LOCUS       NZ_CP139261             9973 bp    DNA     circular CON 06-DEC-2023
DEFINITION  Acinetobacter pittii strain HUM3 plasmid pApiHUM3a, complete
            sequence.
ACCESSION   NZ_CP139261
VERSION     NZ_CP139261.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38315710
            Assembly: GCF_034066905.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S., Guerrero,G., Garza-Gonzalez,E.,
            Lozano-Zarain,P., Volkow,P., Morfin-Otero,Md.R. and Cevallos,M.A.
  TITLE     Acinetobacter pittii: intercontinental dissemination, pathogenetic
            potential, and antibiotic resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9973)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S. and Miguel,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2023) Programa de Genomica Evolutiva, Centro de
            Ciencias Genomicas, Av. Universidad s/n, Col Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: HUM3_genome
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 993x
            Sequencing Technology :: DNBseq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/06/2023 00:00:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,813
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,632
            CDSs (with protein)               :: 3,632
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 35 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 17 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139261.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="HUM3"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pApiHUM3a"
                     /geo_loc_name="Mexico: NuevoLeon, Monterey"
                     /lat_lon="25.6888 N 100.3502 W"
                     /collection_date="2021-06-17"
                     /collected_by="Hospital Universitario Dr. Jose Eleuterio
                     Gonzalez"
     gene            1..936
                     /gene="repM"
                     /locus_tag="SOI70_RS19010"
                     /old_locus_tag="SOI70_19035"
     CDS             1..936
                     /gene="repM"
                     /locus_tag="SOI70_RS19010"
                     /old_locus_tag="SOI70_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237873.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_199982916.1"
                     /translation="MPTSELIVKDNALINASYNLDTVEQRLILLAILQARETKTGIDAN
                     TRLRIHASDYMSRFNVNKHAAYKALKTAVTNLFGRQFSYQTIDEKTGKSKKVISRWVQN
                     ISYIDDAATLEVTFTMDVVPLITRLEKQFTSYQLKQVTQLTGKYAIRLYELLIAWREVG
                     KTPIFEVSDFRSKLGLTSDDYPRLDTFKRRVLESAVKQINEHTDIIVKVEQHKEGRSIS
                     GFSFSFKQKRNPNKVIERKKDPNTLDIFSRITDAQRHLFANKLSELPEMGKYSQGTESY
                     QQFAIRIAEMLQDPAKFQELHPYLEKVGYR"
                     /name="repM"
     gene            1005..1550
                     /locus_tag="SOI70_RS19015"
                     /old_locus_tag="SOI70_19040"
     CDS             1005..1550
                     /locus_tag="SOI70_RS19015"
                     /old_locus_tag="SOI70_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_171073080.1"
                     /translation="MKALSVIELSQLYGMNRQSIYKRINKGDLSKNSDGKIDLSEAIRV
                     FGEPSNKNNNVTTLQSTTVQKDTEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKTNMTTFTDPKPSQDIATDPRSEPEPKHDGLTTPEQRENKRIPVPEQV
                     EQEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1860..2426)
                     /locus_tag="SOI70_RS19020"
