LOCUS       NZ_CP139260            10476 bp    DNA     circular CON 06-DEC-2023
DEFINITION  Acinetobacter pittii strain HUM3 plasmid pApiHUM3b, complete
            sequence.
ACCESSION   NZ_CP139260
VERSION     NZ_CP139260.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38315710
            Assembly: GCF_034066905.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10476)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S., Guerrero,G., Garza-Gonzalez,E.,
            Lozano-Zarain,P., Volkow,P., Morfin-Otero,Md.R. and Cevallos,M.A.
  TITLE     Acinetobacter pittii: intercontinental dissemination, pathogenetic
            potential, and antibiotic resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10476)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S. and Miguel,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2023) Programa de Genomica Evolutiva, Centro de
            Ciencias Genomicas, Av. Universidad s/n, Col Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: HUM3_genome
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 993x
            Sequencing Technology :: DNBseq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/06/2023 00:00:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,813
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,632
            CDSs (with protein)               :: 3,632
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 35 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 17 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139260.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10476
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="HUM3"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pApiHUM3b"
                     /geo_loc_name="Mexico: NuevoLeon, Monterey"
                     /lat_lon="25.6888 N 100.3502 W"
                     /collection_date="2021-06-17"
                     /collected_by="Hospital Universitario Dr. Jose Eleuterio
                     Gonzalez"
     gene            join(10403..10476,1..448)
                     /locus_tag="SOI70_RS18940"
                     /old_locus_tag="SOI70_18965"
     CDS             join(10403..10476,1..448)
                     /locus_tag="SOI70_RS18940"
                     /old_locus_tag="SOI70_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999915.1"
                     /translation="MNDSDDRNHKVILEYLQKVLERERDRFYKLDDKVSKLFTTVSTVI
                     TGFLIIVNILLDHNKISIFCSSEITILSLLSLTTFCFLCMSWNFLFKVFNLKKTAHLQH
                     QGFWKYYKNREEIDFYERIIKDHETVIDTYIESNDQKVLLLKKSLSNIYLSAIMFVILV
                     IIYLIFKYLK"
                     /name="hypothetical protein"
     gene            445..591
                     /locus_tag="SOI70_RS18945"
                     /old_locus_tag="SOI70_18970"
     CDS             445..591
                     /locus_tag="SOI70_RS18945"
                     /old_locus_tag="SOI70_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000192351.1"
                     /translation="MTQKNSNTSTNPANQQPHKDIKSEVPPPPVRYGLDEKIKAEVPKS
                     NKK"
                     /name="hypothetical protein"
     gene            complement(639..929)
                     /locus_tag="SOI70_RS18950"
                     /old_locus_tag="SOI70_18975"
     CDS             complement(639..929)
                     /locus_tag="SOI70_RS18950"
                     /old_locus_tag="SOI70_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_000176412.1"
                     /translation="MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTV
