LOCUS       NZ_CP138800             4858 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain T14441 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP138800
VERSION     NZ_CP138800.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040202
            Assembly: GCF_033869935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:36:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,856
            CDSs (total)                      :: 5,733
            Genes (coding)                    :: 5,549
            CDSs (with protein)               :: 5,549
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 69 of 184
            Pseudo Genes (incomplete)         :: 125 of 184
            Pseudo Genes (internal stop)      :: 34 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138800.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="T14441"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021"
                     /collected_by="He Fang"
     gene            complement(165..566)
                     /locus_tag="R8553_RS29250"
                     /old_locus_tag="R8553_29250"
     CDS             complement(165..566)
                     /locus_tag="R8553_RS29250"
                     /old_locus_tag="R8553_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_074075293.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHYGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLSSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(566..757)
                     /locus_tag="R8553_RS29255"
                     /old_locus_tag="R8553_29255"
     CDS             complement(566..757)
                     /locus_tag="R8553_RS29255"
                     /old_locus_tag="R8553_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_116484557.1"
                     /translation="MTKQHKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1619..3337
                     /locus_tag="R8553_RS29260"
                     /old_locus_tag="R8553_29260"
     CDS             1619..3337
                     /locus_tag="R8553_RS29260"
                     /old_locus_tag="R8553_29260"
                     /inference="COORDINATES: protein motif:HMM:NF012862.3"
                     /GO_function="GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="WP_117105082.1"
                     /translation="MHTENTPKETQKNKLATSIQIDTPSNTNQQQPSTRVENIPQQEKQ
                     ITITDGSKDKINIAILIGISDYGSQSTNLPQCENDVSLAKEIITRTKNPRYTLHISNRC
                     SGQDATDKIINFFRDIKEKEEKIDELFFYFTGHGIAHEKNNGEIESRLVFSDYSPSRIN
                     QTTIPSEFIDNQARNLSPNTYVKIIDSCYSGSKIIKGNIEQREKFLNELSKDEKHYTQI
                     GFNSVYVMASSRSDQVSFANSKFSDFSESLFISLSELPGDVKYRHVVNRLADDFTTKEQ
                     RPVFVTQGSLDEGFGVISKDLKDYIYKVLTTEESTEVPSNKGNRSNEEKILKELAESIR
                     QKTSTKLFSKDDFYDFLKLVNKKTLTLLDLLSPFYTVAIDASEKQIVPNEKFIGDWLKS
                     NTHSYFARVRYKKPESTISDSLASNFLNTTLGRSIQNTKPTSKDSASDPQLIIGFTPVG
                     MIDDAHEENSRVREIRLIPKGEMKILDSILIYLIIINSQERIAIFTSIEACPLIDWDKY
                     SSPKCPAWKVHEIKSANQKVETSNELDTIFNNMKSIAIALINSKSLDQEKNIPEA"
                     /name="caspase family protein"
     gene            complement(3789..4235)
                     /locus_tag="R8553_RS29265"
                     /old_locus_tag="R8553_29265"
     CDS             complement(3789..4235)
                     /locus_tag="R8553_RS29265"
                     /old_locus_tag="R8553_29265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(4288..4653)
