggg cgggcgattg tggacgttaa acgcgtaagg gctggcggtg agaaagacgc
     2401 ccctattttg acgggtgaag gttttaaaga gcacggcgaa aaagtgatca attatgtgct
     2461 caagtacagt ttgaaagaag atgataagcg tatttttaca ggtgataaat ggagtcaaat
     2521 atttgaccaa gaaataaaag ggatgagaat gatcgccccc tttggtttat ggcgttctga
     2581 attgtcaaaa gaagaaaaag cagaatataa tgaaaaagat tactttgata aatttgaaag
     2641 cgtgacagat agcaacgctt atcttgctaa atttgactct gaatataaaa cagaaacaat
     2701 aacagcagca gaaattaaac gcgttaaacg ttcgcagctt tgcttatccc ttgattattt
     2761 aaatagaaga tatattgaca cggttaaaga attaaaagac aagttaaata tagctttaca
     2821 aaaaacatat ttatcaaaaa gactcgaagc aattgccata aatgagattg ttattgaaat
     2881 aaataaatat agcgaaaaag tgcgggcgaa ttttgaaaag ttgatccagt acggtgagta
     2941 cgtaaaaggt gagcgttttt atattaaaac atatattcaa gcagaaagtt tttacaacaa
     3001 tatagatctt gaatatcaaa aaaatattca tttatttaaa tataatgaca ttaataataa
     3061 agatcggaat tggaaacttg aattgtttga atatttaaat gaatacagaa ttgaaaaaat
     3121 tgacagctat caattcgcag agattgagct tattgacgtt gaagaatacg cagagattga
     3181 agtatgtgaa gaagctccat tttaaccaaa tataaagccc cataaggggc tttttttaat
     3241 atccagcatt gttgttatat tttaaaaatt tctcttcttc ttcaacgcta aaagcagcat
     3301 aacgaatttt aaagactcca tttttttcag gcggtatata atcggttgta tgtcttactt
     3361 ttgtaagttt ccgccttgta tcaaaagcct ctaatagttc aagatctatt tcttttgttt
     3421 ttataaatgt gatctctttt tcttctggtt tatatttttt ctctttatct ccctttactt
     3481 ttgtattttc ggcaggttct tcaatataaa tctcaattct ataaagtttg ttttcatatt
     3541 caaattttat atttgtgggt tggtatgttg tgattttgat cccttgtggg gatgttttga
     3601 attttatcga cataattaat ttcctcattt aaattttcat tttttaattt ctctcgtaat
     3661 tccgcgcgtt tttcttcgtt cttcttccat tgtttcattg cttcaatctc tctttgtttt
     3721 ctttcctccg tttctttttg ttgtttatta gttaatttat taaatacatt tttaaaaaat
     3781 gaagtcaatt tattttcttt tttcatatct tcttttttct tttcttttct ttttatatct
     3841 tcaagttgtt ggttgtaatt tttcaattta tttttatgat ttttacgatt taaatatgtg
     3901 tgacgcggtt ttcttttgcg tggtttttta tctttaaaat cattgtagac attaagatat
     3961 ttttcagcat catcaagatt atttttttct aattcttctt ttgctttttt tattaaaatc
     4021 tcgcgttgtt ttaaaaatga tagatgtgtg acatagtcac tttttttatt ttgacgtaaa
     4081 tatttattaa ttgaaactga ccacgaacga cgccatatgt ctatattttc ttttttattc
     4141 cattctctat ttttatttaa aaatcctgtt tcatttacgt tctgcatcgt taacataacg
     4201 tgagcatgag gattattatt ttcagcgtca ttgtgtatag cgtaatcagc gcacatccct
     4261 ttatttataa aatttttttg aatatattct tcaagcaatt ttttattttc ttgtaatgat
     4321 aaagagcgcg gtaaagcaca gatgaaatag cgcgacaatt gcgaatcttt tcgcttttgt
     4381 ttctcgactt cattccataa tactgatcta tttttaaagc tttcaggcgc attttttggc
     4441 agtaaaatat cactatgcat caggtcttct tttttctttt catagttaaa aacaagacct
     4501 gtcttttcgt cttttaatcg catacgagcg ttgtaagctg cgagatataa agaactcttt
     4561 ccctcgctgc gttttagtat tttaaaatca atgtgaaata tagccattgg tcacccctcc
     4621 cctgtcgtta gccttgcgtc attttgggca ctttttaacg ttgcgtagct tcgttaaaac
     4681 tgtgcttatt gccccctttt aggggcaatc tagggcggta gcccaaggcc cccgccaggc
     4741 ccacacgttg cgaagcaatg tataggtggg catttgtagt ttttactcgt ttcactcgca
     4801 aaaacaaaaa tgagataaga ctaacttaat aaacatttat aaaaacttaa taactcaaaa
     4861 aataaacaag tcaaaccatt gacaatataa aaaaatatat taattgatga gtatacccaa
     4921 taaataagag gattgcggtg aagatttata aaaataaaag gagaatcaca tgataaaaac
     4981 gaataaagat tttaaaaatg atatagattg tttagctaat aatatttata acttttattt
     5041 agacacattg aaagaaaata attatagaat atttgccaag gacgttaatt ttaaattaga
     5101 tgaagttgat gaatatgaat taaacgcttt taaaaaatgc tttaaggttt atttaaaaac
     5161 agatgcgcaa tttaggaaaa caaaacatat taaaagcgat tgtttaagtg tttccttgcc
     5221 agatttttat aataactatt acaccgttaa ttttattatt tataaagatc gttatagtga
     5281 gtttggaaaa aaatatttag acgatatttt taatctgttt gttaaaaata tagagtacag
     5341 agttaaaaat aaagagaaaa taaacaaagg agaataaagt gattataact gtaatattga
     5401 atgttgttgt tatttcttct ttgtctgttt tgtatatcaa gaccaaaaaa aaagaaaaat
     5461 cgaaattaaa aaatataaaa taaaggtggt gaattatgac gagattaagc gtagagcaaa
     5521 aaaaagaaag attgaaaaga gaacttgcta aaattgaagc agaagaaaaa aaacttaaaa
     5581 gagcaaaaga attaaaagac ttgaaaatta ttaatggtga aatattaaga tatacaaaaa
     5641 aagattttga taattttaaa aaaattttag ggctattggt tttatttgaa actcaaaacg
     5701 atgaatttag aaaagctgtt tttacttctg ctgatcgtga aatacaaaaa agaaacaaat
     5761 aagcgggggt gacatatggc tgagaaatta actttattag atatgtttaa aaatggtacg
     5821 gctacttggt catctgtttc atcttttgtc gttgttttgt tttggtgtgt ggggtgttgg
     5881 ctggcattta tagcaattgt taattataaa aacgccgcag atggaaaaag cggaatcgca
     5941 aagccaatta ttcaaactgt gatcgcttct ctcatggtcg ctatttctcg atttattccg
     6001 attctttcag acactttaaa caataaagcg gct
//
