LOCUS       NZ_CP139370            10060 bp    DNA     circular CON 07-DEC-2023
DEFINITION  Klebsiella aerogenes strain C21992 plasmid pC21992_2_10k, complete
            sequence.
ACCESSION   NZ_CP139370
VERSION     NZ_CP139370.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38378067
            Assembly: GCF_034119905.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Klebsiella aerogenes C21992 Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2023) Laboratory Medicine Center, Department of
            Clinical Laboratory, Zhejiang Provincial People's Hospital
            (Affiliated People's Hospital, Hangzhou Medical College), Shangtang
            road, Hangzhou 310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/07/2023 12:57:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,034
            Genes (coding)                    :: 4,955
            CDSs (with protein)               :: 4,955
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 49 of 79
            Pseudo Genes (internal stop)      :: 12 of 79
            Pseudo Genes (multiple problems)  :: 18 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139370.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="C21992"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pC21992_2_10k"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2019-01-02"
                     /collected_by="Longjie Zhou"
     gene            complement(92..241)
                     /locus_tag="SM789_RS24680"
                     /old_locus_tag="SM789_24680"
     CDS             complement(92..241)
                     /locus_tag="SM789_RS24680"
                     /old_locus_tag="SM789_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(326..583)
                     /locus_tag="SM789_RS24685"
                     /old_locus_tag="SM789_24685"
     CDS             complement(326..583)
                     /locus_tag="SM789_RS24685"
                     /old_locus_tag="SM789_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(593..2278)
                     /locus_tag="SM789_RS24690"
                     /old_locus_tag="SM789_24690"
     CDS             complement(593..2278)
                     /locus_tag="SM789_RS24690"
                     /old_locus_tag="SM789_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2605..2850
                     /locus_tag="SM789_RS24695"
                     /old_locus_tag="SM789_24695"
     CDS             2605..2850
                     /locus_tag="SM789_RS24695"
                     /old_locus_tag="SM789_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3124..3495
                     /gene="tolA"
                     /locus_tag="SM789_RS24700"
                     /old_locus_tag="SM789_24700"
     CDS             3124..3495
                     /gene="tolA"
                     /locus_tag="SM789_RS24700"
                     /old_locus_tag="SM789_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3492..3857
                     /locus_tag="SM789_RS24705"
                     /old_locus_tag="SM789_24705"
     CDS             3492..3857
                     /locus_tag="SM789_RS24705"
                     /old_locus_tag="SM789_24705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            4032..4310
                     /locus_tag="SM789_RS24710"
                     /old_locus_tag="SM789_24710"
     CDS             4032..4310
                     /locus_tag="SM789_RS24710"
                     /old_locus_tag="SM789_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4298..4612
                     /locus_tag="SM789_RS24715"
                     /old_locus_tag="SM789_24715"
