LOCUS       NZ_CP138805             4683 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain SEB229 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP138805
VERSION     NZ_CP138805.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040203
            Assembly: GCF_033870115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 16:35:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,615
            CDSs (total)                      :: 5,490
            Genes (coding)                    :: 5,369
            CDSs (with protein)               :: 5,369
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 51 of 121
            Pseudo Genes (incomplete)         :: 73 of 121
            Pseudo Genes (internal stop)      :: 21 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138805.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SEB229"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021"
                     /collected_by="He Fang"
     gene            join(4332..4683,1..347)
                     /locus_tag="R8549_RS28040"
                     /old_locus_tag="R8549_28040"
     CDS             join(4332..4683,1..347)
                     /locus_tag="R8549_RS28040"
                     /old_locus_tag="R8549_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159205176.1"
                     /translation="MTRTFFLLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNALI
                     QNHNLETAALRSHYSGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(354..758)
                     /locus_tag="R8549_RS28045"
                     /old_locus_tag="R8549_28045"
     CDS             complement(354..758)
                     /locus_tag="R8549_RS28045"
                     /old_locus_tag="R8549_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_163535629.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFAPVSVEQAEEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(758..949)
                     /locus_tag="R8549_RS28050"
                     /old_locus_tag="R8549_28050"
     CDS             complement(758..949)
                     /locus_tag="R8549_RS28050"
                     /old_locus_tag="R8549_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1360..1473
                     /locus_tag="R8549_RS28055"
                     /old_locus_tag="R8549_28055"
     CDS             1360..1473
                     /locus_tag="R8549_RS28055"
                     /old_locus_tag="R8549_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            1961..2077
                     /locus_tag="R8549_RS28060"
                     /old_locus_tag="R8549_28060"
     CDS             1961..2077
                     /locus_tag="R8549_RS28060"
                     /old_locus_tag="R8549_28060"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_016947047.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRTFRRRR"
                     /name="small membrane protein"
     gene            complement(2454..2654)
                     /locus_tag="R8549_RS28065"
                     /old_locus_tag="R8549_28065"
     CDS             complement(2454..2654)
                     /locus_tag="R8549_RS28065"
                     /old_locus_tag="R8549_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162092.1"
                     /translation="MKLITVDESVVNAIVEYYAQGSEEAHAEGNRMFLRYLHELTGTQR
                     VAVEAARKQSLAAINKGGASL"
                     /name="hypothetical protein"
     gene            3234..3455
