LOCUS       NZ_CP139256            11158 bp    DNA     circular CON 06-DEC-2023
DEFINITION  Acinetobacter pittii strain MCR16048 plasmid pApiMCR16048b, complete
            sequence.
ACCESSION   NZ_CP139256
VERSION     NZ_CP139256.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38315711
            Assembly: GCF_034066105.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S., Guerrero,G., Garza-Gonzalez,E.,
            Lozano-Zarain,P., Volkow,P., Morfin-Otero,Md.R. and Cevallos,M.A.
  TITLE     Acinetobacter pittii: intercontinental dissemination, pathogenetic
            potential, and antibiotic resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Bello-Lopez,E., Escobedo-Munoz,A.S. and Miguel,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2023) Programa de Genomica Evolutiva, Centro de
            Ciencias Genomicas, Av. Universidad s/n, Col Chamilpa, Cuernavaca,
            Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: MCR16048_genome
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 423x
            Sequencing Technology :: DNBseq; Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/05/2023 23:39:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,764
            CDSs (total)                      :: 3,672
            Genes (coding)                    :: 3,611
            CDSs (with protein)               :: 3,611
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 29 of 61
            Pseudo Genes (incomplete)         :: 28 of 61
            Pseudo Genes (internal stop)      :: 8 of 61
            Pseudo Genes (multiple problems)  :: 4 of 61
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139256.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="MCR16048"
                     /isolation_source="Endotracheal tube discharge"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pApiMCR16048b"
                     /geo_loc_name="Honduras: Cortes, San Pedro Sula"
                     /lat_lon="15.5241 N 88.0411 W"
                     /collection_date="2015-08-25"
                     /collected_by="Hospital Catarino Rivas"
     gene            join(10345..11158,1..116)
                     /gene="repM"
                     /locus_tag="SOI72_RS18720"
                     /old_locus_tag="SOI72_18720"
     CDS             join(10345..11158,1..116)
                     /gene="repM"
                     /locus_tag="SOI72_RS18720"
                     /old_locus_tag="SOI72_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005065049.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGFAPA"
                     /name="repM"
     gene            195..725
                     /locus_tag="SOI72_RS18725"
                     /old_locus_tag="SOI72_18725"
     CDS             195..725
                     /locus_tag="SOI72_RS18725"
                     /old_locus_tag="SOI72_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005065050.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSQNKIDLAEAIRV
                     FGEPVKKQDVKEPVKIDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPNMTTFTDQELVQPSIAKPDPEPDTAEAKHDGLTTPERRENKWIPVPEHIEPEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            1075..1392
