LOCUS       NZ_CP139278            10491 bp    DNA     circular CON 06-DEC-2023
DEFINITION  Acinetobacter pittii strain AN37 plasmid pApiAN37d, complete
            sequence.
ACCESSION   NZ_CP139278
VERSION     NZ_CP139278.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38315705
            Assembly: GCF_034072545.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S., Guerrero,G., Garza-Gonzalez,E.,
            Lozano-Zarain,P., Volkow,P., Morfin-Otero,Md.R. and Cevallos,M.A.
  TITLE     Acinetobacter pittii: intercontinental dissemination, pathogenetic
            potential, and antibiotic resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S. and Miguel,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2023) Programa de Genomica Evolutiva, Centro de
            Ciencias Genomicas, Av. Universidad s/n, Col Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: AN37_genome
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 11242x
            Sequencing Technology :: DNBseq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/06/2023 00:32:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,856
            CDSs (total)                      :: 3,761
            Genes (coding)                    :: 3,675
            CDSs (with protein)               :: 3,675
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 40 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 23 of 86
            Pseudo Genes (multiple problems)  :: 10 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139278.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="AN37"
                     /isolation_source="Peritoneal Dialysis Fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pApiAN37d"
                     /geo_loc_name="Mexico:Puebla, Puebla"
                     /lat_lon="19.0344 N 98.2447 W"
                     /collection_date="2014-11-17"
                     /collected_by="Hospital del nino Poblano"
     gene            1..936
                     /gene="repM"
                     /locus_tag="SOI78_RS19140"
                     /old_locus_tag="SOI78_19140"
     CDS             1..936
                     /gene="repM"
                     /locus_tag="SOI78_RS19140"
                     /old_locus_tag="SOI78_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237873.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_006582659.1"
                     /translation="MPTSELIVKDNALINASYNLDTVEQRLILLAILQARETRTGIDAN
                     TRLRIHASDYMSRFNVNKHAAYKALKTAVTNLFGRQFSYQTIDEKTGKSKKVISRWVQN
                     ISYIDDAATLEVTFTMDVVPLITRLEKQFTSYQLKQVTQLTGKYAIRLYELLIAWREVG
                     KTPIFEVSDFRSKLGLTSDDYPRLDTFKRRVLESAVKQINEHTDIIVKVEQHKEGRSIS
                     GFSFSFKQKRNPNKVIERKKDPNTLDIFSRITDAQRHLFANKLSELPEMGKYSQGTESY
                     QQFAIRIAEMLQDPAKFQELHPYLEKVGYR"
                     /name="repM"
     gene            1005..1550
                     /locus_tag="SOI78_RS19145"
                     /old_locus_tag="SOI78_19145"
     CDS             1005..1550
                     /locus_tag="SOI78_RS19145"
                     /old_locus_tag="SOI78_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_025466892.1"
