LOCUS       NZ_CP139363             4362 bp    DNA     circular CON 07-DEC-2023
DEFINITION  Escherichia coli strain EC147566 plasmid pEC147566-3, complete
            sequence.
ACCESSION   NZ_CP139363
VERSION     NZ_CP139363.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38397467
            Assembly: GCF_034119705.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Li,W., Chen,J. and Li,X.
  TITLE     Complete Genome Sequence of Escherichia coli strain EC147566
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Li,W., Chen,J. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2023) College of Biological and Environmental
            Engineering, Binzhou University, Huanghe Road, Binzhou 256600, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. v 0.13-r308; Canu v. v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43.46x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/07/2023 13:01:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,833
            Genes (coding)                    :: 4,546
            CDSs (with protein)               :: 4,546
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 287
            CDSs (without protein)            :: 287
            Pseudo Genes (ambiguous residues) :: 0 of 287
            Pseudo Genes (frameshifted)       :: 104 of 287
            Pseudo Genes (incomplete)         :: 175 of 287
            Pseudo Genes (internal stop)      :: 46 of 287
            Pseudo Genes (multiple problems)  :: 36 of 287
            Pseudo Genes (short protein)      :: 4 of 287
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139363.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC147566"
                     /isolation_source="ascites"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC147566-3"
                     /geo_loc_name="China: Guangzhou"
                     /lat_lon="23.19 N 113.33 E"
                     /collection_date="2022-04"
                     /collected_by="Jing Chen"
     gene            30..947
                     /gene="mobV"
                     /locus_tag="SM146_RS24745"
                     /old_locus_tag="SM146_24745"
     CDS             30..947
                     /gene="mobV"
                     /locus_tag="SM146_RS24745"
                     /old_locus_tag="SM146_24745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_309487782.1"
                     /translation="MASALQHCYRERETPNANAERTPENYCSVSKSTDQAMGRVRELLP
                     EKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDE
                     ATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVKKLGLERGIEGSRATHQT
                     VQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTI
                     AMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQMTEILMMAQRFKQQNQEK
                     EKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(1017..1247)
                     /locus_tag="SM146_RS24750"
                     /old_locus_tag="SM146_24750"
     CDS             complement(1017..1247)
                     /locus_tag="SM146_RS24750"
                     /old_locus_tag="SM146_24750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(1234..1716)
                     /locus_tag="SM146_RS24755"
                     /old_locus_tag="SM146_24755"
     CDS             complement(1234..1716)
                     /locus_tag="SM146_RS24755"
