LOCUS       NZ_CP139284            10109 bp    DNA     circular CON 06-DEC-2023
DEFINITION  Acinetobacter pittii strain AE13 plasmid pApiAE13b, complete
            sequence.
ACCESSION   NZ_CP139284
VERSION     NZ_CP139284.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38315704
            Assembly: GCF_034073245.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S., Guerrero,G., Garza-Gonzalez,E.,
            Lozano-Zarain,P., Volkow,P., Morfin-Otero,Md.R. and Cevallos,M.A.
  TITLE     Acinetobacter pittii: intercontinental dissemination, pathogenetic
            potential, and antibiotic resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S. and Miguel,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2023) Programa de Genomica Evolutiva, Centro de
            Ciencias Genomicas, Av. Universidad s/n, Col Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: AE13_genome
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1601x
            Sequencing Technology :: DNBseq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/06/2023 00:45:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,068
            CDSs (total)                      :: 3,973
            Genes (coding)                    :: 3,848
            CDSs (with protein)               :: 3,848
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 37 of 125
            Pseudo Genes (incomplete)         :: 74 of 125
            Pseudo Genes (internal stop)      :: 22 of 125
            Pseudo Genes (multiple problems)  :: 7 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139284.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="AE13"
                     /isolation_source="Catheter"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pApiAE13b"
                     /geo_loc_name="Mexico: Mexico city"
                     /lat_lon="19.4103 N 99.1511 W"
                     /collection_date="2016-07-26"
                     /collected_by="Hospital Infantil Federico Gomez"
     gene            159..470
                     /locus_tag="SOI75_RS20250"
                     /old_locus_tag="SOI75_20250"
     CDS             159..470
                     /locus_tag="SOI75_RS20250"
                     /old_locus_tag="SOI75_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_005086328.1"
                     /translation="MEIEFSWDEHKAEINLKKHGVAFEDATLVFYDAHALYKQDRFENG
                     EYRWQAIGMVHGQAVLLVAHTVQDHNGMDHIRIISARQAEKKERKQYEQNRYLQNGFE"
                     /name="BrnT family toxin"
     gene            433..747
                     /locus_tag="SOI75_RS20255"
                     /old_locus_tag="SOI75_20255"
     CDS             433..747
                     /locus_tag="SOI75_RS20255"
                     /old_locus_tag="SOI75_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005086327.1"
                     /translation="MSKTVTYKMDLNNPPVLSEEQKTRLEALAKRPDSEIDFSDIPELD
                     ESFWKNAVQNPFYKPTKQVTTVRIDADVMQWLKAQGKGYQTRMNKILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            842..1369
                     /locus_tag="SOI75_RS20260"
                     /old_locus_tag="SOI75_20260"
     CDS             842..1369
                     /locus_tag="SOI75_RS20260"
                     /old_locus_tag="SOI75_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_000537572.1"