                     /translation="MKALSVIELSQLYGMNRQSIYKRINKGDLSKNSDGKIDLSEAIRV
                     FGEPSNKNNNVTTLQSTTVQKDTEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKTNMTTFTDQKPSQDIVTNPRPESEPKHDVLTTPGQTENKRIPVPEHI
                     EQEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(2064..2222)
                     /locus_tag="SOI78_RS19150"
                     /old_locus_tag="SOI78_19150"
     CDS             complement(2064..2222)
                     /locus_tag="SOI78_RS19150"
                     /old_locus_tag="SOI78_19150"
                     /inference="COORDINATES: protein motif:HMM:NF013677.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_006582642.1"
                     /translation="MDLKTVIDTAPTLKKPRRTFTAEFKHQLIQQCQQPDTSVAKVAMQ
                     HQIKRWF"
                     /name="transposase"
     gene            2481..3431
                     /locus_tag="SOI78_RS19155"
                     /old_locus_tag="SOI78_19155"
     CDS             2481..3431
                     /locus_tag="SOI78_RS19155"
                     /old_locus_tag="SOI78_19155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002058606.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_006582641.1"
                     /translation="MSLFYENFRDFEDAGKAILEYLHNHLVFNLWMITCTEGNDWIVLQ
                     AKDSGYGVKAGQVFNWADSYCSKMVLGKGPRIAHDSKKIPLYEEAGINKVVDINAYIGQ
                     PLLNEDGSVFGTLCAIDPSVQPKSIEQYSDLLELFSLLLSRILQNEIKINEQKRISEKL
                     EMESLTDHLTGAFNRRAWDKFLDLEESRCKIYGHSTTIIVIDLNDLKYINDKFGHLKGD
                     QMIQETVQIIKRTVRSNDLVARLGGDEFGILNIETNLENTQKLANRISESLKQAGINAA
                     IGVAARQPGTDLQQTVVEADKQMYRHKRQIKDMSQ"
                     /name="GGDEF domain-containing protein"
     gene            3691..3978
                     /locus_tag="SOI78_RS19160"
                     /old_locus_tag="SOI78_19160"
     CDS             3691..3978
                     /locus_tag="SOI78_RS19160"
                     /old_locus_tag="SOI78_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_023271598.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDRYEHG"
                     /name="BrnT family toxin"
     gene            3965..4279
                     /locus_tag="SOI78_RS19165"
                     /old_locus_tag="SOI78_19165"
     CDS             3965..4279
                     /locus_tag="SOI78_RS19165"
                     /old_locus_tag="SOI78_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005098515.1"
                     /translation="MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            4362..4697
                     /locus_tag="SOI78_RS19170"
                     /old_locus_tag="SOI78_19170"
     CDS             4362..4697
                     /locus_tag="SOI78_RS19170"
                     /old_locus_tag="SOI78_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025466893.1"
                     /translation="MKIGIFWFLQKQVIGIAHPFNLNDADSIGLIDSPYTHVDYWKNMQ
                     SVYPELRHYEYEQIPRGRVVFDANKEKAIVYMDKKLFNTVTATKIYDFFDIDSENAIPR
                     KDPHYRT"
