LOCUS       NZ_CP139040             8555 bp    DNA     circular CON 28-JAN-2024
DEFINITION  Escherichia coli MP1 plasmid unnamed, complete sequence.
ACCESSION   NZ_CP139040
VERSION     NZ_CP139040.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN01998190
            Assembly: GCF_034265375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli MP1
  ORGANISM  Escherichia coli MP1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Pick,K., Stothard,P. and Raivio,T.L.
  TITLE     Complete genome sequence of Escherichia coli MP1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8555)
  AUTHORS   Pick,K., Stothard,P. and Raivio,T.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) Biological Sciences, Univeristy of Alberta,
            116 St & 85 Ave NW, Edmonton, AB T6G 2R3, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycyler v. v0.5.4; Canu v. v2.2; NextDenovo v.
            v2.5.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 266.0x
            Sequencing Technology             :: Oxford Nanopore; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 21:55:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,613
            CDSs (total)                      :: 4,499
            Genes (coding)                    :: 4,360
            CDSs (with protein)               :: 4,360
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 60 of 139
            Pseudo Genes (incomplete)         :: 89 of 139
            Pseudo Genes (internal stop)      :: 27 of 139
            Pseudo Genes (multiple problems)  :: 35 of 139
            Pseudo Genes (short protein)      :: 3 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Escherichia coli MP1"
                     /mol_type="genomic DNA"
                     /strain="MP1"
                     /db_xref="taxon:1314835"
                     /plasmid="unnamed"
     gene            join(7857..8555,1..1191)
                     /gene="cya"
                     /locus_tag="SID94_RS23015"
                     /old_locus_tag="SID94_23015"
     CDS             join(7857..8555,1..1191)
                     /gene="cya"
                     /locus_tag="SID94_RS23015"
                     /old_locus_tag="SID94_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin Y"
                     /protein_id="WP_106372970.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGITSTQAKQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLLPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKDMGRVTERVVDAVNLALILNQIS
                     SGMSASQKDVDAANRIINETVKAINDVNLKIAEKKKQQVPLLSLMKQKQKEVEELKKVF
                     KNHSYHRIRDAQRAYDDARNKNDLLVSDINALQAQVSGLTARKQQAEKNKAAAEKAKAD
                     AKAKAEAEKAAAEAKAKAEAEKARKEAEAKANNEKAVLTKASEIIISVGDKVGEYLGDK
                     YKALSREIAGNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDVIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMREVESWVVSGIASAVAL
                     AIFSATLGAYLLAVGASAAVVGIIGIIIASFIGALIDDKFIDRLNNEIIRPAY"
                     /name="cya"
     gene            complement(1219..1743)
                     /gene="cui"
                     /locus_tag="SID94_RS23020"
                     /old_locus_tag="SID94_23020"
     CDS             complement(1219..1743)
                     /gene="cui"
                     /locus_tag="SID94_RS23020"
                     /old_locus_tag="SID94_23020"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="WP_021534853.1"
                     /translation="MRKEHTDNKDLNRTLFLITFAGITPLIIIFITYTSNPKFYLISII
                     FDNTQNIPSIISAYNPVMTKVMDIYGKSAPLLALIAFTLQLRDRKLETIANREKLITAS
                     IFSPFFYAFYAYFFLWNNFELTTAGRTVRWMSDNDFTLFIFYACLYFCSFFMTYALCYI
