LOCUS       NZ_CP138814             4166 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 11383 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP138814
VERSION     NZ_CP138814.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040185
            Assembly: GCF_033854795.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:43:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,043
            CDSs (total)                      :: 5,921
            Genes (coding)                    :: 5,721
            CDSs (with protein)               :: 5,721
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 80 of 200
            Pseudo Genes (incomplete)         :: 127 of 200
            Pseudo Genes (internal stop)      :: 39 of 200
            Pseudo Genes (multiple problems)  :: 36 of 200
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138814.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="11383"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed8"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-05"
                     /collected_by="He Fang"
     gene            86..199
                     /locus_tag="R8562_RS30185"
                     /old_locus_tag="R8562_30175"
                     /pseudo=""
     CDS             86..199
                     /locus_tag="R8562_RS30185"
                     /old_locus_tag="R8562_30175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            544..1659
                     /locus_tag="R8562_RS30190"
                     /old_locus_tag="R8562_30180"
     CDS             544..1659
                     /locus_tag="R8562_RS30190"
                     /old_locus_tag="R8562_30180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            1659..1862
                     /locus_tag="R8562_RS30195"
                     /old_locus_tag="R8562_30185"
     CDS             1659..1862
                     /locus_tag="R8562_RS30195"
                     /old_locus_tag="R8562_30185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228263081.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKKVFLLY"
                     /name="hypothetical protein"
     gene            1901..2290
                     /locus_tag="R8562_RS30200"
                     /old_locus_tag="R8562_30190"
     CDS             1901..2290
                     /locus_tag="R8562_RS30200"
                     /old_locus_tag="R8562_30190"
                     /inference="COORDINATES: protein motif:HMM:NF022971.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_308221844.1"
                     /translation="MATLRELQISSPSSHNEIAMNGNTKISFFIMPGKDKQGAIEDLII
                     EHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPTNEKKAKVQVYLSCNRESDSRIG
                     ISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            2656..2898
                     /locus_tag="R8562_RS30205"
                     /old_locus_tag="R8562_30195"
     CDS             2656..2898
                     /locus_tag="R8562_RS30205"
                     /old_locus_tag="R8562_30195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(3244..3645)
                     /locus_tag="R8562_RS30210"
                     /old_locus_tag="R8562_30200"
     CDS             complement(3244..3645)
                     /locus_tag="R8562_RS30210"
                     /old_locus_tag="R8562_30200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3645..3836)
                     /locus_tag="R8562_RS30215"
                     /old_locus_tag="R8562_30205"
     CDS             complement(3645..3836)
                     /locus_tag="R8562_RS30215"
                     /old_locus_tag="R8562_30205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..199
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.0"
                     /coverage="92.0"
                     /evalue="3.96e-89"
     Typing          3958..4166
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.429"
                     /coverage="99.0"
                     /evalue="2.44e-75"
ORIGIN
        1 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
       61 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc
      121 ctaatccggt aactatcgtc ttgagtccaa cccggaaaga cacgacaaag cgccactggc
      181 agcagccact ggtaactgat ttagggagtt agtcttgaag ttatacgcca gttaaagcta
      241 aactaaacga acagttttgg tgactgcgct ccctttaagc cagttacctc ggttcataga
      301 gcgccaactc tactgaacct ttcgaaaaaa ccgcccgcca aggcggtttt tttcgttcac
      361 agccggaagc ttacggctgc tgccagataa tctcaagaag aaagtaaaaa aaatcgatta
      421 aaacacaagc aatcaaagtt aatctttgac ataaagtcta gctaatcaca atgaaagctg
      481 tacattttat taaggtacta acaaatcgta tggcatataa caaaaacacg aacagaggct
