LOCUS       NZ_CP139032            10079 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella aerogenes strain KA04 plasmid pKA04-5, complete sequence.
ACCESSION   NZ_CP139032
VERSION     NZ_CP139032.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38257105
            Assembly: GCF_034044195.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   fang,y., wu,z. and hu,l.
  TITLE     First Report of OXA-181-Producing Klebsiella aerogenes in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   fang,y., wu,z. and hu,l.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) Department of Clinical Laboratory,Affiliated
            Jinhua Hospital, Zhejiang University School of Medicin, yinfei fang,
            renmin road 365, Jinhua, zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2023
            Assembly Method                   :: SPAdes v. 3.11.1
            Assembly Name                     :: KA04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:21:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,656
            CDSs (total)                      :: 5,535
            Genes (coding)                    :: 5,395
            CDSs (with protein)               :: 5,395
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 60 of 140
            Pseudo Genes (incomplete)         :: 91 of 140
            Pseudo Genes (internal stop)      :: 31 of 140
            Pseudo Genes (multiple problems)  :: 30 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139032.1.
            Bacteria and source DNA available from Affiliated Jinhua Hospital,
            Zhejiang University School of Medicine.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="KA04"
                     /isolation_source="faeces"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pKA04-5"
                     /geo_loc_name="China: zhejiang"
                     /lat_lon="29.06 N 119.39 E"
                     /collection_date="2022-11-27"
     gene            complement(join(9317..10079,1..1199))
                     /locus_tag="SH589_RS28150"
                     /old_locus_tag="SH589_28155"
     CDS             complement(join(9317..10079,1..1199))
                     /locus_tag="SH589_RS28150"
                     /old_locus_tag="SH589_28155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_327062021.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2683..2832)
                     /locus_tag="SH589_RS28155"
                     /old_locus_tag="SH589_28160"
     CDS             complement(2683..2832)
                     /locus_tag="SH589_RS28155"
                     /old_locus_tag="SH589_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2917..3174)
                     /locus_tag="SH589_RS28160"
                     /old_locus_tag="SH589_28165"
     CDS             complement(2917..3174)
                     /locus_tag="SH589_RS28160"
                     /old_locus_tag="SH589_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3184..4869)
                     /locus_tag="SH589_RS28165"
                     /old_locus_tag="SH589_28170"
     CDS             complement(3184..4869)
                     /locus_tag="SH589_RS28165"
                     /old_locus_tag="SH589_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_327062022.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5196..5441
                     /locus_tag="SH589_RS28170"
                     /old_locus_tag="SH589_28175"
     CDS             5196..5441
                     /locus_tag="SH589_RS28170"
                     /old_locus_tag="SH589_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_315964662.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKEVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5716..6087
                     /gene="tolA"
                     /locus_tag="SH589_RS28175"
                     /old_locus_tag="SH589_28180"