                     /name="hypothetical protein"
     gene            4747..4926
                     /locus_tag="SOI78_RS19175"
                     /old_locus_tag="SOI78_19175"
     CDS             4747..4926
                     /locus_tag="SOI78_RS19175"
                     /old_locus_tag="SOI78_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001231652.1"
                     /translation="MRKRLKDYSLDQISNREKVSGQVLPIQFSNDEATKRVVMHSAKRV
                     INQHKAEIQELAYK"
                     /name="hypothetical protein"
     gene            4923..5309
                     /locus_tag="SOI78_RS19180"
                     /old_locus_tag="SOI78_19180"
     CDS             4923..5309
                     /locus_tag="SOI78_RS19180"
                     /old_locus_tag="SOI78_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769969.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_000053135.1"
                     /translation="MSLISSTFVIAVHDEILLQTGVGRIGCHIEKLESVLSRIDQQMYY
                     SNVSDIFEIAAWFGIAISKGHAFVDGNKRTGLAVMLTYLEIQGVTIQEQTGLDDLMVDI
                     VESQEEHELLAKIVAEFLYELSII"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(5311..5862)
                     /locus_tag="SOI78_RS19185"
                     /old_locus_tag="SOI78_19185"
     CDS             complement(5311..5862)
                     /locus_tag="SOI78_RS19185"
                     /old_locus_tag="SOI78_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="WP_000755487.1"
                     /translation="MKKVHLIQADITAFAVHAIVNSANKSLLGGGGLDYVIHKKAGPLM
                     KEECVRLNQEKGGCPTGQAEVTTAGNLPAKYLIHAVGPRWLDGEHNEPQLLCDAYSNAL
                     FKANEIHALTVSFPCISTGVYGFPPQKAAEIAIGTILSMLPQYDHVAEVFFICREDENY
                     LIYKNLLSNIDDPNIQILIS"
                     /name="macro domain-containing protein"
     gene            complement(5871..6569)
                     /locus_tag="SOI78_RS19190"
                     /old_locus_tag="SOI78_19190"
     CDS             complement(5871..6569)
                     /locus_tag="SOI78_RS19190"
                     /old_locus_tag="SOI78_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006582673.1"
                     /translation="MERNLNLANSIFEGFNDKNGLMICGYEWGWSKEDQKLDESDSRPT
                     VDMSIECTFSNKSLRYGPSSNTWPYDKNIKKWFNFWGHPLNDENLGSDFDKCIIQTNWA
                     YTSNADIESYNRFLEKEAVDNFIHHIEVLRPKVILFMGSKLINFLRNIDVWDRFTAIVG
                     PEIEPLKMVQKTDFDGTRFKVYFDNFEQCQVVCLPHPSSSRGLSDEYIKLFEPELGTII
                     TEYKKQKGIL"
                     /name="hypothetical protein"
     gene            complement(6877..7122)
                     /locus_tag="SOI78_RS19195"
                     /old_locus_tag="SOI78_19195"
     CDS             complement(6877..7122)
                     /locus_tag="SOI78_RS19195"
                     /old_locus_tag="SOI78_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_006582672.1"
                     /translation="MPYEEFHRLIGKAGLSIKEFAELLGMRPNSITNYNKVGFVPSHLA
                     VIVSLISTMKDEGFDFYPILEKVQAYEKARIESQSK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            7275..7517