                     /locus_tag="SOI72_RS18730"
                     /old_locus_tag="SOI72_18730"
     CDS             1075..1392
                     /locus_tag="SOI72_RS18730"
                     /old_locus_tag="SOI72_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000900860.1"
                     /translation="MLGKNHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            1913..2257
                     /locus_tag="SOI72_RS18735"
                     /old_locus_tag="SOI72_18735"
     CDS             1913..2257
                     /locus_tag="SOI72_RS18735"
                     /old_locus_tag="SOI72_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_005065055.1"
                     /translation="MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSGKKRFYTEMLAIADKQ
                     YELHLSTLGE"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2262..2564
                     /locus_tag="SOI72_RS18740"
                     /old_locus_tag="SOI72_18740"
     CDS             2262..2564
                     /locus_tag="SOI72_RS18740"
                     /old_locus_tag="SOI72_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_005065057.1"
                     /translation="MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            2656..3057
                     /locus_tag="SOI72_RS18745"
                     /old_locus_tag="SOI72_18745"
     CDS             2656..3057
                     /locus_tag="SOI72_RS18745"
                     /old_locus_tag="SOI72_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065059.1"
                     /translation="MTDKKQNDHLNLDGINSSYNDGDGLRINNPEDFRSITISNGYFSN
                     NKGNGITIGSPQQSPLEIILTQLAPKLPDTIQPYELASVIQNLLESTNQEEISQKLMTS
                     GLKEKFKDPNLWISFSSLLFSLIFQFSSK"
                     /name="hypothetical protein"
     gene            3109..3783
                     /locus_tag="SOI72_RS18750"
                     /old_locus_tag="SOI72_18750"
     CDS             3109..3783
                     /locus_tag="SOI72_RS18750"
                     /old_locus_tag="SOI72_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_005065062.1"
                     /translation="MSQKILQRYEQLLTDSASIKAMTKKAYSEYSGSYDTLGDEGDALY
                     LEWKVKVKNLLLLSCGEHSIHYRDFLDAEETQSFDTNTRIISRLIPILKASYDDFKNGF
                     LTSFKQIVQAEVFDSELEQARSLLSSGYKNSAAVIAGVVLETAIKELCLNNGIELEKKK
                     LTRLNEDLAKAGVYNPLQQKLITAMADIRNNAAHGDYDQFTKEDVHRMIEDIERFLLAY
                     SS"
                     /name="HEPN domain-containing protein"
     gene            complement(3874..4185)
                     /locus_tag="SOI72_RS18755"
                     /old_locus_tag="SOI72_18755"
     CDS             complement(3874..4185)
                     /locus_tag="SOI72_RS18755"
                     /old_locus_tag="SOI72_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005065064.1"
                     /translation="MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEI
