LOCUS       NZ_CP139285             4280 bp    DNA     circular CON 06-DEC-2023
DEFINITION  Acinetobacter pittii strain AE13 plasmid pApiAE13a, complete
            sequence.
ACCESSION   NZ_CP139285
VERSION     NZ_CP139285.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38315704
            Assembly: GCF_034073245.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S., Guerrero,G., Garza-Gonzalez,E.,
            Lozano-Zarain,P., Volkow,P., Morfin-Otero,Md.R. and Cevallos,M.A.
  TITLE     Acinetobacter pittii: intercontinental dissemination, pathogenetic
            potential, and antibiotic resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S. and Miguel,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2023) Programa de Genomica Evolutiva, Centro de
            Ciencias Genomicas, Av. Universidad s/n, Col Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: AE13_genome
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1601x
            Sequencing Technology :: DNBseq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/06/2023 00:45:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,068
            CDSs (total)                      :: 3,973
            Genes (coding)                    :: 3,848
            CDSs (with protein)               :: 3,848
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 37 of 125
            Pseudo Genes (incomplete)         :: 74 of 125
            Pseudo Genes (internal stop)      :: 22 of 125
            Pseudo Genes (multiple problems)  :: 7 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139285.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="AE13"
                     /isolation_source="Catheter"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pApiAE13a"
                     /geo_loc_name="Mexico: Mexico city"
                     /lat_lon="19.4103 N 99.1511 W"
                     /collection_date="2016-07-26"
                     /collected_by="Hospital Infantil Federico Gomez"
     gene            join(3657..4280,1..381)
                     /gene="repM"
                     /locus_tag="SOI75_RS20320"
                     /old_locus_tag="SOI75_20320"
     CDS             join(3657..4280,1..381)
                     /gene="repM"
                     /locus_tag="SOI75_RS20320"
                     /old_locus_tag="SOI75_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404019.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_032859646.1"
                     /translation="MLVVKDNALINASYNLELVEQRLILLAIIEARETGKGINANDALT
                     IHASSYIQNFNVQKHTAYGALKDACRDLFSRQFSYQELSPKGNVINQTSRWVSQIGYIQ
                     EEGSVQLIFAPAVVPLITQLEQQFTSYDLEQVSTLKSKYATRLYEILISWRSVGKTPMI
                     GLEELRGKLGLGVNDYKLLANFKLRVLELALEQINEHTDIFVKYEQHKKGRSIIGFSFK
                     FKQKQQSKIEKPIDQKRDPKTVDMFTGKTDAETKNIARAVVKDTSPPANPLLHRASRIT
                     TSIITHKLHERFKHGDEGLSSTLKRIQGEIMEGGEETADYWQSKLEDFGINFD"
                     /name="repM"
     gene            445..633
                     /locus_tag="SOI75_RS20325"
                     /old_locus_tag="SOI75_20325"
     CDS             445..633
                     /locus_tag="SOI75_RS20325"
                     /old_locus_tag="SOI75_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004676352.1"
                     /translation="MNTRFDPIVSEFESSDHEAEYNAWFIAKVEKSLADSRPTIPHDQV
                     VARMKKRREEREASANR"
                     /name="hypothetical protein"
     gene            620..892
                     /locus_tag="SOI75_RS20330"
                     /old_locus_tag="SOI75_20330"
     CDS             620..892
                     /locus_tag="SOI75_RS20330"