                     /locus_tag="R8553_RS29270"
                     /old_locus_tag="R8553_29270"
     CDS             complement(4288..4653)
                     /locus_tag="R8553_RS29270"
                     /old_locus_tag="R8553_29270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_186965635.1"
                     /translation="MNRVVSRPATCHNVIAGRRRTGFLNVLKTGFPAAPATLHGHAINC
                     VPVHRFRMGRTPETGLTLAEWREFSRYALVCILMHAVKTESARFWRVFGWFVACFTGFP
                     SETLPTRFSGARNQALW"
                     /name="hypothetical protein"
     Typing          879..1290
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.576"
                     /coverage="99.0"
                     /evalue="6.73e-81"
ORIGIN
        1 caccaactta aactactgat attaaataca aatccgcaaa atgttaattt tgcgtggggt
       61 gtgggtattt ttagtggtga ggagcgttca gagatctggc gatttttggg aaggttcagg
      121 aactccccgg gatggccgga gaggaaaact gaagcatgac ggggtcagga ttttttccag
      181 aatttccacc atggcgcagg ggattcagtt aattttgcct ggtgattttc aagaaggagc
      241 atggcctgtt tcaggctgtc aatgtgcgaa tccttggacg aaagtaaggt gttcaggtgt
      301 tcaatctcct gtttcagatg acgaatcacc tgttcactct ggtcagcaga ggctgtatca
      361 cgctgtccag agacctgtcc ataatggtgt tcacccttcg gttgctcaaa gggaccaaag
      421 acacgtaaaa gctctgaagt atctaccatt tttgcattgc gttcaccgct acaggtggac
      481 aggtcacctg ctttaattaa cctgtgcagt gttgttctac tcttacccgt cagtcgggca
      541 gcctcactaa tagacactct ggccatcagt taccatcctg taatgcatcc agtctcgagg
      601 acagtgtacg gaaaagatgc tccgcatcat cgtggagttt ctcacaaagg tcggcctcga
      661 catcgagatc aagctcgtta agcttctcca gcaggagcag agactgattc tggatgaatt
      721 tagccatgtt cagcgctgtt ttatgttgtt tcgtcataaa tggcctcaga gcgtcagtgc
      781 tcagtgtaaa accagtctct tttccgcttc ctcgctcact cagtcgctac gctccggtcg
      841 ttcggctgcg gcgagcggca ggagcatgcg tataaaagct cgagaagacc gagcggtcaa
      901 atggggaaga aggaaaggcc gcgaagcggc cgtttttcca taggctccgc cctcctgatg
      961 aacatcgcag aaatctgatg atttccggcg ctcaaatcag aaggggcgaa acccgacagg
     1021 acttaaagat accggacgtt tccctccggt ggctccctcg tgcgctctcc tgttcccacc
     1081 ttacagctta gcccgtgtct ttccgctgtt atggccgcgt tttctcatac cacgcctgac
     1141 actgggtgct gcttaggtgg ttcgctccaa gctggactat atgcacgaac tccccgttta
     1201 gtccgactac cacgcctatt caggtaactg ttctcttgat tccaaccctg acagacacga
     1261 caaagcgcca ctggcggtag ccattggtaa aaggtttgcg gagcaagttt gtccaacgga
     1321 ctttacaaga gttcttgaag tgaaagcccg agtacggcta cactgaaagg acagattagg
     1381 tgactgagct ccgctaaagc cagttaccac ggttcaaaag agcgccaact ctcgagaacc
     1441 ttcaaaaaac cgcccgccaa ggcggttttt ttgtttacga gcacagacta cgacgataat
     1501 gcatgaaact caagagttta ggattaagtt ttacttgtgt taaacagtga tataagtgta
     1561 aaaaaacaaa agcaaccttg acgcaatagc tcttccgcta aggataaagg gatagattat
     1621 gcacactgaa aacactccga aagaaacaca aaaaaacaaa ctggcaacca gcatacaaat
     1681 tgatacacca tctaatacaa atcaacaaca accctcaaca agggtagaaa acattcccca
     1741 gcaagaaaaa caaataacaa taactgacgg aagtaaagac aaaataaata tagcaatatt
     1801 aattgggatt tcggattatg gctctcaatc cacaaatcta cctcagtgtg aaaatgacgt
     1861 ttctctagct aaagagataa taactagaac aaaaaaccct agatatactc ttcacatttc
     1921 caatcgctgc tctggacagg atgcaacaga caaaataatt aatttctttc gagacattaa
     1981 agagaaagaa gaaaaaattg atgagttatt cttctacttt actgggcacg gaattgccca
     2041 tgaaaaaaac aatggcgaaa ttgagtctag attggtattt tctgactact ccccaagcag