                     TDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQGKGYQTRMNKILREAMLEDLKNH"
                     /name="BrnA antitoxin family protein"
     gene            complement(4172..4459)
                     /locus_tag="SOI72_RS18760"
                     /old_locus_tag="SOI72_18760"
     CDS             complement(4172..4459)
                     /locus_tag="SOI72_RS18760"
                     /old_locus_tag="SOI72_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002124950.1"
                     /translation="MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIISARQVTKAERNQYEHG"
                     /name="BrnT family toxin"
     gene            4972..6459
                     /locus_tag="SOI72_RS18765"
                     /old_locus_tag="SOI72_18765"
     CDS             4972..6459
                     /locus_tag="SOI72_RS18765"
                     /old_locus_tag="SOI72_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953322.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005065066.1"
                     /translation="MLSTIREQWFSNIRGDVLAGIVVALALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVIAFVGGRPGMISGATGAMALLMVTLVKEHGLQYLLAATILTGFIQILAG
                     YLKLGALMRFVSKSVVIGFVNALAILIFMAQLPELTNVTWHVYAMTIGGLAIIYLFPYI
                     PVIGKLLPSPLICIVLLTLFALFIGLDVRTVGDMGQLPDTLPIFLLPDIPLNLETLTII
                     LPYSLGLAAVGLLESMMTATIVDDLTDTNSDKNRECKGQGVANIASGFLGGMAGCAMIG
                     QSIINVKSGGGTRLSTFSAGVFLLIMVVFISDWLKAIPMAALVAVMIMVAIGTFNWDSL
                     RNIRQYPLSSNIVMIVTVIVVVATHNLAYGVLVGVLLSALFFANKIERYMAIQSEFNEP
                     ENTRTYTVTGQVFFSSADKFTSAFDFKEALSKVVIDVSQAHFWDVSAVAAVDKVVIKFR
                     REGTEVELIGMNEASKTLVDKFGIHDKPDALDRLNSH"
                     /name="SulP family inorganic anion transporter"
     gene            6472..7323
                     /locus_tag="SOI72_RS18770"
                     /old_locus_tag="SOI72_18770"
     CDS             6472..7323
                     /locus_tag="SOI72_RS18770"
                     /old_locus_tag="SOI72_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034565.1"
                     /GO_function="GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_005065068.1"
                     /translation="MSKIIACIDGSLATNTVCDYAAWFSDKLSSPLKLLHVIDKPKAKA
                     PQDLSGAIGLGSRETLLKELVEIEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSLLETIMGMEEDIRVLVMGKHGAETEHDSSKVGTHIENVVRALHKPVLITSSPFSPP
                     KSFLIAFDGSPTARICVDKIANSPLLKTLTAHVVYVGKPNSDMTLQVTWAKELLANNGF
                     DVIDSMLDGDVEKSILDYQEKNVIDMIVIGAYGHSKIRQFFIGSTTTKLLSSSTKPVLL
                     LR"
                     /name="universal stress protein"
     gene            complement(7594..8478)
                     /locus_tag="SOI72_RS18775"
                     /old_locus_tag="SOI72_18775"
     CDS             complement(7594..8478)
                     /locus_tag="SOI72_RS18775"
                     /old_locus_tag="SOI72_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_005065070.1"
                     /translation="MFYLNLKHNIKKEQSSLNAFHYLTRTAHFENQKDNEKLEFMRYGN
