LOCUS       NZ_CP139273            11158 bp    DNA     circular CON 06-DEC-2023
DEFINITION  Acinetobacter pittii strain HCG62 plasmid pApiHCG62b, complete
            sequence.
ACCESSION   NZ_CP139273
VERSION     NZ_CP139273.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38315707
            Assembly: GCF_034071845.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S., Guerrero,G., Garza-Gonzalez,E.,
            Lozano-Zarain,P., Volkow,P., Morfin-Otero,Md.R. and Cevallos,M.A.
  TITLE     Acinetobacter pittii: intercontinental dissemination, pathogenetic
            potential, and antibiotic resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S. and Miguel,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2023) Programa de Genomica Evolutiva, Centro de
            Ciencias Genomicas, Av. Universidad s/n, Col Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: HCG62_genome
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1832x
            Sequencing Technology :: DNBseq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/06/2023 00:41:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,047
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,791
            CDSs (with protein)               :: 3,791
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 55 of 159
            Pseudo Genes (incomplete)         :: 95 of 159
            Pseudo Genes (internal stop)      :: 34 of 159
            Pseudo Genes (multiple problems)  :: 22 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139273.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="HCG62"
                     /isolation_source="tracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pApiHCG62b"
                     /geo_loc_name="Mexico: Jalisco, Guadalajara"
                     /lat_lon="20.6871 N 103.3438 W"
                     /collection_date="2018-11-29"
                     /collected_by="Hospital Civil de Guadalajara"
     gene            join(10949..11158,1..108)
                     /locus_tag="SOI80_RS20125"
                     /old_locus_tag="SOI80_20130"
     CDS             join(10949..11158,1..108)
                     /locus_tag="SOI80_RS20125"
                     /old_locus_tag="SOI80_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000900860.1"
                     /translation="MLGKNHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            629..973
                     /locus_tag="SOI80_RS20130"
                     /old_locus_tag="SOI80_20135"
     CDS             629..973
                     /locus_tag="SOI80_RS20130"
                     /old_locus_tag="SOI80_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_005065055.1"
                     /translation="MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSGKKRFYTEMLAIADKQ
                     YELHLSTLGE"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            978..1280
                     /locus_tag="SOI80_RS20135"
                     /old_locus_tag="SOI80_20140"
     CDS             978..1280
                     /locus_tag="SOI80_RS20135"
