LOCUS       NZ_CP139033             8812 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella aerogenes strain KA04 plasmid pKA04-6, complete sequence.
ACCESSION   NZ_CP139033
VERSION     NZ_CP139033.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38257105
            Assembly: GCF_034044195.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   fang,y., wu,z. and hu,l.
  TITLE     First Report of OXA-181-Producing Klebsiella aerogenes in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   fang,y., wu,z. and hu,l.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) Department of Clinical Laboratory,Affiliated
            Jinhua Hospital, Zhejiang University School of Medicin, yinfei fang,
            renmin road 365, Jinhua, zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2023
            Assembly Method                   :: SPAdes v. 3.11.1
            Assembly Name                     :: KA04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:21:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,656
            CDSs (total)                      :: 5,535
            Genes (coding)                    :: 5,395
            CDSs (with protein)               :: 5,395
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 60 of 140
            Pseudo Genes (incomplete)         :: 91 of 140
            Pseudo Genes (internal stop)      :: 31 of 140
            Pseudo Genes (multiple problems)  :: 30 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139033.1.
            Bacteria and source DNA available from Affiliated Jinhua Hospital,
            Zhejiang University School of Medicine.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="KA04"
                     /isolation_source="faeces"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pKA04-6"
                     /geo_loc_name="China: zhejiang"
                     /lat_lon="29.06 N 119.39 E"
                     /collection_date="2022-11-27"
     gene            complement(join(7447..8812,1..596))
                     /locus_tag="SH589_RS28215"
                     /old_locus_tag="SH589_28220"
     CDS             complement(join(7447..8812,1..596))
                     /locus_tag="SH589_RS28215"
                     /old_locus_tag="SH589_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_327062021.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1725..1883
                     /locus_tag="SH589_RS28220"
                     /old_locus_tag="SH589_28225"
     CDS             1725..1883
                     /locus_tag="SH589_RS28220"
                     /old_locus_tag="SH589_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_305194776.1"
                     /translation="MGCKQEPPVQPDCCALSGNCSLESNPEKHAKTPLAAAIGNRLAEH
                     KMLKDCH"
                     /name="replication initiation protein"
     gene            complement(2087..2236)
                     /locus_tag="SH589_RS28225"
                     /old_locus_tag="SH589_28230"
     CDS             complement(2087..2236)
                     /locus_tag="SH589_RS28225"
                     /old_locus_tag="SH589_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_305194735.1"
                     /translation="MKKVKTIFLFILIASGFLLVACQANYIRDVQGGTVAPSSSSELAG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2321..2578)
                     /locus_tag="SH589_RS28230"
                     /old_locus_tag="SH589_28235"
     CDS             complement(2321..2578)
                     /locus_tag="SH589_RS28230"
                     /old_locus_tag="SH589_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_305194736.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVKNEWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(2588..4276)
                     /locus_tag="SH589_RS28235"