                     /old_locus_tag="SOI70_19045"
     CDS             complement(1860..2426)
                     /locus_tag="SOI70_RS19020"
                     /old_locus_tag="SOI70_19045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000156164.1"
                     /translation="MTISTYNQILELANKYIQKVGFNAFSYSDLANEIGIKKASIHYHF
                     PSKTDLGIAYCHFKLNQLLELETRINSHASIEAKLFEYRNFFIQCAEKKEMCGIHSMLT
                     ETDSFSETLQSAVFQLVESELRIIEHIFKNDLDENISMITKVSNYELALMVNSTIKGAL
                     LMNRLSKKNIYEHSLNALIQLILSK"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            complement(2436..2867)
                     /locus_tag="SOI70_RS19025"
                     /old_locus_tag="SOI70_19050"
     CDS             complement(2436..2867)
                     /locus_tag="SOI70_RS19025"
                     /old_locus_tag="SOI70_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="WP_000517302.1"
                     /translation="MGKLLSNFINKQADILVLKLSVIFIFVLFGTYKWFDFEVVALEPI
                     LSKSWLSILYTLFGHYGASYFLGVVENITFIALIIGFFKPKIGLIGDLLVILTGITTLS
                     IIPQLGHIDSFIIKDVLLVGAGLVLLKFDLKLIYHNKLL"
                     /name="DUF417 family protein"
     gene            3296..3790
                     /locus_tag="SOI70_RS19030"
                     /old_locus_tag="SOI70_19055"
     CDS             3296..3790
                     /locus_tag="SOI70_RS19030"
                     /old_locus_tag="SOI70_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="WP_000483698.1"
                     /translation="MFNYLKEKNFIIAEKNKGEFNFISLLRWSLVIIFIWFGGMKFTHY
                     EAIGIAPFIENSPIVSWLHNLFGIQGASYFLGCMELLTAFILLLGAFKPILSALGGLMS
                     AITFLITLTFMLTTPGVIEPSAGGFPALSGAPGQFLLKDLVLLAASLCILKASINRRNP
                     Y"
                     /name="DUF417 family protein"
     gene            3847..4287
                     /gene="msrB"
                     /locus_tag="SOI70_RS19035"
                     /old_locus_tag="SOI70_19060"
     CDS             3847..4287
                     /gene="msrB"
                     /locus_tag="SOI70_RS19035"
                     /old_locus_tag="SOI70_19060"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944401.1"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA];
                     GO:0006979 - response to oxidative stress [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_000221341.1"
                     /translation="MTYKKTIESIDKLNVDQYRVTQNNGTERPFEGEYDKHFLPGIYVD
                     IVSGEPLFSSTKKYNSGCGWPAFSKPIENVTEHADFSHGMRRVEVRSKHANSHLGHVFT
                     DGPQSDGGLRYCINSAALRFIPLLEMERQGYADYIKYVEVNT"
                     /name="msrB"
     gene            4287..4772
                     /gene="msrA"
                     /locus_tag="SOI70_RS19040"
                     /old_locus_tag="SOI70_19065"
     CDS             4287..4772
                     /gene="msrA"
                     /locus_tag="SOI70_RS19040"
                     /old_locus_tag="SOI70_19065"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332042.1"
                     /GO_function="GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA]; GO:0008113 - peptide-methionine
                     (S)-S-oxide reductase activity [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA];
                     GO:0006979 - response to oxidative stress [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="WP_000438903.1"