                     PDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDNNELILARIGSHSELFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(913..1188)
                     /locus_tag="SOI70_RS18955"
                     /old_locus_tag="SOI70_18980"
     CDS             complement(913..1188)
                     /locus_tag="SOI70_RS18955"
                     /old_locus_tag="SOI70_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140357.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_005140357.1"
                     /translation="MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQV
                     VNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQELFTDLEDDTDD"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(1193..1348)
                     /locus_tag="SOI70_RS18960"
                     /old_locus_tag="SOI70_18985"
     CDS             complement(1193..1348)
                     /locus_tag="SOI70_RS18960"
                     /old_locus_tag="SOI70_18985"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_158000192.1"
                     /translation="MCYNGFSQINELTTELTTELTTELTTELTTELTTELTTELTTELT
                     TELTTG"
                     /name="hypothetical protein"
     gene            complement(1436..1675)
                     /locus_tag="SOI70_RS18965"
                     /old_locus_tag="SOI70_18990"
     CDS             complement(1436..1675)
                     /locus_tag="SOI70_RS18965"
                     /old_locus_tag="SOI70_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008486742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017482479.1"
                     /translation="MSEQQIYERIRLAMNEAPRNRQTAELHLQMIKYADDLKNITSKEF
                     CEGVGLPLSYGTEFSKMRNITERLKAAGLKVNMI"
                     /name="hypothetical protein"
     gene            complement(1901..2218)
                     /locus_tag="SOI70_RS18970"
                     /old_locus_tag="SOI70_18995"
     CDS             complement(1901..2218)
                     /locus_tag="SOI70_RS18970"
                     /old_locus_tag="SOI70_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065053.1"
                     /translation="MLGENHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            complement(2567..3097)
                     /locus_tag="SOI70_RS18975"
                     /old_locus_tag="SOI70_19000"
     CDS             complement(2567..3097)
                     /locus_tag="SOI70_RS18975"
                     /old_locus_tag="SOI70_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_017482483.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSQNKIDLAEAIRV
                     FGEPVKKQDVKEPVKIDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EGPKPNMTTFTDQELVQPSIAKPDPEPDTAEAKHDGLTTPERRENKWIQVPEHIEPEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(3176..4105)
                     /gene="repM"
                     /locus_tag="SOI70_RS18980"
                     /old_locus_tag="SOI70_19005"
     CDS             complement(3176..4105)
                     /gene="repM"
                     /locus_tag="SOI70_RS18980"
                     /old_locus_tag="SOI70_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005065049.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGFAPA"
                     /name="repM"
     gene            complement(4805..5518)
                     /locus_tag="SOI70_RS18985"
                     /old_locus_tag="SOI70_19010"