                     MPKWAENKPKLFWKSADQFEISRGRTSSTLTIALPKELILEQRAELVQKLIDQFAGQYQ
                     FPYTAVIHNHPSEITGEDQPHLHLMYSERTISDDIERPPEQFFKQYRPKNPTQGGAQKL
                     TADALGFGRNQIKVFREKTQELMNLFLEKYAPTKKVMVYGVEIEVKNQVSCLSNDDFNQ
                     KFGTKLQDVPQIPRWKLYSADPIIQLDVEAQKNTIKKIRNQNNAELYGKEYDLEISRRH
                     VLTQKKDPGFSLD"
                     /name="MobA/MobL family protein"
     gene            8934..9647
                     /locus_tag="SOI72_RS18780"
                     /old_locus_tag="SOI72_18780"
     CDS             8934..9647
                     /locus_tag="SOI72_RS18780"
                     /old_locus_tag="SOI72_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065071.1"
                     /translation="MAGRKTFALTQQEIIDLDKHIRALPFKRRMQDAFLAMISERESMK
                     QLNEEDLKLIQRCRYERNTYNKRIVQLQLIQKTEPQKLKKLELNILELSKKTDIDAYFA
                     MHDALNEVLKIKRNQTSEKNITAKVERVLNPEKYNQAPLSKSLRKQQDHIKYFIGSLYL
                     NLLEQSGLERSNSNEDLDRMLDLFKQAQSGAEKNNAFSLDQNRKTDILSAKSLYEMEKL
                     IKQAKEDPRNPFNKA"
                     /name="hypothetical protein"
     Typing          10346..11147
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.392"
                     /coverage="86.0"
                     /evalue="0.0"
     Typing          complement(7598..8478)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaggcaccga aagcttccaa caatttgcta tacgcattgc agaaatgctc caagattatc
       61 aaaaattcca agaattattc ccttatcttc aaaaagttgg ctttgcacct gcctaaaatt
      121 taaacttgat catttgttta ttagacagtc taatagacaa caacttttga caggtttagg
      181 catagattta caacatgaaa agccttacag ttctcgagct ttctaagctt tacaatatca
      241 atagacaaac gatttacaac aacataaaga aaggaatttt aagtaaaaat tcccagaata
      301 agatcgatct agccgaggct atccgtgttt tcggggaacc tgtcaaaaaa caggatgtaa
      361 aagaacctgt aaaaatagac agtccaaatt cggcagaagt tttactactt agacagcaga
      421 tagacatgct gaaaaaccag cttgatgatg ccaaagatcg ggaatctttc tatcagaacc
      481 aaattgaaac gatgcaacgc ctattagaag ccccaaaacc taatatgact acgtttaccg
      541 atcaagaatt agtgcaaccc agcatagcca aaccagatcc cgaacctgac acagcagaag
      601 ctaaacatga cggattgact actccagagc ggagggaaaa taagtggatt ccagtgccag
      661 agcacattga accagagcct gagaaaagag gcttttggag ccgttttttt agaccctatg
      721 attaaccacg attgtttaat taatatgaat aaagtcgtga aactttgatt aaataataag
      781 cgtggaacgt gaaaaatcat aaaaaaaaag cccaccttgg ggaggttggg caaaaaagaa
      841 actacagcgt tgatttctaa ggaaaattat aacacatttc gtataaggtg tattatgtta
      901 ataatataaa tttttaaaag catattgatt taataataga aaaattatca gagtagggca
      961 tgtgtaaagt aaacgttcga ttattaacca ataatgatta aagatttata tcttgtatgt
     1021 gttaatttta ttaacatata tatacgaaat taataatgcc ttaagtgaaa tagtatgcta
     1081 ggtaaaaatc atcatttttt aaaaaaattt tcgatcagtg atgctgctat agcgtataaa
     1141 aaacctcgct cgactatact taaatatata aaaagcggag atttcgaact tgactacaat
     1201 aaattgattg aatacagtca gttagttaga aaatttggtg aacttactta ccctatacag
     1261 gacgaaagag agcaacaaat caaaaaacta aaagatgaag ttatgcattt aaaaaaacaa