                     /translation="MERAILAGGCFWGLQDLIRKLPGVEHTRVGYTSGDIANATYRNHG
                     THAEAIEIIFHSNIISYRKLLEFFFQIHDPSTLNRQGNDIGTSYRSGIYYTTEEQHQIA
                     LKTIQDVNSSGIWPGKVRTEVKAASDFWEAEPEHQDYLERYPTGYTCHRVRPDWVLP"
                     /name="msrA"
     gene            4891..6056
                     /locus_tag="SOI70_RS19045"
                     /old_locus_tag="SOI70_19070"
     CDS             join(4891..5149,5149..6056)
                     /locus_tag="SOI70_RS19045"
                     /old_locus_tag="SOI70_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611942.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISAba22 family transposase"
                     /protein_id="WP_320432714.1"
                     /translation="MSKKHKTYTTEFKAEAIKLIEANQGNVSETARQLSISMQTLSNWN
                     TKAKAGTLAGTKQYSPDLNALLEENKKLKQQLKIAEMEREFFKKGSSVLCQRKSVRYAY
                     MKQKRYSFPITLMARLLHVSVSCFYDWLKKGVSKRTIQRNQQTILVKIAHEETRQSYGY
                     IRLTKYLQAQGIKMSMYAVRQIKALNHLYCKRHKRFKRTTNSDHNRAIYENLLEQQFSM
                     TRPNQAWSSDITYIWTVEGWLYLAAVKDLYTKQVVGYSLNERMTTQLVCNALNMAIHNQ
                     KPTKELIVHSDRGSQYCSHEYRNILEQYGFQGSMSKRGDCYDNAPIESFWGILKNELVH
                     HYNYQTREEAKADIIKYIELFYNHRRIQKGLGFKTPNQMAEDFYKLAA"
                     /name="IS3-like element ISAba22 family transposase"
     gene            complement(<6401..6505)
                     /locus_tag="SOI70_RS19050"
                     /old_locus_tag="SOI70_19075"
                     /pseudo=""
     CDS             complement(<6401..6505)
                     /locus_tag="SOI70_RS19050"
                     /old_locus_tag="SOI70_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436854.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family transcriptional regulator"
                     /name="CysB family transcriptional regulator"
     gene            complement(6573..7925)
                     /gene="gorA"
                     /locus_tag="SOI70_RS19055"
                     /old_locus_tag="SOI70_19080"
     CDS             complement(6573..7925)
                     /gene="gorA"
                     /locus_tag="SOI70_RS19055"
                     /old_locus_tag="SOI70_19080"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230226.1"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]; GO:0004362 - glutathione-disulfide
                     reductase (NADP) activity [Evidence IEA]; GO:0050661 - NADP
                     binding [Evidence IEA]"
                     /GO_process="GO:0045454 - cell redox homeostasis [Evidence
                     IEA]; GO:0006749 - glutathione metabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="WP_002122354.1"
                     /translation="MSKHYDYIAIGGGSGGIASVNRAAMYGKKCALIEMGDIGGTCVNV
                     GCVPKKVMWYSAQIADAIHKYGPDYGFNTELKSFNWKKLVENRQAYISRIHTSYDTGLA
                     KNNVDVIRGFAKFLDAQTIEVNGETYTADHILIATGTQAFKPEIEGAEYGIDSNGFFGL
                     EHLPKRTAIVGSGYIAVELAGVLNALGSNVQLFIRKHQPLRGLDHFLGKTLLENMRSEN
                     INVHTHSQINKVTKVQDNSLVLHLEDGTEHSVDCLIWAIGRKPATEAINLNKTQVQLNE
                     NRFIHVDAYQNTTQSGIYAVGDITGKVELTPVAVAAGRRLSERLFNNKPNEHLDYENIP
                     TVVFSHPPIGTVGLTEDQAIEKFGKDAIKIYSSSFTSMYTAITEHRQLTKMKLICVGTD
                     EKVVGIHGIGYGMDEILQGFAVALKMGATKKDFDNTLAIHPTSAEEFVTMR"
                     /name="gorA"
     gene            complement(8274..8444)
                     /locus_tag="SOI70_RS19060"
                     /old_locus_tag="SOI70_19085"