                     /translation="MGTVKLKSSDEVFQLKISLLGSDPEIWRTIQISSAATLSRLHKAI
                     QSAFDWEDSHLHEFTTIKHEKINKRQKLKEVLRVGKKIIYTYDFGDCWEHLITVESRQS
                     PDLNKKYPCCIDGQNHAPFEDIGGIFGYLDILDALQDPKHPRYDDYMQIIEDEIGEIEF
                     DPTYFNSHDIKF"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            1550..2161
                     /locus_tag="SOI75_RS20265"
                     /old_locus_tag="SOI75_20265"
     CDS             1550..2161
                     /locus_tag="SOI75_RS20265"
                     /old_locus_tag="SOI75_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_004740252.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLAAGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            complement(2236..2889)
                     /locus_tag="SOI75_RS20270"
                     /old_locus_tag="SOI75_20270"
     CDS             complement(2236..2889)
                     /locus_tag="SOI75_RS20270"
                     /old_locus_tag="SOI75_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005086345.1"
                     /translation="MYNVHPSEKLIHAFRQKPYQGCCPTQAEFLFIGLDANYAPNIEEQ
                     KIFSKIIEYHEDAISFWQKYNVHHPFLLDGYKGDGRKYHKEFSKIRLSCKDASRISFIE
                     LLHLPTTGRNDLKSSDLDKNHLQYIHKAIFSEHTKAVFISDAVFKLMKKTSIFSWMNDA
                     KQIEGHTLKVFHEKKPLIYKHLHFSTYGKFQAQKEEEIKAIHNIILKSNISDLT"
                     /name="hypothetical protein"
     gene            3117..3437
                     /locus_tag="SOI75_RS20275"
                     /old_locus_tag="SOI75_20275"
     CDS             3117..3437
                     /locus_tag="SOI75_RS20275"
                     /old_locus_tag="SOI75_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897309.1"
                     /translation="MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3430..3702
                     /gene="nadS"
                     /locus_tag="SOI75_RS20280"
                     /old_locus_tag="SOI75_20280"
     CDS             3430..3702
                     /gene="nadS"
                     /locus_tag="SOI75_RS20280"
                     /old_locus_tag="SOI75_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_004641921.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            4435..4764
                     /locus_tag="SOI75_RS20285"
                     /old_locus_tag="SOI75_20285"
     CDS             4435..4764
                     /locus_tag="SOI75_RS20285"
                     /old_locus_tag="SOI75_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="WP_004641881.1"
                     /translation="MINTEFSECISACTNCSLICTECATSCLKEKDLEMMRECIQRCLE
                     CADICQLCVRMQQHNSPFLKEICELCAKICEYCAEECEKHSHDHCKKCAEACRQCAEEC
                     RKIAM"
                     /name="four-helix bundle copper-binding protein"
     gene            complement(5015..5581)
                     /locus_tag="SOI75_RS20290"
                     /old_locus_tag="SOI75_20290"
     CDS             complement(5015..5581)
                     /locus_tag="SOI75_RS20290"
                     /old_locus_tag="SOI75_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_032024465.1"
                     /translation="MNTATKYSVSDAATLYKKSRATIYKDIKNGVLSRDYDGFIDFSEL
