LOCUS       NZ_CP139289            11158 bp    DNA     circular CON 06-DEC-2023
DEFINITION  Acinetobacter pittii strain 564U plasmid pApi564Ua, complete
            sequence.
ACCESSION   NZ_CP139289
VERSION     NZ_CP139289.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38315702
            Assembly: GCF_034070325.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S., Guerrero,G., Garza-Gonzalez,E.,
            Lozano-Zarain,P., Volkow,P., Morfin-Otero,Md.R. and Cevallos,M.A.
  TITLE     Acinetobacter pittii: intercontinental dissemination, pathogenetic
            potential, and antibiotic resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S. and Miguel,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2023) Programa de Genomica Evolutiva, Centro de
            Ciencias Genomicas, Av. Universidad s/n, Col Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 564U_genome
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 840x
            Sequencing Technology :: DNBseq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/06/2023 00:11:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,877
            CDSs (total)                      :: 3,782
            Genes (coding)                    :: 3,701
            CDSs (with protein)               :: 3,701
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 37 of 81
            Pseudo Genes (incomplete)         :: 46 of 81
            Pseudo Genes (internal stop)      :: 21 of 81
            Pseudo Genes (multiple problems)  :: 19 of 81
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139289.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="564U"
                     /isolation_source="Bladder"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pApi564Ua"
                     /geo_loc_name="Mexico: Mexico city"
                     /lat_lon="19.4103 N 99.1511 W"
                     /collection_date="2015-02-20"
                     /collected_by="Hospital Infantil Federico Gomez"
     gene            316..1245
                     /gene="repM"
                     /locus_tag="SOI74_RS19325"
                     /old_locus_tag="SOI74_19325"
     CDS             316..1245
                     /gene="repM"
                     /locus_tag="SOI74_RS19325"
                     /old_locus_tag="SOI74_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005065049.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGFAPA"
                     /name="repM"
     gene            1324..1854
                     /locus_tag="SOI74_RS19330"
                     /old_locus_tag="SOI74_19330"
     CDS             1324..1854
                     /locus_tag="SOI74_RS19330"
                     /old_locus_tag="SOI74_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005065050.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSQNKIDLAEAIRV
                     FGEPVKKQDVKEPVKIDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPNMTTFTDQELVQPSIAKPDPEPDTAEAKHDGLTTPERRENKWIPVPEHIEPEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            2204..2521