     CDS             complement(8274..8444)
                     /locus_tag="SOI70_RS19060"
                     /old_locus_tag="SOI70_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005804935.1"
                     /translation="MSNIIQLSKPCAFCDSRENVQLFAGLMLCGNCQENIRITNPGMFE
                     ANDQIEQKAQD"
                     /name="hypothetical protein"
     gene            complement(8441..8827)
                     /locus_tag="SOI70_RS19065"
                     /old_locus_tag="SOI70_19090"
     CDS             complement(8441..8827)
                     /locus_tag="SOI70_RS19065"
                     /old_locus_tag="SOI70_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032001883.1"
                     /translation="MNSTRTVAVRLDNQVAQEYERLAQLKGDKLGTYLRDLLTSNLQMA
                     SIERQANRIETLVNDFEKQINYSLDKFTEANYLNEKFFEDFGHIYMFQIGILMHLKVDR
                     GDIQGMQTKGNAYAQKNYFAGKKP"
                     /name="hypothetical protein"
     Typing          2..936
                     /name="rep_cluster_1233"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.466"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5203..6053
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.563"
                     /coverage="100.0"
                     /evalue="1.24e-164"
ORIGIN
        1 atgcctacca gtgaattaat agtaaaagat aatgctttaa ttaatgcgag ttataattta
       61 gataccgtag aacaacggtt aattctactg gctatcttac aagcaagaga aacaaaaaca
      121 gggattgatg caaacactag gctacggatc catgccagtg actatatgag cagatttaat
      181 gttaataagc atgccgccta caaagcccta aaaacagcag ttactaacct ttttggtaga
      241 cagtttagct atcaaacaat tgatgaaaaa acaggaaaat caaagaaagt aatttctcgt
      301 tgggttcaaa atatttctta tattgatgat gctgcaacct tagaggttac ttttacaatg
      361 gatgtagtac ccttaattac acgtttagaa aaacaattta cgagttatca gttaaaacaa
      421 gttacccaat taacaggaaa atatgccatt cgactctatg agttgcttat agcatggcgt
      481 gaagtcggta agactcctat atttgaagtt tctgactttc gttcgaagct tggcttaact
      541 tcagatgatt acccaagact agatacattt aaacgacgtg tacttgaatc agcagtcaaa
      601 cagataaatg aacatacaga tatcatcgtt aaagttgaac agcataaaga aggccgctct
      661 atttctgggt tctcttttag cttcaaacaa aaaagaaatc cgaacaaagt aatagaacgt
      721 aagaaagatc ccaatacatt agatattttt tccagaataa cagatgctca acgtcaccta
      781 tttgctaaca agctttcaga attacctgaa atggggaaat actctcaggg cactgaaagc
      841 tatcaacagt ttgcaataag aattgctgaa atgcttcaag atcctgctaa gtttcaagaa
      901 ttacatcctt atcttgaaaa agttggctat cgttaaacta ctgatgtaac gttacatgat
      961 taattattgt ctacaagcat acagattttt acttgggtta catgatgaaa gcactatctg
     1021 ttattgaatt atcacaactt tatgggatga atcgacaaag tatttataag cgtataaata
     1081 aaggagattt atcaaaaaat agtgatggga aaatagattt atctgaagcc atacgagtat
     1141 ttggcgaacc atctaataaa aataataatg taactacgtt acagtctact acggtacaaa
     1201 aagatacaga agtagacatg ctaaaacagc aagtagacat acttaaaaaa cagctagaac
     1261 ttgcacatga gcgagaagtt tttcaaagag aacaactaga agcaaaagat aatcaaatag
     1321 aagcaataca acgactgctg gaagccccaa aaaccaatat gactacgttt accgatccga
     1381 aacctagcca agatatagca acggatcctc ggtcagaacc tgagccgaaa catgacggat
     1441 tgactactcc agagcagagg gaaaataagc ggattccagt gccggagcaa gttgaacagg
     1501 aaccggagaa aaggggcttt tggagccgtt tttttagacc ttatgattaa ccacgattgt
     1561 ttaactaata tgaacaaagt cgtgaaacat tgcttaagta ataggcgtgg aacgtgaaaa
     1621 atcataaaaa aaacccaaac agtgatgttt gggctataaa ctttgaaata agtatttgaa
     1681 tttcaaaata aattataaca catttcgtat aaggtgtatt atgttaattt tagctttact