                     PVVSYKLWKER"
                     /name="cui"
     gene            1863..2003
                     /gene="crl"
                     /locus_tag="SID94_RS23025"
                     /old_locus_tag="SID94_23025"
     CDS             1863..2003
                     /gene="crl"
                     /locus_tag="SID94_RS23025"
                     /old_locus_tag="SID94_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_010892547.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSKLTGIAV
                     Q"
                     /name="crl"
     gene            complement(2200..2358)
                     /locus_tag="SID94_RS23030"
                     /old_locus_tag="SID94_23030"
     CDS             complement(2200..2358)
                     /locus_tag="SID94_RS23030"
                     /old_locus_tag="SID94_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(3028..3219)
                     /locus_tag="SID94_RS23035"
                     /old_locus_tag="SID94_23035"
     CDS             complement(3028..3219)
                     /locus_tag="SID94_RS23035"
                     /old_locus_tag="SID94_23035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3284..3607
                     /gene="mobC"
                     /locus_tag="SID94_RS23040"
                     /old_locus_tag="SID94_23040"
     CDS             3284..3607
                     /gene="mobC"
                     /locus_tag="SID94_RS23040"
                     /old_locus_tag="SID94_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032286519.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQMAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="mobC"
     gene            3816..>4247
                     /locus_tag="SID94_RS23045"
                     /old_locus_tag="SID94_23045"
                     /pseudo=""
     CDS             3816..>4247
                     /locus_tag="SID94_RS23045"
                     /old_locus_tag="SID94_23045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4285..4815
                     /locus_tag="SID94_RS23050"
                     /old_locus_tag="SID94_23050"
     CDS             4285..4815
                     /locus_tag="SID94_RS23050"
                     /old_locus_tag="SID94_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_058813529.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTISTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            4822..5055
                     /locus_tag="SID94_RS23055"
                     /old_locus_tag="SID94_23055"
     CDS             4822..5055
                     /locus_tag="SID94_RS23055"
                     /old_locus_tag="SID94_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021534848.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6685..7113)
                     /locus_tag="SID94_RS23060"
                     /old_locus_tag="SID94_23060"
     CDS             complement(6685..7113)
                     /locus_tag="SID94_RS23060"
                     /old_locus_tag="SID94_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021536732.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQYTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(7194..7631)
                     /locus_tag="SID94_RS23065"
                     /old_locus_tag="SID94_23065"
     CDS             complement(7194..7631)
                     /locus_tag="SID94_RS23065"
                     /old_locus_tag="SID94_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536733.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQESEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     Typing          2856..2943