                     LRVYGEPFGAKQAKRIDTSSIQNQNTPEYTDKTVVELKNQIDFLKKQLEKAEDRESKAN
                     ARIDTLLTLIEMKPPTANMTTFTDQEPLQPSMAKTAPEPDTAEAKQDGLTTPEQTENKR
                     IPVPEQVEPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(5645..6568)
                     /gene="repM"
                     /locus_tag="SOI75_RS20295"
                     /old_locus_tag="SOI75_20295"
     CDS             complement(5645..6568)
                     /gene="repM"
                     /locus_tag="SOI75_RS20295"
                     /old_locus_tag="SOI75_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004775003.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_032066855.1"
                     /translation="MTNNNKLVVKDNALIDASFNLSLIEQRIMLLAIVGARESSNLTSD
                     TPIEVFVSDYVHQFKVDSNNAYGLLREAAKTLKRREFSYLDRYKGQEALTTANWVNKVT
                     YVDSSGLIVLYLSNEVISLISRLSEQFTKYHIEQVSDFKSKYSIRLYELLIKWLNVAKT
                     EKYSINDLRSKLGLDATEYSTMSNFKSNVLDRAISEINKHTNITVDYDQFKKGRVITDI
                     QFRIKSKDKPSQQELTKVSQTAFHQMTDAQINMFGNQLSRLPEFSNLANGNESYESLAA
                     KIKEMLRDPIQQKQFLPHLKNLGFKA"
                     /name="repM"
     gene            8019..8210
                     /locus_tag="SOI75_RS20300"
                     /old_locus_tag="SOI75_20300"
     CDS             8019..8210
                     /locus_tag="SOI75_RS20300"
                     /old_locus_tag="SOI75_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_199946769.1"
                     /translation="MSNFIVDSANIVSFPRPCDFCESKENVQKFAGLMLCGNCQENIRI
                     TNPGMFEANGQIEQKAQD"
                     /name="hypothetical protein"
     gene            8334..8960
                     /locus_tag="SOI75_RS20305"
                     /old_locus_tag="SOI75_20305"
     CDS             8334..8960
                     /locus_tag="SOI75_RS20305"
                     /old_locus_tag="SOI75_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005086335.1"
                     /translation="MIKEIVVFANSVKHGNSCVAGKCTRTGEWIRPVSNNEGAEIPVAQ
                     TKKINRRDNDSEWGLKVLNKIKVDLSHHAPLNHQTENYVINKTPWTDEFFIKKEEVTKY
                     LDSPQDLWGEGDRVSHDDIKDNQLIKNSLYLVQVHQLELFKDQNDKRRAKFSYGGVDYN
                     LAVTGREFDFHFKQNSTFLRNAIICVSLGENFNGHCYKLVAAIIS"
                     /name="hypothetical protein"
     gene            8974..9414
                     /locus_tag="SOI75_RS20310"
                     /old_locus_tag="SOI75_20310"
     CDS             8974..9414
                     /locus_tag="SOI75_RS20310"
                     /old_locus_tag="SOI75_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="WP_005086332.1"
                     /translation="MAIYTIGFTKKNAENFFTLLKNNQVKKVIDVRLNNVSQLAGFAKR
                     DDLIFFLREMCNCEYEHIPDLAPTEEILKPYKKGEIVWAQYEEKFMTLMAQRNIEKHIN
                     LLQFEDGCLLCSEHLPHQCHRRLVIEYLKKHNSGQHITVDLY"
                     /name="DUF488 domain-containing protein"
     gene            9504..10022
                     /locus_tag="SOI75_RS20315"
                     /old_locus_tag="SOI75_20315"
     CDS             9504..10022
                     /locus_tag="SOI75_RS20315"
                     /old_locus_tag="SOI75_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="WP_227520629.1"
                     /translation="MNFKITTNSSNELLKFYQEHNRDCIEVSYLPDWKNVEFTHAVIFK