                     /old_locus_tag="SOI80_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_005065057.1"
                     /translation="MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            1372..1773
                     /locus_tag="SOI80_RS20140"
                     /old_locus_tag="SOI80_20145"
     CDS             1372..1773
                     /locus_tag="SOI80_RS20140"
                     /old_locus_tag="SOI80_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065059.1"
                     /translation="MTDKKQNDHLNLDGINSSYNDGDGLRINNPEDFRSITISNGYFSN
                     NKGNGITIGSPQQSPLEIILTQLAPKLPDTIQPYELASVIQNLLESTNQEEISQKLMTS
                     GLKEKFKDPNLWISFSSLLFSLIFQFSSK"
                     /name="hypothetical protein"
     gene            1825..2499
                     /locus_tag="SOI80_RS20145"
                     /old_locus_tag="SOI80_20150"
     CDS             1825..2499
                     /locus_tag="SOI80_RS20145"
                     /old_locus_tag="SOI80_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_005065062.1"
                     /translation="MSQKILQRYEQLLTDSASIKAMTKKAYSEYSGSYDTLGDEGDALY
                     LEWKVKVKNLLLLSCGEHSIHYRDFLDAEETQSFDTNTRIISRLIPILKASYDDFKNGF
                     LTSFKQIVQAEVFDSELEQARSLLSSGYKNSAAVIAGVVLETAIKELCLNNGIELEKKK
                     LTRLNEDLAKAGVYNPLQQKLITAMADIRNNAAHGDYDQFTKEDVHRMIEDIERFLLAY
                     SS"
                     /name="HEPN domain-containing protein"
     gene            complement(2590..2901)
                     /locus_tag="SOI80_RS20150"
                     /old_locus_tag="SOI80_20155"
     CDS             complement(2590..2901)
                     /locus_tag="SOI80_RS20150"
                     /old_locus_tag="SOI80_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005065064.1"
                     /translation="MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEI
                     TDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQGKGYQTRMNKILREAMLEDLKNH"
                     /name="BrnA antitoxin family protein"
     gene            complement(2888..3175)
                     /locus_tag="SOI80_RS20155"
                     /old_locus_tag="SOI80_20160"
     CDS             complement(2888..3175)
                     /locus_tag="SOI80_RS20155"
                     /old_locus_tag="SOI80_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002124950.1"
                     /translation="MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIISARQVTKAERNQYEHG"
                     /name="BrnT family toxin"
     gene            3688..5175
                     /locus_tag="SOI80_RS20160"
                     /old_locus_tag="SOI80_20165"
     CDS             3688..5175
                     /locus_tag="SOI80_RS20160"
                     /old_locus_tag="SOI80_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953322.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005065066.1"
                     /translation="MLSTIREQWFSNIRGDVLAGIVVALALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVIAFVGGRPGMISGATGAMALLMVTLVKEHGLQYLLAATILTGFIQILAG