     CDS             5716..6087
                     /gene="tolA"
                     /locus_tag="SH589_RS28175"
                     /old_locus_tag="SH589_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6084..6302
                     /locus_tag="SH589_RS28180"
                     /old_locus_tag="SH589_28185"
     CDS             6084..6302
                     /locus_tag="SH589_RS28180"
                     /old_locus_tag="SH589_28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            6644..6922
                     /locus_tag="SH589_RS28185"
                     /old_locus_tag="SH589_28190"
     CDS             6644..6922
                     /locus_tag="SH589_RS28185"
                     /old_locus_tag="SH589_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6910..7224
                     /locus_tag="SH589_RS28190"
                     /old_locus_tag="SH589_28195"
     CDS             6910..7224
                     /locus_tag="SH589_RS28190"
                     /old_locus_tag="SH589_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7388..7816
                     /locus_tag="SH589_RS28195"
                     /old_locus_tag="SH589_28200"
     CDS             7388..7816
                     /locus_tag="SH589_RS28195"
                     /old_locus_tag="SH589_28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7842..8021
                     /locus_tag="SH589_RS28200"
                     /old_locus_tag="SH589_28205"
     CDS             7842..8021
                     /locus_tag="SH589_RS28200"
                     /old_locus_tag="SH589_28205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8049..8579)
                     /locus_tag="SH589_RS28205"
                     /old_locus_tag="SH589_28210"
     CDS             complement(8049..8579)
                     /locus_tag="SH589_RS28205"
                     /old_locus_tag="SH589_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159210149.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8586..9317)
                     /gene="mobC"
                     /locus_tag="SH589_RS28210"
                     /old_locus_tag="SH589_28215"
     CDS             complement(8586..9317)
                     /gene="mobC"
                     /locus_tag="SH589_RS28210"
                     /old_locus_tag="SH589_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_327062020.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQISVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEGRGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9869
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2001..2580
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8590..9317)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.827"
                     /coverage="100.0"
                     /evalue="3.52e-142"
ORIGIN
        1 ttggtgatca gccccttcac gtcctttttc agggcctccg gcagcgcgta cgccgccgcc
       61 agcacctgct gaatatttgc cccctgcgga aactgctccg caatcagctt cgccgctttc
      121 tgctcgctga tgcggtagtg gtccgccacg tcgccggtct cactcaggtt aaatccggca
      181 accagaagat tcttcagggc gtactggtcg agatcgcgga acagattcag ctgcgccggt
      241 ttccccggcc gcagatccag cagggtgaag tccgggcagt gcgcccggaa gacgtgcggc
      301 gcccacgcgt cccctttcgg gtcgaagtac accaccgcgt ccccgaaatc gcgtacgcag
      361 cgaatgagcg cgttggtggc catcacgctc ttgccgctcc cgggcaggcc gaggatctga
      421 atgttggtct tgcgccaggt atcacgcggg accagcaccg gctggccctg ctcatcctta
      481 cccagaaaca ccgcctgctg tttccgggcc tgctccgtgg aaaaatcggc cagcgcgtta
      541 accggtaccg gctccccgaa atgcaggtgg cggacgtcgg tcatttctcc ggtccgctgg
      601 ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg cctcgtcagc ggccgcaaaa
      661 tgcccgtcaa gcagcaggaa cgccagcagc cgcgccagga ggacgcctgc gacaagacct
      721 ccggcacacc aggcaatccc gaacaggtta gccggctgca gcaggtaata atctgcgttc
      781 gcgtagaggt ccggacgcag gacggtttct ccccagctct cgataaacac ggcgacaacc
