LOCUS       NZ_CP138799             5290 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain T14441 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP138799
VERSION     NZ_CP138799.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040202
            Assembly: GCF_033869935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:36:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,856
            CDSs (total)                      :: 5,733
            Genes (coding)                    :: 5,549
            CDSs (with protein)               :: 5,549
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 69 of 184
            Pseudo Genes (incomplete)         :: 125 of 184
            Pseudo Genes (internal stop)      :: 34 of 184
            Pseudo Genes (multiple problems)  :: 39 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138799.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="T14441"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021"
                     /collected_by="He Fang"
     gene            complement(97..1086)
                     /locus_tag="R8553_RS29225"
                     /old_locus_tag="R8553_29225"
     CDS             complement(97..1086)
                     /locus_tag="R8553_RS29225"
                     /old_locus_tag="R8553_29225"
                     /inference="COORDINATES: protein motif:HMM:NF013884.3"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="WP_319795799.1"
                     /translation="MFLIVAHHYSVHGGWSYPVGLDIHKFYAQSLSFGGKLGVNLFVLI
                     TGYFSYASRFKWGGVVNTLSKTWFYSISIVLIFYISSPSLITMKDLIKGMLPFGYWFVT
                     TYLVLLVMSPFINAFLSNLSKQKHFKFLVAFTLIAITPLLNSPIGNLSFFVYLYCLGAY
                     IKEYLEDDVVSGKVLLFLVFGGFFLVLSSIASLDFLAGFNEVFNHPLYFIGSSSPFIIM
                     MSIGIFIWFKQMQIGEVRFINIVSSAMFGVYLIHDNSLVRKFLWDSTFQNREHLNSDVY
                     LHSVVAISSVFVVCVVIELLKNKAFDLLNVKSIQVRISRWLDDKLPLI"
                     /name="acyltransferase family protein"
     gene            1271..1968
                     /locus_tag="R8553_RS29230"
                     /old_locus_tag="R8553_29230"
     CDS             join(1271..1520,1520..1968)
                     /locus_tag="R8553_RS29230"
                     /old_locus_tag="R8553_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_319795704.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASSRIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSHQRWLFYAYDRIRRTVVAHVFGERILATLERLLSPLSAFKVVVW
                     MTDGWPLYEPRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(2425..2793)
                     /locus_tag="R8553_RS29235"
                     /old_locus_tag="R8553_29235"
     CDS             complement(2425..2793)
                     /locus_tag="R8553_RS29235"
                     /old_locus_tag="R8553_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057104109.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPENGFFLSGNDFMRAEEE
                     SQRMVESFRDSLGRKKGF"
                     /name="hypothetical protein"
     gene            complement(3177..3326)
                     /locus_tag="R8553_RS29240"
                     /old_locus_tag="R8553_29240"
     CDS             complement(3177..3326)
                     /locus_tag="R8553_RS29240"
                     /old_locus_tag="R8553_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162854964.1"
                     /translation="MPSEYDEQRDATDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REVL"
                     /name="hypothetical protein"
     gene            4316..5011
                     /locus_tag="R8553_RS29245"