                     DDEIFNDDEEYIKLLNIPLRVIQIKITRVINIDTEMQYKSIKKNDVYEYIGRGSPWGNP
                     YSMTTHDEEDRKNVIHSFKYDFDRDILLKAKKSDVYKLAGKRLGCFCKPFDCHGDVLAN
                     FLNSWDDGE"
                     /name="DUF4326 domain-containing protein"
     Typing          complement(5646..6568)
                     /name="rep_cluster_1283"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.225"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ataaaaaaat tgggacgcaa acccgaaaag ccgacttgga aacaagtcgg cttttttatg
       61 ttcgattctg taagcttttg ccaataaaaa atggaacaac tttgcaaaat ttacaatttt
      121 gtattaacat ttttatatta acaaaaagcg atgcaactat ggaaattgag ttttcatggg
      181 atgagcataa agcagaaata aaccttaaaa agcatggtgt agcttttgaa gatgcgacat
      241 tggtttttta cgatgctcat gccttatata aacaggatcg ctttgaaaat ggtgaatatc
      301 gttggcaagc gataggtatg gtacatgggc aagctgtttt attggtggcc cacacagtac
      361 aagaccataa tgggatggat catatccgca ttatttcggc acgacaggca gagaaaaaag
      421 agaggaaaca gtatgagcaa aaccgttact tacaaaatgg atttgaataa tcctcctgtt
      481 ttgtctgaag aacaaaagac aagacttgaa gccctggcta aacgaccaga cagtgaaata
      541 gacttttcag atattcctga acttgatgaa agtttttgga aaaatgcagt gcaaaatcca
      601 ttttacaagc cgactaaaca agtaactacg gttcgcatcg atgcagatgt catgcagtgg
      661 ctaaaagccc aaggcaaagg ctatcaaaca cgaatgaata aaattttacg ggatgccatg
      721 ctaaaagaat taaaaaatca tcaataaaaa tgggagctta ggctcccatt tttgactgcg
      781 tataaggtgt attatgttaa ttttagattt actaaaacta ttttttgaga actaggctgt
      841 tatgggaact gtaaagctca aatcttctga tgaagttttt caattaaaaa ttagtctgct
      901 aggtagtgat cctgaaatct ggagaactat tcaaatttca tcggcagcta ctttatctag
      961 gctccataag gcaattcaat ctgcttttga ttgggaagat agccatttac atgaattcac
     1021 gactattaag catgaaaaga tcaataaaag acaaaaattg aaagaagttc tacgtgtagg
     1081 taaaaaaatt atatatacat atgattttgg cgattgttgg gaacatttga ttactgtaga
     1141 aagcagacaa tctcctgatt taaataaaaa atatccatgt tgtatagatg gtcaaaatca
     1201 tgctccattt gaagatattg gaggaatttt tggatatctt gatattttag atgcattaca
     1261 agaccccaaa catccaagat atgacgatta catgcaaatt atcgaggatg aaataggaga
     1321 gatagagttt gatccgacct actttaattc acatgatatt aagttttaaa cctgatttaa
     1381 cataagggct gttatacgaa ttcccaaggc atggctttta gtcatgcttt ttttatcaaa
     1441 agagtatagc tgaatgatag cgagccaaat cgcatacaat atctattatt agacttaatg
     1501 taataattaa atgatagtgt tatcattaat ctaatagaat taagattaaa tgatagaaat
     1561 gaatacacgt atttatctac gagcttcaac taaagatcaa gatgcagaaa gggctttgca
     1621 gattcttcag gatctaaacc aaaacttgaa tttgggtgaa accattgtgt acgtagaaaa
     1681 ctatagtggt acgaagttag accgacctga attgaataag ctactgtcag aagcaaatca
     1741 aggtgacacc ttgttagttg aaagtattga ccgcttatca cgcctaaccc aacgagattt
     1801 tcaagagctg aagcgcagga tccaggagaa gggacttcgt ttagtcgtag cagatcttcc
     1861 tactacctac caaatgattc aaaccagtga cagcattact cattcaatcc tggaactcat
     1921 caacaatatg ttgattgatc tcctggcaac aatggctcgc ctagacaatg agaaacgtat
     1981 agaacgtatt aagcagggcc tggcacgttc aggttacaag ccaacaggta agaaagccaa
     2041 tgaggctaag cacaaacgaa taaaagaatt gctagcagct ggcaatatga ctaaggaaga
     2101 aattgccaaa gcagtgaatt gtggagttgc aactgtctat cgagttgcta aagttatcta
     2161 aaagcggaca tttttacttt ggagaaaccg gacatttcta ttttgaagtt acaagattgc
     2221 ttcacataag agattttatg ttaaatcact gatgtttgat tttagaataa tattatgaat