                     /locus_tag="SOI74_RS19335"
                     /old_locus_tag="SOI74_19335"
     CDS             2204..2521
                     /locus_tag="SOI74_RS19335"
                     /old_locus_tag="SOI74_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000900860.1"
                     /translation="MLGKNHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            3042..3386
                     /locus_tag="SOI74_RS19340"
                     /old_locus_tag="SOI74_19340"
     CDS             3042..3386
                     /locus_tag="SOI74_RS19340"
                     /old_locus_tag="SOI74_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_005065055.1"
                     /translation="MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSGKKRFYTEMLAIADKQ
                     YELHLSTLGE"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3391..3693
                     /locus_tag="SOI74_RS19345"
                     /old_locus_tag="SOI74_19345"
     CDS             3391..3693
                     /locus_tag="SOI74_RS19345"
                     /old_locus_tag="SOI74_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_005065057.1"
                     /translation="MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            3785..4186
                     /locus_tag="SOI74_RS19350"
                     /old_locus_tag="SOI74_19350"
     CDS             3785..4186
                     /locus_tag="SOI74_RS19350"
                     /old_locus_tag="SOI74_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065059.1"
                     /translation="MTDKKQNDHLNLDGINSSYNDGDGLRINNPEDFRSITISNGYFSN
                     NKGNGITIGSPQQSPLEIILTQLAPKLPDTIQPYELASVIQNLLESTNQEEISQKLMTS
                     GLKEKFKDPNLWISFSSLLFSLIFQFSSK"
                     /name="hypothetical protein"
     gene            4238..4912
                     /locus_tag="SOI74_RS19355"
                     /old_locus_tag="SOI74_19355"
     CDS             4238..4912
                     /locus_tag="SOI74_RS19355"
                     /old_locus_tag="SOI74_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_005065062.1"
                     /translation="MSQKILQRYEQLLTDSASIKAMTKKAYSEYSGSYDTLGDEGDALY
                     LEWKVKVKNLLLLSCGEHSIHYRDFLDAEETQSFDTNTRIISRLIPILKASYDDFKNGF
                     LTSFKQIVQAEVFDSELEQARSLLSSGYKNSAAVIAGVVLETAIKELCLNNGIELEKKK
                     LTRLNEDLAKAGVYNPLQQKLITAMADIRNNAAHGDYDQFTKEDVHRMIEDIERFLLAY
                     SS"
                     /name="HEPN domain-containing protein"
     gene            complement(5003..5314)
                     /locus_tag="SOI74_RS19360"
                     /old_locus_tag="SOI74_19360"
     CDS             complement(5003..5314)
                     /locus_tag="SOI74_RS19360"
                     /old_locus_tag="SOI74_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005065064.1"
                     /translation="MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEI
                     TDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQGKGYQTRMNKILREAMLEDLKNH"