                     /old_locus_tag="SM146_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     gene            2423..3391
                     /gene="mobV"
                     /locus_tag="SM146_RS24760"
                     /old_locus_tag="SM146_24760"
     CDS             2423..3391
                     /gene="mobV"
                     /locus_tag="SM146_RS24760"
                     /old_locus_tag="SM146_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(3461..3691)
                     /locus_tag="SM146_RS24765"
                     /old_locus_tag="SM146_24765"
     CDS             complement(3461..3691)
                     /locus_tag="SM146_RS24765"
                     /old_locus_tag="SM146_24765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(3678..4160)
                     /locus_tag="SM146_RS24770"
                     /old_locus_tag="SM146_24770"
     CDS             complement(3678..4160)
                     /locus_tag="SM146_RS24770"
                     /old_locus_tag="SM146_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          4..944
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.541"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2415..3388
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gctgcaaaaa gctgacggga atgggaagcg tggccagcgc gttgcagcac tgctaccgag
       61 agcgtgagac gccgaacgct aacgctgaac ggacaccaga aaactactgc tctgtgtcca
      121 aatcaacgga tcaggcaatg gggcgtgtgc gggagctgct gccggaaaaa cgccgtaaag
      181 atgcggtact ggccgttgaa tacgttatga cagcaagccc tgagtggtgg aaagaggcca
      241 caccacggca gcaggcggag tttttcgccc gctctgagca atggctggag aagaaatacg
      301 ggaaggatcg tgttgtggcg gcggtggttc accgggacga ggccaccccg cacctgtctg
      361 cgttcgtggt gccattgacg caggatggac ggctgtccgc gaaagagttt attggcgggc
      421 ggtcaaagat gcgtgaagat cagagcacct acgctgaatc agtaaaaaaa ctgggtcttg
      481 agcgcggcat cgagggaagc cgcgccacgc atcagacagt tcagcactac tacgaatcga
      541 taaaccgtgg cacccgtagc caggtatcga tctcgccgga ggcgctggag ccgcgtgtat
      601 tgcggaaggg cattttcacc aaagacgtgg aagatcaggc agccatagcc aaacgtctaa
      661 gccacgcagt gaacgatggc tttgcaggaa ccatcgctat ggcctctcag agcgcccaga
      721 acgcaaaaag agcgcgtgag ctacagaaga ctatggatgc gcagcagaaa cgcctacaga
      781 gcgtcacaga gccttttaag gggctttcca gagagcagat gaccgaaatc ctgatgatgg
      841 ctcagcggtt taagcagcaa aaccaggaaa aggaaaaaca gcagcgtatt gaacgtgaaa
      901 aacagcgaca gacgcgatcg cgtggcatgg gtggtatgga gcgttagttt tgatgcgatg
      961 caggataggg ctaacgccgc aaagtgacgt catttgcggc attcaccacg aaaaaattaa
     1021 tcattcagat ggtaattatg tttttttaaa atagaaaaag ctgcgttttt ctgctttggt
     1081 gacaaaccct tagttgagta tttattaccg ctagttctac gatatatgct gtaaacactg
     1141 cttaaaaaag tacgctccca aacagtcaga acctcattct tttttagtga atcatgcagc
     1201 attagcctgt ctacattttt aaaaagttta tcttcagcat tcatcatcca ttaccttcgt
     1261 ctgcaccgtt cttccattgg tgtcataact gatctttaac tcaattttct ttcttctttc
     1321 gtaaattgat ggccagtctc cccgtgcaaa aagaaagggg ttagcttcaa gttccccacg
     1381 ccctgcccgg aagaatattt gtttatctac aagagccgca attatgtttt taaaggtctg
     1441 atattcaata tgaaccctgg gctgtatggt tttttcaaat ctagtttttg atacagacac
     1501 aaacccatcc catccaatca gcattgccaa ttgtaaaagc acatcatttt gagattttga
     1561 taaaccatga atcatcgcaa tgtctgcaat gtatagcttg acgtagggag gttcggctgg
     1621 gatggaaaag gtctcgatct tagagtcttg ttttacctca ccgttttcat ccatgtatgt