     2281 tgcttttatc tcttcctctt tttgagcttg aaactttcca taagttgaaa aatgtaaatg
     2341 cttatagatt aaaggttttt tttcatgaaa aactttaaga gtatgtcctt ctatttgttt
     2401 agcatcattc atccatgaaa atatagatgt ttttttcatt aatttaaaaa cagcatcaga
     2461 gataaataca gctttagtgt gttctgaaaa aatggcttta tgaatgtatt gtagatgatt
     2521 tttatcaaga tcagagcttt tcaaatcatt tctaccagtt gtgggaagat gaagaagctc
     2581 gataaatgaa atacgagaag catctttgca agaaagtcta atttttgaaa attctttatg
     2641 atattttcgc ccatctcctt tatatccatc taataaaaaa ggatgatgta cattgtattt
     2701 ctgccaaaat gaaatagcat cctcatgata ttcaatgatt ttagaaaata ttttttgttc
     2761 ttcaatattg ggagcgtaat tggcatctaa gccaataaat aaaaactctg cttgagtagg
     2821 acaacatccc tgataaggtt tttggcggaa agcgtgaatt agtttttcac ttggatgaac
     2881 gttatacata tttttatcag tagacgtaat gcaaagaata ttagtttatc cgatatagac
     2941 accaacatat taaaccgtcc gataagaaaa tcgcaaaagc gcatattcta aaagtggaca
     3001 tttttattgg tgagaatata gacattttga atgggttcta acagagagct gtaatctctc
     3061 gtttttttgt tctaaaactg ctaaaattct tatacgccat tgacgtactt gcacccatgt
     3121 tatttattga aaccagtatt tttaccaagc aaattaaaga gcttgtgagt gatgaagaat
     3181 atcgtcagct tcagcaagat ctcttggtac agcccgataa aggcgactta atcaagaatg
     3241 gcggtggtat tcgtaaagta cgttgtgctc aaggtaacaa gggtaaaagt ggtggtatcc
     3301 gggtgatcta ttattgggtc acagaggatg atcagatctt tttcctggtg gcttatccaa
     3361 agtctgtcaa agataatctg acagacaaag agacctcgat cctacgtcaa ttagtgaagg
     3421 agcaatttca tggataacaa cttatttgat gacctggttg cttcaattaa agaagctggg
     3481 gccatcaaac gtaatgagat aaaggcaagc cgcgtgactg agcttgaatt gccggatatt
     3541 aaagaagtac gtgagaaaac tggcttaacc caggcagaat tcgccgcacg tttgcatatc
     3601 agcgcgagaa ccctacaaaa ctgggaacaa ggccgtcgct atccgactgg tcctgcagcg
     3661 actttaattc gtattttaga tgctcatcca actttaattt aagaagttta gataataaaa
     3721 aatcccgacg tcatgtcggg atttttcggt atggagcgca taggtagggc tgactacctg
     3781 atggtcatgg tgcctgactg attattattg ttgttttaag tcattgaaca tcctgtccat
     3841 atagatcaat cataagagct tcatcggggg atagatacag acaatttcgt gaaacgctgt
     3901 aagccaaaac gtacaaaaaa taatcaaaat ttatcccaat ggttatccgc agaaattgtg
     3961 gattcttttg ggtaaaaaag gcttaaaaac tctatggaat tgctattttt gatcattatt
     4021 taaccaattt tattggcaag aagtatcaaa cttgctcaaa aaaaatccgc tttgtttcca
     4081 aagcggattt ttgcggtagt tttgggttat gtaaaatagc taagtacatg attttattta
     4141 acttataaaa gacgatttcg gataacgccc attatgttaa atagagggaa aatctatttt
     4201 taaagtgata aaattattgt agttatttta agaatagctg cttttgatac ttgatcatct
     4261 atcctaatta aaaactgttt tttaataatt tggaatataa ataagattta gttaaaatta
     4321 acattattca tataaattaa aattatgtat tacatttact aataatttaa aataattagt
     4381 agaaaaaaat tatgttttaa ttatttcttt gattatataa gttgagagta aaaaatgatt
     4441 aatacggaat tttcagagtg tatttctgca tgtacaaact gttcacttat ttgtactgaa
     4501 tgtgcaactt cttgtttaaa agaaaaagat ttagagatga tgcgtgaatg tattcaacgt
     4561 tgtctagaat gtgcagatat ttgtcagtta tgtgtgcgta tgcaacaaca taactcacct
     4621 ttcctcaaag agatttgtga gctttgtgct aagatctgtg aatattgtgc ggaagagtgt
     4681 gaaaaacatt cacatgatca ttgtaaaaaa tgtgctgagg cttgccgtca gtgtgcagaa
     4741 gaatgtcgaa aaattgccat gtaaaatact tcaataatgt taattatatg gctctagact
     4801 aacataatca ttctgctagt ctagagcctt tcctaaaatt aacataatac accttatacg