                     /name="BrnA antitoxin family protein"
     gene            complement(5301..5588)
                     /locus_tag="SOI74_RS19365"
                     /old_locus_tag="SOI74_19365"
     CDS             complement(5301..5588)
                     /locus_tag="SOI74_RS19365"
                     /old_locus_tag="SOI74_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002124950.1"
                     /translation="MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIISARQVTKAERNQYEHG"
                     /name="BrnT family toxin"
     gene            6101..7588
                     /locus_tag="SOI74_RS19370"
                     /old_locus_tag="SOI74_19370"
     CDS             6101..7588
                     /locus_tag="SOI74_RS19370"
                     /old_locus_tag="SOI74_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953322.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005065066.1"
                     /translation="MLSTIREQWFSNIRGDVLAGIVVALALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVIAFVGGRPGMISGATGAMALLMVTLVKEHGLQYLLAATILTGFIQILAG
                     YLKLGALMRFVSKSVVIGFVNALAILIFMAQLPELTNVTWHVYAMTIGGLAIIYLFPYI
                     PVIGKLLPSPLICIVLLTLFALFIGLDVRTVGDMGQLPDTLPIFLLPDIPLNLETLTII
                     LPYSLGLAAVGLLESMMTATIVDDLTDTNSDKNRECKGQGVANIASGFLGGMAGCAMIG
                     QSIINVKSGGGTRLSTFSAGVFLLIMVVFISDWLKAIPMAALVAVMIMVAIGTFNWDSL
                     RNIRQYPLSSNIVMIVTVIVVVATHNLAYGVLVGVLLSALFFANKIERYMAIQSEFNEP
                     ENTRTYTVTGQVFFSSADKFTSAFDFKEALSKVVIDVSQAHFWDVSAVAAVDKVVIKFR
                     REGTEVELIGMNEASKTLVDKFGIHDKPDALDRLNSH"
                     /name="SulP family inorganic anion transporter"
     gene            7601..8452
                     /locus_tag="SOI74_RS19375"
                     /old_locus_tag="SOI74_19375"
     CDS             7601..8452
                     /locus_tag="SOI74_RS19375"
                     /old_locus_tag="SOI74_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034565.1"
                     /GO_function="GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_005065068.1"
                     /translation="MSKIIACIDGSLATNTVCDYAAWFSDKLSSPLKLLHVIDKPKAKA
                     PQDLSGAIGLGSRETLLKELVEIEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSLLETIMGMEEDIRVLVMGKHGAETEHDSSKVGTHIENVVRALHKPVLITSSPFSPP
                     KSFLIAFDGSPTARICVDKIANSPLLKTLTAHVVYVGKPNSDMTLQVTWAKELLANNGF
                     DVIDSMLDGDVEKSILDYQEKNVIDMIVIGAYGHSKIRQFFIGSTTTKLLSSSTKPVLL
                     LR"
                     /name="universal stress protein"
     gene            complement(8723..9607)
                     /locus_tag="SOI74_RS19380"
                     /old_locus_tag="SOI74_19380"
     CDS             complement(8723..9607)
                     /locus_tag="SOI74_RS19380"
                     /old_locus_tag="SOI74_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_005065070.1"
                     /translation="MFYLNLKHNIKKEQSSLNAFHYLTRTAHFENQKDNEKLEFMRYGN
                     MPKWAENKPKLFWKSADQFEISRGRTSSTLTIALPKELILEQRAELVQKLIDQFAGQYQ