                     /locus_tag="R8549_RS28070"
                     /old_locus_tag="R8549_28070"
     CDS             3234..3455
                     /locus_tag="R8549_RS28070"
                     /old_locus_tag="R8549_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947202.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            3448..4335
                     /locus_tag="R8549_RS28075"
                     /old_locus_tag="R8549_28075"
     CDS             3448..4335
                     /locus_tag="R8549_RS28075"
                     /old_locus_tag="R8549_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_163535632.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKE
                     NRLNHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSLDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLIPSPLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     Typing          998..1473
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.871"
                     /coverage="92.0"
                     /evalue="2.7e-180"
ORIGIN
        1 acagtggtgc tgaggatgta ctgcgggaag cgtttgcgca aaaagaaagc ctcttgcttc
       61 aggcggtgca gaatgcccgg aaagaagcca gaaaggagct ggaagaacag agggaggcac
      121 tgaatcagcg cgaatccatc ctgcagaaaa aggagctggc gctggaaaca accctggaga
      181 aggcccggaa atatgtggcc ggggagcggc ataacaccgt gctggcccgt ctggaagcag
      241 agaaagcaga ggaaagacgg agaaatgcct gcgcgacggc ggaacggcgc agacggaaac
      301 tggaaaaact ccaggccact ggcggaacag cagggcaaaa acactgaggc atttcaggac
      361 tgccagaaac gccaccaggc acggggccgg gtcgtgttct gccgcttctc ttcccggttc
      421 tccagcaaca acatcgcctg tttcaggctg tcgatatgtg aatctctgga ggcaaggagg
      481 gtgttcaggt gttcaatttc ctgtttcaga atgtaaatga cctgttcatt acggtcagaa
      541 gtgactgtgt cacgctgtcc actgacctgt tcttccgcct gttcaacgga taccggcgca
      601 aaggcaccaa acacccgcaa aagctctgaa gtatcgagca ttttcgcatt ccgttcaccc
      661 cgacaggtgg acagttcacc ggctttaatt aacctgtgca gggttgtcct gctcttaccc
      721 gtcaggcgtg cagcctcact gatcgacact ttcgccatca gtcaccctcc tgtagtgcat
      781 ccagtctgag ggacagtgta cggaaaagat gctccgcatc atcgtggagt ttctcacaaa
      841 ggtcagcctc cgcatcgaga tcaagctcgt taagcttctc cagcaggagc agggactgat
      901 tctggatgaa tttagccatg ttcagcgcag ttttctgttg tttcgtcata aggggcctca
      961 gggcgtcagt gctcaatgtc agacggggct cttttccgct tcctcgctca ctcagtcgct
     1021 acgctcctgc cgttcggctg cggcgagcgg acgggacaca cagaagagtc atcgctatca
     1081 ccaggaagag aagagcctgt ggaaaaagcc acggcgttgc cgtttttcca taggctccgc
     1141 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
     1201 cttaaagata ccaggcgttt ccccctggtg gctccctcgt gcgctctcct gttcctgcct
     1261 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt atctcattcc acgcctgaca
     1321 ctcagttccg ggaaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     1381 tccgactgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     1441 aaagcgccac tggcagcagc cactggtaac tgatttagag gagatagtct tgaagtcatg
     1501 cgccagttaa ggctaaacta aacgaactgt tttggtgact gcgctcctct aactagccag
     1561 ttacctcggt tcaaagagtt gctgctctga gaaccttcga gaaaaccgcc ctgcaaggcg
     1621 gttttttcgt ttacagagca agagattacg acgagactga atgaatctca agatttcatt
     1681 tagcctgcca cccgatcatt caaagttttg gcgtagttct gacccgctcc ccgtgatttg
     1741 ttacataccc accacagggc attagttttt tttatttttt tgatgatatg tacaaaatca
     1801 gtggttagga ataatcttag cagccttaac aaacacatac aaaaacagcg gttatgtttc
     1861 atctcaccac aataagtaac gacagtatca tgctaaaaaa agatgatatt atctttatga