      841 agccccccca gaagggccag accggcagcg ccggtcagcc agtatgcgta gttccagaca
      901 tgtcgccgtg ccctgaccat ccgcccgggc gaatccagcg aggccagcac cgaaaatggc
      961 gcagtccggt tattatcgcg cttatagcga tcggcatact cccgccgcgc cttatgactc
     1021 agccccacga atcgcatccg cccggcccag agttctgcca gaccgaaaaa caggccgata
     1081 accagaaaaa aaccggccag actgtaatac tcccggtaca gccagctgac ccacgggtaa
     1141 atctgccggg ccagctccgg gatatgccac tgccaggcaa gcgaatccgt attaaacatg
     1201 caatctctct ccttcagacg acgggtttac cggcgtgttg tgcagcccct gctgcacaag
     1261 ccgcggacat gccccaaggc atggcggcgg cggtcacgtc cgtggtgatt gcgttcagct
     1321 ttcaccgcgg aggccagcct ctttccgccc catgcggaaa aggcgcagcc ccggaggaac
     1381 aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc ccagtgcggt cttttccggt
     1441 gttccggccc ccgtcagagt tcgccatagc gagcggctga ccacccgcaa aatcaggagc
     1501 agctttgctg cttcgtcagg tgcgtccagc accgtggatt ttgtgggtgg tcaggcgtga
     1561 gcctgacggg ggccgggtga atttctggcg tggccggcgt ccagccggac agagccagaa
     1621 agagggggag agaccgtgcg tcggtggcgt ccagccagcg taagcagggg atttccccct
     1681 gaagtcagtc acgccccagc ggggatgacg tgcattcctc cgcaaggaga aaaaagcaac
     1741 agagataggt taacgtaaaa tattcagcag aaaaccgaca gacttcacct atctgaataa
     1801 tttatcggca gggaagagaa aggcggtaac acggagcaat atgtcacatc cacgcgtccg
     1861 gtgaccgtct tgcaggtgcc ggggaaattg cgcctcctcg ctcactcggt cgctacgcgc
     1921 cggccgtgag actgcggcgg gcgctgcgaa ctctcgcaaa gttacccaca gattccgtgg
     1981 ataggcaggg aacgagcatg tgaggcaaaa cggcagggcc gcgccggtgg cgtttttcca
     2041 taggctccgc cctcctgcca gagttcacat aaacagacgc tttttcggtg catctgtggg
     2101 agccgtgagg ctcaaccatg aatctgacag tacgggcgaa acccgacagg acttaaagat
     2161 ccccaccgtt tccggtgggt cgctccctct tgcgctctcc tgttccgacc ctgccgttta
     2221 ccggatacct gttccgccgt tctcccttgc gggaagtgtg gcgctttctc atagctcaca
     2281 cactggtatc tcggcccggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc
     2341 cccgttcagc ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggga
     2401 aagcacgcaa aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt
     2461 ttagctaaac acgaggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac
     2521 agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga
     2581 cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta
     2641 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc
     2701 cgtcagttca gaggaggacg atggtgccac cgttccgccc tgaacatccc ggatataatt
     2761 tgcctgacat gcggccagaa gaaagccagc aacaatcaat ataaaaagaa aaatagtctt
     2821 tacctttttc atatactttc cctgtattta aagactccgg cattcatccc tgaacaccgg
     2881 aagtcagtat ttcaaatcat ccttgataat tttttgttac cagttaccgt cccgataatc
     2941 aaaagctata taacaattat ccacatcagg ttttatttga tttttaaaaa atggctgtag
     3001 taaaggaacc cattctttct tcaaatcaaa ctcaccatta ttaatattat cctttaaagg
     3061 cattcccaag ctttcaatga cagaaccatc gtcaccaaag tctttagaat actcttcccc
     3121 tttaaactct tcagttttct tatcaaacca tgtcaaacgt aatttaagcc ccatatttac
     3181 ctctcaaaga tattttttaa tgttccgttt tggatctggc cctttaacct gcttacccgt
     3241 tttgggatca aatgccccga ggtgttcgcc atcactggca cgataccctt caagctcacc
     3301 gtgctgggaa tcccattcat aaattttacg ttttttatcg ccataccatc gggcccgctt
     3361 accaccacca ccttgtttag gtgttttagg atcacctcgc tttaactctc caagtccctt
     3421 tatgtcttct gttttaggta ctggatgata atcatggccg tagtctttag tccccttgcg
     3481 aggcttgttc ttttcctcat ttaacttatt ttctgcatcc ttcgcttttt tctccttttg
     3541 tttccggctc tccatcgccc cggtaagtgc agccttatct ctggataagg attcattcat
     3601 tgcgtttaaa gatgcctgag ccgcttttaa tttctcattc tgattctgaa tatccttatc
     3661 aagatagcga acctgatcct gccacccgga gccatattca tgaggcgggt tataagtcac
     3721 aaaatcatct ttatttgcct ctgcctcttt taccttttta tcggcttcct gaacagcagc
     3781 ccttgcaccg gggatcgcct tctctgtcga tgcaatgcgg ctgtttaagg tcgcaatatt
     3841 tttatcttcg gcatccagct cagctttcgc tttatcatag tctcttttca gcccctcttc