                     /old_locus_tag="SH589_28240"
     CDS             complement(2588..4276)
                     /locus_tag="SH589_RS28235"
                     /old_locus_tag="SH589_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_327062023.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPNMMSK
                     IMTSLPADTVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     ERNKPRKGTKEHGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKGRKIY
                     EWDSQHGELEGYRASDGKHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4603..4848
                     /locus_tag="SH589_RS28240"
                     /old_locus_tag="SH589_28245"
     CDS             4603..4848
                     /locus_tag="SH589_RS28240"
                     /old_locus_tag="SH589_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_214018610.1"
                     /translation="MKRQRLTEAEERRLAEAIAAEARAMGLRGKDRMPQETRRRIRARV
                     LKSILDERTAGRKREVALTAGQEPEDAFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5154..5417
                     /locus_tag="SH589_RS28245"
                     /old_locus_tag="SH589_28250"
     CDS             5154..5417
                     /locus_tag="SH589_RS28245"
                     /old_locus_tag="SH589_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_094313764.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESGPDKAALLQCLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            5407..5706
                     /locus_tag="SH589_RS28250"
                     /old_locus_tag="SH589_28255"
     CDS             5407..5706
                     /locus_tag="SH589_RS28250"
                     /old_locus_tag="SH589_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094313765.1"
                     /translation="MPYEVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5768..6145)
                     /locus_tag="SH589_RS28255"
                     /old_locus_tag="SH589_28260"
     CDS             complement(5768..6145)
                     /locus_tag="SH589_RS28255"
                     /old_locus_tag="SH589_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_094313766.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTAFDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            6214..6393
                     /locus_tag="SH589_RS28260"
                     /old_locus_tag="SH589_28265"
     CDS             6214..6393
                     /locus_tag="SH589_RS28260"
                     /old_locus_tag="SH589_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094313767.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     EKLWLAMRDHLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6424..6687)
                     /locus_tag="SH589_RS28265"
                     /old_locus_tag="SH589_28270"
     CDS             complement(6424..6687)
                     /locus_tag="SH589_RS28265"
                     /old_locus_tag="SH589_28270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094313768.1"
                     /translation="MTNRVVAVGREALLMVAVMAGTLVVLAGNLLKLLWRVLPVLLLLY
                     GVVQFLARNPEIRDWLFLHFGQGGTAAILVGGILLCMRRLMK"
                     /name="hypothetical protein"
     gene            complement(6716..7447)
                     /gene="mobC"
                     /locus_tag="SH589_RS28270"
                     /old_locus_tag="SH589_28275"
     CDS             complement(6716..7447)
                     /gene="mobC"
                     /locus_tag="SH589_RS28270"
                     /old_locus_tag="SH589_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094313769.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMMMD"
                     /name="mobC"
     BGC             1..8812
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1423..1984