     CDS             4298..4612
                     /locus_tag="SM789_RS24715"
                     /old_locus_tag="SM789_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4776..5204
                     /locus_tag="SM789_RS24720"
                     /old_locus_tag="SM789_24720"
     CDS             4776..5204
                     /locus_tag="SM789_RS24720"
                     /old_locus_tag="SM789_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            5230..5409
                     /locus_tag="SM789_RS24725"
                     /old_locus_tag="SM789_24725"
     CDS             5230..5409
                     /locus_tag="SM789_RS24725"
                     /old_locus_tag="SM789_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5436..5966)
                     /locus_tag="SM789_RS24730"
                     /old_locus_tag="SM789_24730"
     CDS             complement(5436..5966)
                     /locus_tag="SM789_RS24730"
                     /old_locus_tag="SM789_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5973..6704)
                     /gene="mobC"
                     /locus_tag="SM789_RS24735"
                     /old_locus_tag="SM789_24735"
     CDS             complement(5973..6704)
                     /gene="mobC"
                     /locus_tag="SM789_RS24735"
                     /old_locus_tag="SM789_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6704..8668)
                     /locus_tag="SM789_RS24740"
                     /old_locus_tag="SM789_24740"
     CDS             complement(6704..8668)
                     /locus_tag="SM789_RS24740"
                     /old_locus_tag="SM789_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7278
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9471..10049
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5977..6704)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atcaaaccac ctccccaggt ggttttttcg tttacagggc aaaagattac gcgcagaaaa
       61 aaaggatctc aagaagatcc tttgatcttt tctactgaac cgcgatcccc gtcagttcag
      121 aggaggacga tggtgccacc gttccgccct gaacatcccg gatataattt gcctgacatg
      181 cggccagaag aaagccagca acaatcaata taaaaagaaa aatagtcttt acctttttca
      241 tatactttcc ctgtatttaa agactccggc attcatccct gaacaccgga agtcagtatt
      301 tcaaatcatc cttgataatt ttttgttacc agttaccgtc ccgataatca aaagctatat
      361 aacaattatc cacatcaggt tttatttgat ttttaaaaaa tggctgtagt aaaggaaccc
      421 attctttctt caaatcaaac tcaccattat taatattatc ctttaaaggc attcccaagc
      481 tttcaatgac agaaccatcg tcaccaaagt ctttagaata ctcttcccct ttaaactctt
      541 cagttttctt atcaaaccat gtcaaacgta atttaagccc catatttacc tctcaaagat
      601 attttttaat gttccgtttt ggatctggcc ctttaacctg cttacccgtt ttgggatcaa
      661 atgcgcctag gtgttcgcca tcactggcac gatacccttc aagctcaccg tgctgggaat
      721 cccattcata aattttacgt tttttatcgc cataccatcg ggcccgctta ccaccaccac
      781 cttgtttagg tgttttagga tcacctcgct ttaactctcc aagtcccttt atgtcttctg
      841 ttttaggtac tggatgataa tcatggccgt agtctttagt ccccttgcga ggcttgttct
      901 tttcctcatt taacttattt tctgcatcct tcgctttttt ctccttttgt ttccggctct
      961 ccatcgcccc ggtaagtgca gccttatctc tggataagga ttcattcatt gcgtttaaag
     1021 atgcctgagc cgcttttaat ttctcattct gattctgaat atccttatca agatagcgaa
     1081 cctgatcctg ccacccggag ccatattcat gaggcgggtt ataagtcaca aaatcatctt
     1141 tatttgcctc tgcctctttt acctttttat cggcttcctg aacagcagcc cttgcaccgg
     1201 ggagcgcctt ctctgtcgat gcaatgcggc tgtttaaggt cgcaatattt ttatcttcgg
     1261 catccagctc agctttcgct ttatcatagt ctcttttcag cccctcttcc ggatgagcgg
     1321 cgtcccatgc ctgctggcga cgcttttctt cctcctgacg ctgttttacc tgtgccgggg