                     /old_locus_tag="SOI75_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676351.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004676351.1"
                     /translation="MLIVKWQPTAVEDLEAIIDYIANHNTVAAYDLEDHILQSAEDLAE
                     HPYKGRNGRRAGTRELLVHPNYWLVYEVSDLIRITNVIHTSRLYP"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(883..1185)
                     /locus_tag="SOI75_RS20335"
                     /old_locus_tag="SOI75_20335"
     CDS             complement(883..1185)
                     /locus_tag="SOI75_RS20335"
                     /old_locus_tag="SOI75_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005320422.1"
                     /translation="MTDEKEPKPKLTLEQKIEQQEQKLKQLKAQKQAAEAREKARKKEQ
                     DRKDDTRIKILLGSYLKKKMDSNPDFNSQILDELENYLTAERDRKLFGLSPLDQG"
                     /name="hypothetical protein"
     gene            1409..3022
                     /gene="mobQ"
                     /locus_tag="SOI75_RS20340"
                     /old_locus_tag="SOI75_20340"
     CDS             1409..3022
                     /gene="mobQ"
                     /locus_tag="SOI75_RS20340"
                     /old_locus_tag="SOI75_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_151766072.1"
                     /translation="MAIYHFSVKAISRGAGRSAVACAAYRSGEKLVCDYYGIEQDYTKK
                     SGVEFTNIYAPENTKKELLDRQKLWNAVEKSERRKDALLAREFEIAFPKELSQEQRQAL
                     LDELCQGIVKKHGVVVDAAIHVPHVGGGSDERNYHAHVMFTTRAIDKNGTFEAKKYRDF
                     SRDHGTKTVTEWRAAFADLVNAKLEQIGSKERVTHLSYADLGNGLEATQHEGYEVTQQR
                     RRYEAEQQKNEEDRDLAIIMPDVAMSNDAIKQRNADRYRDDQEIKGLDQEIIVSKAIVS
                     DLQQSKAKSDAEHAQQQQIALERKRERLEAQSLADAHKRATDFHTAFDPNHPDKSLVTQ
                     YITDYALLARHGKQPQPPAPVEQPQPTWWQRMTGQQPQQIEPPPFFEIDAIKAIVQSIV
                     EHEQKQAQQQKWQRLQEFLQHSPRTVDRLERDAISRSFDKGQQAGDEELAEFIKYHRQH
                     WQEKEQAAKDWQAEHDRFMREEAAKAREEAALTLQRERESEAKLEAYRERKAEIAEMQQ
                     KPVNRDEIQQQPRPKNDFEP"
                     /name="mobQ"
ORIGIN
        1 cagcataaaa aaggacgtag cattattggc ttttcattca agtttaaaca aaaacaacag
       61 tcaaaaattg aaaaaccgat tgatcagaag cgagatccaa agaccgttga tatgttcaca
      121 ggtaaaactg atgctgagac aaaaaacata gctagagcag ttgttaaaga cacctcaccg
      181 cctgcgaatc ccttgttgca tagggcaagt cgtatcacaa cgtcaattat cacccataaa
      241 ctgcacgaac gtttcaaaca tggcgatgag ggtttatcta gtacattgaa gcgtattcaa
      301 ggcgaaatta tggagggagg ggaggaaacg gcagactact ggcaatcgaa acttgaggat
      361 tttgggataa attttgatta aaaaataccc atgatttttg taccgtaaag cagtaaactt
      421 gagcgaataa ttggagtacc aactatgaat acaaggtttg atcctatcgt gtcagagttt
      481 gaaagttctg atcatgaagc agaatacaat gcgtggttta ttgcaaaagt tgagaaatca
      541 ctagccgatt cacgccccac cattccccat gaccaagtag tggcacgtat gaaaaaacgt
      601 cgtgaggaaa gagaagccag tgctaatcgt taagtggcaa ccaaccgcag tagaagattt
      661 ggaagcaata attgactata tcgccaacca taatactgtt gccgcatatg acctagaaga
      721 ccatattcta caatctgctg aagatttggc agaacaccca tacaaagggc gtaatggtcg
      781 aagagcagga acaagagagt tacttgtcca tccaaattat tggttagtct atgaggtttc
      841 agacctgatt cgcattacca atgtaataca tacgagtaga ctctatccct gatcaagagg
      901 ggatagtcca aacagttttc tatctcgttc ggctgtcaga tagttttcca gttcatctaa
      961 aatttgagaa ttgaaatctg ggttactatc catttttttc ttcagataag aaccgagtaa
     1021 aatttttata cgtgtgtcgt cttttctatc ctgctctttt ttacgtgcct tttctcttgc
     1081 ttcagcagct tgtttttgtg cctttagctg ctttagtttt tgctcttgct gttcaatttt
     1141 ctgttctaaa gttagctttg gctttggctc tttttcgtct gtcatgctct tttccaatat
     1201 caagtattct tctcgatcat aatcccgttt gaactggcgt tcaagacgtt atatgatact
     1261 caaagaatcc cgtaagggcg cacttagcca ttaatccata tacttcgtat ggattaatgg