                     FPYTAVIHNHPSEITGEDQPHLHLMYSERTISDDIERPPEQFFKQYRPKNPTQGGAQKL
                     TADALGFGRNQIKVFREKTQELMNLFLEKYAPTKKVMVYGVEIEVKNQVSCLSNDDFNQ
                     KFGTKLQDVPQIPRWKLYSADPIIQLDVEAQKNTIKKIRNQNNAELYGKEYDLEISRRH
                     VLTQKKDPGFSLD"
                     /name="MobA/MobL family protein"
     gene            10063..10776
                     /locus_tag="SOI74_RS19385"
                     /old_locus_tag="SOI74_19385"
     CDS             10063..10776
                     /locus_tag="SOI74_RS19385"
                     /old_locus_tag="SOI74_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065071.1"
                     /translation="MAGRKTFALTQQEIIDLDKHIRALPFKRRMQDAFLAMISERESMK
                     QLNEEDLKLIQRCRYERNTYNKRIVQLQLIQKTEPQKLKKLELNILELSKKTDIDAYFA
                     MHDALNEVLKIKRNQTSEKNITAKVERVLNPEKYNQAPLSKSLRKQQDHIKYFIGSLYL
                     NLLEQSGLERSNSNEDLDRMLDLFKQAQSGAEKNNAFSLDQNRKTDILSAKSLYEMEKL
                     IKQAKEDPRNPFNKA"
                     /name="hypothetical protein"
     Typing          317..1232
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.884"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8727..9607)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagttcaata gagtttgagc gaagcgaaaa ccaagggcaa tttttcttta cctgggcttt
       61 taattatttt taatctttta aatgctttta agtaagctgt aaccctttat tcataatagt
      121 tttagagtta ataaaactag gtttatcgac ccataaaact aggtttatcg acccataaaa
      181 ctaggtttat cgacccataa aactaggttt atcgacctta aaactaggtt tatcgacccc
      241 aaattaagtc gatttacaat tcaattaaaa tagtctattg tacaacctag tttaattata
      301 aatttattat tagaaatgag tgaattaatc gtaaaggata atgctttaat tcaagctagc
      361 tataccctag atacagttga acaaaggctg atcctattag ccattgctga agctcgggaa
      421 acagggcacg ggataactga aaatagcctt ttagaagtac atgcaagtag ttatatcaat
      481 acctttaatg tcgagaaaca cactgcttat accgtgcttc gggatgcatc taaaagcttg
      541 tttgatcgct atgtcacgta tcatgacatt aatcctaaga ctggtaagga tcgtagcttt
      601 cactgtcgtt gggtcgataa aattggatat gaacctcaat ccgggatcgt tttcctacgt
      661 ttcacgcaag acattgttcc actcataact cgactggaag aaaactttac taaatatgaa
      721 ttggagcagg tttcgagatt aactagctct tatgctattc ggttatacga gttattaatt
      781 caatggagat ctgctggtaa aacgccaatt tttgatctat cgatctttag acaacaactt
      841 ggtgtcgaag ctcatcaata caaaacaatg agtaatttta aaacgtatgt tttagatttt
      901 gccctcaagc aagtaaatga gttaaccgat ataacggcta aatatgaaca acataaaaaa
      961 ggacgttcaa tttccggttt ttctttcagt tttaagcaga aaaaaaccaa ctctgacaaa
     1021 gtaattaagg gaacggatac attagccctt ttcaccaaaa tgagtgataa acaacgtcat
     1081 ctctttgcca acaaactttc tgaattacct gagatggggc aatattcaga aggcaccgaa
     1141 agcttccaac aatttgctat acgcattgca gaaatgctcc aagattatca aaaattccaa
     1201 gaattattcc cttatcttca aaaagttggc tttgcacctg cctaaaattt aaacttgatc
     1261 atttgtttat tagacagtct aatagacaac aacttttgac aggtttaggc atagatttac
     1321 aacatgaaaa gccttacagt tctcgagctt tctaagcttt acaatatcaa tagacaaacg
     1381 atttacaaca acataaagaa aggaatttta agtaaaaatt cccagaataa gatcgatcta