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          complement(2287..2830)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.689"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3598..5138
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.09"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagggagagg ctgtcagaca gattgttgca gcctggagtc tggcatatca ggatttcccg
       61 gtaaatctga aaaaagatat ggggcgagtt actgaacggg tggtggatgc ggttaatttg
      121 gcgctcattc tcaaccagat atcatccgga atgtcggcct cacagaagga tgttgatgcg
      181 gcgaaccgga ttattaatga gaccgtgaaa gccattaacg acgtaaatct taaaatagcg
      241 gagaagaaga aacaacaggt accattatta agccttatga aacaaaaaca gaaagaggtt
      301 gaagaactaa aaaaagtatt taagaaccat tcttatcacc gtattcgtga tgctcagaga
      361 gcatatgatg atgcccggaa caaaaatgat ttactggtca gtgatattaa tgcgctgcag
      421 gcacaggttt ccggactgac tgcaaggaaa cagcaggcag aaaagaacaa ggcggcagca
      481 gaaaaagcaa aagctgatgc aaaggcgaaa gctgaagctg aaaaagcggc tgctgaagca
      541 aaagcaaaag ctgaagctga aaaagccaga aaggaagctg aagcaaaagc aaataacgag
      601 aaagctgttc tgacaaaagc cagtgaaatt atcattagtg tgggtgataa ggtcggagaa
      661 tatcttggcg ataaatataa agctctttct cgtgagatag caggtaatat caaaaacttt
      721 caaggtaaga cgatccgtag ttatgatgag gcgatggctt ctgtcaataa actgatggct
      781 aatcctgatc ttaaaattaa tgctgcagac agggatgtca ttgtgaatgc ctggaaagca
      841 tttgatgcag aggatatggg gaataagttt gccgcgctgg gtaagacatt taaagccgca
      901 gattatgtga tgaaggcaaa taacgtccgg gagaagagta tagagggata tcagaccgga
      961 aactgggggc cgttaatgcg ggaggtcgag tcctgggttg tcagtggtat tgcttctgct
     1021 gtggctcttg ctattttttc tgccaccctt ggagcttacc tgcttgctgt gggggcatca
     1081 gctgcagtcg tagggattat cggtattatt atagccagtt ttattggagc attgattgat
     1141 gacaaattta tagacaggtt aaataacgaa attattcgcc ctgcgtatta acctagagtc
     1201 gaaaggtagt caagaagact acctttcttt ccagagttta tatgacacaa ccggaatata
     1261 acacagagca taagtcataa agaaagagca aaaatagagg caggcgtaaa aaataaacaa
     1321 agtaaaatca ttatcggaca tccatctgac agtcctacct gcagtggtta attcgaaatt
     1381 attccacaga aagaaatatg cataaaaagc ataaaaaaat ggactaaata tgctggcagt
     1441 aattagtttt tctctgttgg ctattgtctc tagctttctg tcgcggagtt ggagagtaaa
     1501 agcaataagt gccagtaagg gagcgctttt cccgtagata tccatcacct ttgtcatcac
     1561 aggattgtat gcggaaatta ttgaaggtat attttgtgtg ttatcaaaaa tgatgctaat
     1621 taaatagaac ttggggtttg atgtataagt tataaaaata atgattaatg gtgttatgcc
     1681 tgcaaaagta atcaaaaaaa gcgtcctgtt taaatctttg ttgtcagtgt gctcttttct
     1741 cattggtata actctccaag cttttcatta ttattaattt agttgtagat gtgtataagc
     1801 atatcatgct gtacaagtaa tggtcatcct aagattaggg gtatgtgtta atgagggaat
     1861 atatgaaaat gatattatca ggagggatgc ttgtcttgat ggctttgcta tcaggctgcc
     1921 aagctaatta catccgcgat gttcagggag ggactgtcgc accatcctcc tcttctaaac
     1981 tgacggggat cgcggttcag tagaaaagtt aaaaggatct tcttgagatc ctttttttct
     2041 gcgcgtaatc tgctgcctgt aaacgaaaaa accgcctggg gaggcggttt gatcgaaggt
     2101 taggtcagtt ggggaactgc ttaaccaagg taactggctt cagcagagcg cagataccaa
     2161 atactgtcct ttcagtgtag ccgtagtcgg gccactactt caagaactct gtagcaccgt
     2221 ttgtgccatc atcgctctgc taatccggtt accagtggct gctgccagtg gcgttaaggc
     2281 gtgccttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacacca agccgagata
     2401 ccaacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc ggcgcggcct ttcgttggcc
     2641 tttgcctttt tgctcacatg ttctttccgg ccttatcccc tgattctgtg gataaccgta
     2701 ttaccgcctt tgagtgcgcc ggtaccgctc gccgcagccg aacgaccgag cgtagcgagt
     2761 cagtgagcga ggaagcggaa gagtgtcgga agttgcattt tctccttacg catctgtgcg