     1321 cttaactttt ttaaatatac ttcaatctca aacaaaaata gactattaat ttctcataaa
     1381 cattccattt aacataatag agcttatacg aaatgctttt taaaatctcc ttgtttttca
     1441 tatagttaaa aatttttcat ccccctaaaa aaccacaaaa aaccgactgg gaaacaagtc
     1501 ggtttctttt taagcaaaac tgtaactttt agccaataaa atcgatcaaa aaacattcgt
     1561 ttttcatttt ttcctgattt ctcaatggtc acacttcaca aataatcccc aatttctgcg
     1621 gacaactctt gggataaaat aggccaactt tgtaagctaa agcttacaga gatttaagga
     1681 ttttgctgat tggtaatcca aggtactagc cctattatga atacataagg gaactggatg
     1741 tctccagaca acaagaataa gaaaatagcg caaggaatgg gcagcacgag atcagtaaga
     1801 cgagcacccg atatgaaaaa gcccgacagg atgtcgggct tttttattgc catttgcaaa
     1861 gttattcaat cagctttaat atatagacta taatctatat tatggttgta atatgtggac
     1921 agtcattaca acggatctgt ttaatgaatg gcttgaacag caagatgaaa taacacaaga
     1981 gaaagtctta gctgcgttgg ttgttctgca actgcaagga ccaagtttag gtcgtccctt
     2041 agtagatacc gtctacgatt ctaaatttac caatatgaag gaactacgtg ttcagcatcg
     2101 aggtaaacca ttaagagcat tttttgcatt cgatccatta cgacaagcaa ttgtactgtg
     2161 tattggtgat aagagcggta aaaagcgttt ttatacagag atgctagcaa ttgcagataa
     2221 gcaatatgaa cttcatctct caacattagg agaataatct gatggctaag accctacaag
     2281 aattattggc taatcgctct cctgagagcc aagcccgtat tcaaaaaatg gctgatgaat
     2341 tgctattaga aaatcagctt taccttattc gtgaagaact cgaaatctct caaaaagaac
     2401 tggctgaaac tttgggaata aaacagccat cgctttcagc aatagaaaat cgtggcaatg
     2461 atcttaagat ttcaaccatg aaaaagtatg ttgaagctat gggaggaaag ctccgtattg
     2521 atgtggaact tccaactggg aaacatatag gattcaatgt atagatatag aaaagtgagt
     2581 ttaagctcac ttttttcatt tggtctgatc agtatgatat aagttataaa tcattcaaaa
     2641 gataaaggct tatatatgac tgataaaaaa cagaacgatc atctaaatct tgatggaatc
     2701 aactcatctt ataatgatgg tgatgggcta agaataaata atcctgagga tttcagaagt
     2761 attaccatat ctaatggcta tttctcaaac aataaaggga atggaattac tattggctca
     2821 ccgcaacaat caccattaga aatcattctt actcaattag ccccaaaact tccagatact
     2881 attcagcctt atgaattggc atcagttatt caaaaccttc tggaatcaac taatcaagaa
     2941 gaaatatctc aaaaattaat gacttcaggc cttaaagaaa aatttaaaga tcctaatctt
     3001 tggatatctt tcagtagttt attgttttca ttaattttcc aattttcatc taaatagatt
     3061 tatagagttc aattacttaa tttggaaacc ctaaaaaact gagtaaacat gagtcaaaaa
     3121 atattacagc gttatgaaca actactaacc gattctgctt ccatcaaagc tatgacaaaa
     3181 aaagcttact cagaatatag cggtagctat gatactttgg gtgatgaggg ggatgctctt
     3241 tatcttgaat ggaaagttaa agttaaaaat ctattattac tatcatgtgg tgaacattct
     3301 attcattacc gtgatttttt agatgctgaa gaaacacaga gctttgatac taatactaga
     3361 ataatcagta ggttaattcc aattttaaaa gcatcgtatg atgattttaa aaatggtttt
     3421 ttaacttctt tcaagcaaat tgttcaagct gaagtttttg atagtgaatt agaacaagca
     3481 cgatcgctgt taagtagtgg ttataaaaat tcagctgctg taattgctgg tgtagttcta
     3541 gaaaccgcaa ttaaagaact atgtttgaat aatggaatag aattagagaa gaaaaaatta
     3601 acacgattga atgaagactt ggcaaaagcg ggagtttata accctctaca gcaaaaacta
     3661 attacagcta tggctgatat tcgaaataat gcggcccatg gagattatga tcagtttaca
     3721 aaagaagatg ttcacaggat gatcgaagat atcgaaagat ttctactagc ttactcttcg