                     /old_locus_tag="R8553_29245"
                     /pseudo=""
     CDS             4316..5011
                     /locus_tag="R8553_RS29245"
                     /old_locus_tag="R8553_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     Typing          complement(3320..3534)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.301"
                     /coverage="100.0"
                     /evalue="1.6000000000000006e-59"
     Typing          3484..3998
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.366"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 acaagggtac taaattttat taaaaataaa aaataaaaaa taaaaaatca agaagtcacc
       61 aatggtgacc tcttattcct aatgagttta aaataatcat attaaaggta atttatcatc
      121 caaccaacga ctaattctta cttgaatgct tttaacgtta agcaaatcaa aagccttatt
      181 ctttaataac tcaataacca cacatacaac aaaaacggag gaaatcgcca ctacagaatg
      241 taggtataca tctgaattta aatgctctct attttgaaaa gtagaatccc ataaaaactt
      301 tcttacgaga ctattatcat ggatcaagta aacgccaaac attgccgatg acactatatt
      361 gataaatcta acttcaccaa tttgcatctg tttaaaccaa ataaaaatcc ctatagacat
      421 cataatgatg aacggtgacg aacttccaat gaaataaaga gggtgattaa acacctcatt
      481 aaacccagca agaaaatcta gtgaggctat ggatgacaac actaagaaaa aaccaccaaa
      541 aaccaaaaaa agcagaacct tgccgctaac aacgtcatcc tcaaggtatt ccttaatata
      601 agcacctaga caatagaggt atacaaaaaa acttaagttt cctataggtg agtttaaaag
      661 aggcgtaata gctatcaagg taaacgcaac caagaactta aagtgtttct gtttagataa
      721 attagataaa aaagcattaa tgaaaggaga catcactaac aaaactaaat acgtagttac
      781 gaaccaatag ccaaaaggaa gcataccttt tatcaaatct ttcatcgtta tcaacgatgg
      841 cgatgatata taaaaaatca acactattga gatagagtaa aaccatgttt ttgataatgt
      901 atttacaaca cctccccact taaacctact tgcataagaa aaatatcccg tgattaaaac
      961 aaatagatta acaccgagct ttccaccaaa agataatgat tgcgcataaa atttgtgtat
     1021 atcaagacct acaggataag accaacctcc gtgaactgaa tagtggtggg ctactataag
     1081 aaacattgat agaatcctaa gcaactcaaa gtttgactgt ctgactttca aaatagctcc
     1141 ccaaaaaaca aagattttaa ttcagcaatt ctaccctaag ttcgtcatat gactcttata
     1201 cgaatctcat ttttaggtaa tgctgccaat ttactgattt agtgtgtgat ggtgatttta
     1261 aggtgctcca gtggcttctg tttctatcag ctgcccctcc tgttccgcta ctgacggggt
     1321 ggtgcgtaac ggcaaaagca ctgccggaca tcagcgctat ctctgctctc attgccgtaa
     1381 aacatggcaa ctacagttca cttataccgc ctctcagccc ggtacgcacc agaaaatcat
     1441 tgatatggcc atgaatggcg tcggatgtcg cgccagttca cgcattatgg gcgttggcct
     1501 caacacggtt ttacgtcact taaaaaactc aggccgcagt cggtaacctc gcgcatacaa
     1561 ccgggcagtg atgtgattgt ctgcgctgaa atggacgaac agtggggcta cgtcggtgct
     1621 aaatcacacc agcgctggct gttttacgcg tatgacagga tacggaggac ggttgtggcg
     1681 cacgtcttcg gtgaacgcat tctggccaca ctggagcgtc ttctgagccc gctgtcggcc
     1741 tttaaggtcg tggtatggat gacggatggc tggccgcttt atgaaccccg tctgaaggga
     1801 aagctgcacg ttatcagcaa gcgttacact cagcgcattg agcgacataa cctgaatctg
     1861 agacaacatc tggcaaggct gggacggaag tcactgtcgt tctcaaaatc ggtggagctg
     1921 catgacaagg tcatcgggca ttatctgaac ataaaacact atcagtaagt tggagtcatt
     1981 accctaactt cgaatgatat agtaggtaat atttgttgat ttttttgtga ttttcaatcg
     2041 aaaaaccaca ttcacgacag tatttcggtt gttgtcattt accataacat ccgttgcgtg
     2101 caccgcctga gaggctctga gagcgtttct gacgggaaac cggtaaaaca ggcaacaaac
     2161 cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat gagtatgcgc
     2221 gcaagtgcat agcgcggaaa ttcacgccat tcagccgggg taagtcctgt tttcgacgtt
     2281 cttctcatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg aggcattgac
     2341 caagccgtaa ggcacggtag gtaattgcag gtatccgaca atttcgagta gaggatccac