     1441 gccgaggcta tccgtgtttt cggggaacct gtcaaaaaac aggatgtaaa agaacctgta
     1501 aaaatagaca gtccaaattc ggcagaagtt ttactactta gacagcagat agacatgctg
     1561 aaaaaccagc ttgatgatgc caaagatcgg gaatctttct atcagaacca aattgaaacg
     1621 atgcaacgcc tattagaagc cccaaaacct aatatgacta cgtttaccga tcaagaatta
     1681 gtgcaaccca gcatagccaa accagatccc gaacctgaca cagcagaagc taaacatgac
     1741 ggattgacta ctccagagcg gagggaaaat aagtggattc cagtgccaga gcacattgaa
     1801 ccagagcctg agaaaagagg cttttggagc cgttttttta gaccctatga ttaaccacga
     1861 ttgtttaatt aatatgaata aagtcgtgaa actttgatta aataataagc gtggaacgtg
     1921 aaaaatcata aaaaaaaagc ccaccttggg gaggttgggc aaaaaagaaa ctacagcgtt
     1981 gatttctaag gaaaattata acacatttcg tataaggtgt attatgttaa taatataaat
     2041 ttttaaaagc atattgattt aataatagaa aaattatcag agtagggcat gtgtaaagta
     2101 aacgttcgat tattaaccaa taatgattaa agatttatat cttgtatgtg ttaattttat
     2161 taacatatat atacgaaatt aataatgcct taagtgaaat agtatgctag gtaaaaatca
     2221 tcatttttta aaaaaatttt cgatcagtga tgctgctata gcgtataaaa aacctcgctc
     2281 gactatactt aaatatataa aaagcggaga tttcgaactt gactacaata aattgattga
     2341 atacagtcag ttagttagaa aatttggtga acttacttac cctatacagg acgaaagaga
     2401 gcaacaaatc aaaaaactaa aagatgaagt tatgcattta aaaaaacaac ttaacttttt
     2461 taaatatact tcaatctcaa acaaaaatag actattaatt tctcataaac attccattta
     2521 acataataga gcttatacga aatgcttttt aaaatctcct tgtttttcat atagttaaaa
     2581 atttttcatc cccctaaaaa accacaaaaa accgactggg aaacaagtcg gtttcttttt
     2641 aagcaaaact gtaactttta gccaataaaa tcgatcaaaa aacattcgtt tttcattttt
     2701 tcctgatttc tcaatggtca cacttcacaa ataatcccca atttctgcgg acaactcttg
     2761 ggataaaata ggccaacttt gtaagctaaa gcttacagag atttaaggat tttgctgatt
     2821 ggtaatccaa ggtactagcc ctattatgaa tacataaggg aactggatgt ctccagacaa
     2881 caagaataag aaaatagcgc aaggaatggg cagcacgaga tcagtaagac gagcacccga
     2941 tatgaaaaag cccgacagga tgtcgggctt ttttattgcc atttgcaaag ttattcaatc
     3001 agctttaata tatagactat aatctatatt atggttgtaa tatgtggaca gtcattacaa
     3061 cggatctgtt taatgaatgg cttgaacagc aagatgaaat aacacaagag aaagtcttag
     3121 ctgcgttggt tgttctgcaa ctgcaaggac caagtttagg tcgtccctta gtagataccg
     3181 tctacgattc taaatttacc aatatgaagg aactacgtgt tcagcatcga ggtaaaccat
     3241 taagagcatt ttttgcattc gatccattac gacaagcaat tgtactgtgt attggtgata
     3301 agagcggtaa aaagcgtttt tatacagaga tgctagcaat tgcagataag caatatgaac
     3361 ttcatctctc aacattagga gaataatctg atggctaaga ccctacaaga attattggct
     3421 aatcgctctc ctgagagcca agcccgtatt caaaaaatgg ctgatgaatt gctattagaa
     3481 aatcagcttt accttattcg tgaagaactc gaaatctctc aaaaagaact ggctgaaact
     3541 ttgggaataa aacagccatc gctttcagca atagaaaatc gtggcaatga tcttaagatt
     3601 tcaaccatga aaaagtatgt tgaagctatg ggaggaaagc tccgtattga tgtggaactt
     3661 ccaactggga aacatatagg attcaatgta tagatataga aaagtgagtt taagctcact
     3721 tttttcattt ggtctgatca gtatgatata agttataaat cattcaaaag ataaaggctt
     3781 atatatgact gataaaaaac agaacgatca tctaaatctt gatggaatca actcatctta