                     YLKLGALMRFVSKSVVIGFVNALAILIFMAQLPELTNVTWHVYAMTIGGLAIIYLFPYI
                     PVIGKLLPSPLICIVLLTLFALFIGLDVRTVGDMGQLPDTLPIFLLPDIPLNLETLTII
                     LPYSLGLAAVGLLESMMTATIVDDLTDTNSDKNRECKGQGVANIASGFLGGMAGCAMIG
                     QSIINVKSGGGTRLSTFSAGVFLLIMVVFISDWLKAIPMAALVAVMIMVAIGTFNWDSL
                     RNIRQYPLSSNIVMIVTVIVVVATHNLAYGVLVGVLLSALFFANKIERYMAIQSEFNEP
                     ENTRTYTVTGQVFFSSADKFTSAFDFKEALSKVVIDVSQAHFWDVSAVAAVDKVVIKFR
                     REGTEVELIGMNEASKTLVDKFGIHDKPDALDRLNSH"
                     /name="SulP family inorganic anion transporter"
     gene            5188..6039
                     /locus_tag="SOI80_RS20165"
                     /old_locus_tag="SOI80_20170"
     CDS             5188..6039
                     /locus_tag="SOI80_RS20165"
                     /old_locus_tag="SOI80_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034565.1"
                     /GO_function="GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_005065068.1"
                     /translation="MSKIIACIDGSLATNTVCDYAAWFSDKLSSPLKLLHVIDKPKAKA
                     PQDLSGAIGLGSRETLLKELVEIEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSLLETIMGMEEDIRVLVMGKHGAETEHDSSKVGTHIENVVRALHKPVLITSSPFSPP
                     KSFLIAFDGSPTARICVDKIANSPLLKTLTAHVVYVGKPNSDMTLQVTWAKELLANNGF
                     DVIDSMLDGDVEKSILDYQEKNVIDMIVIGAYGHSKIRQFFIGSTTTKLLSSSTKPVLL
                     LR"
                     /name="universal stress protein"
     gene            complement(6271..6801)
                     /locus_tag="SOI80_RS20170"
                     /old_locus_tag="SOI80_20175"
     CDS             complement(6271..6801)
                     /locus_tag="SOI80_RS20170"
                     /old_locus_tag="SOI80_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005065050.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSQNKIDLAEAIRV
                     FGEPVKKQDVKEPVKIDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPNMTTFTDQELVQPSIAKPDPEPDTAEAKHDGLTTPERRENKWIPVPEHIEPEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6880..7809)
                     /gene="repM"
                     /locus_tag="SOI80_RS20175"
                     /old_locus_tag="SOI80_20180"
     CDS             complement(6880..7809)
                     /gene="repM"
                     /locus_tag="SOI80_RS20175"
                     /old_locus_tag="SOI80_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005065049.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGFAPA"
                     /name="repM"
     gene            complement(8507..9220)
                     /locus_tag="SOI80_RS20180"
                     /old_locus_tag="SOI80_20185"
     CDS             complement(8507..9220)
                     /locus_tag="SOI80_RS20180"
                     /old_locus_tag="SOI80_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065071.1"
                     /translation="MAGRKTFALTQQEIIDLDKHIRALPFKRRMQDAFLAMISERESMK
                     QLNEEDLKLIQRCRYERNTYNKRIVQLQLIQKTEPQKLKKLELNILELSKKTDIDAYFA