     1741 aaaactattt tttgagaact aggctgttat ccgacataga caccaacata ttctataaat
     1801 atctataaga ataagtaata gacagatatt tcttccaaac tcacaaatcc gtatctaatt
     1861 tatttcgata aaatcagctg gattaaagca tttaatgaat gttcataaat attttttttt
     1921 gaaagtcgat tcattaaaag agccccttta atggtcgaat ttaccataag agctaattcg
     1981 taattactta ctttagttat cattgaaata ttttcatcca aatcattttt aaaaatatgt
     2041 tcaataattc ttaactcact ttcaactaat tgaaagactg cgctctgtaa agtttcagag
     2101 aagctatcag tttctgtaag cattgaatga ataccgcaca tttctttttt ttcagcacat
     2161 tgtataaaaa aattacgata ttcaaacaac ttggcttcta tagaagcatg agaatttata
     2221 cgggtctcta gttctaaaag ctgattcaat ttaaaatgac aataggcaat tcccaaatcc
     2281 gtcttactag gaaaatgata atgaatactc gcctttttga tcccaatttc attagctaaa
     2341 tcagaatagc taaaagcatt aaaaccaact ttttgaatat atttgttcgc caattctaag
     2401 atttgattat acgttgaaat agtcattctc cttaattata ataatttatt atgataaata
     2461 agttttaaat caaattttaa caggactaaa ccagcaccaa ctaacagtac atctttaata
     2521 ataaaactat ctatatgacc taattgtgga ataatactaa gcgttgttat accagtcaag
     2581 ataactaaaa ggtcaccaat taagccaatt ttaggtttaa aaaaaccaat tattaacgca
     2641 ataaatgtaa tattttccac aacacctagg aaataacttg caccataatg accaaagagc
     2701 gtatatagaa tacttaacca agattttgat agtattggtt ctaaagcaac aacctcaaaa
     2761 tcgaaccatt tataggtacc aaataaaaca aaaatgaaga ttacacttaa ttttaatact
     2821 aaaatatctg cttgcttatt tatgaaatta cttaacaatt tacccatatt tattactcaa
     2881 catttttata atcctaatta gctaagccat tactattttt aaatttttta tctctattca
     2941 ttttgtagtt gaaatagaga tgtagataaa aataataata agaaaagtaa gatttagtgt
     3001 aggaactttt cacttagaat taccaattta tatgacttta gatgcaatct acctaccaaa
     3061 aggtagattg tcaagtctat aaatttaatt ttagacaatt tacaatattg acaatctacc
     3121 tattaatagg tagatttgat ttaaacaaaa cccagtttaa tcaaaattaa ggaataaata
     3181 atggaaacta aactcttctt acatgaccaa ttggtatcaa tgttattaat aacttcatta
     3241 tatagagact gtgttatata aaatttttat tttaaaatag tgggagattt ttgatatgtt
     3301 taactattta aaggaaaaaa actttattat tgctgaaaaa aacaaagggg aatttaattt
     3361 tatctcatta ttaagatggt ctcttgtaat tatatttata tggtttggag ggatgaaatt
     3421 tacacattat gaagcaattg gaatagctcc atttattgaa aatagcccaa ttgttagttg
     3481 gttgcataat ctatttggta ttcaaggagc tagctacttt ttgggttgca tggagttact
     3541 aactgcattt attttattac ttggtgcatt taagccaatt ttatctgcat taggtggttt
     3601 aatgtctgca ataacatttt taattactct aacatttatg cttacaacac caggtgtgat
     3661 agaaccgtct gctggaggtt ttcctgctct ttctggtgct ccagggcaat tcttattaaa
     3721 agatctagtt ttattagctg cttcattgtg cattttaaag gcatcaataa atcgaagaaa
     3781 tccttattaa ttttatgagt taataaaatt atattcaaaa tcaattaata atgaaaaggc
     3841 tattttatga cgtataagaa aacaattgag agtattgata aattaaatgt agatcaatat
     3901 cgtgtgacac aaaataatgg cacagaacgt ccatttgaag gtgaatatga taagcacttt
     3961 ttacctggta tttatgttga tattgtgtct ggtgagccat tattttcctc aacaaaaaaa
     4021 tataactcag gttgtggttg gcctgctttt tctaaaccta tagaaaatgt tactgagcat
     4081 gctgactttt cacatggaat gcgtcgtgtt gaggttcgct caaaacacgc aaatagccat
     4141 ctaggtcatg tctttacaga cggacctcaa tctgatggtg ggcttcgtta ttgtattaat
     4201 tctgcggctt tacgttttat tcctttgcta gaaatggaaa gacagggtta tgctgattat
     4261 ataaaatatg tggaggtaaa tacctaatgg aacgtgcaat attagctggt ggttgctttt
     4321 gggggctgca agatttgatt cgtaaattac caggtgttga acatacacgc gtaggttata
     4381 caagtggtga tattgccaat gcaacttacc gtaaccatgg tacacatgcc gaagcaattg
