LOCUS       NZ_CP139128             4715 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Escherichia coli strain YZ22PE244 plasmid pYUYZPE244-3, complete
            sequence.
ACCESSION   NZ_CP139128
VERSION     NZ_CP139128.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38245694
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Wang,J.
  TITLE     tet(X4)-positive Escherichia coli strains in Yangzhou
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Wang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2023) Key Laboratory of Prevention and Control of
            Biological Hazard Factors (Animal Origin) for Agrifood Safety and
            Quality, Ministry of Agriculture of China, Yangzhou University,
            No.48 Wenhui East Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.5.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 100x
            Sequencing Technology        :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/24/2024 10:55:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139128.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="YZ22PE244"
                     /isolation_source="Pig farm"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="pYUYZPE244-3"
                     /geo_loc_name="China: Yangzhou"
                     /lat_lon="32.27 N 119.29 E"
                     /collection_date="2022-06-02"
                     /collected_by="Jiang Yue"
     gene            join(4368..4715,1..519)
                     /locus_tag="SIY77_RS00005"
     CDS             join(4368..4715,1..519)
                     /locus_tag="SIY77_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_000409995.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            516..1169
                     /locus_tag="SIY77_RS00010"
     CDS             516..1169
                     /locus_tag="SIY77_RS00010"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_000560060.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="PP2C family serine/threonine-protein phosphatase"
     gene            complement(1391..1504)
                     /locus_tag="SIY77_RS00015"
     CDS             complement(1391..1504)
                     /locus_tag="SIY77_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427893.1"
                     /translation="MHEPPVQPDCCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(2158..2352)
                     /locus_tag="SIY77_RS00020"
     CDS             complement(2158..2352)
                     /locus_tag="SIY77_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3428..4384
                     /locus_tag="SIY77_RS00025"
     CDS             3428..4384
                     /locus_tag="SIY77_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000280763.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     Typing          2003..2085
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.81"
                     /coverage="100.0"
                     /evalue="1.76e-34"
     Typing          2389..2487
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          2612..2867
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.124"
                     /coverage="89.0"
                     /evalue="5.65e-115"
     Typing          complement(1433..1990)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.821"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagataaata tagccttttg cgatcgttct aatgcgtctg caatgaaaag gcttgaatct
       61 aatcaatcac cttcagtgat aaatatcgat tttagcttta acgaaaaaac gtatgatttt
      121 gactttttat cattagatgg tgatgatgac aatgatagcg taaagaccta cagcttaact
      181 gatttgatgg ctgaaaaata tcgatccgtt ttgcaacaaa aagttagaga gagaaatcga
      241 gagcaagata tatatgatat caattatctt ttaggtaaat atgagttttc tcggcaagat
      301 aagtataaaa tactggagtc tcttttaaaa aagagtgaag gtaaacaatt ggataatctc
      361 ttaaacgtta aaggtataag ggatgtagaa attatcgagc gttccagtca gcgatatcag
      421 gatttatcat ccaccgttaa atcattacct ctttttgaag atgcttatga aaaagttgcc
      481 tgtttctatg aaagtcttcc gtgggatata ttcaaatgat agcgttcagt tttagtgata
      541 ttggtaacag atgtgaaaac ttagacttat tatctacgat aaattgtcaa aatgatgtgt
      601 ttggaatggt tattatggat gggtattcct gctctgagtg gaatatgaaa attctctctg
      661 gtaggttaga taagtgcgtg ttttctggtg atctgaataa agatattatt gaaccatgca
      721 agtcagttcc tgttaaggct gcagtatgtt tcctttttaa aaataatacg cacctccata
      781 tatataacgc aggagattgc cgtgtatata acaaaagtgg agtccttcta acagaggata
      841 actcggttgc ctggaaaaga ttaaaggaaa atggaatcga taataaaaaa atacctcacc
      901 tcgtttgtta ttgcccagac agaaattatc taacatcatt tgttgatttc tgctctgatg
      961 agataatttt caatcattca gtttttgatt taaatgatga aaacaaagag gttattttat