                     /locus_tag="SOI78_RS19200"
                     /old_locus_tag="SOI78_19200"
     CDS             7275..7517
                     /locus_tag="SOI78_RS19200"
                     /old_locus_tag="SOI78_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006582671.1"
                     /translation="MNYFTQPQLFISAKAKKLGLQLIQELYIQRSTRLAFYLDVNPSFD
                     GHEAVLFWTEHYLAEYPDANFSDIQQQFIQAVPQS"
                     /name="hypothetical protein"
     gene            complement(7684..8445)
                     /locus_tag="SOI78_RS19205"
                     /old_locus_tag="SOI78_19205"
     CDS             complement(7684..8445)
                     /locus_tag="SOI78_RS19205"
                     /old_locus_tag="SOI78_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006582670.1"
                     /translation="MTQEKAKKRGRPSQLLQMAELHAFVEFLLEKDPRSELQNQVIDAL
                     QAEDFNFEMLSEAQQILVKEALKPYREHLKLQLLFDELSQNFQHTEYEEKFMELYQGYK
                     QDGLDPVEMNILKMMCTRYLNFKAQKLEYSDLELYLGQIQKKNASEKRKAENKRKFELG
                     GAVLAAFKKLNLDISKETPEQITNRIVNNKVFHDEVKKSKIFSEVSKYKKEYFERNKLF
                     IDVLEGLHTWKKEGMTLTQIEIEKVLRKNNE"
                     /name="hypothetical protein"
     gene            8750..9925
                     /locus_tag="SOI78_RS19210"
                     /old_locus_tag="SOI78_19210"
     CDS             8750..9925
                     /locus_tag="SOI78_RS19210"
                     /old_locus_tag="SOI78_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_006582669.1"
                     /translation="MASYHFSVKSKNKGYAMGHYLYIARLMQYENVRKKSDELLESVEL
                     GKCMPSWVKDPIEFWQAADTYERANAKVYMEYEIALPNEFTPDQRKTLIETFLDKHIAP
                     QQYPHSYAIHNVKSRLSGEEQPHCHLMFSLKADDGIERTAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHADYSTFLLHIRKAWENHLNDALAEHCPTVTYQINGQDITIKNQVSADNYEKY
                     NEIHGTLYLPEPKLGAGKQNETVEYLVEIQKIRQHNANEREMEQKQQVLKHEPFYSCLN
                     EPYNSLEVMYYLNHLAKKAKNKKELEQDFNEIIKTQKPKLQDEHWNLSRMDNQLQNSLC
                     YPDPQVDFKPLIGLKLQMEAKPVIQENKVSETIRPSTQPKPKNNDYDFGGP"
                     /name="MobA/MobL family protein"
     Typing          2..936
                     /name="rep_cluster_1233"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.893"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8751..9922
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.952"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcctacca gtgaattaat agtaaaagat aacgctttaa ttaatgcgag ttataattta
       61 gataccgtag aacaacggtt aattctactg gctatcttac aagcaagaga aacaagaaca
      121 gggattgatg caaacactag gctacggatc catgccagtg actatatgag cagatttaat
      181 gttaataagc atgccgccta caaagcccta aaaacagcag ttactaacct ttttggtaga
      241 cagtttagct atcaaacaat tgatgaaaaa acaggaaaat caaagaaagt aatttctcga
      301 tgggttcaaa atatttctta tattgatgat gctgcaacct tagaggttac ttttacaatg
      361 gatgtagtac ccttaattac acgtttagaa aaacaattta cgagttatca gttaaaacaa
      421 gttacccaat taacaggaaa atatgccatc cgactctatg agttgcttat agcatggcgt
      481 gaagtcggta agactcctat atttgaagtt tctgactttc gttcgaagct tggcttaact