     2401 tatttcacta cttagaattc aaaatcagaa tcctttcttc ctgccaaggc tatctctgaa
     2461 tgactcaacc attctttgag attcttcttc tgctcgcatg aagtcattcc cggacagaaa
     2521 aaaaccattt tcaggcccac tggcttcaga gaaattcgtt tctgcagcag acacagaatc
     2581 cacatcagca agagacggtt catctgtact ttttctcaat ctgtaaagag ccttatcaaa
     2641 cgcaaccagt gagatattca ggccgtaacg ttcattcagc acagtcacaa tctcttccct
     2701 acggatacca tttttcttcc aggtttctat atcgttcttg gaatccctga cagctgcaat
     2761 ggctgttctt ggaccagggt taaaattcgc catataccgc ccataatgtc ttacatttgt
     2821 ctcataatag tccaaaaata agagtaaaac gcaaaaaatg tcttacaatt gtcttattgt
     2881 tgtcctttcc ggttcatgta tccatgaacc gcctgatgtg gaacgctttt tatcgatctc
     2941 atgcgctgcc aaagatagat tcattcttta aaagtcaggt tacattatcc cttaaatggt
     3001 catacaccat acgcaaccgc gattttcatc gcaattgcat gaattaaaag gtaaaaactg
     3061 aagtccccgt ttgtttatgt aatacgcaac gtattacaac cacctgtaat cacatgaaaa
     3121 taaagaaata agatgtaata ccggcgtatt accacgtacg cgggtaagcg aaaaagctat
     3181 aacacttccc ggccgacagg cggcccaaaa tccaccgggg cgtgcaggtt ctcttcagta
     3241 acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc agtcgcatcc
     3301 cgttgttcat catattcaga tggcatgggg tgatatccag tagctaaggg gttgttagcg
     3361 ggtttcgggg cgcagccctg aaccagtcac gtagcactag cggagtgtat actggctcaa
     3421 tatgttacgg cgagtaacac gtaatgaaag tgcttcatgt gacaggggaa aatgcagcga
     3481 cagggcgtac gggagaaaat gggggttacg gtgatatgac gcctcctcgc tcactcggtc
     3541 gctacgctcc ggccgttcgg ctgcggcggg cgttacctgc tcacgaatga cgaggtaacg
     3601 agatgaagct gaggtcaaga aaggccggaa aaaggatgcg gcattgccgt ttttccatag
     3661 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
     3721 gacaggacta taaagatccc aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt
     3781 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
     3841 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
     3901 ccgttcagtc cgaccgctgc gccttatccg gtaactatca tcttgagtcc aacccggaga
     3961 ggcacgacaa aacgccactg gcagcagcca ctggtaacgg attcgcagaa caaagaagtg
     4021 ttgaagtgtg aggtgaagta cagctacact gtaataacat atttggtttc tgtgctctgc
     4081 gaaagccagt tacctcggtt caaagagttg gtagctcagc gaaccttgag aaaaccaccg
     4141 ttggtagcgg tggttttttc tttatgcgat gatgaatcaa tcagtctatc aagtcaacga
     4201 accgcgcttc cattgacaaa aaaacaagat ctgtacatat tcagaaaaaa caacggattg
     4261 ggtaatggtg ccaacttact gatttagtgt gcgatggtgt ttttgaggtg ctcccgtggc
     4321 ttccatctcc atcagttgtc cctcctgctc agctactgaa ggcgtggtgc gtaacggtaa
     4381 aagtactgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcagctaca
     4441 gttcacttac accgcctctc agcccggtac acaccagaaa atcattgata tggccatgaa
     4501 tggcgtcgga tgtcgcgcca gtgcacgcat tatgggcgtt ggcctcaaca cggttttacg
     4561 acacttaaaa aactcaggcc gcagtcggta aactcacgca tacaaccggg cagtgacgtc
     4621 attgtttgcg cggaaatgga cgaacagtgg ggttacgtcg gcgctaaatc acgccagcgt
     4681 tagttgtttt acgcgtatga caggatacgg aggaggttgt ggcgcacgtc ttcggtgaac
     4741 gcactctggc cacactggag cgtcttctga gcctgctgtc ggcctttgag gtcgtggtat
     4801 ggatgacgga tggctggccg ctgtataaat cacgcctaaa gggagaactg cacgttatca
     4861 gcaagcgata tacgcagcgc attgagcggc ataacctgaa tctgaggcaa gatctggcaa
     4921 ggctgggacg gaagtcactg tcgctctcaa atcggtggag ctgcatgaca aagtcatcgg
     4981 gcattatctg aacataaaac actatcagta agttggagtc attaccctta caaggggtca
     5041 gtttggagat cggaaatttt tgtacgttaa gcgcattatt taaccaatct tggttcgtaa
     5101 ggggttgata ttgcgtcagt ggctatcgct tttaagttgt cgctcttgca gtatcgcatt
     5161 taggttatcc gattaaggtc aaacctctga aaatgccgta tagcgcggga gtacacccta
     5221 ttgctatagg taagtctgtt caaaaaacag gcttaccgta caataattct ctatatccaa
     5281 actgccccct
//