     CDS             complement(4805..5518)
                     /locus_tag="SOI70_RS18985"
                     /old_locus_tag="SOI70_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006581718.1"
                     /translation="MAGRKTFALTQQEIIDLDKHIRALPFKRRMQDAFLAMISERESMK
                     QLNEEDLKLIQRCRYERNTYNKRIVQLQLIQKTEPQKLKKLELNILELSKKTDIDAYFA
                     MHDALNEVLKIKRNQTSEKNITAKVERVLNPEKYNQAPLSKSLRKQQDHIKYFIGSLYL
                     NLLEQSGLERSNSNQDLDRMLDLFKQAQSGAEKNNAFSLDQNRKTDILSAKSLYEMEKL
                     IKQAKEDPRNPFNKA"
                     /name="hypothetical protein"
     gene            5974..6858
                     /locus_tag="SOI70_RS18990"
                     /old_locus_tag="SOI70_19015"
     CDS             5974..6858
                     /locus_tag="SOI70_RS18990"
                     /old_locus_tag="SOI70_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_017482471.1"
                     /translation="MFYLNLKHNIKKEQSSLNAFHYLTRTAHFENQKDNEKLEFIRYGN
                     MPKWAENKPKLFWKSADQFEISRGRTSSTLTIALPKELIREQRAELVQKLIDQFAGQYQ
                     FPYTAVIHNHPSEITGEDQPHLHLMYSERTISDDIERPPEQFFKQYRPKNPTQGGAQKL
                     TADALGFGRNQVKVFREKTQELMNLFLEKYAPTKKVMVYGVEIEVKNQVSCLSNDDFNQ
                     KFGTKLQDVPQIPRWKLYSADPIIQLDVEAQKNTIKKIRNQNNAELYGKEYDLEISRRH
                     VLTQKKDPGFSLD"
                     /name="MobA/MobL family protein"
     gene            7175..8257
                     /locus_tag="SOI70_RS18995"
                     /old_locus_tag="SOI70_19020"
     CDS             7175..8257
                     /locus_tag="SOI70_RS18995"
                     /old_locus_tag="SOI70_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482472.1"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_017482472.1"
                     /translation="MNSSNSHFNEVDPTLINETIPLFEKVPFNPIYPSKFKFIDLFAGI
                     GGFRLAFQNLGGRCVFSSEIDLAAQKTYTANFGDTPHGDITLESTKDAIPDNFDILCGG
                     FPCQAFSIAGKRSGFEDTRGTLFFDVADIIKRKQPKAFFLENVKGLVSHDKGKTLKTIL
                     QVLREDLGYYVPEPKIINAKDFGVPQNRERIFIVGFHKNTGVQDFEYPTPTGKKVCFAD
                     VKEENVVPTKYYLSNQYLQTLERHKAHHESKGNGFGYAIIPDNSIANAIVVGGMGRERN
                     LVIDHRITDYTPTTRIKGEVNRQGIRKMTPREWARLQGFPDNFKIIVSDAAAYKQFGNS
                     VAVPAIQATANLILEKLKMK"
                     /name="DNA cytosine methyltransferase"
     gene            8257..9348
                     /locus_tag="SOI70_RS19000"
                     /old_locus_tag="SOI70_19025"
     CDS             8257..9348
                     /locus_tag="SOI70_RS19000"
                     /old_locus_tag="SOI70_19025"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpaII family restriction endonuclease"
                     /protein_id="WP_017482473.1"
                     /translation="MITANKGEWSEVYALFKLLSEGKLYAGDKDLNKIPNLIYPIISIL
                     RQESQDLLTYSPEPNHGVVHIQNGTSTFTISQSDFKCITEFLLTEIKKKQPTASFSIPE
                     VETFISQYNSKKIKAKSSAKSDIRIIIYDQKIGTTPELGFSIKSKLGKASTLLNASQAT
                     NFVYKVKNLTLSPQEIDDFNQLEFTVPIIQGRIHHLESLGGSIEFSHITNDVFNNNLIL
                     LDSLLPNIISEALYKYYTSPITSVKEIFEQVASANPMHYSLQHQHPFYEYKVKKLLCEI
                     AIGMMPSTVWTGNNIDATGGYLVIKEDGDVICYHLYHRHEFEEYLYNNTRFEAASKSRH
                     NYGNLFIENGELYMTLNLQIRFK"
                     /name="HpaII family restriction endonuclease"
     gene            9400..9867
                     /locus_tag="SOI70_RS19005"
                     /old_locus_tag="SOI70_19030"