     4441 aaatcatatt tcattcaaat ataattagtt accgtaaatt acttgaattt tttttccaaa
     4501 ttcatgatcc aagtacgcta aaccgtcaag gtaatgatat tgggacaagt taccgctcgg
     4561 gaatttacta cactactgaa gaacaacatc aaattgcatt aaagaccatt caagatgtaa
     4621 attcttctgg gatttggcca ggtaaggtgc gaacagaagt aaaggcggca agtgattttt
     4681 gggaagctga gccagaacac caagattact tagagcgata ccccactgga tatacatgcc
     4741 acagagtccg tcctgattgg gttttgcctt aactatgatt ttttaatgca acagaacctt
     4801 ctagatgcac atccaagcct aatctgatgt gtgctgaaaa aacaggaggt tttaacttgg
     4861 taaactaaac acactcatca ggagtttacc atgagcaaga aacacaagac ttacaccaca
     4921 gaatttaaag ctgaagccat caaattaatt gaagccaatc aaggcaatgt ctcggaaaca
     4981 gctagacaac ttagcatttc aatgcaaact ctttcaaatt ggaataccaa agcaaaggct
     5041 ggaactttag caggtacaaa acagtattca cctgatctaa acgctctact cgaagaaaat
     5101 aaaaaactca aacaacagct caaaatagct gaaatggaac gtgaattttt aaaaaaggca
     5161 gcagcgtact ttgccaaaga aagtcagtaa ggtacgccta tatgaaacaa aaaagatatt
     5221 cttttccaat taccttaatg gctcgattac ttcatgtttc agtttcatgt ttttatgatt
     5281 ggctcaagaa aggcgtgagc aaaagaacga ttcaacgaaa tcaacagacg atattggtga
     5341 aaatagccca tgaggagaca aggcagagct atggttatat tcgattaacc aaatacttac
     5401 aagctcaggg tataaaaatg agtatgtacg ctgtacgtca gataaaagcg ctgaaccacc
     5461 tgtattgtaa gcgacacaag cgttttaaaa ggactacgaa tagtgaccat aatcgagcga
     5521 tctatgaaaa cctgctggag caacaattct caatgactag accaaatcaa gcatggtcaa
     5581 gtgatattac gtacatatgg actgttgaag gatggctgta tttagcagcg gtaaaagacc
     5641 tttacacgaa gcaagtggtt ggctatagct taaatgagcg catgacaaca cagcttgttt
     5701 gtaatgcgct aaatatggct attcacaatc aaaaaccaac caaagaactg attgtgcatt
     5761 cagacagagg aagtcaatat tgcagccatg aatatcgaaa tatacttgag caatatggtt
     5821 ttcaaggttc aatgagcaag cgcggagact gttacgataa tgcaccgatt gaaagctttt
     5881 ggggaatact gaaaaatgag ttagtgcatc attacaacta tcaaaccaga gaagaagcca
     5941 aagcagatat tataaaatac attgagttat tttataatca tcgaagaatt caaaagggtt
     6001 tgggttttaa gacaccaaat caaatggccg aagactttta taagttggct gcctagaatc
     6061 tcccaaggga aagtctcctg ataattcagc gtatatcatt atcaccaaaa aagttaatat
     6121 tttcattaag tagtagcata ttattaaaaa taatttgata aaaagtataa tagagaagtg
     6181 taaaattagc acttacatag ccactaattt atcaacattc gtcagatgac acataagttg
     6241 tcatttctga ttaaataata tcaatgagtt atagaattta gtgacaatca atgacattag
     6301 ttagtgacac ataagctgtc atttatttta ttttgttatt caatatctta aagtaaatat
     6361 ttcagaaaaa taaaaagata taataatgaa agaagacttt tttacttaaa ccagattgag
     6421 atgtttttag agcatgtgct gtttgtgata aattgagatt atgttgagtg agttctctaa
     6481 taattctaag ttgttgaaag ttcataattt ggatatagaa tataaaaata agagcaaata
     6541 tataaataaa tgctctttta gatgatcaat gattaacgca ttgtgacgaa ttcttcagca
     6601 gaagtcggat gaattgctaa agtgttatcg aagtcttttt tagttgctcc catttttaaa
     6661 gcaacagcaa agccttgcag aatttcatcc ataccgtatc caataccatg aattccgacc
     6721 actttctcat cagttcctac acaaattaat ttcatttttg tgagttgtcg atgttctgtt
     6781 atcgctgtat acatggatgt aaaggaagaa ctataaattt tgatggcatc tttaccaaat
     6841 ttttctatgg cttgatcttc ggttaatcca actgttccaa taggtgggtg actaaacacc
     6901 acagtcggaa tattttcata atctaaatgt tcatttggct tattgttaaa taaacgttca
     6961 gacagtcttc gacccgctgc cacagcaaca ggtgttaact cgactttacc tgtaatatca
     7021 cccactgcat aaatgccaga ctgtgttgta ttttgatatg catcaacatg aataaagcga
     7081 ttctcgttca attgaacttg agtcttatta agattgatcg cttcagttgc aggttttcta
     7141 ccaattgccc aaatcaagca atctacacta tgttctgtac catcttctag gtgtaataca