     3841 taatgatggt gatgggctaa gaataaataa tcctgaggat ttcagaagta ttaccatatc
     3901 taatggctat ttctcaaaca ataaagggaa tggaattact attggctcac cgcaacaatc
     3961 accattagaa atcattctta ctcaattagc cccaaaactt ccagatacta ttcagcctta
     4021 tgaattggca tcagttattc aaaaccttct ggaatcaact aatcaagaag aaatatctca
     4081 aaaattaatg acttcaggcc ttaaagaaaa atttaaagat cctaatcttt ggatatcttt
     4141 cagtagttta ttgttttcat taattttcca attttcatct aaatagattt atagagttca
     4201 attacttaat ttggaaaccc taaaaaactg agtaaacatg agtcaaaaaa tattacagcg
     4261 ttatgaacaa ctactaaccg attctgcttc catcaaagct atgacaaaaa aagcttactc
     4321 agaatatagc ggtagctatg atactttggg tgatgagggg gatgctcttt atcttgaatg
     4381 gaaagttaaa gttaaaaatc tattattact atcatgtggt gaacattcta ttcattaccg
     4441 tgatttttta gatgctgaag aaacacagag ctttgatact aatactagaa taatcagtag
     4501 gttaattcca attttaaaag catcgtatga tgattttaaa aatggttttt taacttcttt
     4561 caagcaaatt gttcaagctg aagtttttga tagtgaatta gaacaagcac gatcgctgtt
     4621 aagtagtggt tataaaaatt cagctgctgt aattgctggt gtagttctag aaaccgcaat
     4681 taaagaacta tgtttgaata atggaataga attagagaag aaaaaattaa cacgattgaa
     4741 tgaagacttg gcaaaagcgg gagtttataa ccctctacag caaaaactaa ttacagctat
     4801 ggctgatatt cgaaataatg cggcccatgg agattatgat cagtttacaa aagaagatgt
     4861 tcacaggatg atcgaagata tcgaaagatt tctactagct tactcttcgt aagactgtca
     4921 gatttacata gaaaaaattt aattatttaa cataaaatct cttatgcgaa gcaaaaatgg
     4981 gagtctatag ctcccatttt catcaatgat tcttaagatc ttctagcatt gcttcacgca
     5041 gaattttatt catgcgtgtc tgatagcctt tgccttgagc ctttaaccat gcaagtacat
     5101 ctgcatctag acgaacagat gtttgttgct tcactggacg ataaaattgc ccatggcgaa
     5161 cagcatgact ccagtcggta atctcaggaa tgtcagatag atctaattgt tcatctggta
     5221 cagttccttt tgcaagcaag cgtttaattt cagcatcttg cttgtcactg aatttttcat
     5281 tcagttcttt gcgtgtatat ctaaccatgc tcatattgat tgcgttccgc tttagtgact
     5341 tgccttgcac taatgattcg tatgatctca cagtcatcat cgtcaatgat tgtatgagcc
     5401 accaataaca tcagtacacc tttaaccata ccgatcgttt gccaacgttc ttcaccattg
     5461 gtatgtcgat cctggattga gatccgtaaa ggatcttcaa aaacgaggct tgccgtttca
     5521 aaagatacat catgtttttt ctgattcttt tggtttttag cctcatccca ttcgaaatac
     5581 agttccataa aaaacattca aatttgtata tacaataatg tatcacaaat ttgtatcact
     5641 caagctaatt ggagtaattt ttagtcaaaa ttattggcaa gaagtatcaa acttgctcaa
     5701 aaaaaatccg ctttgtttcc aaagcggatt tttgcggtgg ttacgggtga tgtaaaatag
     5761 ttaagcatat gattttattt aacttataaa agacgatttc ggataacgcc cattatgtta
     5821 aatgggcata aatcaattct aaaattatct atgataattc ttagctttta cttcagtact
     5881 gcgtttaggg tggatagaaa aggggctagc tgctatgatt gcccccgaaa tcctccaaaa
     5941 tagatatctt tcttaatatg acacttacgc caccataagt ttatttacct cttaaatcag
     6001 taaataactc atcactgtct agtgatgtgg tgtagtagct tgtgtgtcgt atatatcaca
     6061 tcactagcct ttcttaaaat ttaaaaaaat aggctatttc atgttatcca ctattcgaga
     6121 acaatggttc tctaatattc gcggggatgt ccttgcgggt attgttgtcg ctttggctct
     6181 aattcctgaa gcaattgcct tctctattat tgcaggtgtc gatcctaaag tcgggttata
     6241 cgcttcattc tgtattgccg tggtgattgc tttcgtaggt ggtcgccctg gtatgatttc