     CDS             9400..9867
                     /locus_tag="SOI70_RS19005"
                     /old_locus_tag="SOI70_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004727917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="WP_017399702.1"
                     /translation="MIEKGAIISDCNKYRYSLWRIWDKEKPIFTFVGLNPSTADHEQDD
                     RTIRRCINFAKSWGGGGIYMANLFAYRATDPQEMMSQEDPIGPDNDAHLKQLAAESHKL
                     IACWGNDGVFKARSTFVKQLLTDKLYYLKLNKSGEPGHPLYIHSSTQIQPF"
                     /name="DUF1643 domain-containing protein"
     Typing          complement(3190..4105)
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.884"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5975..6855
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.98"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgattttat aaattagatg acaaagtatc taagttattt acgacagtat ctactgtgat
       61 tacaggattt ctaattatag ttaatatcct tttagatcat aataaaatta gtattttttg
      121 ttcttccgag attactattc taagtttatt gtcacttaca acattctgct tcttgtgcat
      181 gtcttggaac ttccttttta aagtttttaa tttaaaaaaa acagctcacc ttcaacatca
      241 agggttttgg aaatattata aaaatagaga agaaatagat ttttatgaaa gaatcattaa
      301 ggatcatgaa actgttatag atacatacat agaatctaat gaccaaaaag ttttactttt
      361 aaagaagtct ttaagcaaca tctatttatc agcaataatg tttgttatat tagttattat
      421 ctatttgatt tttaagtatc taaaatgacc caaaaaaatt ccaatacaag taccaaccca
      481 gctaatcaac aacctcacaa agatatcaaa tcagaagttc cacctcctcc tgttagatat
      541 ggtttagatg aaaaaattaa agctgaagtt ccaaaaagta ataaaaaata atctctaatt
      601 attgacaata aaaagcccga atatatcggg ctttttactt atccaaaaag ttcagaatga
      661 gatccaatcc gagcaagtat tagctcatta ttatcttcat ccacatccca aattaataat
      721 aaatcaggta aaatatggca ttccatatgg ggtttccact caccaatcaa aggatgaggt
      781 aaatactcat ctggaacagt ccctgttttt accaaatgct catcaataag atccttcaaa
      841 attaagagtc ctttcttaag ctttgggttt gagcttaccc gtttaaaatc ttttttaaat
      901 tgtttcttgt acctaatcgt cagtgtcatc ttctaaatcc gtaaacaatt cctgagcaga
      961 actaaatttt ttaggttctg caacagagtt catagcctca attgttgtac ggttaggggt
     1021 tcttaactct aaaggaaatc cacctcgatt tactacttgc gttaataata agcgaatagc
     1081 atctgtagta tttaaaccca cttcttttag aattcgctcc gcttgaacct ttaattcatt
     1141 tggaacccta gcacgtacat actcatcttt ttttgccaaa gtattcatat gtttatccag
     1201 tagtcagttc agtagtcagt tcagtagtca gttcagtagt cagttcagta gtcagttcag
     1261 tagtcagttc agtagtcagt tcagtagtca gttcagtagt cagttcagta gtcagttcat
     1321 tgatttggct aaagccattg tagcacacat gtgctacaca acaactaact taacataaaa
     1381 tctcttatgc gaaatttaaa agggcttagt catgactaag cccttttatt agcttttaaa
     1441 tcatatttac cttaaggcct gctgctttta agcgttcagt aatgtttctc attttactaa
     1501 attcagtacc gtaactaaga ggtagtccga caccttcaca gaactcttta gatgttatat
     1561 tttttaaatc atctgcatat ttaatcattt gcaaatgtag ctctgccgtt tgtctgtttc
     1621 taggagcttc attcatagcc aatcgaatac gctcatatat ctgttgctca ctcatatcta
     1681 atttatccaa ttaatcaaat ttatataaaa aatataattt atataaattt gattgtcaat
     1741 tttttgttca atcttattgg cgaaagatga caaatatgat taaaaaaaag ccgacatgtg
     1801 tctaagtcgg cattttggtg ttttttaggg tggcgaaaaa tttttaacta tatgaaaaac
     1861 aacaatttta taaaaacaag ttcgtataac gcccattatg ttaaatggaa tgtttatgag
     1921 aaattaatag tctatttttg tttgagattg aagtatattt aaaaaagtta agttgttttt
     1981 ttaaatgcat aacttcatct tttagttttt tgatttgttg ctctctttcg tcctgtatag
     2041 ggtaagtaag ttcaccaaat tttctaacta actgactgta ttcaatcaat ttattgtagt
     2101 caagttcgaa atctccgctt tttatatatt taagtatagt cgagcgaggt tttttatacg