      541 tcagatgatt acccaagact agatacattt aaacgacgtg tacttgaatc agcagtcaaa
      601 cagataaatg aacatacaga tatcatcgtt aaagttgaac agcataaaga aggtcgctct
      661 atttctgggt tctcttttag cttcaaacaa aaaagaaatc cgaacaaagt aatagaacgc
      721 aagaaagatc ccaatacatt agatattttt tccagaataa cagatgctca acgtcaccta
      781 tttgctaaca agctttcaga attacctgaa atggggaaat actctcaggg cactgaaagc
      841 tatcaacagt ttgcaataag aattgctgaa atgcttcaag atcctgctaa gtttcaagaa
      901 ttacatcctt atcttgaaaa agttggctat cgttaaacta ctgatgtaac gttacatgat
      961 taattattgt ctacaagcat acagattttt acttgggtta catgatgaaa gcactatctg
     1021 ttattgagtt atcacaactt tatgggatga atcgacaaag tatttataag cgtataaata
     1081 aaggagattt atcaaaaaat agtgatggga aaatagattt atctgaagcc atacgagtat
     1141 ttggcgaacc atctaataaa aataataatg taactacgtt acagtctact acggtacaaa
     1201 aagatacaga agtagacatg ctaaaacagc aagtagacat acttaaaaaa cagctagaac
     1261 ttgcacatga gcgagaagtt ttccaaagag aacagctaga agcaaaagac aatcaaatag
     1321 aagcaataca acgactgcta gaagccccaa agactaatat gactacgttt accgatcaga
     1381 aacctagcca agatatagta actaatcctc ggccagaatc tgagccaaaa catgatgtat
     1441 tgactacgcc ggggcagact gaaaataagc ggattccagt accggagcac attgaacagg
     1501 aaccggaaaa aagaggcttt tggagccgtt tttttagacc ctatgattaa ccacgattgt
     1561 ttaactgata tgaacaaagt tgtggaacat tgatcaaaga ataggcgtgg aacgtgaaaa
     1621 atcataaaaa aagcccgaac agtgatgctc gggctataaa ctttgaaata agtgtatgaa
     1681 tttcaaggta aattataaca cgtttcgtat aaggtgtatt atgttaattt tagaaaaatt
     1741 aaacttgtaa aatattaaca taactgtctg acatttcttg cagatctaac ttagtagtaa
     1801 catttagaat gtcatcgtaa ttcagaagat tttgtattgg aaaaataccc ttaagtattg
     1861 atctaagaag gaaataataa gaatagagct gttcatgaat atcaaagttg atatcagcgc
     1921 ctctaataaa atcataacca atatcgacat ttctatcatc tcgataaatg gcattggcta
     1981 aatcttggaa atcgttacct aagtcaaagt ataccaatgg tacttcatat ttagatctag
     2041 ctatatcttt tcgatataca tagttaaaac catctcttga tctgatgttg cattgcgacc
     2101 ttagccaccg atgtatctgg ctgctggcat tgctgaataa gttgatgttt aaattcagcc
     2161 gtgaaggttc gacggggctt ttttagcgtt ggtgcagtgt ctataactgt ctttaaatcc
     2221 atagataggt gtccacttaa attttagtgg acactatcgc ggtattaatg agttagaaaa
     2281 agtggggatc agcggacgct tacgttgtta actaaaaagt tatattttct atatagagtc
     2341 gtttaagaat atatcgtaaa aaatattaag aaaaataaat tttacgatat attatatcta
     2401 tgaaattttt aaattaatct ttaaatacaa tagtttaaat aaagttgtat aaatttttaa
     2461 agtattgata gtgagtagtt atgagtcttt tttatgaaaa ctttagagat tttgaagatg
     2521 ctggtaaagc cattttagag tatttacata atcacttagt ttttaattta tggatgatta
     2581 cttgtacaga aggtaatgat tggatagttc ttcaagctaa agacagtgga tatggagtaa
     2641 aagcaggaca agtttttaat tgggctgatt catattgttc taagatggta ctaggcaaag
     2701 ggccacgtat agcacatgat tcaaaaaaaa ttccattata tgaagaggct ggaattaata
     2761 aggtagtgga tataaatgca tatataggtc aacctttatt gaatgaagat ggctctgttt
     2821 tcggtacatt atgtgctatt gatccatcag tacagcctaa aagtatagaa caatatagtg
     2881 atttattaga attatttagt ttattactca gtcgaatttt acagaacgaa attaaaataa
     2941 atgaacaaaa aagaattagc gaaaaacttg aaatggagtc gctaacagat cacttaacag
     3001 gagctttcaa tcgtcgtgct tgggataaat ttttagattt agaagaaagt cggtgtaaaa