     2821 gcatttcaca tcggctatga tgcacttttc atacaatccg cactgatgtc gcatagttaa
     2881 gccagtatac actccgctat cgctacgtga ctgggtcagg gctgcgcccc gacacccgct
     2941 aaaacctgct gacgcgccct gacgggcttg tctgctctcg gcatccattc acagaccagt
     3001 tgtgtacgtc tccgggagct gcatgtgtca gtggttttca ccgtcatccc cgaagcgtgc
     3061 gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc gattcacaga tatctgcctg
     3121 ttcatccgcg tccagttcgt tgagcttctc cagcagcgtt aatgtctggc ttctgataaa
     3181 gcgggccatg ttaagggcgg ttttttcctg ttttgtcact gatgcctccg tgtaaggggg
     3241 atttctgttc atgggggtaa tgataccgat gaaacgcgag aggatgctca cgatacgggt
     3301 tactgatgat gaacatgccc ggttactgga acgctgtgag ggtaaacaaa tggcggtatg
     3361 gatgcggcgg gtctgcctgg gtgagccggt tgcccgttcc gggaagctgc cgacactggc
     3421 accgccgtta ctgcgtcagc tggccgccat cgggaataac ctgaatcaga cagcgaggaa
     3481 ggtgaacagc gggcagtggt cttccggtga ccgggttcag gtggtggccg cactgatggc
     3541 catcggggat gagctgcgcc ggctgcgtct ggccgtcagg gaacagggga cgcgggatga
     3601 tagttaagtt tcatgccagg ggaaaaagtg gtggcagtgg tccggttgat tacctgctgg
     3661 gaagggagcg taaccgggaa ggggcaacgg tgcttcaggg taatccggaa gaagtccggg
     3721 aactcatcga tgccacgcca tttgcgaaga aatacacgtc aggtgttctg tcgttcgcgg
     3781 agaaggagct gccgccggga ggacgtgaaa aagtgatggc gagctttgag cgtgtactga
     3841 tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt ggagcaccag gacaagggac
     3901 ggcttgagct gaattttgtc attccgaaca tggagctaca gagcggaaaa cgcctccagc
     3961 cgtactacga ccgggcagac aggccgagaa ttgatgcctg gcagacgctg gttaatcacc
     4021 attacgggct gcatgacccc aacgccccgg agaaccgccg gatactgact ctccctgata
     4081 acctgccgga aacgaaacag gcgcttgcag agagcgtcac acgcgggata gacgctcttt
     4141 atcatgtcgg ggagataaaa ggccgtcagg atgtgattca ggcgctcacg gaggcaggga
     4201 ttgaagtggt cagggtgacg cgaagcagta tcagcattgc agacccgaac ggagggaaga
     4261 atatcagact gaaaggagcg ttttatgagc aatcttttac agacgggcgc ggagttcgag
     4321 aaaaagctga aagagagagc cgaatctaca gagaaaatgc tgaacgacga gttcaggaag
     4381 ctcgaagaat ctgtaaacag ggaactgacc tcaaacgaat ctttaatcag gaacgctatc
     4441 aacgaccata caacagccct gaaagaactg ctggagagat atcagaaaac gaccatcagc
     4501 acgatggata cacactggca aaccgtactg aaaatgagcg tgaaacgctg gctgtggctg
     4561 attatcgtct cagtgctgat gtttgccacg accgggagtc tgctgtggta tcagggcatg
     4621 aagataaatg caaacatgaa tattctccgc gagcagaaag agagtctgga gaagctgaac
     4681 gcgaagacat ggggcgtgcg ttatcacgag gacagcaacg ggcgttttct tgtgttgccg
     4741 aaggggatga aagccgaaac gaactggacg gaggacaacg gcaaactgaa tgcagtgaga
     4801 ctggtacagg agtgacagag catgacggag ctggaaaaac atttgctgaa cgcattagag
     4861 caactgcaac aggactatat gcagcggctg aacgaatggg agagcgcctt cgtggaattg
     4921 cagaaaatgt tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg ggtcatgaag
     4981 ttgagtcagc aggtgcagca cttgagcgag cggacagagc gcttgagcca gttatacagc
     5041 gagaacagga gataagggac gaacggctga tgcaggaacg cgaacatgag ttatctctgg
     5101 aacgggagcg gaagctggat ggcccttcgc tgggatggtg actgaaagtc cggttgagtc
     5161 gtaaacagaa tattttccag aaatgtcagg ctgcctattg gtaatataac cagtcgttct
     5221 tgcggtttat tgatttttgt acaggttgtt gctgtttttt ttgtaaagag gacaggtatt
     5281 tttctgaaaa tgaacttgcg ggaatgcgcg ggttatgtaa aaaatataat tgcgtatctc
     5341 cgatatgttt gtaataccga tgtggacaca gcgaatctgg aaggggtcgg agatgcgcgt
     5401 tctctcatgt gttaatattg agttcttaca tgtggttgca tatttttttt tgcatatttg
     5461 aagtctgcga attatattct gatatgcaaa agtcaggact agacatacta tccatcgatt
     5521 cagtccttac cttacgccct cactgatttc ctgccgtgag ggcttttttc atgggtgtga
     5581 aaaaaggaag ccccacctga cggaggggct gagtgataca gagcaaaaaa atatcctggt
     5641 gttggtcacc tctggagagt aataagatta accataaaac aaactataaa caagaatatt