     1021 gtactgatgg gttttgggag cattttaatg atggtactgt tattgacctc atgaaagata
     1081 acccagaatc taacttggct aaatttacta aaaatatccc gggtaacgca gagaatcata
     1141 cggtttgcta cgttagattc ccttcttgat ttcgtgtttt ttgaagaagc tattgagatc
     1201 ttcgtgtcat atagataatc tcttttctgt aaaagaaaaa aaccgccttt gcggggcggt
     1261 tttctcttag gttcaaagag tttgcggctc gaagaaccaa ggtaactggc ttggaggagc
     1321 gcagtcacca aaacctgttc tttcagttta gccttaactg gcgcatgact tcaagactaa
     1381 ctcctctaaa tcagttacca gtggctgctg ccagtggcgt taaggcgtgt cttaccgggt
     1441 tggactcaag acgatagtta ccggataagg cgcagcagtc gggctgaacg gggggttcgt
     1501 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccaa cagcgtgagc
     1561 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1621 gggtcggaac aggagagcgc acgagggagc caccaggggg aaacgtctgg tatctttaag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ctgtgatgct cgtcaggggg
     1741 gcggagccta tggaaaaacg cctgcggcgc ggccttcttc ctgttcttgg tcttttgctc
     1801 acatgttctt tccggcctta tcccctgatt ctgtggataa ccgtgctgcc gtttttgtgt
     1861 aagtcagtac cgctcgccgc agccgaacga ccgagcgtag cgagtcagtg agcgaggaag
     1921 cggaaaagcg cctggacgcg cattttctcc ttacgcatct gtgcggaatt tcacacccgg
     1981 catggggcac ttttcataca atctgcattg atgccgcata gttaagccag tatacactcc
     2041 gctatcgcta cgtgactggg tcagggcttc gccccgacac ccgctgacgc gccttggcgg
     2101 gcttgtctgc tgccgaaaac gctcgcggac aagccgtgag cgcctccgtc agcttcgtta
     2161 tgttcctgtt tcactttcgg tctcaaagcg aacctggatg ctgttctgga gttcttccgc
     2221 gagttcgtgc agtcgttcgc acatggccgc ctgttcgtcg gcatccagag catccagttt
     2281 ttcaagcagt gtcaggctct ggcttttgat aaatcgcgcc atattcagcg cagtttgttg
     2341 ctgtttgttc accttccgtt gttctccgtc agtaccgggt gtcgggtgga ccctgaccag
     2401 gtggtaattg taagacgttg cgggcgacgg tattacaatt gcacatcctg tcctgttttt
     2461 ttaggacgta tcatggcaca gcatatcaac tgataaataa tccctgtgca gtgcagccgc
     2521 tgttgcataa aatttcgctc tgcgtcgagt gtttcttaat caataattcc gctgatactc
     2581 cggaaaaacg cgagtctgtt ctcatgcctg aaatgcccac accccacgca aaaacaattt
     2641 tttgctgatt tttctttata aatagagagt tatgacaaat tagttcttct tgctctcttt
     2701 gtgatattta aaaaagcggt gtcggcgcgg tgttgtagct gcgccaacac cgctttttag
     2761 gggtggtact gactattttc ataaaaaaca tcattttata ttaggggtgc tgctagcggc
     2821 gcggtgtgtt tttttacagg acgcccctgg gggcgctgcc aggggtgtct gttcagatat
     2881 gtagcaatga gtcagggatt cacgaactga aaatgtccga aaatgtgtct catgggcacc
     2941 gtatgcgccc aaaccgatcg ccattcactt tcatgcatag ctatgcagtg agctgaaagc
     3001 gatcctgacg catttttccg gttttcgctt ggtaaaacat ctctttttgc ggtgttcgcg
     3061 gcagaatcgc gttcagcgcg ttttagcggt gcgcgtaatg cgacgttatg gtaaatgacg
     3121 ctgaaaaatg atcttttacg ctcatcgttt atgagcaaca gatgtcctaa tcatggattt
     3181 gaactataac cgccgctgaa ttgatcgcat gaggtgttat gaaaacggtg atataagtaa
     3241 actttttctt gacttcctac ttattatcct ataaaataaa tcctaaggat ttattttata
     3301 ggatatcaaa aatataaaaa tggcaaatgc ttgtttgtta ttattttttt cttgacaatc
     3361 aatctctaat agaatatacg tccttaggac gtatattcta ttagagacta aaccgaaaag
     3421 gttgaatatg tatcttatcg aagaagcgct aacgtcttgg ttgttgaatg aatataaaaa
     3481 accagtcata accaaagaag aaatctcttt gcagatttac cttttttatt taaaaaaaga
     3541 atataatggt gagaggatat atcaaattag aaagaacaaa gcaggaccta atgaatttga
     3601 aaaaaatata ttaaaattat taaagtcagg ggttttatca agagttgaaa gtaacgataa
     3661 gcatcatgct tacccccatt taattggcgg tttctatgag gtcttttttg ttaaaggtaa
     3721 cgatggttct ccggaaaatg atatagtgtg ctctacgtat ccatatggat acttatccta
     3781 tttatccgct atggattggc ataacatcac agataaaata cccaagataa tccactttac
     3841 gacatgtcgt tccgaacagt ggaagaataa gttctatcat gattttcaaa aggataatcc
     3901 tgatatagac gaagccaaac aattcatgcc tagatttcca agaaattctg aggtcaatgg
     3961 aataaaaatt atcgttacac aagaaacaaa ttatgttgag ccaatgaagg ctcgtaattc
     4021 ccctgtcagg gtatcatcta tcggtaagac ctttatggat atgaccaggc ggcctgatta
     4081 ctgtggtggt gaggagcatg tattggaatc atttattgag cacggtaaaa aatatgcacc
     4141 tctgattata aaggaagtcg ataagcttgg ccgtcatatt gacaaggtaa gggttggatt
     4201 cattttggat agaattattg gggttaataa taaaaaaata agcgaatgga aacaagaggc
     4261 gggtaaatac agaggtagtt cgaaagtgct atctccaaag agtccattct caccttactt
     4321 tgatgaagaa tggtttattt ctattaatgt tgatatggtt aagtcttatg gaaaaagaaa
     4381 ttaataattg gatcgaagag tctggtggga agtacaggtt gatgcgccag gctgtacata
     4441 taatacttaa atctatttct atagaggaga gtttgaatga aatgatggtt atgaagggag
     4501 ggactcttct tggtgttaag catggtagtg atagatacac aaaggatatt gatttttcta
     4561 ccgaaaaaaa actttctgaa attgacttgg gggatttttt caataggttt aatgactcaa
     4621 tggatgttgc tgaggctgaa ttgggatatg gtattaaatg taaagttcaa tctcataaaa
     4681 ttcaacctaa tgcacaagga acatttccaa ctgta
//