     2161 ctatagcagc atcactgatc gaaaattttt ttaaaaaatg atgattttca cctagcatac
     2221 tatttcactt aaggcattat taatttcgta tatatatgtt aataaaatta acacatacaa
     2281 gatataaatc tttaatcatt attggttaat aatcgaacgt ttactttaca catgccctac
     2341 tctgataatt tttctattat taaatcaata tgcttttaaa aatttatatt attaacataa
     2401 tacaccttat acgaaatgcg ttataattct cttgaaaatt caacgctgta gtttcttttt
     2461 tgcccaacct ccccaaggtg ggcttttttt tatgattttt cacgttccac acttattatt
     2521 taatcaaagt ttcacgactt tattcatatt aattaaacaa tcgtggttaa tcatagggtc
     2581 taaaaaaacg gctccaaaag cctcttttct caggctctgg ttcaatgtgc tctggcactt
     2641 gaatccactt attttccctc cgctctggag tagtcaatcc gtcatgttta gcttctgctg
     2701 tgtcaggttc gggatctggt ttggctatgc tgggttgcac taattcttga tcggtaaacg
     2761 tagtcatatt aggttttggg ccttctaata ggcgttgcat cgtttcaatt tggttctgat
     2821 agaaagattc ccgatctttg gcatcatcaa gctggttttt cagcatgtct atctgctgtc
     2881 taagtagtaa aacttctgcc gaatttggac tgtctatttt tacaggttct tttacatcct
     2941 gttttttgac aggttccccg aaaacacgga tagcctcggc tagatcgatc ttattctggg
     3001 aatttttact taaaattcct ttctttatgt tgttgtaaat cgtttgtcta ttgatattgt
     3061 aaagcttaga aagctcgaga actgtaaggc ttttcatgtt gtaaatctat gcctaaacct
     3121 gtcaaaagtt gttgtctatt agactgtcta atagacaaat gatcaagttt aaattttagg
     3181 caggtgcaaa gccaactttt tgaagataag ggaataattc ttggaatttt tgataatctt
     3241 ggagcatttc tgcaatgcgt atagcaaatt gttggaagct ttcggtgcct tctgaatatt
     3301 gccccatctc aggtaattca gaaagtttgt tggcaaagag atgacgttgt ttatcactca
     3361 ttttggtgaa aagggctaat gtatccgttc ccttaattac tttgtcagag ttggtttttt
     3421 tctgcttaaa actgaaagaa aaaccggaaa ttgaacgtcc ttttttatgt tgttcatatt
     3481 tagccgttat atcggttaac tcatttactt gcttgagggc aaaatctaaa acatacgttt
     3541 taaaattact cattgttttg tattgatgag cttcgacacc aagttgttgt ctaaagatcg
     3601 atagatcaaa aattggcgtt ttaccagcag atctccattg aattaataac tcgtataacc
     3661 gaatagcata agagctagtt aatctcgaaa cctgctccaa ttcatattta gtaaagtttt
     3721 cttccagtcg agttatgagt ggaacaatgt cttgcgtgaa acgtaggaaa acgatcccgg
     3781 attgaggttc atatccaatt ttatcgaccc aacgacagtg aaagctacga tccttaccag
     3841 tcttaggatt aatgtcatga tacgtgacat agcgatcaaa caagctttta gatgcatccc
     3901 gaagcacggt ataagcagtg tgtttctcga cattaaaggt attgatataa ctacttgcat
     3961 gtacttctaa aaggctattt tcagttatcc cgtgccctgt ttcccgagct tcagcaatgg
     4021 ctaataggat cagcctttgt tcaactgtat ctagggtata gctagcttga attaaagcat
     4081 tatcctttac gattaattca ctcatttcta ataataaatt tataattaaa ctaggttgta
     4141 caatagacta ttttaattga attgtaaatc gacttaattt ggggtcgata aacctagttt
     4201 taaggtcgat aaacctagtt ttatgggtcg ataaacctag ttttatgggt cgataaacct
     4261 agttttatgg gtcgataaac ctagttttat taactctaaa actattatga ataaagggtt
     4321 acagcttact taaaagcatt taaaagatta aaaataatta aaagcctagc aaaagaaaaa
     4381 ttgcccttgg ttttcgcttc gctcaaactc tattgaactt cgctttcgct cagttcgtcg
     4441 gggcaatttt ttggtgagca cttacgtgac tttaaaaaaa gcaaaagcaa ctcggaattc
     4501 gctacgctca taaaactttt ttactcgcta cgctcgatct agactcaaat ttctacaact
     4561 tttccatgct tatttatgtt tcacagccta aaaagcggtt tttcagggag tctaggcaaa
     4621 attttccaag gatcgctcgt agacactcgc tttgttctta tcggtaaaat caaatgatct
     4681 ctaaaatact ctaatttgcg tttagagcac tctaattttt ttattgatgg ggaatatagg
     4741 tttaggctag aaaaaggcat atacggcttc aacagtgtat ttaaacccta ttatttgagt
     4801 caatctaagc tttattgaaa ggattccttg gatcttcttt ggcttgctta atcaatttct
     4861 ccatttcata tagcgattta gccgataaaa tatctgtttt tctattttga tccaggctaa