                     MHDALNEVLKIKRNQTSEKNITAKVERVLNPEKYNQAPLSKSLRKQQDHIKYFIGSLYL
                     NLLEQSGLERSNSNEDLDRMLDLFKQAQSGAEKNNAFSLDQNRKTDILSAKSLYEMEKL
                     IKQAKEDPRNPFNKA"
                     /name="hypothetical protein"
     gene            9676..10560
                     /locus_tag="SOI80_RS20185"
                     /old_locus_tag="SOI80_20190"
     CDS             9676..10560
                     /locus_tag="SOI80_RS20185"
                     /old_locus_tag="SOI80_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_005065070.1"
                     /translation="MFYLNLKHNIKKEQSSLNAFHYLTRTAHFENQKDNEKLEFMRYGN
                     MPKWAENKPKLFWKSADQFEISRGRTSSTLTIALPKELILEQRAELVQKLIDQFAGQYQ
                     FPYTAVIHNHPSEITGEDQPHLHLMYSERTISDDIERPPEQFFKQYRPKNPTQGGAQKL
                     TADALGFGRNQIKVFREKTQELMNLFLEKYAPTKKVMVYGVEIEVKNQVSCLSNDDFNQ
                     KFGTKLQDVPQIPRWKLYSADPIIQLDVEAQKNTIKKIRNQNNAELYGKEYDLEISRRH
                     VLTQKKDPGFSLD"
                     /name="MobA/MobL family protein"
     Typing          complement(6894..7809)
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.884"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          9677..10557
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaactaaaag atgaagttat gcatttaaaa aaacaactta acttttttaa atatacttca
       61 atctcaaaca aaaatagact attaatttct cataaacatt ccatttaaca taatagagct
      121 tatacgaaat gctttttaaa atctccttgt ttttcatata gttaaaaatt tttcatcccc
      181 ctaaaaaacc acaaaaaacc gactgggaaa caagtcggtt tctttttaag caaaactgta
      241 acttttagcc aataaaatcg atcaaaaaac attcgttttt cattttttcc tgatttctca
      301 atggtcacac ttcacaaata atccccaatt tctgcggaca actcttggga taaaataggc
      361 caactttgta agctaaagct tacagagatt taaggatttt gctgattggt aatccaaggt
      421 actagcccta ttatgaatac ataagggaac tggatgtctc cagacaacaa gaataagaaa
      481 atagcgcaag gaatgggcag cacgagatca gtaagacgag cacccgatat gaaaaagccc
      541 gacaggatgt cgggcttttt tattgccatt tgcaaagtta ttcaatcagc tttaatatat
      601 agactataat ctatattatg gttgtaatat gtggacagtc attacaacgg atctgtttaa
      661 tgaatggctt gaacagcaag atgaaataac acaagagaaa gtcttagctg cgttggttgt
      721 tctgcaactg caaggaccaa gtttaggtcg tcccttagta gataccgtct acgattctaa
      781 atttaccaat atgaaggaac tacgtgttca gcatcgaggt aaaccattaa gagcattttt
      841 tgcattcgat ccattacgac aagcaattgt actgtgtatt ggtgataaga gcggtaaaaa
      901 gcgtttttat acagagatgc tagcaattgc agataagcaa tatgaacttc atctctcaac
      961 attaggagaa taatctgatg gctaagaccc tacaagaatt attggctaat cgctctcctg
     1021 agagccaagc ccgtattcaa aaaatggctg atgaattgct attagaaaat cagctttacc
     1081 ttattcgtga agaactcgaa atctctcaaa aagaactggc tgaaactttg ggaataaaac
     1141 agccatcgct ttcagcaata gaaaatcgtg gcaatgatct taagatttca accatgaaaa
     1201 agtatgttga agctatggga ggaaagctcc gtattgatgt ggaacttcca actgggaaac
     1261 atataggatt caatgtatag atatagaaaa gtgagtttaa gctcactttt ttcatttggt
     1321 ctgatcagta tgatataagt tataaatcat tcaaaagata aaggcttata tatgactgat