     1681 atgccgttca agcctagaaa tctttttcgt attcactcca gccccacgtt tcgccatgat
     1741 ttagtactcg catttaagta ctaaagagac atacagtcaa gtcctttgta tcaagtacga
     1801 ttagtacttg aggtgagtac gattagtcct tgaggtgagt acgattagta cttgaggtga
     1861 gtacgattag tacttgaggt gagtacgatt cttcgctcta gcccttggta caagcgggtt
     1921 cccatatcta tatctatata ttaaggggga aaataagggc tgaaaaacgg catttttaga
     1981 cactgaaaaa ctgccttttt ttagcaagga gtgtacgcat ttctatgtac ggggagtgga
     2041 aattccgctt cctcgctttg gctgttcgtt cctcgtcgcg ttgctcctgc ggtacgcctc
     2101 agccaaagct gcggcgagcg gttcctgctc tttcaaagac gatttcattg cctgatttcg
     2161 tcaagggcga gcgtagcgag tgccatttac ccttgacgac actccggata aaacaggctt
     2221 ggcagtggtg gggggtggcg cgaagcgacg ccttccacct gccagaccgc cgttgatttt
     2281 gaccttctgg cagcttgctg cccgaacgat tttgcgtagc aaagtctagt gagtttacac
     2341 tatcatcttg atagtggccc gccggaggta tcgcaatgcg ctcccccgcc tggctgaatg
     2401 aatgccggaa tcagggggga acatggcgaa ctacgcgata atgcgctgca aaaagctgac
     2461 gggaatggga agcgtggcca gcgcgttgca gcactgctac cgagagcgtg agacgccgaa
     2521 cgctaacgct gaacggacac cagaaaacta ctgctctgtg tccaaatcaa cggatcaggc
     2581 aatggggcgt gtgcgggagc tgctgccgga aaaacgccgt aaagatgcgg tactggccgt
     2641 tgaatacgtt atgacagcaa gccctgagtg gtggaaagag gccacaccac ggcagcaggc
     2701 ggagtttttc gcccgctctg agcaatggct ggagaagaaa tacgggaagg atcgtgttgt
     2761 ggcggcggtg gttcaccggg acgaggccac cccgcacctg tctgcgttcg tggtgccatt
     2821 gacgcaggat ggacggctgt ccgcgaaaga gtttattggc gggcggtcaa agatgcgtga
     2881 agatcagagc acctacgctg aatcagtaaa aaaactgggt cttgagcgcg gcatcgaggg
     2941 aagccgcgcc acgcatcaga cagttcagca ctactacgaa tcgataaacc gtggcacccg
     3001 tagccaggta tcgatctcgc cggaggcgct ggagccgcgt gtattgcgga agggcatttt
     3061 caccaaagac gtggaagatc aggcagccat agccaaacgt ctaagccacg cagtgaacga
     3121 tggctttgca ggaaccatcg ctatggcctc tcagagcgcc cagaacgcaa aaagagcgcg
     3181 tgagctacag aagactatgg atgcgcagca gaaacgccta cagagcgtca cagagccttt
     3241 taaggggctt tccagagagc agatgaccga aatcctgatg atggctcagc ggtttaagca
     3301 gcaaaaccag gaaaaggaaa aacagcagcg tattgaacgt gaaaaacagc gacagacgcg
     3361 atcgcgtggc atgggtggta tggagcgtta gttttgatgc gatgcaggat agggctaacg
     3421 ccgcaaagtg acgtcatttg cggcattcac cacgaaaaaa ttaatcattc agatggtaat
     3481 tatgtttttt taaaatagaa aaagctgcgt ttttctgctt tggtgacaaa cccttagttg
     3541 agtatttatt accgctagtt ctacgatata tgctgtaaac actgcttaaa aaagtacgct
     3601 cccaaacagt cagaacctca ttctttttta gtgaatcatg cagcattagc ctgtctacat
     3661 ttttaaaaag tttatcttca gcattcatca tccattacct tcgtctgcac cgttcttcca
     3721 ttggtgtcat aactgatctt taactcaatt ttctttcttc tttcgtaaat tgatggccag
     3781 tctccccgtg caaaaagaaa ggggttagct tcaagttccc cacgccctgc ccggaagaat
     3841 atttgtttat ctacaagagc cgcaattatg tttttaaagg tctgatattc aatatgaacc
     3901 ctgggctgta tggttttttc aaatctagtt tttgatacag acacaaaccc atcccatcca
     3961 atcagcattg ccaattgtaa aagcacatca ttttgagatt ttgataaacc atgaatcatc
     4021 gcaatgtctg caatgtatag cttgacgtag ggaggttcgg ctgggatgga aaaggtctcg
     4081 atcttagagt cttgttttac ctcaccgttt tcatccatgt atgtatgccg ttcaagccta
     4141 gaaatctttt tcgtattcac tccagcccca cgtttcgcca tgatttagta ctcgcattta
     4201 agtactaaag agacatacag tcaagtcctt tgtatcaagt acgattagta cttgaggtga
     4261 gtacgattag tccttgaggt gagtacgatt agtacttgag gtgagtacga ttagtacttg
     4321 aggtgagtac gattcttcgc tctagccctt ggtacaagcg gg
//