     4861 aaatgcgtta taattatcta tagaaatcaa tgctgtagtt tctagtttat agcccaatcg
     4921 ccaccgaatg ggcttttttt atgatttttc acgttccacg cctattcttt gatcaatgtg
     4981 tcacaacttt gattagcatg cctaaacaag tctcttagcc attcggcaag aaaaaccggc
     5041 ttaaccatcc tcttttcggt tgttccggtt caacctgctc cggcaccgga atacgcttat
     5101 tttccgtctg ctccggagta gtcaatccgt cctgtttagc ttctgctgtg tccggttctg
     5161 gagctgtttt ggccatgctt ggttgcagcg gttcttgatc ggtaaacgta gtcatattgg
     5221 ccgtgggagg cttcatttca atgagcgtta gaagcgtatc aatacgagca ttcgccttgg
     5281 attcacgatc ttcggctttt tctaactgtt tttttagaaa gtcgatttgg tttttcagct
     5341 ctaccactgt tttatcagtg tattcaggtg tattttgatt ttgtattgaa gacgtatcaa
     5401 tacgttttgc ctgttttgct ccgaaaggct caccatagac gcgaagtaat tctgaaaaat
     5461 ctataaaacc gtcatagtca cggcttaata caccgttttt tatatcttta tagattgtcg
     5521 ctcgggattt cttatacaga gttgcagcat cactaacact gtattttgta gctgtattca
     5581 tagtgtattg ctgtgtattt tgaaaacgta ttggagaata caatatgtgt attctctaat
     5641 acactcaagc tttaaagcca aggtttttta ggtgaggtaa aaactgcttt tgttgtattg
     5701 gatctcttag catttctttg atttttgctg ctaggctctc atagctttca ttaccatttg
     5761 ctaagttcga aaattcaggc aatcgtgaaa gctgattgcc aaacatattg atttgagcat
     5821 ctgtcatttg gtggaatgct gtttgggata cctttgttaa ttcttgttga gatggcttat
     5881 ctttcgattt aattctaaat tggatatctg tgataactcg ccctttcttg aattggtcat
     5941 agtcaacggt aatattagta tgtttattaa tttcacttat tgctctgtct aagacattag
     6001 atttaaagtt cgacatagtt gaatattcag tcgcatccag ccctaatttt gatctaagat
     6061 cgttaatact atatttctca gtttttgcta cattcaacca tttaattaat agctcatata
     6121 gtctgatgct atatttactc ttaaagtcag acacttgttc tatatggtat tttgtgaatt
     6181 gttcgcttaa tcggcttatt aagctgataa cttcgttact aaggtataaa acgataaggc
     6241 cactagaatc tacataggtc actttgttta cccaatttgc tgtagtaaga gcttcttggc
     6301 ccttatatct atctaaataa ctaaactccc ttctttttag agttttcgct gcttctctaa
     6361 gcaagccata ggcattatta ctatctactt taaattgatg aacgtagtca cttacgaata
     6421 cttctattgg tgtatcagat gtgagattag aagactctct agctccaact atggccaaga
     6481 gcataatacg ttgctctatg agtgataagt tgaaagaggc atcgataaga gcattatcct
     6541 taacaactaa tttattgtta tttgtcatga tatgaagctt attaattcat ttaactaggt
     6601 tatatgttat atattttaaa tagtcaataa tctagctatt caaatacccc ttaaacctat
     6661 ctttatcacc ccttaaacct atctttatca ccccttaaac ctatctttat caccccttaa
     6721 acctatcttt atcacccctt aaactattgc aaataaaggc ttacagcatc tctaaaagca
     6781 tttaaaaact taaaaataat taaaagcctt gggaatgaaa aattgccctt ggttttcgct
     6841 acgctcaaac tctattgaat ctcgctttcg ctcgattcgt ggggcaattt tttggatcat
     6901 tctgttgtga ctttgagaaa aagcaaaggc aagagcaaaa gcaactcgga attcgcttcg
     6961 ctcataaagc ttttttactc gctacgctcg atctagactc aattttctgc atgtgattta
     7021 cgcttattta ggcttcacag cctaaaaagc tgtttttcag agagtctagg tgcgaatttt
     7081 ggtgcttcgc tcgtagacac tcgctctatt catcccgatt agaataaaaa tagcctctat
     7141 ttggcgttca cttgcgtttt ttgacctgat tactcatttc aatagtgaga tctatgtctt
     7201 aagtaaaaat gtggctgaaa tcgcatttga gagcgtttca acttgggttt tatttttgat
     7261 ctgtaggcta gagtttccgc ttatagattt tttcccttag acatacaata gtcaggatct
     7321 cttttggcca agcactatct ttactgtcag ttaagtcaat taaaatggat tgggagccgg
     7381 tacataccgg caatttttgg ttcacacacc acgcatctaa agataaaggc ctaaaatccc
     7441 caatttataa gggatgcaga tgattgttta tgaggaagaa tccgatacca atagatagaa