      541 atcatgataa aggaaataag tttaaagaac ttcaaatgct tcaatgagct atatctaaag
      601 caattaaaga ctttaaatat catagctggg aaaaacaact acgggaaaac atctatactg
      661 gatgctattt tttgttttta tgatgtaaaa aacccggctg ttttgttaaa cattcaagca
      721 ttcagaaaag aaatggcaga aataaacaaa aacaagccat tttgggttag ttactttcat
      781 gatatggaca catcacaaaa gatgtccata gtcattcgag atgaacgctc tgaagtaaca
      841 caaacatatg aaacagaaac aaatcagaga ttagaatcaa gtttgtctct aaacgttgac
      901 accgtactat ctaaccaaaa catacctcgt caaactatcg gttctcaggc cacagtaaac
      961 tctttaaaaa taaatgtgac cgagacaccc aatgcgaaaa acagggtttc caataagctt
     1021 ttctctatgc aattgaaaga agatggcgta gaaataaaat ccgaaataaa aaacgaaagg
     1081 gatgggatta attataaatt taagacagca acaataataa caacatccag aaagataaac
     1141 aaagaggcaa caattacaaa tgtatctagc ctactaacac aaaaaaggaa aaaagacata
     1201 ctagaaaact taaaaaaaat agatgacaga attgtagata tagcaatatc tgcgataggt
     1261 aacaataagg aaatatattt agacattgga tttagtgagc taaatgaaat atcaatgctt
     1321 ggcgagggta tcagtcgagc attatctttt atcagctcag tgctggtaca ggaaaactca
     1381 attattctta ttgatgaaat cgagaacgga atacattact cagtaattaa agacataata
     1441 aaatcattaa tttcatctgc aaaacaaaac aacaatcaaa tatttgcaac aactcatagt
     1501 caagatgtga tacgagcaat aaacgaaata gatagcaaaa atgaagatat cgcttatatt
     1561 agactaggaa gagaaaagaa ctccctaaaa ccaacagccg tacaattcaa tatggatgat
     1621 ttctcctact cagttgagaa cgactgggag gtaagataat gaaagacata aataatagca
     1681 aaaagatcat tttcgttgag ggtgatgatg attttcattt tctatgcaac ctacttgaaa
     1741 tacaaggcat taccgatgtt tttgtcgaaa aaataaatgg aaaatcaaaa ttaaaacaag
     1801 cacttagagc attcaaaaac attgattctt tcgatgaaaa gaaagtattt ttattatatt
     1861 agatgctgac acatcatttt ctgacacaga aagatcaata attgccacat tgcgagaact
     1921 acaaatctcc tcccccagct cgcataacga gatagctatg aatggcaata caaaaatttc
     1981 tttctttata atgccaggaa aagacaagca aggtgccatt gaagatctga taattgaaca
     2041 cgcaagcact cgagcgttat atgaatacat aatagaattt ttcgaaaaaa ttaaaaataa
     2101 taaagatgac atctcagcga gtgacaaaga gtacacttat ccaacaaacg aaaaaaaagc
     2161 taaggtacaa gtctatctta gctgcaatag agaatccgac tcaagaatag gaatatccat
     2221 gaaaaatgga ataataaaca cagcagacaa ttgctttgat gaaataaaag attttatttc
     2281 aaaaatataa atccaaatca agccagccct tcacgggctg gcttatcacc agggtgttat
     2341 taaacgccgg ataacgtata gaaagcatca tacactttag ttatcagcaa tctcaaacgt
     2401 cctaaagaca tcaccagatg ccaaaacaat gattgtgcag atctaatgta aaagacccag
     2461 cacaagatgc tcccttaagt tatattctct ctattcactt aaaacaattt aacataaaat
     2521 cataattgta cgcaccgcta aaacggactc aggacgtttc tgacgggaaa ccggtaaaac
     2581 aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg ctctgttttt acggcatgca
     2641 tgagtatgcg cgcaagtgca tagcgcggaa attcacacca ttcagccagg ggaagtcctg
     2701 tttccggcgt tcttctcctc cgaaaacggt gaacggggac gcaatttatt gcgtggccgt
     2761 gaagcgttgc cggcgcagcc ggaaaaccgg ttttcagcac cttcaggaat cgggttcgtc
     2821 gacggccggc aatcacgtta tggcaagtcg ccggacgact caccacacga ttcaccggcg
     2881 attcgaatcg tgaattgaat caagagtcac catgagcaaa aaacacccaa agtgatgaat
     2941 tgaatcaaga gtcgattcat cataacaata tgataataaa gcgtgattca catatgaatc
     3001 gtgggcaatt cgtcagcgat tcgccataga gtcgtccacg acttggatct gcactttgta
     3061 acacatcacc gcacatttat ttttcattaa cccattgttg taaataagga atctgcaaaa
     3121 ttttaatttt gcgtggggtg tgggtatttt tagtggtgag gagcgttcag agatctgacg
     3181 atttttggga aggttcagga actccctggg atggccggag aggaaaactg aagcatgacg
     3241 gggtcaggat tttttccaga atttccacca tggcgccggg gattcagtta attttgcctg
     3301 gtgattttca agaaggagca tggcctgttt caggctgtca atgtgcgaat ccttggacaa
     3361 aagtaaggtg ttcaggtgtt caatctcctg tttcagatga cggatcacct gttcactctg
     3421 gtcagcagag gctgtatcac gctgtccaga gacctgtcca gaatggtgtt cacccttcgg
     3481 ttgctcaaag gggccaaaga cacgtaaaag ctctgaagta tctaccattt ttgcattgcg
     3541 ctcaccgcta caggtggaca ggtcacctgc tttaattaac ctgtgcagtg ttgttctact
     3601 cttacccgtc agtcgggcag cctcactaat agacactctg gccatcagtt accatcctgt
     3661 aatgcatcca gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc
     3721 tcacaaaggt cggcctcgac atcgagatca agctcgttaa gcttctccag caggagcaga
     3781 gactgattct ggatgaattt agccatgttc agcgctgttt tctgttgttt cgtcataaga
     3841 ggcctcagag cgtcagtggt cagtgtaggg atctcctctt ttccgcttcc tcgctcactc
     3901 agtcgctacg ctcctgccgt tcggctgcgg cgagcggcag gagcatgcgt agaaacgctc
     3961 gagaaaaccg agtaatcgca tggaggaaag aaggaaaggc cgcacagcgg ccgtttttcc
     4021 ataggctccg cccccctgac gagcatcacg gaatctgacg ctcaaatcag tggtggcgaa
     4081 acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc gtgcgctctc
     4141 ctgttcctgc ctttcggttt accggt
//