     1381 aaaaaacaga acgatcatct aaatcttgat ggaatcaact catcttataa tgatggtgat
     1441 gggctaagaa taaataatcc tgaggatttc agaagtatta ccatatctaa tggctatttc
     1501 tcaaacaata aagggaatgg aattactatt ggctcaccgc aacaatcacc attagaaatc
     1561 attcttactc aattagcccc aaaacttcca gatactattc agccttatga attggcatca
     1621 gttattcaaa accttctgga atcaactaat caagaagaaa tatctcaaaa attaatgact
     1681 tcaggcctta aagaaaaatt taaagatcct aatctttgga tatctttcag tagtttattg
     1741 ttttcattaa ttttccaatt ttcatctaaa tagatttata gagttcaatt acttaatttg
     1801 gaaaccctaa aaaactgagt aaacatgagt caaaaaatat tacagcgtta tgaacaacta
     1861 ctaaccgatt ctgcttccat caaagctatg acaaaaaaag cttactcaga atatagcggt
     1921 agctatgata ctttgggtga tgagggggat gctctttatc ttgaatggaa agttaaagtt
     1981 aaaaatctat tattactatc atgtggtgaa cattctattc attaccgtga ttttttagat
     2041 gctgaagaaa cacagagctt tgatactaat actagaataa tcagtaggtt aattccaatt
     2101 ttaaaagcat cgtatgatga ttttaaaaat ggttttttaa cttctttcaa gcaaattgtt
     2161 caagctgaag tttttgatag tgaattagaa caagcacgat cgctgttaag tagtggttat
     2221 aaaaattcag ctgctgtaat tgctggtgta gttctagaaa ccgcaattaa agaactatgt
     2281 ttgaataatg gaatagaatt agagaagaaa aaattaacac gattgaatga agacttggca
     2341 aaagcgggag tttataaccc tctacagcaa aaactaatta cagctatggc tgatattcga
     2401 aataatgcgg cccatggaga ttatgatcag tttacaaaag aagatgttca caggatgatc
     2461 gaagatatcg aaagatttct actagcttac tcttcgtaag actgtcagat ttacatagaa
     2521 aaaatttaat tatttaacat aaaatctctt atgcgaagca aaaatgggag tctatagctc
     2581 ccattttcat caatgattct taagatcttc tagcattgct tcacgcagaa ttttattcat
     2641 gcgtgtctga tagcctttgc cttgagcctt taaccatgca agtacatctg catctagacg
     2701 aacagatgtt tgttgcttca ctggacgata aaattgccca tggcgaacag catgactcca
     2761 gtcggtaatc tcaggaatgt cagatagatc taattgttca tctggtacag ttccttttgc
     2821 aagcaagcgt ttaatttcag catcttgctt gtcactgaat ttttcattca gttctttgcg
     2881 tgtatatcta accatgctca tattgattgc gttccgcttt agtgacttgc cttgcactaa
     2941 tgattcgtat gatctcacag tcatcatcgt caatgattgt atgagccacc aataacatca
     3001 gtacaccttt aaccataccg atcgtttgcc aacgttcttc accattggta tgtcgatcct
     3061 ggattgagat ccgtaaagga tcttcaaaaa cgaggcttgc cgtttcaaaa gatacatcat
     3121 gttttttctg attcttttgg tttttagcct catcccattc gaaatacagt tccataaaaa
     3181 acattcaaat ttgtatatac aataatgtat cacaaatttg tatcactcaa gctaattgga
     3241 gtaattttta gtcaaaatta ttggcaagaa gtatcaaact tgctcaaaaa aaatccgctt
     3301 tgtttccaaa gcggattttt gcggtggtta cgggtgatgt aaaatagtta agcatatgat
     3361 tttatttaac ttataaaaga cgatttcgga taacgcccat tatgttaaat gggcataaat
     3421 caattctaaa attatctatg ataattctta gcttttactt cagtactgcg tttagggtgg
     3481 atagaaaagg ggctagctgc tatgattgcc cccgaaatcc tccaaaatag atatctttct
     3541 taatatgaca cttacgccac cataagttta tttacctctt aaatcagtaa ataactcatc
     3601 actgtctagt gatgtggtgt agtagcttgt gtgtcgtata tatcacatca ctagcctttc
     3661 ttaaaattta aaaaaatagg ctatttcatg ttatccacta ttcgagaaca atggttctct
     3721 aatattcgcg gggatgtcct tgcgggtatt gttgtcgctt tggctctaat tcctgaagca
     3781 attgccttct ctattattgc aggtgtcgat cctaaagtcg ggttatacgc ttcattctgt