     1921 aatcataaca ggctgttttt attaaattaa tcaggcattt atggactgga tttcacttgc
     1981 tatcgccatt gctctacttg ttgtagctgt ctactcagga atctcatact tcagagaaaa
     2041 aaaaacaatc agtcgtacat ttaggcgtag gcgatagcgc cctatttacc ataatccctt
     2101 cttacgcgca ccgcagaaac ggcctcaggg cgtttctggt ggttaacggg caaaaggtgg
     2161 tggcaagtac accgaaaacc gccagaaacg cgcctgattt attttacgca tatgcatggc
     2221 tatgcacacc accgcatagc cggtgatttc ggccgagaaa agggccccgg acgttaaaag
     2281 cataaaagaa cgggccatcc aggcccgtca aatgaggaca actctgtaaa aggaccatcg
     2341 cacctggtgc ggtgccggcg acaagaaaaa gatagctcat atcagcccct caccggagaa
     2401 aaatatttcc gggccagtgc acagacatca gccgtccata aatgcgattt taatcacagg
     2461 gaagcgcccc ccttatttat cgcggccaga gactgctttc tggccgcctc aaccgccaca
     2521 cgctgtgttc ccgttaactc gtgcagataa cgcaggaaca tcctgtttcc ctctgcatgc
     2581 gcctcctcac tgccctgtgc gtagtattcc actatcgcgt tcaccacgga ttcatccacc
     2641 gtaatcagct tcattctgtc tctccgcttt ctctgtgaca ccatcctggc atcacgacgg
     2701 tcatcctggc gtgaaaacgg tgaccgggca ggcaaatttt ttgtctgtcc ggtcaccgtt
     2761 ggcggggtcc ggggcggtca gtcccggtgt tttttattca ggcaatatga gcacctgcga
     2821 cacccgctat gtacgacgca tgaatgcgcc tgtacatagc gggtgtcgct attgagcgga
     2881 gtcaggcgca acatttaaaa aacaacattc acacgcacga aaaacctggc tagtatttag
     2941 tcaaaccatg aggaggcgca ctctgacgcg cacctgccta ccggcagcag gtaaaacagc
     3001 cgggtccctc cggggatact gtaccgggca gcccaatccg agaatcctga gaaaacagct
     3061 aaaatctgcg agaccggcaa tcaggtcagg ccattccctg gcttaaggga cgttatcagc
     3121 cgcgagcagt ccgggctgaa ccacgagagg ttcgaacgag ggcgatcggc gcatgccgca
     3181 gtgaccgtta acgtaacggc cactgatgtt cttacaccca atccggagca cagatgcaat
     3241 ttgataccgc tgccgaactg gccgccctgc aggcgcagac ccgccgtatc cgccaggtcc
     3301 gctaccgtcc ctcccgcctt gaccgctata ccggcgaact gctgagcctg taccaggccg
     3361 gggccagtgc agcggagctg caacgctggc tccgggcccg gcgaatcaag gtggtgttgt
     3421 ccacggttac ccgctggctg gaaaaaaatg gctaaattcg ccagcgctgc ccgccaggca
     3481 gctggcgtga taaaaccact ccagggaaat attctgcgct ctgtcggcac agtccggaat
     3541 tacgagcagg gactgacccg cgtcgcagag tggatgaaag agaaccggct caaccacggc
     3601 gatttacgca cactgacacc ggcgcaggcc atcacctacc tggagcagcg cggggagcag
     3661 gtcggccaga aaacgctcga tatggaacgc caggccatcc aggccatgat gatccacgtg
     3721 acgcagaccc tccctgccgg cgaacacctg ccggtggtga aatcggagca ggcacagatc
     3781 ctgagcagcc gggcctacag cctggaccag gtagccctga ttgccgcagc acaacagccc
     3841 gccaacgccc tggccaccgc gattgccctg gccgccggat tacgcgccca tgaactgctg
     3901 accctcgcgc cccgccagga gcgtgcaccg gatccccgtc cggcagcgga cagtaaattt
     3961 gccggtcggg agggtgtgat ttacaccgtt cagggcaaag gcggcctggt cagggaagtc
     4021 ctgataccgt cccccctggc tgaacgtctc gagcatgtca ggctggcgag cccggtccgg
     4081 gtgaccgatc gcggcgtgtt ttaccagagt cagtacgcca ttggcggagg acagcgctgg
     4141 tcgaacgcct tcagtgccgc ctccatgcgt accctgggct ggtcacgggg ggcacacggc
     4201 gtgcgtcaca gctacgccca gcaacggatg caggagctgc aaaaactcgg tctggttcgt
     4261 gatatggctc tgcgcaccgt cagccaggaa atgggccatt ttcgtccgga aatcacggag
     4321 acatacctgc gatgacaaga acgttttttt tattgtgtac cggtgttttc gccatcgcgt
     4381 acgccctgta tgagctgaat cactatggcg tttactatcc ggacgtcagc aatcctgagg
     4441 cgtttactgg ctggaaggcg gtcaccgaac tcggtcccgc tatcctggca acggggatcg
     4501 cagccacagc gttctcgctg tggctgttca ttttgttttt cagaggctca gagtcccgcc
     4561 ggatacagcg cgagataagc ggatatcagc aggcactgag ggaggcagaa gaggctcaca
     4621 gagccagaca aaatgccctt attcagaatc acaacctgga gacagcggcc ctgcgaagcc
     4681 act
//
