LOCUS       NZ_CP138791             4236 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain T24441 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP138791
VERSION     NZ_CP138791.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040201
            Assembly: GCF_033869415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:44:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,873
            CDSs (total)                      :: 5,750
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 70 of 186
            Pseudo Genes (incomplete)         :: 126 of 186
            Pseudo Genes (internal stop)      :: 34 of 186
            Pseudo Genes (multiple problems)  :: 39 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138791.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="T24441"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed8"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021"
                     /collected_by="He Fang"
     gene            513..836
                     /locus_tag="R8540_RS29315"
                     /old_locus_tag="R8540_29310"
     CDS             513..836
                     /locus_tag="R8540_RS29315"
                     /old_locus_tag="R8540_29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176142619.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNGGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAE
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1045..>1479
                     /locus_tag="R8540_RS29320"
                     /old_locus_tag="R8540_29315"
                     /pseudo=""
     CDS             1045..>1479
                     /locus_tag="R8540_RS29320"
                     /old_locus_tag="R8540_29315"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1514..1999
                     /locus_tag="R8540_RS29325"
                     /old_locus_tag="R8540_29320"
     CDS             1514..1999
                     /locus_tag="R8540_RS29325"
                     /old_locus_tag="R8540_29320"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_279281588.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTIIREQKSTQAILSERNSGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2003..2218
                     /locus_tag="R8540_RS29330"
                     /old_locus_tag="R8540_29325"
     CDS             2003..2218
                     /locus_tag="R8540_RS29330"
                     /old_locus_tag="R8540_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_063617430.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSNG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2585..3082)
                     /locus_tag="R8540_RS29335"
                     /old_locus_tag="R8540_29330"
     CDS             complement(2585..3082)
                     /locus_tag="R8540_RS29335"
                     /old_locus_tag="R8540_29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_219830950.1"
                     /translation="MKKQILALALLSLPLIGMAKDSGYGNTEWGMTPNQVVAAQKGKAH
                     LIPPQKYKDQWGKVSIEDVNIGSGIYTVTFIFDASDKLVQTNVASKEKKNEGIANDQFN
                     ALNQLLTQKYGKPQFVSDEKATWKTDATTIELNKMIISGIMAQTLVRYIPNKTVISDTS
                     NL"
                     /name="hypothetical protein"
     gene            complement(3092..3679)
                     /locus_tag="R8540_RS29340"
                     /old_locus_tag="R8540_29335"
     CDS             complement(3092..3679)
                     /locus_tag="R8540_RS29340"
                     /old_locus_tag="R8540_29335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019842775.1"
                     /translation="MIRKIFFASAIVVAISGCTAMDKLAGMGVVSQQTSTFDNSTVIEV
                     SPNSLYDPNSTWGTPLQLGAIWSSAYPEEAGLILSYNSNVSSGNAAYLGLTGIDINVDG
                     KISSYKAQQQTNLSSSSYNSVSKTIYTSSKNTIVIPYKLLESMVSAKNCRIRIHTSQGN
                     QDIDFSAQSIPGGKATAIVSIKEFLAKVERFK"
                     /name="hypothetical protein"
     gene            complement(3994..4107)
                     /locus_tag="R8540_RS29345"
                     /old_locus_tag="R8540_29340"
     CDS             complement(3994..4107)
                     /locus_tag="R8540_RS29345"
                     /old_locus_tag="R8540_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059446996.1"
                     /translation="MHEPPVQSDRCALSGNYHLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          226..404
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.912"
                     /coverage="83.0"
                     /evalue="7.48e-53"
     Typing          complement(2..250)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.098"
                     /coverage="94.0"
                     /evalue="5.31e-63"