     4921 atgcattgtt cttttctgct cctgattgtg cttgcttaaa taaatcgagc attcgatcta
     4981 aatcttggtt tgaatttgat cgctctaaac cgctttgttc caataagttt aaatatagtg
     5041 atccgataaa atatttaatg tggtcttgct gttttcttaa agatttacta aggggggcct
     5101 ggttatattt ctcagggttt aaaactcgtt cgaccttcgc tgtaatattc ttttcagaag
     5161 tctgatttct tttaattttt aaaacttcat ttaatgcatc atgcattgca aagtaagcat
     5221 caatatctgt ttttttactt aattctagaa tattcaattc taattttttt aatttctgtg
     5281 gctcggtttt ttggatcagt tgtaattgaa ctatacgttt attataagta ttgcgctcat
     5341 aacgacatcg ttgaataagt tttaaatctt cttcattcag ttgtttcatt gattctcgtt
     5401 cagaaatcat tgctaaaaaa gcatcttgca ttcgtctttt aaacggcagc gctcgaatat
     5461 gtttatccaa atcgatgatt tcttgttgtg ttaaggcaaa tgtttttcta ccggccaaag
     5521 ctgatttcct gattttgggt atctaaattg atgttagcgg aattctgatg gtgttgaaac
     5581 aatattttat gttatacata aaatattgtt tatctcgttt ccgtgtcttt ttttaacaat
     5641 gattgcttta gcaatcattg ttaaaaaaag acacttctac tcgctcctgc ggaggctaca
     5701 taacccactg atttaattat taaattatga gtttttcttc gggttttaac tgtatttttt
     5761 ttaatggtga aaaagtaagc aatgcctgcc gttacaagaa atgggaaagg ggtgcaacaa
     5821 gaaaaacagc gaattttaga cgagtgcaac aaactttttt gatgaaatat cgatgtttgc
     5881 aacaagaaaa gactgctctc tgcaacaaga attagcccct tgttacaaga aaatttgctg
     5941 tttcgttaca cgtaatttgt ttgaggtgaa ctgatgtttt atttaaatct aaagcataat
     6001 attaaaaaag agcagagttc tctgaatgca ttccattatt taactcggac ggcacatttt
     6061 gaaaatcaaa aagacaatga aaaattagag tttatacggt atggaaatat gcccaaatgg
     6121 gcagaaaata aaccaaaact tttttggaaa agtgctgatc aatttgaaat ttcccgggga
     6181 agaacaagct ctactttaac cattgcttta ccaaaagagt taatacgaga acaacgagca
     6241 gaattggttc agaagttaat agatcagttt gccggacaat atcaatttcc ttataccgcg
     6301 gtcattcata atcatcccag tgaaattact ggagaggatc aaccacattt acatttgatg
     6361 tattcagaaa gaacaatttc agatgatatt gaacgaccac cagagcagtt ttttaaacag
     6421 taccggccaa aaaatccaac tcaaggtggt gcgcaaaagt taacagctga tgcattaggt
     6481 ttcgggcgaa atcaagtcaa agtattccgt gaaaaaactc aagaattaat gaatttgttt
     6541 ttagaaaaat atgcaccaac taaaaaagta atggtttatg gtgtagaaat tgaagtgaaa
     6601 aatcaggtca gttgcctatc taatgacgat ttcaatcaaa aattcgggac caaattacag
     6661 gatgtaccac aaatccctcg atggaaactt tatagcgctg atccaattat tcagcttgat
     6721 gttgaagctc aaaaaaacac aattaaaaaa attagaaatc agaacaatgc tgaactttat
     6781 ggcaaggaat atgatttaga aatatctaga cgccatgtgc ttactcagaa aaaagatcct
     6841 ggctttagct tagattgatt tttataagtg gaaaatttgt gttttttgaa catgaaatct
     6901 tgaaaaaact tatccatatt gtgagtgatt tttagccaca acatggaaaa tttgtgtttt
     6961 tgattcagat ccataaggaa aacctagatt tttgcccata aatgtaaaat ctaggttttt
     7021 cgcataaggc gtattatgtt aattttagaa aaattaaatt attcgcttta tgatccatat
     7081 atgaacaaca cgacaatata tgtcctatga ccaaagaaat tattgcctaa ctttgctatt
     7141 gttcttcttt tgatatttgt acttaaggcc tgtaatgaat tcgagtaata gtcactttaa
     7201 tgaagttgat ccgactctaa ttaatgagac aattcctctt tttgagaaag tgccatttaa
     7261 tcctatttat ccgagcaaat ttaagtttat tgatctcttt gcaggaattg gtggattcag
     7321 attagctttc caaaatcttg gtggccgatg tgtattttca tctgaaattg atttagcagc
     7381 gcagaaaact tatacggcaa attttggtga tactcctcat ggagacataa cattagaatc
     7441 aaccaaagat gctatcccag ataattttga catcttatgt ggtggatttc cctgccaagc
     7501 tttctctata gctggtaaaa gatctggttt tgaagatact cgaggaactc tattctttga
     7561 tgtagcagat attataaaaa gaaaacagcc taaagccttt ttcctagaaa atgtaaaagg
     7621 tttagttagc cacgataaag gaaaaacttt aaaaactatc ttacaagtac tgagagaaga