     3781 taagactgtc agatttacat agaaaaaatt taattattta acataaaatc tcttatgcga
     3841 agcaaaaatg ggagtctata gctcccattt tcatcaatga ttcttaagat cttctagcat
     3901 tgcttcacgc agaattttat tcatgcgtgt ctgatagcct ttgccttgag cctttaacca
     3961 tgcaagtaca tctgcatcta gacgaacaga tgtttgttgc ttcactggac gataaaattg
     4021 cccatggcga acagcatgac tccagtcggt aatctcagga atgtcagata gatctaattg
     4081 ttcatctggt acagttcctt ttgcaagcaa gcgtttaatt tcagcatctt gcttgtcact
     4141 gaatttttca ttcagttctt tgcgtgtata tctaaccatg ctcatattga ttgcgttccg
     4201 ctttagtgac ttgccttgca ctaatgattc gtatgatctc acagtcatca tcgtcaatga
     4261 ttgtatgagc caccaataac atcagtacac ctttaaccat accgatcgtt tgccaacgtt
     4321 cttcaccatt ggtatgtcga tcctggattg agatccgtaa aggatcttca aaaacgaggc
     4381 ttgccgtttc aaaagataca tcatgttttt tctgattctt ttggttttta gcctcatccc
     4441 attcgaaata cagttccata aaaaacattc aaatttgtat atacaataat gtatcacaaa
     4501 tttgtatcac tcaagctaat tggagtaatt tttagtcaaa attattggca agaagtatca
     4561 aacttgctca aaaaaaatcc gctttgtttc caaagcggat ttttgcggtg gttacgggtg
     4621 atgtaaaata gttaagcata tgattttatt taacttataa aagacgattt cggataacgc
     4681 ccattatgtt aaatgggcat aaatcaattc taaaattatc tatgataatt cttagctttt
     4741 acttcagtac tgcgtttagg gtggatagaa aaggggctag ctgctatgat tgcccccgaa
     4801 atcctccaaa atagatatct ttcttaatat gacacttacg ccaccataag tttatttacc
     4861 tcttaaatca gtaaataact catcactgtc tagtgatgtg gtgtagtagc ttgtgtgtcg
     4921 tatatatcac atcactagcc tttcttaaaa tttaaaaaaa taggctattt catgttatcc
     4981 actattcgag aacaatggtt ctctaatatt cgcggggatg tccttgcggg tattgttgtc
     5041 gctttggctc taattcctga agcaattgcc ttctctatta ttgcaggtgt cgatcctaaa
     5101 gtcgggttat acgcttcatt ctgtattgcc gtggtgattg ctttcgtagg tggtcgccct
     5161 ggtatgattt ctggggcaac cggtgccatg gcgttattaa tggtaacact ggttaaagag
     5221 catggtctgc aatacttatt agcagccaca attttgacag gatttattca aatcttagcc
     5281 ggctatctaa aattaggtgc cttgatgcgg tttgtatcca agtcagttgt gattggcttt
     5341 gtgaatgccc tggctattct aatttttatg gctcaattac ctgaactcac taatgtgaca
     5401 tggcatgtct atgcgatgac aattggtggt ttagccatta tttatctatt cccttatatt
     5461 ccagtgatag ggaagctttt accctctcct ctcatctgta ttgtcttact tacgctcttt
     5521 gccttattca ttggtttaga tgtaagaacc gtcggagata tggggcaatt accggatacg
     5581 cttccaattt tcttgcttcc tgatattcct ttaaatcttg aaaccttaac gattattttg
     5641 ccttattcac tgggattagc agccgtaggt ttgcttgaat ctatgatgac agcaacgatt
     5701 gtcgatgatt taacagatac caatagtgat aaaaaccgtg agtgtaaagg acaaggcgtt
     5761 gccaatattg cttcaggttt cttaggcgga atggcaggct gtgccatgat tggtcaatcg
     5821 attattaatg tgaaatctgg tgggggtacc cgtttatcta catttagtgc aggggtcttt
     5881 ctgttaatta tggtggtgtt tatcagtgac tggcttaaag ccattccaat ggctgcactg
     5941 gttgctgtca tgatcatggt agcaattgga acatttaact gggattcact acgcaatatc
     6001 cgccaatatc ccttatctag caatattgtg atgatcgtta cagtcatcgt ggtcgttgcg
     6061 acacataatt tggcttatgg cgtattagtt ggtgtacttc tttctgcatt attttttgcc
     6121 aacaaaattg aacgctacat ggcaattcag tccgaattta atgaacctga aaatacccgt
     6181 acttatacgg taacaggtca agtgttcttc agctcagcag ataaatttac ttctgcattt