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.18"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(6720..7447)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.004"
                     /coverage="100.0"
                     /evalue="1.97e-140"
ORIGIN
        1 ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct cgtcagcggc cgcaaaatgc
       61 ccgtcaagca gcaggaacgc cagcagccgc gccaggagga cgcctgcgac aagacctccg
      121 gcacaccagg caatcccgaa caggttagcc ggctgcagca ggtaataatc tgcgttcgcg
      181 tagaggtccg gacgcaggac ggtttctccc cagctctcga taaacacggc gacaaccagc
      241 ccccccagaa gggccagacc ggcagcgccg gtcagccagt atgcgtagtt ccagacatgt
      301 cgccgtgccc tgaccatccg cccgggcgaa tccagcgagg ccagcaccga aaatggcgca
      361 gtccggttat tatcgcgctt atagcgatcg gcatactccc gccgcgcctt atgactcagc
      421 cccacgaatc gcatccgccc ggcccagagt tctgccagac cgaaaaacag gccgataacc
      481 agaaaaaaac cggccagact gtaatactcc cggtacagcc agctgaccca cgggtaaatc
      541 tgccgggcca gctccgggat atgccactgc caggcaagcg aatccgtatt aaacatgcaa
      601 tctctctcct tcagacgacg ggtttaccgg cgtgttgtgc agcccctgct gcacaagccg
      661 cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg ttcagctttc
      721 accgcggagg ccagcctctt tccgccccat gcggaaaagg cgcagccccg gaggaacaac
      781 gagcacgccc cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt ttccggtgtt
      841 ccggcccccg tcagagttcg ccatagcgag cggctgacca cccgcaaaat caggagcagc
      901 tttgctgctt cgtcaggtgc gtccagcacc gtggattttg tgggtggtca ggcgtgagcc
      961 tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga gccagaaaga
     1021 gggggagaga ccgtgcgtcg gtggcgtcca gccagcgtaa gcaggggatt tccccctgaa
     1081 gtcagccacg ccccagcggg gatgacctgc atttctccgt aaggagaaaa aagcaacaga
     1141 gataggttaa cgtaaaatat tcagcagaaa accgacaggc ttcacctatc ggaatgattt
     1201 atcggcaggg aagggaaagg ctgtaacact gtgcaggatg tcacatccgc gcgtccgatg
     1261 gccgtctgcg ggagggggga tattgagcct cctcgctcac tcggtcgcta cgctccggcc
     1321 gtgagactgc ggcgggcgct gcggacacat gcaaagttac ccacagattc cgtggataag
     1381 caggggacta acatgtgagg aaaaacggca aaggcagaaa aggccgcgcc ggtggcgttt
     1441 ttccataggc tccgcccccc tgacggactt cacaaaacag acactttttc ggtgcatctg
     1501 tgggaaccgt gaggctcaac catgaaatcc gccagcggtg gcgaaacccg acaggactta
     1561 aagatcccca ccgtttccgg taggtcgctc cctcttgcgc tctcctgttc cgaccctgcc
     1621 gtttaccgga tacctgttcc gcctttctcc cttgcgggaa gtgtggcgct ttctcatagc
     1681 tcacactctg gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag
     1741 aaccccccgt tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc
     1801 cggaaaagca cgcaaaaacg ccactggcag cagccattgg taaccgatta gcagagcaca
     1861 aaatgttaaa ggattgtcac tgagttcttg aagtgatggc ctgactgcgg ctacactaga
     1921 aagacgtatt tggtaattgt gctctgctga agccagttac ccggttaagc agttccccaa
     1981 ctgacttaac cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag
     2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga
     2101 tccccgccag ttcagaggag gacgatggtg ccaccgttcc gccctgaaca tcccggatat
     2161 agtttgcctg gcatgcaacc agaagaaagc cagaagcaat caatataaaa agaaaaatag
     2221 tctttacctt tttcatatac cttccctgta tttaaagact ccagcattca tccctgaaca
     2281 ccggaaatct gtatttcaaa tcatccttga tatattttga ttaccagtta ccatcccggt
     2341 aaacaaagga tacaaagtaa tcaaatttac taatgtcgat tacattttta aagtgtggct
     2401 gtaatatcga aacccactca tttttcacat caaaccaacc attgttaata ttatccttta
     2461 aaggcatgcc aagactttca atgacagaac catcatcacc gaagtctttt gagtattcac
     2521 cgcctttaaa ctcttctgtt ttcttatcaa accaatgaat atgtaattta agtcccatac
     2581 ttacctctta aagatatttt ttaatgtttc gtcctttcgg atccggaccc ttaatttgct
     2641 tacccgtttt tggatcaaat gccccgaggt gtttgccatc actggcacga tacccttcaa
     2701 gctcaccgtg ctgggagtcc cattcataaa tcttacggcc tttatcacca taccatcgtg
     2761 cccgcttccc cccacctcct tgtttagggg ttttcggttt accctctttc aactctccca