     1381 taagaacatc tgtcaccgac acataaaccg gcttctgtcc ggtctctttc gggaaacgaa
     1441 taacggcctc acgggagtta ccaccggctg taaaaccagc cggacgtgaa tcaccttttt
     1501 cagcaacaat gccttttggc ggggctttcg ctgtcggaac acctttcggt acgctcacct
     1561 gcagagacgg gagacccgga atcgacacac tgaatacccc cggacgtttt gtcggtttcg
     1621 catccaccac agggacactc attggccgac ctgcgacaac cgcaatatgc tgccgctcat
     1681 ccttcaccac atccacaacc cgttgtctga cacgaaccgt cgcctggtcc agtggtaaag
     1741 tacttgccgg agtctccgtc accgcatcgg ccggtaatga cgtcataatt tttgacatca
     1801 ttttcggatc atcttttgca atctcagaag gcagcacacc gtagatcgca atcccccaca
     1861 gaccaaactt aaacggcccc ttcagggcag ccagcacatc agcaacggcg gccgacaacg
     1921 catcgccgga aactgataaa gccagtcctt cagcccccgg agtagccagg gcaggcagac
     1981 cgaaggccat cgcagtggcc gaagactgtc cgccaccaga gctaccgcta tgattgccgc
     2041 tgttaccacc attaccaccg ggtttactcc cgccgttacc aaactccccc ggattatagt
     2101 tatgaatatc tccattcggc gtatgcgtcg taccccagcc cggcccgctg tttggatccg
     2161 taccaccacc tgatgaaggg ccacctttac cagacgtacc gttcacattc ccactggcct
     2221 gaccaccatt atgtcccaga cctgaattac ccggacctcg accgtctcca ccactcatac
     2281 cgttttcctc tttgacattt aaaacaataa attaaagcca acatactgta catatacaca
     2341 gtataattat taccttactc ctcccgtttt caggtgtcaa tatatgagat tagttctcat
     2401 cttcaggcac aaaaaagcgc ccggagccac ctgtaagcag atcgcgtctt ttcccgaaaa
     2461 tttctcatgg caacaaattt tattatttaa gacattcatc acacttacat ttcagatggt
     2521 atagacacat ctgaaagcta actggcatga ggagaggacc tgcggttaaa ctgttcggga
     2581 aacctgagac gcccggaggc cactatgacg cacctgaaac tgactcccgc tgaagaaaag
     2641 cgcctggcgg acgcctttgt cgctcaggca ctggccatgg gcctgaaagg gaacaaaacg
     2701 ctgccaaagg cggtaaaaca acaggtgcgg gaaagggtgc ttcgtgccat tcaggacgaa
     2761 cgtgaagcca ggcgggcgca ggaaatgcgt cgggagaaat acgaggctac ggaaaccacc
     2821 ttcacctggc agccacagcg ccgccggtaa ggggtattta ccataacgtc gcattgcccg
     2881 caccgctcag acggtctgag ggcgtttccg gcggctacgg gataaaacag gcaacaaacc
     2941 acccggaaaa ccgccggaaa cgcgcccggt aaattgtaac ccgctgcatg cctatgcacg
     3001 caactgcatg gcgggccgtt tggtgtaaaa acgatacgat aagccgtgtt tatgtaaatg
     3061 aacgtggccg taaagagtga caaatataca tgtaacattt tttactgaca ggtcatcaac
     3121 ccgatgcgtt taataccctt tatcctgctg gcattgtgca gccccttact cctgacggcc
     3181 tgtaagcatt ccaccaccca gccgccgaaa atttatactc tggataagtc atctaaaatc
     3241 agtagctacg ctgcggccat caagaccgct gttgaaatgc agatgtttga accagaggcc
     3301 tatgcgggaa aaatgtgcac cgtgcggata tccatacaac gtgatggcac tctgaacagc
     3361 gccattgctg aaggaggaga tccgaaactc tgtaaagcag caatatcagc cgttacccac
     3421 gccaaaattc cgccggcacc ggatgaaaat acctggcaaa tattcaaaaa cgcccctctg
     3481 gatttcagac catgaaagct tttactctga cagtgctggc ggccatgttc ctcactccgg
     3541 tacatgcgat accactaaaa cccgtaacca cactgcagcc aacctacccg gtacaggccg
     3601 cagcaaaacg catcgaaggt caggtgaccg ccacgtttga tattctggac gatggacgag
     3661 tggacaacgt gtcgcttacc ggctgtcacc ttttttttcg ggaaacgctg acggcaatga
     3721 ggcactggct atatcccgca gggcacccgg caatgcgaca ggttataaat tttgaattca
     3781 gtctgaaaaa tgacggctta caggatccgc gaccagtcac cacccaaaag ctcaatgatg
     3841 atggttgccg ccagtaacag gtcctttaac ccgcaggctg caatagtcct ggatgatgag
     3901 tgagcctgta gcagccctga ttagtggaag cgaagctgaa aaatgggcag caaaagccgt
     3961 atttctggca aatagcgccg ccacggatta aagtagtcat gtgtaataca tatgactaca
     4021 aaggggcttt tatgtcatca gcaacatcca tcaggctcga tgaagaactt aaggaccggc
     4081 tgaaaacgct ggccgatgac cggcaccgct cagcgcacgc gctaatgctg gaggcaatca
     4141 ccgaatacat cgaccgggaa gagaagcgca gccagtacct gcgggacgga caggccgcat
     4201 ggcagcacta tcaggaaacg ggtctgcacc ttactgcgga agaagcggaa gcctggataa
     4261 gtacctgggg aactgagaac gagcaggatg ctccgccatg ccacaggtaa cgatttccgc