     2101 aataaaccaa acaacaatac cttcagagtt tattgacaat caagctagga acttatcccc
     2161 aaacacatac gttaaaataa tagactcttg ttatagcggt agcaaaataa taaaaggcaa
     2221 tattgagcaa cgagaaaagt tcttaaacga gctatctaag gatgaaaaac attatacgca
     2281 aattggtttt aacagcgtat atgttatggc ctcatcaagg agcgatcaag tctcattcgc
     2341 caatagtaaa tttagtgact tctccgagtc attatttata tcacttagtg aattaccagg
     2401 tgatgtaaaa tatcgccatg ttgtaaaccg cctggctgac gactttacaa caaaagaaca
     2461 gcggccagtg ttcgtgacgc aaggatctct cgatgagggg tttggtgtaa taagtaaaga
     2521 tttaaaagat tacatctaca aggttttaac aactgaagaa tccactgagg taccaagcaa
     2581 caagggtaac agaagcaatg aagaaaaaat actaaaagaa ctagcagaaa gcataagaca
     2641 aaaaacatct acaaagttgt ttagtaaaga cgacttttat gattttttaa agttagtcaa
     2701 taagaaaaca ctcacactcc ttgatcttct atcgcctttc tacacagtag caattgatgc
     2761 aagtgaaaaa caaatagttc caaatgaaaa attcataggt gattggctaa aaagcaacac
     2821 tcactcttac tttgctaggg ttagatataa gaaaccagaa agcaccataa gtgatagctt
     2881 agcatcaaat ttcctgaata caacactagg aagaagcatt cagaatacaa aaccaacttc
     2941 aaaagacagt gcttcggacc ctcaactaat aattggattc actcctgtag gtatgattga
     3001 tgacgctcat gaagaaaaca gcagggtccg agaaataagg ttaataccta agggtgagat
     3061 gaaaatattg gattcaatat taatttactt aatcataatt aattcacagg aaagaatcgc
     3121 aatttttaca tctatagagg catgcccact aattgattgg gacaagtact catcaccaaa
     3181 atgcccagca tggaaagttc atgaaataaa gtcagcgaac caaaaagtcg aaacatcaaa
     3241 tgaattagac actattttca ataacatgaa gtccatagca atagccttga taaattcaaa
     3301 gagtcttgat caagaaaaaa acattccaga ggcatagtaa taatagttac gtcaggtaaa
     3361 aaaggccctc acatcagtgg cctttttcat atttaatcct agaaggggtc agtttggata
     3421 tcggaaactt ttgtacgtta agcgcattat ttaaccaata ttgatgcgta agggattgat
     3481 tttgcctcag ctacgatcgc ttttaagtca ccgctctttc tgtatcgcat ttaggttatc
     3541 cgattaagga aaattcacgg gaaacggcgt atagcgcggg agtacatcat tgcgagaggt
     3601 aagcctgttc aaaacacagg cttaccgtac aataattctc tatatccaaa ctgaccccaa
     3661 gaagtctgac actaacccca cacaaatcga atccgttgca agatgagaca cacaagaaaa
     3721 gagctagacg aaggtcgctc cgctccccat ggtttttgta tacccaccat gggggcatag
     3781 gtagtttatt aactaactcc aagataatgc gtgatcgcag ccgtgagtac accacagccc
     3841 acttgtttag ataaatctaa aaccccttgt aggattctct tagtgttcca ctcaccttct
     3901 gcatccattg gttgagatag tgcagcactc aactggttct taatcttttg cgaagttata
     3961 tcgttatcta ttgagttgat ttcctccttg gctttctcca aggaagcctg gagttcaccg
     4021 cgttctattt ttaacttttc acgataactg tctggtgtgt gtagttcgtt cccatccgca
     4081 tcgagattaa taacttcgcc tcgctcacca acagtaacaa agctctctac gtgagacaga
     4141 tcatcaacaa ccatcacatt ccttttcatt cttaaaccag tcacttttgc acctgcaccg
     4201 atcttcataa aactaaagcc tgatttatcg ttcactcaca tcacctcata agataaaaat
     4261 gttaataacg ttttcacgag caattattta ccataacgcc tgattacgcg caccactgaa
     4321 acgggttggc agcgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac
     4381 ccgccagaaa cgcgcagact ctgtttttac ggcatgcatg agtatgcaca caagtgcata
     4441 gcgcgaaaat tcacgccatt ccgccagggt aagtcctgtt tccggcgttc ttcccatccg
     4501 aaaacggtga acggggacgc aatttattgc gtgaccgtga agcgttgccg gcgcagccgg
     4561 aaaaccggtt ttcagcacgt tcaggaatcc ggttcgtcga cggccggcaa tcacgttatg
     4621 gcaagtcgcc ggacgactca ccacacgatt caccggcgac tcttcactag agtcgtaccc
     4681 agagtcgcca cgtctaaaaa acaaccaaac tgggagtcgt gtctagagtc gggtcgttat
     4741 taactcattg tttttatagt cctgagtcgt taccgactct atgacgactc gccggggact
     4801 ctatgacgag tcgtcataga gtcggatcta tataaatcaa atttaaaaaa cgaaattt
//