     3061 tttacggaca ctcaacaaca attattgtca tagatttaaa tgatttaaaa tatataaatg
     3121 ataaatttgg tcatttaaaa ggtgatcaga tgattcaaga aacagtccaa attatcaaaa
     3181 gaactgtgcg ctctaatgat ttagttgcac gtttaggagg agatgagttt ggtattttaa
     3241 atattgaaac taacttagaa aatactcaaa aactagcaaa tcgaatttca gaatccttaa
     3301 aacaggctgg aattaatgct gctattggtg ttgcagcacg tcaaccaggg acggatttac
     3361 aacaaacagt agttgaagca gataagcaaa tgtatagaca taaacgtcaa atcaaagata
     3421 tgtctcaata aggtaataaa attaagtttt cctacaatta acataatggg cgttatacga
     3481 aacgcatata aaaatagttt tatatttcaa aaagttatgt tataaaaaat cggggacgca
     3541 aaccctaaaa gccgacttgg aaacaagtcg gcttttttat gagcagtttt gatagttctt
     3601 gccaataaat ctgactaaaa attacgcttt tcagcttgag tgatacaatt ttgtgataca
     3661 ttattgtata tacaaatttg aatgtttttt atggaacagt atttcgaatg ggatgaggca
     3721 aagaatcgaa agaatcagaa aaagcacgat gtctctttcg aaacagcaag ccttgttttt
     3781 gaagatccac tgaggatctc aatccaggac agacataccg atggtgaaga acgttggcaa
     3841 accattggaa aagtaaaagg cgtactaatg ctcttagtag ctcacaccat ctttgatgaa
     3901 gatgactgtg aaatcatacg aatcattagt gcaagacaag taactaaggc ggagcgagat
     3961 agatatgagc atggttagat actcacgcaa agaactgaat gaaaaattca gcgacaagca
     4021 agatgctgaa attcaacgct tgcttgctaa gggaacggtt cctgacgatc agctagatct
     4081 ctcagatatg cctgaaatta ccgactggag caatgctgta cgccataatc aattctatcg
     4141 cccagtgaag caacagactt ccattcgttt agatgctgat gtacttgcat ggtttaaagc
     4201 tcaaggcaaa ggctatcaaa cacgaatgaa tgaaattttg cgggatgcta tgctcaaaga
     4261 attaaaaaat catcagtaaa aatgggagct taagctccca tttttgactt cgcataaggt
     4321 gtattatgtt aattttaggg aaattaatca gttgatgaaa aatgaaaata ggaatttttt
     4381 ggtttttgca aaagcaagtt atcggcatag ctcatccatt caatttaaat gatgcagatt
     4441 caataggctt aattgatagt ccatatacac atgtcgatta ttggaaaaat atgcaaagtg
     4501 tatacccaga actacgtcat tatgaatatg aacagattcc aaggggtagg gtagttttcg
     4561 acgcaaataa agaaaaagca atcgtgtata tggacaaaaa attatttaac acagtaacag
     4621 ccactaaaat ttatgatttc tttgatatag attcagaaaa tgctattcca agaaaagatc
     4681 ctcactatcg tacttaattg tttaatttag gctaaaatga aattctcttt taataggggt
     4741 atgaagatga gaaagcgttt aaaagactat agccttgatc aaatttcaaa tcgtgaaaag
     4801 gtttcgggtc aagtattacc aatccaattt agcaatgacg aagcgactaa acgtgttgtg
     4861 atgcatagtg caaaacgagt cattaatcag cataaagctg aaatccaaga gcttgcttat
     4921 aaatgagttt gattagctca acattcgtca ttgctgtgca tgatgagatc ctattacaaa
     4981 ctggcgtagg gcgaataggc tgtcatattg aaaagctaga aagcgtatta agccgtatag
     5041 atcagcagat gtattacagt aatgtaagcg acatttttga gatcgcagca tggtttggta
     5101 tagctatctc aaaaggtcat gcttttgttg atggtaataa aagaactggt ttagctgtaa
     5161 tgctgactta tctcgaaatt caaggtgtta ccattcagga acaaacaggt ttagatgatt
     5221 tgatggtcga tattgttgaa tcacaagaag aacatgaatt attagccaaa attgtggctg
     5281 aattcttata cgagctttcg ataatttaaa ctaagaaatc agaatctgaa tatttggatc
     5341 atcgatattc gataaaagat tcttataaat taaataattt tcatcctctc ggcagataaa
     5401 aaatacttct gcaacatgat catattgagg caacatagag agtatagtgc caatcgcaat
     5461 ttcagcagcc ttttgaggtg gaaaaccata aacacctgta ctaatacatg gaaaagaaac