     4321 gctggcacag cgcgatttac aacgccttca ggactttctc aaaaccaaaa atcggctggc
     4381 agccagaaaa ggaggtgagg tgatcgtccg ggctatccag caactgaaaa cattgccaga
     4441 cattggccgc ccggtgccgt ttctgccgct ggaatataag gaactggtga tcgggtttgg
     4501 cgacagtggc tatgtgatgc tctaccgcca cgaccgggaa atggaccaga ttgtgatcgt
     4561 caccgtcagg catcagaaag aatccgggta tccgggggca gacagcctct aataaccgat
     4621 gacgggggcg caattcattg cgtggccgtc gccggtttga caagcgcagc gcgtcagaaa
     4681 accacatcct gaacggaaaa acgggtaaaa ttcagcgata catttaccgt gagaaattac
     4741 acacctttca cacccgataa aatcaggggt atacagtggc gaaaacattg ctcaaccttt
     4801 cacaggcagc acaagccgcg ggcattacca gaagaacgct atacaatcat gtaaaacagg
     4861 gaaaggttac cgtgtcacgt gacgggaaaa acaaccctgt cgtggatgtg tcagaactga
     4921 tccgtgtata cggaaatgtg aatatccctg agaaacaaat tcccggcatt tcacaccgtg
     4981 aaaatacaca aaaaaatttc ccacaggaac agcttctggc aatgcaaaag gaactggccg
     5041 atctccggca ggctgtcacc ctgatgctgg aagataaaac atcccgggaa gaagaacgcc
     5101 gccagcacga tgatgagcgc cggaaactcc aggccgaagt ggacagattg acaacagaac
     5161 tgacacagaa gaaaaaacgc ttctggtccg gctggtttag ctgagaactt accgaaggga
     5221 atggagaaca tgacgaagca ggaaaaaact gccctcaata tggctaaatt cattaaggat
     5281 cagtcgctgc tgttgctgga aaagctgaac gagctggaca tggatacccc ggccgacatg
     5341 tgcgaacggc ttcacgagga tgcggaaaca ctctggcagg tgatgcggga taagctcggg
     5401 gaagaataac agcaccggcc atgccggtgc tggcgttatt ttttgccgaa cggccaccac
     5461 cggcgggcgg gtttgtcctc cggcagttca ggcaccggca cgttaaaata gcggctgtta
     5521 tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc gtgccgctcg
     5581 ctgaccacct caatcatcgc gttaatctgc tccggtgagc acttcccggc atcaaccacc
     5641 tgcacaccgt tctcgatggc gaaccgctcc acctttcgat agaggtaatc acacagcttc
     5701 attcctgtgg gtttgtcccg ttcccattgc ggattttcac cggtgaaaaa gtacgtggct
     5761 ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat cgccagatgc
     5821 tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag caccaccatg
     5881 tcttccagat gctcgctggt tggcataatg cagcgctcgc cgtctttgtc ttccacgtcc
     5941 tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa tttaaagcac
     6001 gcgaacagcc tctcccggtc cagctccacc gttttgcggg cctcgccggt ctgtggattc
     6061 acgatttcat ccaccgccag aatcgcccgc tgtacccgga caagcgtcgg ccggtcaggg
     6121 cagatgtacc agatttcatc gatgccgttc gcccggcggt tgaacagata cccggacacc
     6181 acttcctgat accgcctgcg gctcttgacg gtgcgctcta cctcaaatgc caccgttctg
     6241 ccgtccagct ccgccagcgc gtccggcact ttcatccctt tcagggccat acggtgcagg
     6301 taccgccagc cggtggcccc ccggccctcc atggagaggc gcagggactg cacggccagc
     6361 cggtgcggca gctgcgccgc cgacaccctt gacggctgaa acggaataac gtccgtcagc
     6421 ggctcgtcct cgtcgaacga gaacgccacc ccgtgcggcg tcagccccca cacgctgacc
     6481 tgacggccgt caaccgggta tttcgcccgg accagcagcc ctttcgccac cagactgtcc
     6541 aggaagcggt aaaccggctg taccgccgca atccccagcc gtcgcgcaac cagctccgcc
     6601 gtggaacaca gctcttcccg cagccagcgc agggtcagcc gtgttttctc cgcgttccgc
     6661 gcctgccgct tcgcgtggct gacgttgtac tgctccagtg tcatcagtaa agccccgcaa
     6721 aatcatcatc cgcttttggt ccgccgccgt cggccggagg caggtactcc gccagcgggt
     6781 ccgttttcgc cagcaccggg aaggttttca gcgaaatttc ccggcgctca acccgcagcg
     6841 ggctgctgaa cgccagccgc gccacgccca gcccggtcat caccgcgaac ccgtcaggca
     6901 ggtgttgcag ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg aagctgtcgc
     6961 cgctgatgtg ctccacgttc cccgcctccc ggctggcccg gcggcgctcc acgtccaccc
     7021 ggatctcccc ggtctggccc gccgcccact gcgcggactc ctggctggcc gcgcgataga
     7081 accagcggat cggcgtgtta tccagtaccg tggttttcac aaaatcagcg ggcagatcct
     7141 gcccgcactg accgaagtcg cccagcgact ggtgcgcgag cagcaggttg cagtcccggc