     3901 cggatgagcg gcgtcccatg cctgctggcg acgcttttct tcctcctgac gctgttttac
     3961 ctgtgccggg gtaagaacat ctgtcaccga cacataaacc ggcttctgtc cggtctcttt
     4021 cgggaaacga ataacggcct cacgggagtt accaccggct gtaaaaccag ccggacgtga
     4081 atcacctttt tcagcaacaa tgccttttgg cggggctttc gctgtcggaa cacctttcgg
     4141 tacgctcacc tgcagagacg ggagacccgg aatcgacaca ctgaataccc ccggacgttt
     4201 tgtcggtttc gcatccacca cagggacact cattggccga cctgcgacaa ccgcaatatg
     4261 ctgccgctca tccttcacca catccacaac ccgttgtctg acacgaaccg tcgcctggtc
     4321 cagtggtaaa gtacttgccg gagtctccgt caccgcatcg gccggtaatg acgtcataat
     4381 ttttgacatc attttcggat catcttttgc aatctcagaa ggcagcacac cgtagatcgc
     4441 aatcccccac agaccaaact taaacggccc cttcagggca gccagcacat cagcaacggc
     4501 ggccgacaac gcatcgccgg aaactgataa agccagtcct tcagcccccg gagtagccag
     4561 ggcaggcaga ccgaaggcca tcgcagtggc cgaagactgt ccgccaccag agctaccgct
     4621 atgattgccg ctgttaccac cattaccacc gggtttactc ccgccgttac caaactcccc
     4681 cggattatag ttatgaatat ctccattcgg cgtatgcgtc gtaccccagc ccggcccgct
     4741 gtttggatcc gtaccaccac ctgatgaagg gccaccttta ccagacgtac cgttcacatt
     4801 cccactggcc tgaccaccat tatgtcccag acctgaatta cccggacctc gaccgtctcc
     4861 accactcata tcgttttcct ctttgacatt taaaacaata aattaaagcc aacatactgt
     4921 acatatacac agtataatta ttaccttact cctcccgttt tcaggtgtca atatatgaga
     4981 ttagttctca tcttcaggca caaaaaagcg cccggagcca cctgtaagca gatcgcgtct
     5041 tttcccgaaa atttctcacg gcaacaaatt ttattatttg agatgtacat cacacttaca
     5101 tttcaggtgg tatagacaca tctgaaagct aactggtatg aggggaggac ccgcggttaa
     5161 actgttcagg aaacctgaga cgcccggagg ccaccatgac acacctgaaa ctgactcccg
     5221 ctgaagaaaa gcgcctggcg gacgcctttg tcgctcaggc actggccatg ggcctgaaag
     5281 ggaacagaac gctgccaaag gaggtaaaac agcaggtgcg ggaaagggtg cttcgcgcca
     5341 ttcaggacga acgtgaagcc aggcgggcgc aggaaatgcg tcgggagaaa tacgaggcgg
     5401 cggaaaccac cttcacctgg cagccgcagc gccgccggta aggggtattt accataacgc
     5461 gtcattatca gcaccgcccg agaagccctc agagcgtttc cggcggctac gggataaaac
     5521 aggcaacaaa tcacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca
     5581 tgcctatgca cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg
     5641 tttatgtcaa tgaacgtggc cgtaaagagt ggcaaatata catgtaatat tttttactga
     5701 caggtcatca acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta
     5761 ctcctgacgg cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag
     5821 tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt
     5881 gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc
     5941 actctgaaca gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca
     6001 gccgttaccc acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa
     6061 aacgcccctc tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt
     6121 tcctcactcc ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc
     6181 cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg
     6241 acgatggacg agtggacaac gtgtcgctta ccggctgtca cttttttttc gggaaacgct
     6301 gacggcaatg aggcactggc tatatcccgc agggcacccg gcaatgcgac aggttataaa
     6361 ttttgaattc agtctgaaaa atgacggctt acaggatccg cgaccagcca ccaccaaaaa
     6421 gttcaatgat gatggttgcc gccagtaaca ggtcctttaa cccgcaggct gcaatagtcc
     6481 tggatgatga gtgagcctgt agcagccctg attagtggaa gcgaagctga aaaatgggga
     6541 agcgaagctg aaaaatgggt agcaaaagcc gtatttctgg caaatagcgc cgccacggat
     6601 taaagtagtc atatgtatta cacatgacta caaaggggct tttatgtcat cagcaacatc
     6661 catcaggctc gatgaagaac ttaaggaccg gctgaaaacg ctggccgatg accggcaccg
     6721 ctcagcgcac gcgctaatgc tggaggcaat caccgaatac atcgaccggg aagagaagcg
     6781 cagccagtac ctgcgggacg gacaagccgc atggcagcac tatcaggaaa cgggtctgca
     6841 ccttactgcg gaagaagcag aagcctggat aagtacctgg ggaactgaga acgagcagga
     6901 tgctccgcca tgccacaggt aacgatttcc gcgctggcac agcgagattt acaacgcctc
     6961 caggactttc tcaaaaccaa aaatcggctg gcagccagaa aagccggtga ggtgatcgtc