     6241 gatttcaaag aagcactttc aaaagttgta attgatgtta gtcaggctca cttttgggat
     6301 gtttctgcgg tcgctgccgt agataaagtg gtcattaagt ttagacgtga aggaacagaa
     6361 gtcgaactta ttggaatgaa tgaagccagc aaaaccttag tcgataaatt tggcatccat
     6421 gataaaccag atgcattaga tcgcttaaat agccactaag agggatgaaa aatgtcaaaa
     6481 attattgcat gtattgatgg atcattagca acaaataccg tttgtgatta tgcagcttgg
     6541 tttagcgata aacttagttc accgcttaag ttattacatg ttattgataa gccaaaagcg
     6601 aaagcaccac aagatttaag cggtgcaatc ggcttgggaa gtcgagaaac cttgctcaaa
     6661 gaattggttg aaattgaaga gcgtaagggg aagattgaac tagagcatgg ccaaattctt
     6721 cttcgggaag caaagaatta tctattggag aagttctcta ttgatgctca aagttttcaa
     6781 cgccacggca gtcttttaga gacaatcatg ggaatggaag aggatattcg tgttcttgtt
     6841 atgggtaaac atggggctga aaccgagcat gattccagta aagttggaac ccatattgaa
     6901 aatgttgttc gtgcacttca taaacccgta ttaataacat cttcaccctt tagtccacct
     6961 aagtcttttt taattgcttt tgatggaagt cctactgcac gtatttgtgt agacaaaatt
     7021 gcaaatagcc ctttattaaa aaccctgaca gctcatgtgg tatatgttgg taagccaaat
     7081 agtgatatga cattacaagt cacttgggca aaagagctac ttgcaaataa tggttttgat
     7141 gtaattgata gcatgcttga cggtgatgtt gagaaatcta tattggatta tcaagaaaaa
     7201 aacgttattg atatgattgt aattggtgca tatggtcatt caaagatacg ccagtttttt
     7261 attggtagta cgacgaccaa attgttatca agctctacaa aaccagtatt attattaaga
     7321 taatttttaa aagataaaag gatagttttt aactatccct tttttagatt ttctaaaatt
     7381 aacataatac accttatgcg aaaaacctag attttacatt tatgggcaaa aatctaggtt
     7441 ttccttattg acctgaatca aaaacacaat ctatccatca ttgcagccta aaaaacacaa
     7501 attttccatg ttgtggctaa aaatcactca caatatggat aagttttttc aagattccat
     7561 gttcaaaaaa cacaaatttt ccacttataa aaatcaatct aagctaaagc caggatcttt
     7621 tttctgagta agcacatggc gtctagatat ttctaaatca tattccttgc cataaagttc
     7681 agcattgttc tgatttctaa tttttttaat tgtgtttttt tgagcttcaa catcaagctg
     7741 aataattgga tcagcgctat aaagtttcca acgagggatt tgtggtacat cctgtaattt
     7801 ggtcccgaat ttttgattga aatcgtcatt agataggcaa ctgacctgat ttttcacttc
     7861 aatttctaca ccataaacca ttactttttt agttggtgca tatttttcta aaaacaaatt
     7921 cattaattct tgagtttttt cacggaatac tttgatttga tttcgcccaa aacctaatgc
     7981 atcagctgtt aacttttgcg caccaccttg agttggattt tttggccggt actgtttaaa
     8041 aaactgctct ggtggtcgtt caatatcatc tgaaattgtt ctttctgaat acatcaaatg
     8101 taaatgtggt tgatcctctc cagtaatttc actgggatga ttatgaatga ctgcggtata
     8161 aggaaattga tattgtccgg caaactgatc tattaacttc tgaaccaatt ctgctcgttg
     8221 ttctagtatt aactcttttg gtaaagcaat ggttaaagta gagcttgttc ttccccggga
     8281 aatttcaaat tgatcagcac ttttccaaaa aagttttggt ttattttctg cccatttggg
     8341 catatttcca taccgcataa actctaattt ttcattgtct ttttgatttt caaaatgtgc
     8401 cgtccgagtt aaataatgga atgcattcag agaactctgc tcttttttaa tattatgctt
     8461 tagatttaaa taaaacatca gttcacctca aacaaattac gtgtaacgaa acagcaaatt
     8521 ttcttgtaac aaggggctaa ttcttgttgc agagagcagt ctcttcttgt tgcaaacatc
     8581 gatatttcat caaaaaagtt tgttgcactc gtctaaaatt cgctgttttc cttgttgcac
     8641 ccctttccca tttcttgtaa cggcagacat tgcttacttt ttcaccatta agaaaaatac