     3841 attgccgtgg tgattgcttt cgtaggtggt cgccctggta tgatttctgg ggcaaccggt
     3901 gccatggcgt tattaatggt aacactggtt aaagagcatg gtctgcaata cttattagca
     3961 gccacaattt tgacaggatt tattcaaatc ttagccggct atctaaaatt aggtgccttg
     4021 atgcggtttg tatccaagtc agttgtgatt ggctttgtga atgccctggc tattctaatt
     4081 tttatggctc aattacctga actcactaat gtgacatggc atgtctatgc gatgacaatt
     4141 ggtggtttag ccattattta tctattccct tatattccag tgatagggaa gcttttaccc
     4201 tctcctctca tctgtattgt cttacttacg ctctttgcct tattcattgg tttagatgta
     4261 agaaccgtcg gagatatggg gcaattaccg gatacgcttc caattttctt gcttcctgat
     4321 attcctttaa atcttgaaac cttaacgatt attttgcctt attcactggg attagcagcc
     4381 gtaggtttgc ttgaatctat gatgacagca acgattgtcg atgatttaac agataccaat
     4441 agtgataaaa accgtgagtg taaaggacaa ggcgttgcca atattgcttc aggtttctta
     4501 ggcggaatgg caggctgtgc catgattggt caatcgatta ttaatgtgaa atctggtggg
     4561 ggtacccgtt tatctacatt tagtgcaggg gtctttctgt taattatggt ggtgtttatc
     4621 agtgactggc ttaaagccat tccaatggct gcactggttg ctgtcatgat catggtagca
     4681 attggaacat ttaactggga ttcactacgc aatatccgcc aatatccctt atctagcaat
     4741 attgtgatga tcgttacagt catcgtggtc gttgcgacac ataatttggc ttatggcgta
     4801 ttagttggtg tacttctttc tgcattattt tttgccaaca aaattgaacg ctacatggca
     4861 attcagtccg aatttaatga acctgaaaat acccgtactt atacggtaac aggtcaagtg
     4921 ttcttcagct cagcagataa atttacttct gcatttgatt tcaaagaagc actttcaaaa
     4981 gttgtaattg atgttagtca ggctcacttt tgggatgttt ctgcggtcgc tgccgtagat
     5041 aaagtggtca ttaagtttag acgtgaagga acagaagtcg aacttattgg aatgaatgaa
     5101 gccagcaaaa ccttagtcga taaatttggc atccatgata aaccagatgc attagatcgc
     5161 ttaaatagcc actaagaggg atgaaaaatg tcaaaaatta ttgcatgtat tgatggatca
     5221 ttagcaacaa ataccgtttg tgattatgca gcttggttta gcgataaact tagttcaccg
     5281 cttaagttat tacatgttat tgataagcca aaagcgaaag caccacaaga tttaagcggt
     5341 gcaatcggct tgggaagtcg agaaaccttg ctcaaagaat tggttgaaat tgaagagcgt
     5401 aaggggaaga ttgaactaga gcatggccaa attcttcttc gggaagcaaa gaattatcta
     5461 ttggagaagt tctctattga tgctcaaagt tttcaacgcc acggcagtct tttagagaca
     5521 atcatgggaa tggaagagga tattcgtgtt cttgttatgg gtaaacatgg ggctgaaacc
     5581 gagcatgatt ccagtaaagt tggaacccat attgaaaatg ttgttcgtgc acttcataaa
     5641 cccgtattaa taacatcttc accctttagt ccacctaagt cttttttaat tgcttttgat
     5701 ggaagtccta ctgcacgtat ttgtgtagac aaaattgcaa atagcccttt attaaaaacc
     5761 ctgacagctc atgtggtata tgttggtaag ccaaatagtg atatgacatt acaagtcact
     5821 tgggcaaaag agctacttgc aaataatggt tttgatgtaa ttgatagcat gcttgacggt
     5881 gatgttgaga aatctatatt ggattatcaa gaaaaaaacg ttattgatat gattgtaatt
     5941 ggtgcatatg gtcattcaaa gatacgccag ttttttattg gtagtacgac gaccaaattg
     6001 ttatcaagct ctacaaaacc agtattatta ttaagataat ttttaaaaga taaaaggata
     6061 gtttttaact atcccttttt tagattttct aaaattaaca taatacacct tatacgaaat
     6121 gtgttataat tttccttaga aatcaacgct gtagtttctt ttttgcccaa cctccccaag
     6181 gtgggctttt tttttatgat ttttcacgtt ccacgcttat tatttaatca aagtttcacg
     6241 actttattca tattaattaa acaatcgtgg ttaatcatag ggtctaaaaa aacggctcca