     5701 gatgccagga attttcctgg ttattttttt ttgtttttta caaaaaagac aaaataacag
     5761 aaagtaaaat ctgatcattt ttgttgattg ttattgctct gtaccgttat tcttaagctg
     5821 aaagtgaatt cttctaattt tttcttatat tcagacgcaa ttaacagtcc ctgctccttg
     5881 agggactttt tttgtctttt tctctctgtt ttatattgtt ttgagtagta actataaagg
     5941 cagtgaagtt ctgtcttatg agcgcgtttc tggcggtgat gcgactaacc cattctacat
     6001 caacgaacaa ttcaccagaa acgcgcctgt aaatttttag ctgagcaagg gctctgtgtc
     6061 gagagttgtg gatgtctggt tcgccatttt aaaaaacttc ttccggcggt ttttagtttc
     6121 gatgcaggtc agggggcgct gtaaagttat gttccctgat tactgtgcca gaatcctgag
     6181 acagacagga ggtctaccgg ttatttttcg aattgggcgc ttccggaaga gagagtttca
     6241 ttgggcgttg gtcgcggatg gtgagcacgt ttcgatgttg tgtgacgaac attcagctca
     6301 gttgatggag actgtcagaa acgcgctcag aagtgttgta acgcgatttc tggtatcagg
     6361 caattgtttc ctttttctgg agcatgatac ctgtgtgtac ggtgatgagt tctgtcacgt
     6421 acagtaatat ttccctcctg atgcttccat ctggcaggag ttgaacctgc cgaaaatatg
     6481 aaaaaacccc cgtttcagtt acatcaggca ggcaattcgt tcgtgattcg catggctgca
     6541 tagatatgca tgaggttaaa atttatcagg tgcgatcgcg gcggtttttc gggtggtttg
     6601 ttgtcatttt tacctgtctg ctgccgtgat cgcgctgaac gcatttcagt ggtgcgtaca
     6661 attaagggat tatggtaaat ccatttactg tcttccctcg tagccatcga gataaaccgc
     6721 agaaatcgtg tcagccagta gcttcatttt ttccggtgat ggcatatcac tgttcgtata
     6781 ttttctgccc gcaaaggtgg ttgtgcttct gagagactga agaaattttt ctgagtggaa
     6841 ttcttttatg cgttgctgtg ccgtttctgc tgatactccg gctgctttgt ctttctcccc
     6901 catctccctg aactgctgaa agaacggaat catcatccgt gtggtgtcgg ccttgttggt
     6961 gtagaagtta gggtcagaac ggtcatcgga agcgtacaca taatgtcggg tatattgttc
     7021 cacactgcct ttcggggtac aggccgtgag ggctgcagcc acagttatca gggatataag
     7081 atacagatag cgtgtgccac cagaacgttt catacgtgtc tccttacctt aaagtattta
     7141 ttctcggtga gttattatcc gaatactatc ttatcatcag cctctgaccg agttcagtga
     7201 ccgaagagtc tggaccagaa tccccgtttc ttttcctgtt ccagtgcctg cctgagctgg
     7261 gctatctcat tctgtaactg ttcccgttct gattcctggt gcctgcgatc catatcctgt
     7321 gcctgtttat cctcaagcat cagcgtcagg cattgtttca gctcattcag ttcccggaga
     7381 atgcgttctg tctgctgatc atgtggattt tctgcctgtc ccacagtgtg cctttcttgt
     7441 gtctcatttt gctttaattc gccgtatgcc cggatcagtt cactggtttc aaactccctt
     7501 cgtccgtcct ggctggtacg gtaactgaca agacctgagc gcatatcacg gtaaaactgt
     7561 gaacgcgatc ttccagtcat tttcagtgcg tcccggagtg tgtgccatgc cataaagtta
     7621 cagtgtccca taggtgtctc atctcatggt ttcagtaaaa cataatgagg tctgagaacg
     7681 gtaatgtttg cgccggtttt tgtggtatcc ggcgggaata gcgcgtggtg tgagagactg
     7741 tttttttgat cgttttcaca aaaatagaag tccacagtct tgacagggaa aatgcagcgg
     7801 cgtagctttt gtactgtaca taaaaccagt ggttatgtat acagtatttg tttttaatgg
     7861 tttgttttaa aagtcaaaga ggaatgaata tgccggggtt taattacggt gggtatggtg
     7921 acggtactgg ctggagttct gaaagtggtg gtccagctcc aggcggtggt atgcatggaa
     7981 atagtggagg ccagcgcgga gataacgcaa acagtagcaa ttctgtgagt cagcagattt
     8041 ctgccattca gaatgatcag aaacttaaac agaaagtggt gaatatgctg attgctgcga
     8101 gaaaaatgaa tcctgaagca aaaatgattc tgggaagtat tgctccttct ggtgttatgc
     8161 aggtcaccat tgaggggata acctccactc aggccaagca gcttggtttg ggagggctgg
     8221 tcatgggata taacgcttct ggtgttattg gggctgtggg cgaaatagac accggtcacc
     8281 gccttaatgc atcaggagca agtactcccg gaagcgaaac ttctgtggag agttttgtga
     8341 acggacagaa acccgccgga gaatggcatg ctgtggcgaa ggacagctgg acaggggctg
     8401 gtccagtgaa tgtagggctg gttaataacg ccatcaaaag tgtccggatt atcaaaaaag
     8461 gctatgtgac tggtgttctc ttgcctgaag aggtgatgaa taaagcagag tacaaagcaa
     8521 tgcggcaggc tttcgattcc cttccactgg caaaa
//