     6301 tggggcaacc ggtgccatgg cgttattaat ggtaacactg gttaaagagc atggtctgca
     6361 atacttatta gcagccacaa ttttgacagg atttattcaa atcttagccg gctatctaaa
     6421 attaggtgcc ttgatgcggt ttgtatccaa gtcagttgtg attggctttg tgaatgccct
     6481 ggctattcta atttttatgg ctcaattacc tgaactcact aatgtgacat ggcatgtcta
     6541 tgcgatgaca attggtggtt tagccattat ttatctattc ccttatattc cagtgatagg
     6601 gaagctttta ccctctcctc tcatctgtat tgtcttactt acgctctttg ccttattcat
     6661 tggtttagat gtaagaaccg tcggagatat ggggcaatta ccggatacgc ttccaatttt
     6721 cttgcttcct gatattcctt taaatcttga aaccttaacg attattttgc cttattcact
     6781 gggattagca gccgtaggtt tgcttgaatc tatgatgaca gcaacgattg tcgatgattt
     6841 aacagatacc aatagtgata aaaaccgtga gtgtaaagga caaggcgttg ccaatattgc
     6901 ttcaggtttc ttaggcggaa tggcaggctg tgccatgatt ggtcaatcga ttattaatgt
     6961 gaaatctggt gggggtaccc gtttatctac atttagtgca ggggtctttc tgttaattat
     7021 ggtggtgttt atcagtgact ggcttaaagc cattccaatg gctgcactgg ttgctgtcat
     7081 gatcatggta gcaattggaa catttaactg ggattcacta cgcaatatcc gccaatatcc
     7141 cttatctagc aatattgtga tgatcgttac agtcatcgtg gtcgttgcga cacataattt
     7201 ggcttatggc gtattagttg gtgtacttct ttctgcatta ttttttgcca acaaaattga
     7261 acgctacatg gcaattcagt ccgaatttaa tgaacctgaa aatacccgta cttatacggt
     7321 aacaggtcaa gtgttcttca gctcagcaga taaatttact tctgcatttg atttcaaaga
     7381 agcactttca aaagttgtaa ttgatgttag tcaggctcac ttttgggatg tttctgcggt
     7441 cgctgccgta gataaagtgg tcattaagtt tagacgtgaa ggaacagaag tcgaacttat
     7501 tggaatgaat gaagccagca aaaccttagt cgataaattt ggcatccatg ataaaccaga
     7561 tgcattagat cgcttaaata gccactaaga gggatgaaaa atgtcaaaaa ttattgcatg
     7621 tattgatgga tcattagcaa caaataccgt ttgtgattat gcagcttggt ttagcgataa
     7681 acttagttca ccgcttaagt tattacatgt tattgataag ccaaaagcga aagcaccaca
     7741 agatttaagc ggtgcaatcg gcttgggaag tcgagaaacc ttgctcaaag aattggttga
     7801 aattgaagag cgtaagggga agattgaact agagcatggc caaattcttc ttcgggaagc
     7861 aaagaattat ctattggaga agttctctat tgatgctcaa agttttcaac gccacggcag
     7921 tcttttagag acaatcatgg gaatggaaga ggatattcgt gttcttgtta tgggtaaaca
     7981 tggggctgaa accgagcatg attccagtaa agttggaacc catattgaaa atgttgttcg
     8041 tgcacttcat aaacccgtat taataacatc ttcacccttt agtccaccta agtctttttt
     8101 aattgctttt gatggaagtc ctactgcacg tatttgtgta gacaaaattg caaatagccc
     8161 tttattaaaa accctgacag ctcatgtggt atatgttggt aagccaaata gtgatatgac
     8221 attacaagtc acttgggcaa aagagctact tgcaaataat ggttttgatg taattgatag
     8281 catgcttgac ggtgatgttg agaaatctat attggattat caagaaaaaa acgttattga
     8341 tatgattgta attggtgcat atggtcattc aaagatacgc cagtttttta ttggtagtac
     8401 gacgaccaaa ttgttatcaa gctctacaaa accagtatta ttattaagat aatttttaaa
     8461 agataaaagg atagttttta actatccctt ttttagattt tctaaaatta acataataca
     8521 ccttatgcga aaaacctaga ttttacattt atgggcaaaa atctaggttt tccttattga
     8581 cctgaatcaa aaacacaatc tatccatcat tgcagcctaa aaaacacaaa ttttccatgt
     8641 tgtggctaaa aatcactcac aatatggata agttttttca agattccatg ttcaaaaaac