     5521 agtgagtgca tgaatttcat tagctttgaa tagtgcatta ctataagcat cacaaagcaa
     5581 ttgtggctca ttgtgttcgc catctaacca tcgtggtcca accgcatgaa taaggtattt
     5641 agcaggcaga ttacctgctg ttgttacttc tgcttgacct gtagggcatc cacctttttc
     5701 ttgatttaga cgaacacatt cttctttcat caatggtcca gcttttttat gaattacata
     5761 atcaagtccc ccaccaccaa gcaaagattt atttgctgag ttaacgatgg catgtacagc
     5821 aaaagctgtt atatctgctt ggatgagatg gacttttttc ataattagtt ttatagaata
     5881 cccttttgtt tcttatattc agtaatgatt gttcctaatt ctggttcaaa taatttgata
     5941 tattcatcag atagacctct actactagat ggatgtggta agcacacaac ttggcattgt
     6001 tcaaaattat caaagtaaac tttaaatcga gtaccatcaa aatctgtttt ttgtaccatt
     6061 tttaagggtt ctatttctgg accaacaata gcagtaaacc tatcccaaac atctatattt
     6121 ctcagaaaat taataagctt actacccata aataaaatta ctttgggtct caaaacttca
     6181 atatgatgaa taaaattatc aactgcttct ttttctaaga aacgattata gctttctata
     6241 tctgcattac tcgtataggc ccaattagtt tgaataatac atttatcaaa gtctgaacct
     6301 aaattttcat cgtttaatgg gtgtccccaa aaattaaacc attttttgat atttttatca
     6361 taaggccaag tattggaaga aggaccatac cttagggatt tattactaaa tgtacattca
     6421 atgctcatat ctactgtggg acggctatcg ctttcatcca gtttttgatc ttcttttgac
     6481 catccccatt catagccaca aatcattaat ccgtttttat cattaaagcc ttcgaaaatt
     6541 gaatttgcta aatttagatt tctttccatt attcttttct cagatgaact caaaccttaa
     6601 gttaacacat atattcaata taaatcaatt acttacttaa aaataggtac tattgtaatt
     6661 acattaacgg tttttaggag actctaggta tcatcacatt tgtaacaaaa aatataattt
     6721 atataaatca tatacataaa tattttattt tagatatgaa aattaatatt taacatgaaa
     6781 tttcttatac gaaatcaaaa atgggggcta taagctccca tttgattgta gtactctctg
     6841 atcaacaata gtccaaagaa tccagcttag tcttttctat ttagactgac tttctatacg
     6901 tgctttctca tacgcctgta ctttttctaa gatcggataa aaatcaaaac cttcatcctt
     6961 catcgttgat atgagcgata caatcacagc caaatgagag ggtacaaatc caactttatt
     7021 ataattcgta atcgagttag gtctcattcc aagtagttca gcaaattctt taattgaaag
     7081 acctgcttta ccgataagtc tatggaattc ttcgtaaggc atgaggttca aatttagcaa
     7141 atgataaagt gcaagtataa cataggctat tttttacata aaatgctaaa aaacacataa
     7201 aaatatgttt acaacacata tttttatgtg tttaattatt catatcaagc ctcttcttct
     7261 acatcggatt ttatatgaat tacttcaccc aacctcaatt atttatttca gcgaaagcaa
     7321 aaaaacttgg attacagttg attcaggaac tttatattca acgtagtacc cgcttggctt
     7381 tttatttaga tgtgaatcct tcttttgatg gacatgaagc tgttttattt tggactgaac
     7441 attatttagc tgaataccca gatgcaaatt tctcagatat tcagcagcaa tttatacagg
     7501 ctgtcccaca atcctaaaat ttttagtgat tgtgcttatt cccattgcag taacgcgact
     7561 aaaaatgcag aaacagggag cattcaaaat gctgtgtcag cccaataagc taaagctcaa
     7621 tcagaacatt caacgtccag ggcagatcgt tatagatgtt ttgctcatag acttaacagc
     7681 aacttattca ttatttttac gcagtacttt ctcaatttca atttgggtta aggtcattcc
     7741 ctcttttttc caggtatgca agccttcaag cacgtcaata aagagtttat tcctctcaaa
     7801 atattctttt ttatacttac tcacctcgga aaaaatttta gattttttga cctcatcatg
     7861 aaatacctta ttattcacaa ttcgattggt aatttgctca ggagtctctt tgctaatatc
     7921 aagattaagt ttcttaaatg cagctaaaac agcacctccg agttcaaact tccgtttatt