     7201 agtgagttat cttgaacttt agtcactttg ttaatctgac tatgagtatg cacgttaata
     7261 ttttcactgc gcatattctc taatagtgtt ttaccaagga aatgatctaa tccacgcaaa
     7321 ggctgatgtt tacgaataaa taattgtaca ttcgaaccca atgcattaag aacacctgcc
     7381 aattccacag caatatagcc agaaccaaca atagcagtcc gcttcggtaa gtgttctaat
     7441 ccgaagaaac cattggaatc aataccatat tcagcacctt caatttcagg tttgaatgct
     7501 tgagtccccg tagcaattaa aatatggtca gctgtgtacg tttcaccatt tacttcaatt
     7561 gtttgagcat ctaaaaattt tgcaaaacct cgaatgacat caacattatt tttagctagt
     7621 cctgtgtcat acgatgtatg tattcggctg atataggctt gtctgttttc aactaatttc
     7681 ttccagttaa aggatttcaa ttctgtatta aagccatagt caggaccata tttatgaatc
     7741 gcatcggcaa tttgagctga ataccacatc acttttttag gaacacatcc tacgttgaca
     7801 caggttccac caatatcacc catttctatc agtgcacatt tcttaccata catggcagca
     7861 cgattgactg atgcaatacc gccactgcct ccgccaatcg caatataatc atagtgttta
     7921 gacataaatg aaattcctgt tttttagatt taaaaagtta aagacttaag cattcatttg
     7981 aatgacaaat ctaaacttaa catctcctgc tcttaaacgt tgtaaagcat cattaacacc
     8041 tactaacggt acagtttcta tttcaggacg aatatttgct aatagactaa aatcgagcgt
     8101 tttttctgaa tcgtgaggtg ttcctgttaa agagccttga attttactta tacagtaata
     8161 acaaatagac ttgcgaatac taagttatat agaacctgct agcaaattag gggtggttat
     8221 aaagcgaact tatagaaaac atttaacata aaatctctta tacgcagccc aggttaatcc
     8281 tgggcttttt gttcaatctg atcattggcc tcaaacatgc caggattagt aatccggata
     8341 ttttcctgac aattcccaca aagcatcagc cctgcaaaaa gctgtacgtt ttcccttgaa
     8401 tcacagaaag cacacggttt agaaagttga ataatattgc tcacggcttt ttccccgcaa
     8461 aatagttctt ttgggcatat gcatttcctt ttgtttgcat tccttggatg tctcctcgat
     8521 cgacttttag atgcatcagt atcccaattt gaaacatata tatatggcca aagtcttcaa
     8581 agaatttttc atttaaataa ttggcctcag taaacttatc gagggagtaa ttaatttgct
     8641 tctcaaaatc attcaccaat gtttcaatcc gattggcctg ccgttcaata ctggccatct
     8701 gtaaattact ggtcagaaga tcccgcagat aagtccctaa tttatcaccc ttcaactgag
     8761 ccaaacgctc atattcttgt gcgacctgat tgtctaatct gacggcaacg gttcgagtac
     8821 tgttcatgaa aaactcctac aatttgtttt caatctaagc acctaatttt taaaaagtaa
     8881 ctatcaaata taagtaaaca ctgtgttaag ttttaatgat tacaggttaa atatctaata
     8941 taaaaagaga aattcatata aaagcgtaaa caaaggtgat tacaagctat cctattgttt
     9001 tgtaagtaaa tattgcactt tatttgcaac aaaacgtgtt tacagataaa cctatgaaaa
     9061 ataataaaaa tcatgctaaa agcgtaaacg ctttccagat agatgaaaaa tcgctgtatc
     9121 ccttatagga taaggctttg gagcctttat ctttagatgc gtggtgtgtg aaccaaaaat
     9181 tgccggtatg taccggctcc caatccattt aattgactta actgacagta aagatagtgc
     9241 ttgaccaaaa gagatcctgg ctattgtgtg tctaagggaa aaaatctata agcggaaact
     9301 ctagcctaca gatcaaaaat aaaacccaag ttgaaacgct ctcacatgcg atttcagcca
     9361 catttttaca taagacatag aatcgactat tgaaatgagt aatcaggtca aaaaacgcaa
     9421 atgagcgcca aatagagact atttttattc taatcgggat gaatagagcg agtgtctacg
     9481 agcgaagcac caaaattcgc acctcgactc tctgaaaaac agctttttag gctgtgaagc
     9541 ctaaataagc gtaaatcaca tgcagaaaat tgggcctaga tcgagcgtag cgagtaaaaa
     9601 agctttatga gcgaagcgaa ttccgagttg cttttgcttt ttctaaaagt cacagctgga
     9661 agagctaaaa aattgcccca ggaatcgagc gaaagcgaga ttcaatagag tttgagcgta
     9721 gcgaaaacca agggcaattt ttccctccta aggcttttaa ttattttaaa gtttttaaat
     9781 gcttttagat ctgctgtaag tctttattta tagtatatac aagggatata ccacaccaaa
     9841 aagtcaccta taccacacca aaaagtcacc tataccacac caaaaagtca cctataccac
     9901 accaaaaagt cacctattta caccattgac taataatggt gtaataacac cacgtataat
     9961 aaaggtgtag caa
//