     7681 tcttggctac tatgttcctg aacctaaaat tattaatgct aaagatttcg gtgttcctca
     7741 aaaccgtgaa cgtattttca ttgtgggatt tcataaaaat actggtgttc aagattttga
     7801 atacccaaca ccaacaggta agaaagtttg ctttgctgat gtgaaagagg aaaacgttgt
     7861 tcctacaaaa tattatcttt ctaatcaata tttacagact ttagaacgac ataaagccca
     7921 tcatgaaagc aaaggaaatg gctttggtta tgcaatcatc ccagataact ctatagctaa
     7981 tgctattgta gtcggaggta tggggcgtga acgtaatctt gttatagatc atcgaattac
     8041 ggactatacg cctacaacga gaattaaagg tgaagtaaat agacaaggta tccggaaaat
     8101 gactccccga gaatgggcac gcttacaagg attccctgat aattttaaaa ttattgtgtc
     8161 tgatgccgct gcatataagc agtttggtaa ttctgttgct gttccagcga tacaagctac
     8221 agctaatttg attctagaaa aactgaagat gaaataatga ttacagcaaa taaaggtgaa
     8281 tggagcgaag tctacgctct attcaaactg cttagtgagg gaaaactata tgcaggggat
     8341 aaagacttaa ataaaattcc taacttaatt tatccaatta ttagtatttt acggcaggaa
     8401 tcgcaggatc ttcttacata ttccccagag cctaatcacg gggtagtaca tattcaaaat
     8461 ggtacttcta ccttcacaat tagccaatct gattttaaat gcatcacaga atttctttta
     8521 actgaaataa agaaaaaaca accaacagca tccttttcta ttcctgaagt tgaaaccttt
     8581 atatctcaat ataattcaaa gaaaatcaaa gctaaatcca gcgcaaaaag tgatattaga
     8641 attatcattt atgatcaaaa gatcggaaca actccagagc taggttttag tattaaatcc
     8701 aaattaggca aggcttcaac gctacttaat gctagtcaag ccaccaattt cgtttataaa
     8761 gttaagaatc ttactttgtc accacaagaa attgatgact tcaatcaatt agaatttact
     8821 gttcccatca ttcaaggtcg tatacaccat ttagaatcgt tggggggaag tattgaattt
     8881 tcccatataa caaatgatgt ttttaataat aatttaattc ttttagatag cctacttccg
     8941 aacataattt ctgaagcact ttacaagtac tacacctctc caattacatc cgtaaaagaa
     9001 atatttgaac aagttgcatc tgcaaatcca atgcattatt cactacaaca tcaacaccct
     9061 ttttatgaat acaaagttaa aaaactttta tgtgaaatag ctataggaat gatgcctagc
     9121 acagtttgga ctggcaataa tattgatgca actggtggtt atttagtgat taaagaagat
     9181 ggcgatgtta tttgttatca cctatatcat agacatgaat tcgaagaata tttatataac
     9241 aacacaagat ttgaagcggc tagcaaatcg agacacaact atggaaatct attcattgaa
     9301 aatggagagt tatatatgac cttaaattta cagattaggt ttaagtaaga tatttaacat
     9361 aaaaactctt attcgaaata gatcaagaag gacaatgtag tgatagaaaa aggggcaatc
     9421 atttccgact gtaacaaata tcgttattca ctttggcgta tttgggataa agaaaaacca
     9481 atatttacct ttgtcggctt aaatccatca actgcagacc atgagcaaga tgaccgaacc
     9541 ataagacgat gcattaattt tgctaaaagt tggggaggtg gtggcattta tatggctaat
     9601 ctatttgcat atagagctac tgatccacaa gaaatgatgt cccaagaaga ccctattggt
     9661 cctgataacg atgcccactt aaagcaatta gcagcagaat cccacaaact tattgcttgt
     9721 tggggaaatg atggtgtttt taaagcgaga tcaacttttg taaaacaatt gcttacggac
     9781 aaactctatt atcttaaatt aaataaaagt ggagaacctg gacatccgtt atatatacat
     9841 tcttccactc agatacagcc tttttagatc ccatttaaca taatgggcgt tatccgaaat
     9901 cgtcttttat aagttaaata aaatcatgta cttagctatt ttacataacc cgaaactacc
     9961 gcaaaaatcc gctttgtttc caaagcggat tttttttgag caaggttgat acttcttgcc
    10021 aataaaattg gttaaataat gatcaaaaat agcaattcca tagagttttt aagccttttt
    10081 tacccaaaag aatccacaat ttatgcggat aaccattggg ataaattttg attatttttt
    10141 gtacgttttg gcttacagcg ttttacgaaa ttgtctgtat ctatcccccg atgaagtcct
    10201 tatgatcaat ctatatgaac aagatgttca atgacttaaa acaacaataa taacaataat
    10261 aatcagtcag gcaccatgac catcaggtag tcagccctac ctaggcgctc cacaccgaaa
    10321 aatcccgaca tgatgtcggg attttttatt gttaaaattt aaattaaata aattttgtta
    10381 tttgaaataa aggggattga gtatgaatga ctctgatgat cgtaatcata aggtaattct
    10441 tgagtatcta cagaaagtac tagaaagaga gcgaga
//