     2821 accccttaat atcttcagtc ttggggtcag gaaaataatc atggccgtgc tctttagttc
     2881 ccttacgagg cttgttcctt tcctcattta acttattttc tgcatccttc gcttttttct
     2941 ccttttgttt ccggctctcc atcgccccgg taagtgcagc cttatctctg gataaggatt
     3001 cattcattgc gtttaaagat gcctgagccg cttttaattt ctcattctga ttctgaatat
     3061 ccttatcaag atagcgaacc tgatcctgcc acccggagcc atattcatga ggcgggttat
     3121 aagtcacaaa atcatcttta tttgcctctg cctcttttac ctttttatcg gcttcctgaa
     3181 cagcagccct tgcaccgggg atcgccttct ctgtcgatgc aatgcggctg tttaaggtcg
     3241 caatattttt atcttcggca tccagctcag ctttcgcttt atcatagtct cttttcagcc
     3301 cctcttccgg atgagcggcg tcccatgcct gctggcgacg cttttcttcc tcctgacgct
     3361 gttttacctg tgccggggta agaacatctg tcaccgacac ataaaccggc ttctgtccgg
     3421 tctctttcgg gaaacgaata acggcctcac gggagttacc accggctgta aaaccagccg
     3481 gacgtgaatc acctttttca gcaacaatgc cttttggcgg ggctttcgct gtcggaacac
     3541 ctttcggtac gctcacctgc agagacggga gacccggaat cgacacactg aatacccccg
     3601 gacgttttgt cggtttcgca tccaccacag gaacactcat tggccgaccc gcgacaaccg
     3661 caatatgctg ccgctcatcc ttcaccacat ccacaacccg ttgtctgaca cgaaccgtcg
     3721 cctggtccag tggtaaagta cttgccggag tctccgtcac cgtatcggcc ggtaatgacg
     3781 tcataatttt tgacatcatg ttcggatcat cttttgcaat ctcagaaggc agtacaccgt
     3841 agatcgcaat cccccacaga ccaaacttaa acggaccttt cagggcagcc agcacatcag
     3901 caacggctgc cgacaacgca tcgccggaaa cggataaagc cagtccttca gcccccggag
     3961 tagccagggc aggcagaccg aaggccatcg cagtggccga agactgtccg ccaccagagc
     4021 taccgctatg attgccgctg ttaccaccat taccaccggg tttactcccg ccgttaccaa
     4081 actcccccgg attgtagtta tgaatatctc cgttaggcgt atgcgtcgta ccccagcccg
     4141 gcccgctgtt tggatccgta ccacctcctg atgaagggcc acctttacct gacgtaccgt
     4201 tcacattccc actggcctga ccaccattgt gtcccagacc tgaattaccc ggacctcgtc
     4261 cgtctccgcc actcatatcg ttttcctctt tgacatttaa aacaataaat taaaaccaat
     4321 atactgtata tatatacagt ttaattatta ccttactcct cccgttttca ggtgtcaata
     4381 tatgagatta gttctcatct tcaggcacaa aaaagcggcc ggagccgtct gtcagcaggt
     4441 cgcgtctttt cccgaaaaat atcctgcggc aacaaatttc attatttgag atgtgcatca
     4501 cacttacttt tcattaagta cagaggcatc tgaaagttaa cagatatgag gagagggcct
     4561 gaggttaaac tgttcaggaa accagaaaga ccggaggaca ccatgaaacg tcagagactg
     4621 accgaagcag aagaacgtcg cctagcggaa gccattgccg ccgaggcgcg cgcaatggga
     4681 ctgaggggaa aggacaggat gccacaggag acccgccgcc gcatccgggc gcgggtgctt
     4741 aagtccattc tcgatgagcg gacagccggc agaaaacggg aggtggcact gaccgccggg
     4801 caagagccag aagatgcgtt cacctggcag ccgcagcgcc gccggtaagg ggtatttacc
     4861 ataacgcgtc attacgcgca ccgctcagac ggtctgaggg catttccggc ggctacggga
     4921 taaaacaggc aacaaaccac ccggaaaacc gccggaaacg cgcccggtaa actttatcct
     4981 aatgcatgcc tatgcacgca actgcatggc gtgcagtttg acgtaaaatg ggtgccgtaa
     5041 gccctttttt accagctttc gtcttcatca gcctgactga cacgagcata ttctttcaga
     5101 ccgccccctg ctaaacttct tcatatcacc ccattaatcc gggagtctcc gcaatgagca
     5161 acactacaca ctacgaaaac gccaacttcc tgcgggaact ggctgaaaac ctgccccgca
     5221 tactgccgga aagtggcccg gacaaagctg cgttgttgca atgcctggct aacgaagaac
     5281 tggcccaggc agagtatgaa gatcaggttc gcgccaaagt aaccgccgcc cgtgctgaca
     5341 cgcgtccggg catgaccacg gaacaactcc gccagcgcct gcatggccgt tatcaggaac
     5401 tgcgcgatgc cgtatgaggt ggaatggaaa cagggagcca tcgaggatgt aacgacgctg
     5461 tttgactata tcgcggaaaa ttcatccctg tgggatgcgc agaacgttac cgatcgcgtt
     5521 ctcactgcgg cggacaaact ggccgacttc ccgcgcctct atgaagtgga ctcacgctat
     5581 ggcgaggacg ttcgccggat cagtctgatg ggacagcatg tcctgtatga tgttgatgac
     5641 cagaccagga aagtccgggt tctggccgtg gttggccagc gccagaatcc ccataccgtt
     5701 cgctgataac cggtgacggg ggcgcaattc attgcgtggc cgtcgccggt tggacaagca
     5761 cagcgcgtca gcgttttccc cactgccacc accggcgcgg tgttccggcc ggtgtctctg