     7981 ttcagcttta cgtttttcac tagcattctt cttctgaatc tgacccaaat acagctctaa
     8041 gtcactatat tcaagctttt gtgctttaaa attcaaataa cgcgtacaca tcattttcag
     8101 aatattcatc tcaactggat ctagcccatc ctgtttataa ccttggtaca gctccatgaa
     8161 tttctcttca tactctgtgt gctgaaaatt ttgagaaagc tcatcaaaca acaactgaag
     8221 cttcaaatgt tcccgatatg gttttagcgc ttctttcacc aaaatctgct gagcttcaga
     8281 caacatttca aaattaaaat cttcagcttg tagtgcatca atgacctgat tttgaagttc
     8341 cgagcgagga tccttctcta atagaaactc aacgaaagca tgtaactctg ccatttgtaa
     8401 aagttgagaa ggtctaccac gcttttttgc tttttcctga gtcatatcaa ctaccgccaa
     8461 attttcagaa ctgctaccga ttcaaacatg gttttaccat atttgaatcg ggcagtctga
     8521 aaagaattat ttaaataaaa aataaaattt cttgaaaaga atttttattt tttatttaaa
     8581 taattcgcac ttgacagttt ctcttgatca gagaaactag tacgcaatgg ggcgattccc
     8641 ccttgacccc cttcaacctg atttttccag ccattgaggc attccaaaat caggacattt
     8701 ttaattccgt ttcactacat aaaaatgaaa atccgcagag aatgtaaaca tggccagcta
     8761 ccatttcagt gttaaaagca aaaataaagg ttatgccatg ggccattacc tctacattgc
     8821 ccgattgatg caatacgaaa acgtccgcaa aaaaagtgat gaacttttag aatccgttga
     8881 acttggcaaa tgcatgccgt cctgggtgaa agatccgatt gagttttggc aagccgctga
     8941 tacttatgag cgagcgaatg ccaaagtcta tatggagtat gaaattgccc tccccaacga
     9001 attcacgcca gaccagcgta aaacgctcat agagacgttt ttagacaaac atatagctcc
     9061 acagcaatat ccgcattcat acgccataca caacgttaaa tcgcgtctca gtggcgaaga
     9121 acagccccat tgccacctca tgttctccct caaagctgat gacggcattg aacgcacagc
     9181 cgaacaatac ttcaaacgct acaatcctca agatccggct aaaggcggtg ccaaaaaaat
     9241 ccagctgcag gatggccacg cagactattc cacattctta ctgcacatcc gcaaagcctg
     9301 ggaaaatcac ctcaatgatg ctttggccga gcactgccct actgtgacct accagatcaa
     9361 cggccaagat atcaccatca agaatcaagt atcagccgac aactatgaga aatacaatga
     9421 gatccacggc accttgtatc tcccggaacc caaattaggt gctggaaaac agaatgaaac
     9481 tgtggaatac ctggttgaga tccagaaaat tcggcagcac aatgccaacg agcgagaaat
     9541 ggaacaaaag cagcaggtac taaaacatga acctttctat tcatgcttga atgagcccta
     9601 taactcactc gaagtcatgt attaccttaa ccatttagcc aaaaaggcca aaaacaaaaa
     9661 agaactcgaa caagacttta acgaaattat taaaacacaa aagccaaaac ttcaggatga
     9721 gcactggaac ctgagcagaa tggataacca gcttcaaaac tcactttgtt acccagatcc
     9781 acaagttgat ttcaagccat taataggctt aaaactacag atggaagcga agccggttat
     9841 tcaagagaac aaagtatctg aaactattag accatcgaca caaccaaaac caaaaaataa
     9901 tgattatgat tttggtggtc cttaaaatta aggccttcct ctatacctca taggtatgta
     9961 aagagcgagt gtctacgagc gaactgaata catcaaaatt tcggctggtt ttttccttga
    10021 ttacaagctt cctatccatt aattacggtc agattagagc gtcccgaagg gcgcgaactg
    10081 cttgaaaatg cttctagacc gagcgaagcg agtaaaaaag ctttatgagc gtagcgaatt
    10141 ccgagttgct ttgctttttc ttaaagtcac aactggatga tccaaaaaat tgccccacga
    10201 atcgagcgaa agcgagattc aatagagttt gagcgtagcg aaaaccaagg gcaatttttc
    10261 atgcactggg cttttaatta tttttaaagt ttttaaatgc ttttagacat gctgtaagcc
    10321 tttatttata gtgtatacaa gggatataca acaccaaaaa gtcacctata caacaccaaa
    10381 aagtcaccta tacaacacca aaaagtcacc tatacaacac caaaaagtca cctatttaca
    10441 ccactgatta atgatggtgt aataacacca cgtataataa aggtgtagca a
//