     7501 gaaaaattat ttaagctttt gttttatata aacattcaat gataccgttc aagatatcaa
     7561 caaaatgcaa tttttagata ttaagtaatt gtttaatata taattttttt gatatcactt
     7621 tgatttttag tgtgatatca aaaataccta tttaaatttt aattatcaaa aacttaagtg
     7681 atatcaaagt gttctaacag tacataaaca gtgcttttgt catgtttacc agtcgttacg
     7741 atacaaaatt tagcccatga attggccatt tatatgctct agaaacgcgt actcaaaaaa
     7801 gaggtttttt tacgcttagt atttgataaa taggactatc tatgagtaca ctcatttgta
     7861 cgtgaaatag tggttaatgg ttcttaacat tctgttttta tttataaaat ttaagtacat
     7921 ctccttttct cctcatgtgt actcttagac actaaaaaac aactgtaagg ttctgaaaaa
     7981 tattgaaaat aatgagtact tattaggatt ctataattat gagcaatttt attgtcgatt
     8041 cagccaacat tgtttcattt cctcggccat gtgacttctg tgaatcaaag gaaaatgttc
     8101 aaaaatttgc agggctgatg ctttgtggaa attgtcagga aaacatccga atcactaacc
     8161 caggcatgtt tgaggccaat ggtcagattg aacaaaaagc ccaggattaa cctgggctgc
     8221 gtataagaga ttttatgtta aatagaaaga ctttggtcta tgaatataaa aatagtttcc
     8281 tggtaaataa ttaattataa tctgttgaaa taaaataaat tttggaagtt attatgatta
     8341 aagaaatagt agtattcgca aattctgtta aacatggaaa ttcatgtgtg gcaggaaaat
     8401 gtactagaac aggagagtgg attcgcccag tgagtaataa tgagggagct gaaatcccag
     8461 ttgcacaaac taaaaaaatt aatcgccgag ataatgattc agaatggggg ttgaaagttc
     8521 taaataaaat taaagtagat ctatctcacc atgcgccatt aaaccatcaa acagagaact
     8581 atgtaattaa taaaactcca tggactgatg agtttttcat aaaaaaagaa gaggttacaa
     8641 aatatcttga ttcaccccaa gatctttggg gtgaaggtga tagagtatct catgatgata
     8701 taaaagataa tcaactaatc aaaaattcac tctatttagt gcaagttcat caattagaat
     8761 tatttaaaga tcaaaatgat aaaagaagag caaagttttc ttatggggga gtagattata
     8821 atctagctgt tactggtcga gaatttgatt ttcattttaa acagaacagt acatttttga
     8881 gaaatgctat aatttgtgta agtcttgggg aaaactttaa tggtcattgc tacaaattgg
     8941 ttgctgcaat tatttcttag ttaacaggtt gttatggcta tatatactat tggttttacc
     9001 aaaaagaatg ctgaaaattt ttttactttg cttaaaaata atcaagtgaa aaaagtcatt
     9061 gatgtcagat taaataatgt ttctcaactt gcaggttttg ctaaaagaga cgatttaata
     9121 ttttttttac gtgaaatgtg taattgtgag tatgaacata tacccgattt agcgcccaca
     9181 gaagaaattt taaaacctta taaaaagggt gaaattgtct gggctcagta cgaagaaaaa
     9241 tttatgacat taatggctca aaggaatatt gaaaagcata tcaatctttt gcaatttgaa
     9301 gacggttgct tgctctgtag tgagcattta ccccatcaat gtcatcgtcg attggttatt
     9361 gaatatttaa agaaacacaa tagtggacaa catattacag tggatcttta ttaattatga
     9421 atcaccttct agttctttta cctgaagagt ttaactgtta taccaaatta gaaagaaagt
     9481 tagatcggat cagtagtagt ctaatgaact ttaagattac tactaatagt tcgaatgagt
     9541 tgctaaagtt ttatcaagag cataatagag attgcattga agtctcttat cttccagatt
     9601 ggaaaaatgt agagtttaca catgctgtaa tttttaaaga cgatgagatt tttaatgatg
     9661 atgaggaata tattaaatta ttaaatattc ctctaagagt tatccaaata aaaattacta
     9721 gagttattaa tatagatacc gagatgcagt ataaatctat taaaaaaaat gatgtatatg
     9781 aatatatcgg tcgtggatct ccttggggaa atccatactc tatgactact catgatgagg
     9841 aagataggaa aaatgttatc cattctttta aatatgactt tgatagagat attttattga
     9901 aagcaaaaaa aagtgatgta tacaaattag ctgggaaaag attagggtgc ttttgtaagc
     9961 catttgattg tcatggtgat gttttggcta attttttaaa tagttgggat gatggggaat
    10021 aaagaattat ttatttttcc gaaaattaac ataatgggcg ttatccgaaa cgcatataaa
    10081 aatagtttta tatttcaaaa agttatgct
//