     8701 acaaattttc cacttataaa aatcaatcta agctaaagcc aggatctttt ttctgagtaa
     8761 gcacatggcg tctagatatt tctaaatcat attccttgcc ataaagttca gcattgttct
     8821 gatttctaat ttttttaatt gtgttttttt gagcttcaac atcaagctga ataattggat
     8881 cagcgctata aagtttccaa cgagggattt gtggtacatc ctgtaatttg gtcccgaatt
     8941 tttgattgaa atcgtcatta gataggcaac tgacctgatt tttcacttca atttctacac
     9001 cataaaccat tactttttta gttggtgcat atttttctaa aaacaaattc attaattctt
     9061 gagttttttc acggaatact ttgatttgat ttcgcccaaa acctaatgca tcagctgtta
     9121 acttttgcgc accaccttga gttggatttt ttggccggta ctgtttaaaa aactgctctg
     9181 gtggtcgttc aatatcatct gaaattgttc tttctgaata catcaaatgt aaatgtggtt
     9241 gatcctctcc agtaatttca ctgggatgat tatgaatgac tgcggtataa ggaaattgat
     9301 attgtccggc aaactgatct attaacttct gaaccaattc tgctcgttgt tctagtatta
     9361 actcttttgg taaagcaatg gttaaagtag agcttgttct tccccgggaa atttcaaatt
     9421 gatcagcact tttccaaaaa agttttggtt tattttctgc ccatttgggc atatttccat
     9481 accgcataaa ctctaatttt tcattgtctt tttgattttc aaaatgtgcc gtccgagtta
     9541 aataatggaa tgcattcaga gaactctgct cttttttaat attatgcttt agatttaaat
     9601 aaaacatcag ttcacctcaa acaaattacg tgtaacgaaa cagcaaattt tcttgtaaca
     9661 aggggctaat tcttgttgca gagagcagtc tcttcttgtt gcaaacatcg atatttcatc
     9721 aaaaaagttt gttgcactcg tctaaaattc gctgttttcc ttgttgcacc cctttcccat
     9781 ttcttgtaac ggcagacatt gcttactttt tcaccattaa gaaaaatacc gttaaaaccc
     9841 gaagaaaaac tcataattta ataattaaat cagtggttta tgtagcctcc gcaggagcga
     9901 gtagaagtgt ctttttttaa caatgattgc taaagcaatc attgttaaaa aaagacacgg
     9961 aaacgagata aacaatattt tatgtataac ataaaatatt gtttcaacac catcagaatt
    10021 ccgctaacat caatttagat acccaaaatc aggaaatccg ctttggccgg tagaaaaaca
    10081 tttgccttaa cacaacaaga aatcatcgat ttggataaac atattcgagc gctgccgttt
    10141 aaaagacgaa tgcaagatgc ttttttagca atgatttctg aacgagaatc aatgaaacaa
    10201 ctgaatgaag aagatttaaa acttattcaa cgatgtcgtt atgagcgcaa tacttataat
    10261 aaacgtatag ttcaattaca actgatccaa aaaaccgagc cacagaaatt aaaaaaatta
    10321 gaattgaata ttctagaatt aagtaaaaaa acagatattg atgcttactt tgcaatgcat
    10381 gatgcattaa atgaagtttt aaaaattaaa agaaatcaga cttctgaaaa gaatattaca
    10441 gcgaaggtcg aacgagtttt aaaccctgag aaatataacc aggcccccct tagtaaatct
    10501 ttaagaaaac agcaagacca cattaaatat tttatcggat cactatattt aaacttattg
    10561 gaacaaagcg gtttagagcg atcaaattca aacgaagatt tagatcgaat gctcgattta
    10621 tttaagcagg cacaatccgg agcagaaaag aacaatgcat ttagcctgga tcaaaataga
    10681 aaaacagata ttttatcggc taaatcgcta tatgaaatgg agaaattgat taagcaagcc
    10741 aaagaagatc caaggaatcc tttcaataaa gcttagattg actcaaataa tagggtttaa
    10801 atacactgtt gaagccgtat atgccttttt tagcctaaac ctatattccc catcaataaa
    10861 aaaattagag tgctctaaac gcaaattaga gtattttgga gatcatttga ttttaccgat
    10921 aagaacaaag cgagtgtcta cgagcgatcc ttggaaaatt ttgcctagac tccctgaaaa
    10981 accgcttttt aggctgtgaa acctaaataa gcatggaaaa gttgtagaaa tttgagtcta
    11041 gatcgagcgt agcgagtaaa aaagttttat gagcgtagcg aattccgagt tgcttttgct
    11101 tttttaaagt cacgtaagtg ctcaccaaaa aattgcctcg acgaactgag cgaaagcg
//