     7201 tgcgcagcgt ccccagcgcg ttcagcacgt acttcgacag cagatattta atctcatcga
     7261 gcatgatgct cgcgtgcggc cacctccgga actcatcccg cgcaatgatg atttgcgcgc
     7321 agcgggcaaa cagcatcttc tgcacccgga tcaccgcctc atcgtccatc gagccgatga
     7381 cgtacaggca gccgccgccg ttgattatcc ccgccacatc gataccgctg tccgtctgca
     7441 ggacgctcag gtcggccacg ttctccagct tggtgatcag ccccttcacg tcctttttca
     7501 gggcctccgg caacgcgtac gccgccgcca gcacctgctg aatgtttgcc ccctgcggga
     7561 actgctccgc aatcagcttc gccgctttct gctcgctgat gcggtagtgg tccgccacgt
     7621 cgccggtctc actcaggtta aacccggcaa ccagcagatt cttcagggcg tactggtcga
     7681 gatcgcggaa cagattcagc tgcgccggtt tccccggccg cagatccagc agggtgaagt
     7741 ccgggcagtg cgcccggaag acgtgcggcg cccacgcgtc ccctttgggg tcgaagtaca
     7801 ccacagcgtc cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc atcgcgctct
     7861 tgccgctccc gggcaggccg aggatctgaa tattggtctt gcgccaggta tcacgaggga
     7921 ccagcaccgg ctggccctgc tcatccttac ccagaaacac cgcctgctgt ttccgggcct
     7981 gctccgtgga aaaatcggcc agcgcgttaa ccggtaccgg ctccccgaaa tgcaggtggc
     8041 ggacgtctgt catttctccg gtccgctggc tgctgcgccg gctctcctga ttcagtttcg
     8101 cgttcaccgc ctcgtcagcg gccgcaaaat gaccatcaag cagcaggaac gccagcagcc
     8161 gcgccagaag gacgccggcg acaagccctc cggcacacca ggctatcccg aacaggttag
     8221 ccggctgcag caggtaataa tctgcgttcg catagaggtc cgggcgcagg acggtttctc
     8281 cccagctctc gataaacacg gcgacaacca gcccccccag aagggccaga ccggcagcgc
     8341 cggtcagcca gtacgcgtag ttccagacat gtcgccgtgc cctgaccatc cgcccgggtg
     8401 aatccagcga ggccagcacc gaaaatggca cagtccggtt attatcgcgc ttatagcgat
     8461 cggcatactc ccgccgcgcc ttatggctca gccccacaaa acgcattcgg ccggcccaga
     8521 gttcagccag accgaaaaac aggccgataa ccagaaaaaa accggccaga ctgtaatact
     8581 cccggtacag ccagctgacc cacgggtaaa tctgccgggc cagctccggg atatgccact
     8641 gccaggcaag cgaatccgta ttaaacatgc aatctctctc cttcagacga cgggtttacc
     8701 ggcgtgatgc gcagcccccg ctgcacaagc cgcggacatg ccccaaggca tggcggcggc
     8761 ggtcacgtcc gtggtgattg cgttcagctt tcaccgcgga ggccagcctc tttccgcccc
     8821 atgcggaaaa ggcgcagccc cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa
     8881 ggcggcaccc cagtgcggtc ttttccggtg ttccggcccc cgtcagagtt cgccatagcg
     8941 agcggctgac cacccgcaaa atcaggagca gctttgctgc ttcgtcaggt gcgtccagca
     9001 ccgtggattt ttgtgggtgg tcaggcgtga gcctgacggg ggccgggtga atttctggcg
     9061 tggccggcgt ccagccggac agagccagaa agaggggaaa agaccgtgcg tcggtggcgt
     9121 ccagccagcg taagcaaggt cttttcccct gaagtcagcc acgccccagc ggggatgact
     9181 tgcatttctc cgcaaggaga aaaaagcaac agagataggt taacgtaaaa tgttcagcga
     9241 aaaaccgaca tggttcacct atcctgataa ttgatcgtca gggaatagaa agacttaatc
     9301 aggggacaat atcccacatc agcgcgtccg gtgaccgtct tgcaggtgcc ggggaaattg
     9361 cgcctcctcg ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
     9421 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
     9481 cggcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
     9541 aaacagacgc tttttcggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
     9601 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggtaggt cgctccctct
     9661 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gtccgccgtt ctcccttgcg
     9721 ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg taggtcgttc
     9781 gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc gccttatccg
     9841 gtaactgttc acttgagtcc aacccgggaa agcacgcaaa acgccactgg cagcagccat
     9901 tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt
     9961 gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag
    10021 ttaccacggt taagcagttc cccaactgac ttaaccttcg
//