     5821 ctgtctccgg tttgtgttcc agcagtctca tggcctgcat ttcctgccgc agtccggcca
     5881 cctcctccct gagtgctttt atctccgcga tcaacgcgtc gaaggccgtt ttttcagtct
     5941 catccggcag tctcaacccg tcccgctcgt gagtctcacc ctgtcttaac tcaccgtaca
     6001 cccggataag ctcgctggta tccatcaccc gaccaccatc agcttccgta cggtatgata
     6061 cgcggccttt cgccatatcc cgatacaggg aactccgggc tttccccgtc agttccgtcg
     6121 cctgtctcac cgtcacccac gccattgtct caccccgcta tttttgagac agtatacccg
     6181 acagacgcag accgtgcccg gaaggacaga aacatgacga agcaggaaaa aaccgccctc
     6241 aacatggcga aattcattaa ggaccagtcg ctgctgttgc tcgagaaact gaacgagctg
     6301 gatatggata ccccggccga catgtgcgaa cggctgcatg aggatgcaga aaagctctgg
     6361 ctggcaatgc gggaccatct cggggaggaa taacagcacc ggccgagccg gtgctgtccg
     6421 gcattatttc atcaggcggc gcatacagag gagtattccc ccgacaagaa tggccgccgt
     6481 gcctccctgc ccgaaatgca gaaacagcca gtcacggatc tccgggttcc tcgccagaaa
     6541 ctgcacaacc ccgtagagca gcagaagcac cggcagtacg cgccaaagca gtttcagcag
     6601 gtttccggcc agaacgacca gcgtcccggc catgacagcg accatcaaca gggcttcacg
     6661 ccccacggca acaacacgat ttgtcataaa aacctctctg agtgaatgta taagctcagt
     6721 ccatcatcat gaatttaaag cacgcaaaca gccgctcccg gtccagctcc accgttttgc
     6781 gggcctcgcc ggtctgcggg ttcacgattt catccaccgc cagaatagcc cgttgcaccc
     6841 tgacctgcgt cgcccggtcc gggcaaatgt accagatttc atcgatgccg ttcgcccggc
     6901 ggttaaacag atacccggac accacctcct gatagcgcct gcggctcttc acggtgcgtt
     6961 ccacctcaaa cgccaccgtt ctgccgtcca gctccgccag cgcgtccggc accttcatcc
     7021 ctttcagggc aatacggtgc aggtaccgcc agccggtggc cccccgggcc tccatggcga
     7081 ggcgcaggga ctgcacggcc agccggtgcg gcagctgcgc cgccgacacc cgcgacggct
     7141 gaaacggaat aatgtccgtc agcggctcgt cctcgtcgaa cgagaacgcc accccgtgcg
     7201 gcgtcagccc ccacacgctg acctgacggc cgtcaaccgg gtatttcgcc cggaccagca
     7261 gccctttcgc caccaggctg tccaggaagc ggtaaaccgg ctgtaccgcc gcaatcccca
     7321 gccgttgcgc aaccagctcc gccgtggaac acagttcttc ccgcagccag cgcagggtca
     7381 gccgtgtttt ctccgcgttc cgtgcctgcc gcttcgcgtg gctgacgttg tatcgctcca
     7441 gtgccatcag tacagccccg caaaatcatc atcacccggc cgctgccgcc cggcctccga
     7501 ctggtactcc gccagcgggt ccgttttcgc cagcacaggg aaggttttca gcggaatttc
     7561 ccggcgatca acacgcagcg ggctgctgaa cgccagccgc gccacgccca gcccggtcat
     7621 caccgcgaac ccgtcaggca gatgttgcag ggtgttcacg tcaaacagcg gccgcgcctc
     7681 cttctggatg aagctgtccc cgctgatatg ctccacgttc cccgcctccc ggctggcccg
     7741 gcggcgctcc acgtccaccc ggatctcccc ggtctgtccg gccgcccact gagcggactc
     7801 ctggctggcc gcccggtaga accagcggat cggcgtgtta tccagtaccg tggttttcac
     7861 aaaatcagcg ggcagatcct gcccgcactg gccgaagtcg cccagcgact ggtgcgcgag
     7921 cagcaggttg cagtcccggc tgcgcagcgt ccccagcgcg ttcagcacgt acttcgacag
     7981 cagatattta atctcatcga gcatgatgct cgcgtgcggc cacctccgga actcgtcccg
     8041 cgcaatgatg atttgcgcgc agcgggcaaa cagcatcttc tgcacccgga tcaccgcctc
     8101 atcgtccatc gagccgatga cgtacaggca gccgccgccg ttgattatcc ccgccacgtc
     8161 gatgccgctg tccgtctgaa ggacgctcag gtcggccacg ttctccagct tggtgatcag
     8221 ccccttcacg tcctttttca gggcctccgg cagcgcgtac gccgccgcca gcacctgctg
     8281 aatatttgcc ccctgcggaa actgctccgc aatcagcttc gccgctttct gctcgctgat
     8341 gcggtagtgg tccgccacgt cgccggtctc actcaggtta aatccggcaa ccagaagatt
     8401 cttcagggcg tactggtcga gatcgcggaa cagattcagc tgcgccggtt tccccggccg
     8461 cagatccagc agggtgaagt ccgggcagtg cgcccggaag acgtgcggcg cccacgcgtc
     8521 ccctttcggg tcgaagtaca ccaccgcgtc cccgaaatcg cgtacgcagc gaatgagcgc
     8581 gttggtggcc atcacgctct tgccgctccc gggcaggccg aggatctgaa tgttggtctt
     8641 gcgccaggta tcacgcggga ccagcaccgg ctggccctgc tcatccttac ccagaaacac
     8701 cgcctgctgt ttccgggcct gctccgtgga aaaatcggcc agcgcgttaa ccggtaccgg
     8761 ctccccgaaa tgcaggtggc ggacgtcggt catttctccg gtccgctggc tg
//