     7021 cgggctatcc agcaactgaa aacattgcca gacattggcc gcccggtgcc atttctgccg
     7081 ctggaatatc aggaactggt gatcgggttt ggcgacagtg gctatgtgat gctctaccgc
     7141 cacgaccggg aaatggaccg gattgtgatc gtcaccgtca ggcatcagaa agaatccggg
     7201 tatccggggg cagacagcct ctaataaccg atgacggggg cgcaattcat tgcgtggccg
     7261 tcgccggttt gacaagcaca gcgcgtcaga aaaccacatc ctgaacggaa aaacgggtaa
     7321 aattcagcga tacatttacc gtgagaaatt acacaccttt cacacccgat aaaatcaggg
     7381 gtatacagtg gcgaaaacat tgctcaacct ttcacaggca gcacaagccg cgggcattac
     7441 cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa
     7501 aaacaaccct gtcgtggatg tgtcagaact gatccgtgta tacggaaatg tgaatatccc
     7561 tgagaaacaa attcccggca tttcacaccg tgaaaataca caaaaaaatt tcccacagga
     7621 acagcttctg gcaatgcaaa aggaactggc cgatctccgg caggctgtca ccctgatgct
     7681 ggaagataaa acatcccgag aagaagaacg ccgccagcac gatgatgagc gccggaaact
     7741 ccaggccgaa gtggacagat tgacaacaga actgacacag aagaaaaaac gcttctggtc
     7801 cggctggttt agctgagaac ttaccgaagg gaatggagaa catgacgaag caggaaaaaa
     7861 ccgccctcaa tatggctaaa ttcattaagg accagtcgct gctgttgctc gagaaactga
     7921 acgagctgga tatggatacc ccggccgaca tgtgcgagcg gctgcacgag gatgcggaaa
     7981 cactctggca ggtgatgcag gataagctcg gggaagaata acagcaccgg catggccggt
     8041 gctggcgttt attttttgcc gaacggccac ctccggcggg cgggtttatc ctccggcagt
     8101 tctggcaccg gaacgttaaa atagcggctg ttatattccc gcatcagctc cgctttcagc
     8161 ttctcgttca ccgcgctttc cccgtaccgc tcactcacga cgtcaatcat cgccccgatc
     8221 tgctccgggg tgtatttccc ggcgtccagt acctgcacgc cgttctcgat ggcgaactgc
     8281 tcaaccttgc gataaagata atcacacagc ttcattccgg ccggtttctc acgttcccac
     8341 gccggatttt tgccggtgaa gtaatacgtg gctttgtcat cgctgttcag ccgttcgtga
     8401 tacatgccga taaacttcat cattgccaga tgctgggcct gatcttcaaa cctgaataca
     8461 tatgcaccgg tctgccaggc catgaccgcc atgtcttcca gatgctcgct ggttggcata
     8521 atgcagcgct cgccgtcccg gtcctccgcg tcttcaataa ataccatgta cagcgccata
     8581 atccctcact ccgttgtcat aaatttaaag catgcgaaca gcctctcccg gtccagctcc
     8641 gccgttttgc gggcctcgcc ggtctgcggg ttcacgattt catccaccgc cagaatagcc
     8701 cgttgcaccc tgacctgcgt cgcccggtcc gggcaaatgt accagatttc atcgatgccg
     8761 ttcgcccggc ggttaaacag atacccggac accacctcct gatagcgcct gcggctcttc
     8821 acggtgcgtt ccacctcaaa cgccaccgtt ctgccgtcca gctccgccag cgcgtccggc
     8881 accttcatcc ctttcagggc aatacggtgc aggtaccgcc agccggtggc cccccggccc
     8941 tccatggcga ggcgcaggga ctgcacggcc agccggtgcg gcagctgcgc cgccgacacc
     9001 cgcgacggct gaaacggaat aatgtcagtc agcggctcgt cctcgtcgaa cgagaacgcc
     9061 accccgtgcg gcgtcagccc ccacacgctg atctgacggc cgtcaaccgg gtatttcgcc
     9121 cggaccagca gccctttcgc caccaggctg tccaggaagc ggtaaaccgg ctgtaccgcc
     9181 gcaatcccca gccgttgcgc aaccagctcc gccgtggaac acagttcttc ccgcagccag
     9241 cgcagggtca gccgtgtttt ctccgcgttc cgtgcctgcc gcttcgcgtg gctgacgttg
     9301 tatcgctcca gtgccatcag tacagccccg caaaatcatc atcacccggc cgctgccgcc
     9361 cggcctccga ctggtactcc gccagcgggt ccgttttcgc cagcacaggg aaggttttca
     9421 gcggaatttc ccggcgatca acacgcagcg ggctgctgaa cgccagccgc gccacgccca
     9481 gcccggtcat caccgcgaac ccgtcaggca gatgttgcag ggtgttcacg tcaaacagcg
     9541 gccgcgcctc cttctggatg aagctgtccc cgctgatatg ctccacgttc cccgcctccc
     9601 ggctggcccg gcggcgctcc acgtccaccc ggatctcccc ggtctgtccg gccgcccact
     9661 gagcggactc ctggctggcc gcccggtaga accagcggat cggcgtgtta tccagtaccg
     9721 tggttttcac aaaatcagcg ggcagatcct gcccgcactg gccgaagtcg cccagcgact
     9781 ggtgcgcgag cagcaggttg cagtcccggc tgcgcagcgt ccccagcgcg ttcagcacgt
     9841 acttcgacag cagatattta atctcatcga gcatgatgct cgcgtgcggc cacctccgga
     9901 actcgtcccg cgcaatgatg atttgcgcgc agcgggcaaa cagcatcttc tgcacccgga
     9961 tcaccgcctc atcgtccatc gagccgatga cgtacaggca gccgccgccg ttgattatcc
    10021 ccgccacgtc gatgccgctg tccgtctgaa ggacgctcag gtcggccacg ttctccagc
//