     6301 aaagcctctt ttctcaggct ctggttcaat gtgctctggc actggaatcc acttattttc
     6361 cctccgctct ggagtagtca atccgtcatg tttagcttct gctgtgtcag gttcgggatc
     6421 tggtttggct atgctgggtt gcactaattc ttgatcggta aacgtagtca tattaggttt
     6481 tggggcttct aataggcgtt gcatcgtttc aatttggttc tgatagaaag attcccgatc
     6541 tttggcatca tcaagctggt ttttcagcat gtctatctgc tgtctaagta gtaaaacttc
     6601 tgccgaattt ggactgtcta tttttacagg ttcttttaca tcctgttttt tgacaggttc
     6661 cccgaaaaca cggatagcct cggctagatc gatcttattc tgggaatttt tacttaaaat
     6721 tcctttcttt atgttgttgt aaatcgtttg tctattgata ttgtaaagct tagaaagctc
     6781 gagaactgta aggcttttca tgttgtaaat ctatgcctaa acctgtcaaa agttgttgtc
     6841 tattagactg tctaataaac aaatgatcaa gtttaaattt taggcaggtg caaagccaac
     6901 tttttgaaga taagggaata attcttggaa tttttgataa tcttggagca tttctgcaat
     6961 gcgtatagca aattgttgga agctttcggt gccttctgaa tattgcccca tctcaggtaa
     7021 ttcagaaagt ttgttggcaa agagatgacg ttgtttatca ctcattttgg tgaaaagggc
     7081 taatgtatcc gttcccttaa ttactttgtc agagttggtt tttttctgct taaaactgaa
     7141 agaaaaaccg gaaattgaac gtcctttttt atgttgttca tatttagccg ttatatcggt
     7201 taactcattt acttgcttga gggcaaaatc taaaacatac gttttaaaat tactcattgt
     7261 tttgtattga tgagcttcga caccaagttg ttgtctaaag atcgatagat caaaaattgg
     7321 cgttttacca gcagatctcc attgaattaa taactcgtat aaccgaatag cataagagct
     7381 agttaatctc gaaacctgct ccaattcata tttagtaaag ttttcttcca gtcgagttat
     7441 gagtggaaca atgtcttgcg tgaaacgtag gaaaacgatc ccggattgag gttcatatcc
     7501 aattttatcg acccaacgac agtgaaagct acgatcctta ccagtcttag gattaatgtc
     7561 atgatacgtg acatagcgat caaacaagct tttagatgca tcccgaagca cggtataagc
     7621 agtgtgtttc tcgacattaa aggtattgat ataactactt gcatgtactt ctaaaaggct
     7681 attttcagtt atcccgtgcc ctgtttcccg agcttcagca atggctaata ggatcagcct
     7741 ttgttcaact gtatctaggg tatagctagc ttgaattaaa gcattatcct ttacgattaa
     7801 ttcactcatt tctaataata aatttataat taaactaggt tgtacaatag actattttaa
     7861 ttgaattgta aatcgactta atttggggtc gataaaccta gttttaaggt cgataaacct
     7921 agttttatgg gtcgataaac ctagttttat gggtcgataa acctagtttt atgggtcgat
     7981 aaacctagtt ttattaactc taaaactatt atgaataaag ggttacagct tacttaaaag
     8041 catttaaaag attaaaaata attaaaagcc caggtaaaga aaaattgccc ttggttttcg
     8101 cttcgctcaa actctattga acttcgcttt cgctcagttc gtcggggcaa ttttttggtg
     8161 agcacttacg tgactttaaa aaagcaaaag caactcggaa ttcgctacgc tcataaaact
     8221 tttttactcg ctacgctcga tctagactca aatttctaca acttttccat gcttatttag
     8281 gtttcacagc ctaaaaagcg gtttttcagg gagtctaggc aaaattttcc aaggatcgct
     8341 cgtagacact cgctttgttc ttatcggtaa aatcaaatga tctccaaaat actctaattt
     8401 gcgtttagag cactctaatt tttttattga tggggaatat aggtttaggc taaaaaaggc
     8461 atatacggct tcaacagtgt atttaaaccc tattatttga gtcaatctaa gctttattga
     8521 aaggattcct tggatcttct ttggcttgct taatcaattt ctccatttca tatagcgatt
     8581 tagccgataa aatatctgtt tttctatttt gatccaggct aaatgcattg ttcttttctg
     8641 ctccggattg tgcctgctta aataaatcga gcattcgatc taaatcttcg tttgaatttg