     8701 cgttaaaacc cgaagaaaaa ctcataattt aataattaaa tcagtggttt atgtagcctc
     8761 cgcaggagcg agtagaagtg tcttttttta acaatgattg ctaaagcaat cattgttaaa
     8821 aaaagacacg gaaacgagat aaacaatatt ttatgtataa cataaaatat tgtttcaaca
     8881 ccatcagaat tccgctaaca tcaatttaga tacccaaaat caggaaatcc gctttggccg
     8941 gtagaaaaac atttgcctta acacaacaag aaatcatcga tttggataaa catattcgag
     9001 cgctgccgtt taaaagacga atgcaagatg cttttttagc aatgatttct gaacgagaat
     9061 caatgaaaca actgaatgaa gaagatttaa aacttattca acgatgtcgt tatgagcgca
     9121 atacttataa taaacgtata gttcaattac aactgatcca aaaaaccgag ccacagaaat
     9181 taaaaaaatt agaattgaat attctagaat taagtaaaaa aacagatatt gatgcttact
     9241 ttgcaatgca tgatgcatta aatgaagttt taaaaattaa aagaaatcag acttctgaaa
     9301 agaatattac agcgaaggtc gaacgagttt taaaccctga gaaatataac caggcccccc
     9361 ttagtaaatc tttaagaaaa cagcaagacc acattaaata ttttatcgga tcactatatt
     9421 taaacttatt ggaacaaagc ggtttagagc gatcaaattc aaacgaagat ttagatcgaa
     9481 tgctcgattt atttaagcag gcacaatccg gagcagaaaa gaacaatgca tttagcctgg
     9541 atcaaaatag aaaaacagat attttatcgg ctaaatcgct atatgaaatg gagaaattga
     9601 ttaagcaagc caaagaagat ccaaggaatc ctttcaataa agcttagatt gactcaaata
     9661 atagggttta aatacactgt tgaagccgta tatgcctttt ttagcctaaa cctatattcc
     9721 ccatcaataa aaaaattaga gtgctctaaa cgcaaattag agtattttgg agatcatttg
     9781 attttaccga taagaacaaa gcgagtgtct acgagcgatc cttggaaaat tttgcctaga
     9841 ctccctgaaa aaccgctttt taggctgtga aacctaaata agcatggaaa agttgtagaa
     9901 atttgagtct agatcgagcg tagcgagtaa aaaagtttta tgagcgtagc gaattccgag
     9961 ttgcttttgc ttttttaaag tcacgtaagt gctcaccaaa aaattgcccc gacgaactga
    10021 gcgaaagcga agttcaatag agtttgagcg aagcgaaaac caagggcaat ttttctttac
    10081 ctgggctttt aattattttt aatcttttaa atgcttttaa gtaagctgta accctttatt
    10141 cataatagtt ttagagttaa taaaactagg tttatcgacc cataaaacta ggtttatcga
    10201 cccataaaac taggtttatc gacccataaa actaggttta tcgaccttaa aactaggttt
    10261 atcgacccca aattaagtcg atttacaatt caattaaaat agtctattgt acaacctagt
    10321 ttaattataa atttattatt agaaatgagt gaattaatcg taaaggataa tgctttaatt
    10381 caagctagct ataccctaga tacagttgaa caaaggctga tcctattagc cattgctgaa
    10441 gctcgggaaa cagggcacgg gataactgaa aatagccttt tagaagtaca tgcaagtagt
    10501 tatatcaata cctttaatgt cgagaaacac actgcttata ccgtgcttcg ggatgcatct
    10561 aaaagcttgt ttgatcgcta tgtcacgtat catgacatta atcctaagac tggtaaggat
    10621 cgtagctttc actgtcgttg ggtcgataaa attggatatg aacctcaatc cgggatcgtt
    10681 ttcctacgtt tcacgcaaga cattgttcca ctcataactc gactggaaga aaactttact
    10741 aaatatgaat tggagcaggt ttcgagatta actagctctt atgctattcg gttatacgag
    10801 ttattaattc aatggagatc tgctggtaaa acgccaattt ttgatctatc gatctttaga
    10861 caacaacttg gtgtcgaagc tcatcaatac aaaacaatga gtaattttaa aacgtatgtt
    10921 ttagattttg ccctcaagca agtaaatgag ttaaccgata taacggctaa atatgaacaa
    10981 cataaaaaag gacgttcaat ttccggtttt tctttcagtt ttaagcagaa aaaaaccaac
    11041 tctgacaaag taattaaggg aacggataca ttagcccttt tcaccaaaat gagtgataaa
    11101 caacgtcatc tctttgccaa caaactttct gaattacctg agatggggca atattcag
//