     1321 tgcgaaaggg gaaacccccc tttaccccct gacaaatttc gttcctcaat ttgtatttcg
     1381 ctaaagcgaa ataagagcaa aagcaaatat ggcaatttat catttttcag tgaaagcgat
     1441 tagcagaggt gcaggacgta gtgccgttgc ttgtgctgcc tatcgttcag gtgaaaaatt
     1501 ggtatgtgac tattatggga tagagcagga ttacacaaaa aaatcaggtg ttgaatttac
     1561 aaatatttat gcacctgaaa acacaaaaaa agaattatta gaccgccaaa aattgtggaa
     1621 tgccgtagaa aaatctgagc gcagaaaaga tgctttactg gcaagagagt ttgaaattgc
     1681 ttttcccaaa gagttgagcc aggaacaacg gcaagcccta ctcgatgagt tatgccaggg
     1741 cattgtaaaa aaacatggtg tcgttgtaga cgcagcaata cacgtccccc acgttggtgg
     1801 tggatctgac gaacgtaact atcacgccca tgtcatgttt acgaccagag ctattgataa
     1861 aaatgggacg tttgaagcca agaagtaccg tgatttcagc agggatcatg ggacaaaaac
     1921 cgttaccgaa tggcgtgcag catttgccga cctagtaaac gcaaaactgg agcaaatcgg
     1981 ctctaaagag cgtgtaaccc atctgagtta tgctgacctt ggaaatgggc tagaagccac
     2041 gcagcacgag ggctacgagg ttacacagca aagaagaaga tacgaagcag aacaacaaaa
     2101 aaatgaagaa gatcgtgacc ttgccatcat tatgcccgat gttgcaatgt caaacgatgc
     2161 gatcaaacag cgcaacgctg atagataccg cgatgatcag gagattaagg ggctagatca
     2221 ggagattata gtctcaaaag cgattgtaag cgatttacag caatctaaag ccaaaagtga
     2281 tgcagaacat gctcaacagc aacaaatcgc tttagaacgc aaaagagagc gtttagaggc
     2341 acaatctcta gctgatgcac acaagcgagc tacagatttt catacagcgt tcgaccccaa
     2401 tcaccctgac aagtctttag taacccagta catcactgat tacgccttgc ttgcccgaca
     2461 tggcaagcaa ccgcaacccc ccgccccagt agaacaaccg cagccgacat ggtggcaacg
     2521 gatgacgggg caacagccac aacaaattga accaccgcca ttttttgaaa ttgatgcgat
     2581 caaagctata gtccagtcca ttgttgagca tgagcaaaaa caggctcaac agcagaaatg
     2641 gcaacggtta caagagtttt tacaacactc ccctcgcacc gtagaccgac ttgagcgaga
     2701 tgctatcagc agatcgtttg ataagggaca acaggcaggc gatgaagaac ttgccgagtt
     2761 catcaaatac catcgtcagc actggcagga aaaagagcag gcagctaaag actggcaagc
     2821 cgagcatgac cgctttatgc gtgaggaagc agctaaagca agggaagaag ctgcactcac
     2881 actacaaaga gagcgtgagt cagaagccaa acttgaagcg tacagagagc gcaaagcgga
     2941 aatagctgaa atgcagcaaa agcctgttaa tagggatgag atacaacaac agccacgccc
     3001 taaaaatgac tttgagccat agattattta ttttttctaa ataataagaa cagtttacag
     3061 agcgagtgtc tacgagcgaa taaccaaaat ttcgcccctt gcccctctga aaaaccgctt
     3121 tttacggttt tcgcccctag aaagtaacta ttttgtgtgc tgattttggc gtatctcatc
     3181 ttattaacag cccagaaccc tcgattttat tggatttcgt gcgcttttgc ttttcccaac
     3241 gccctagatg gtatttgcta aaaactgccc ctcagaccga gtgaagcgag gtcaaaatgc
     3301 cacgagcgaa gcgagtgtca tagggcagtt tttgtagtgc ttttgggctt ttaatgcttt
     3361 taatgctttt aaaagctttt agacgagctg aaagccttat ctagtaatga tttcagagat
     3421 tatatatata cgtttaccga cccatatata tacgtttacc gacccatata tatacgttta
     3481 ccgacccata tatatacgtt taccgaccat atatatacaa ataaattaat gttatatatt
     3541 tacataaatt tatttgtatg cctatataac atacaaggca agtatatgtc tcttggagta
     3601 ccaacaaaat gacctcaatc cagaaagcaa atgcaccacc acgaggaaca gccgatatgt
     3661 tggtagtcaa agataatgcg ttaattaacg ccagttataa tttagagtta gtcgaacaaa
     3721 gattgatttt attagcaatt attgaagcaa gagaaacagg aaagggaatt aatgccaatg
     3781 atgccttaac catccatgcc tcaagttata ttcaaaattt taatgttcaa aagcatacag
     3841 cctatggtgc gttgaaagat gcttgccgag acctattttc aaggcaattt agttatcaag
     3901 agttatcacc gaaaggtaat gttataaatc agacatcaag gtgggtgagc caaattggtt
     3961 atattcagga ggaaggtagt gtacaactaa tcttcgctcc tgccgttgtt ccacttatta
     4021 cacagttaga acagcagttt accagttacg acctagagca agtctcaact cttaaaagca
     4081 agtatgcaac tcgcttgtat gaaatactta tttcatggcg tagcgtaggc aaaacaccaa
     4141 tgatagggct agaggaactg agaggaaaac taggactagg tgttaatgac tataagcttt
     4201 tagcaaactt taaattacgg gtattagagc ttgcacttga acagattaat gaacatacag
     4261 acatttttgt aaaatacgag
//