     8701 atcgctctaa accgctttgt tccaataagt ttaaatatag tgatccgata aaatatttaa
     8761 tgtggtcttg ctgttttctt aaagatttac taaggggggc ctggttatat ttctcagggt
     8821 ttaaaactcg ttcgaccttc gctgtaatat tcttttcaga agtctgattt cttttaattt
     8881 ttaaaacttc atttaatgca tcatgcattg caaagtaagc atcaatatct gtttttttac
     8941 ttaattctag aatattcaat tctaattttt ttaatttctg tggctcggtt ttttggatca
     9001 gttgtaattg aactatacgt ttattataag tattgcgctc ataacgacat cgttgaataa
     9061 gttttaaatc ttcttcattc agttgtttca ttgattctcg ttcagaaatc attgctaaaa
     9121 aagcatcttg cattcgtctt ttaaacggca gcgctcgaat atgtttatcc aaatcgatga
     9181 tttcttgttg tgttaaggca aatgtttttc taccggccaa agcggatttc ctgattttgg
     9241 gtatctaaat tgatgttagc ggaattctga tggtgttgaa acaatatttt atgttataca
     9301 taaaatattg tttatctcgt ttccgtgtct ttttttaaca atgattgctt tagcaatcat
     9361 tgttaaaaaa agacacttct actcgctcct gcggaggcta cataaaccac tgatttaatt
     9421 attaaattat gagtttttct tcgggtttta acggtatttt tcttaatggt gaaaaagtaa
     9481 gcaatgtctg ccgttacaag aaatgggaaa ggggtgcaac aaggaaaaca gcgaatttta
     9541 gacgagtgca acaaactttt ttgatgaaat atcgatgttt gcaacaagaa gagactgctc
     9601 tctgcaacaa gaattagccc cttgttacaa gaaaatttgc tgtttcgtta cacgtaattt
     9661 gtttgaggtg aactgatgtt ttatttaaat ctaaagcata atattaaaaa agagcagagt
     9721 tctctgaatg cattccatta tttaactcgg acggcacatt ttgaaaatca aaaagacaat
     9781 gaaaaattag agtttatgcg gtatggaaat atgcccaaat gggcagaaaa taaaccaaaa
     9841 cttttttgga aaagtgctga tcaatttgaa atttcccggg gaagaacaag ctctacttta
     9901 accattgctt taccaaaaga gttaatacta gaacaacgag cagaattggt tcagaagtta
     9961 atagatcagt ttgccggaca atatcaattt ccttataccg cagtcattca taatcatccc
    10021 agtgaaatta ctggagagga tcaaccacat ttacatttga tgtattcaga aagaacaatt
    10081 tcagatgata ttgaacgacc accagagcag ttttttaaac agtaccggcc aaaaaatcca
    10141 actcaaggtg gtgcgcaaaa gttaacagct gatgcattag gttttgggcg aaatcaaatc
    10201 aaagtattcc gtgaaaaaac tcaagaatta atgaatttgt ttttagaaaa atatgcacca
    10261 actaaaaaag taatggttta tggtgtagaa attgaagtga aaaatcaggt cagttgccta
    10321 tctaatgacg atttcaatca aaaattcggg accaaattac aggatgtacc acaaatccct
    10381 cgttggaaac tttatagcgc tgatccaatt attcagcttg atgttgaagc tcaaaaaaac
    10441 acaattaaaa aaattagaaa tcagaacaat gctgaacttt atggcaagga atatgattta
    10501 gaaatatcta gacgccatgt gcttactcag aaaaaagatc ctggctttag cttagattga
    10561 tttttataag tggaaaattt gtgttttttg aacatggaat cttgaaaaaa cttatccata
    10621 ttgtgagtga tttttagcca caacatggaa aatttgtgtt ttttaggctg caatgatgga
    10681 tagattgtgt ttttgattca ggtcaataag gaaaacctag atttttgccc ataaatgtaa
    10741 aatctaggtt tttcgcataa ggtgtattat gttaataata taaattttta aaagcatatt
    10801 gatttaataa tagaaaaatt atcagagtag ggcatgtgta aagtaaacgt tcgattatta
    10861 accaataatg attaaagatt tatatcttgt atgtgttaat tttattaaca tatatatacg
    10921 aaattaataa tgccttaagt gaaatagtat gctaggtaaa aatcatcatt ttttaaaaaa
    10981 attttcgatc agtgatgctg ctatagcgta taaaaaacct cgctcgacta tacttaaata
    11041 tataaaaagc ggagatttcg aacttgacta caataaattg attgaataca gtcagttagt
    11101 tagaaaattt ggtgaactta cttaccctat acaggacgaa agagagcaac aaatcaaa
//