     Typing          complement(3791..4236)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.755"
                     /coverage="89.0"
                     /evalue="3.01e-115"
     Typing          827..2322
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.184"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taccaggggg aaactcttgg gatctttata gtcctgtcgg gtttcgccgc cactgatttg
       61 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggcagcgccg
      121 tatccttttt ccggcctttc ctgacttgag tgccatctct ttatcttgtc attcgtgagc
      181 aggtaacgcc cgccgcagtc tcacggcagg agcgcagcga ccgagtgagc gaggaggcgt
      241 catgtcaccg ttacctgcat tttgcactta cgccctatag ccgcattttc ccttgcaaca
      301 tgaataactt ccctgaattg ccactctgat ctaacatatt tagccagtat acactccgct
      361 agcgctccgt gactggttca gggctgcgcc ccgaaacccg ctaaaagcca ctgacgcgcc
      421 tgcggcttgt ccaacaccgc gccgtccggg aaagaatttc ccgcccgtcc cggcgttatc
      481 aggaggccgg attggcagat aaacgcagca aaatgctcac catgtgggta acggaagacg
      541 agcaccggcg tctgctggag cgttgcgacg gtaagcagct cgcagcctgg atgcgccaga
      601 cgtgcctgga cgagaagccc gccagagccg gcaaacttcc ctcgctctcg ccggcgctgc
      661 ttcgtcagct tgccggcatg gggaacaacc ttaaccagat tgcccggcag gttaacggcg
      721 gtggcggtag cgggcacgac cgcgtgcagg ttgtcgccgc gctgatggcc atcgatgccg
      781 gactcgagcg gctgcggcac gccgtgctgg aaaagggggc agaggatgat cgttaaattt
      841 caccccagag ggcgtggcgg cggcgccggt ccggtggatt acctgctggg gaaagaccgg
      901 cagcgcgacg gcgccagcgt tctgcagggt aagccggagg aagtccggga gcttatcgac
      961 gcctcgccct acgccaaaaa gtatacatcc ggcgtcctgt cctttgccga aaaggattta
     1021 ccgcccgggc agcgagaaaa gctgatggcg agctttgagc gggttctgat gcccggactc
     1081 gataaagacc agtacagcgt gctgtgggtc gagcaccagg acaaggggcg gctggagctg
     1141 aacttcctga tcccgaatac ggagctgctg accggcaagc ggctccagcc gtattacgac
     1201 cgcgccgacc gtccgcgcat tgatgcctgg cagaccatag tgaacggcag gctggggctg
     1261 cacgacccga acgcgccgga aaaccggcga gcgctggtca cgccttccgc gctgcctgaa
     1321 gcgaagcagg aagccgccca ggcgataacg cgcggcttgc ttgtccttgc ctcatccggg
     1381 gagctgaaaa cgcggcagga cgttacagag gcgctggaaa acgcaggttt cgaggtggtg
     1441 cgcaccacga aaagcagcat cagcattgcc gacccggacg gggggcgaaa catccgactg
     1501 aagggagcca tctatgaaca gtcttttaac gctggcgaag gacttagagc agaaatcgaa
     1561 agcgcagcag cagactaccg gcgagatgct gaaagccgca ttcagcgagc acgagaagtc
     1621 tgtcagagcg gaactgagcg aaagcgagaa gagaatcagc gccgccatcc tcgaccacga
     1681 ccggaagctg tcctcagcca tgagccagcg cacgaaaggg atgctgcgca tggtcagcca
     1741 gacgtggctg accatcgtcc tggtctccgc gctgctgatc gcctcgagcg cgggcattct
     1801 gtggtggcag gggcagcaga tactcgacaa ctacacgatc atccgggagc aaaagagcac
     1861 gcaggccata ctgtcagaga ggaacagcgg cgttcagctc acgacctgcg gggaagagag
     1921 acgccgctgc gtgagggtga acccggaagc gggaagattc ggagaggatt cgagctggat
     1981 gatactggcg gggaaatagc acatgacgga gctggaaaaa cagttgctga gcgcattaga
     2041 gcagctacag caggactact cgaaaaggct ggacgagtgg gagaacgcct tcgcggaatg
     2101 gcggacgatg tctgggctta tgcaacggga gaacgcggcg ctgagcgagc gcgtcacggg
     2161 cttgagcagg caggtgcaga gtttgagcga gcagctgcgc cggttgtcga acggctgaat
     2221 gccgtcgagc tgcatcagca gcaggagcgc gcggcgcagc atcagaaagc gctggagctg
     2281 gagcgatcgc agcggcagcg gacgtataac ggcccgtcgc tgtgataacc gaataaaacg
     2341 gggctttcgg cgggtatctg cgctgaaaac ctgagccatt cacccggctg catggctatg
     2401 cagtccgggg aaatttaccg gacgcaaacg gggcggttta ccgggcgctt tgctgcgggt
     2461 tttaccaagc aggcaccgta ttcacgccga acctgcccgg agcggtgccg ataagagatg
     2521 ttatgttcaa tctcccacct cagatataga aacaggagag agacactcag tgaagctaca
     2581 attattacag gttagacgtg tcagaaatca cggtcttatt aggaatgtag cgaactagtg
     2641 tttgagccat aattccggag atgatcatct tatttagctc aattgttgta gcatcagttt
     2701 tccatgttgc tttttcatca cttacaaact gtggtttgcc atacttctgt gtaagtaatt
     2761 gatttaatgc gttaaattgg tcgttcgcta ttccctcatt tttcttttct ttgctggcga
     2821 cgttggtttg gactaattta tctgatgcgt caaaaataaa cgtaactgta tatattccac
     2881 tacctatatt cacgtcttca atgctcactt ttccccattg gtctttatac ttttgagggg
     2941 ggattaaatg agcttttcct ttctgggctg caacgacctg atttggggtc ataccccatt
     3001 cggtattgcc ataaccagag tcctttgcca tgccaatcag tggcagtgat agcaaagcga
     3061 gtgcaagaat ctgcttcttc atgacgcctc cttatttaaa acgttcgact ttggccagga
     3121 actctttaat ggaaacgatc gctgtcgctt tacctccggg gatactttgt gctgagaagt
     3181 cgatgtcttg atttccttgg cttgtgtgta tgcgaatgcg gcagttttta gctgatacca
     3241 tgctctccag caatttatac gggataacga tagtattctt gctagaggta tagatcgtct
     3301 tggatacgga gttatatgag ctgctgctta gattagtttg ttgctgtgct ttatagctgg
     3361 atattttacc atcaacattt atatcaatcc ccgtcaaacc taagtatgcg gcatttccgc
     3421 tagatacatt cgagttgtag gaaaggatta gtccagcctc ttctggatag gcactagacc
     3481 atatcgcgcc caactgaagt ggtgttcccc aagtgctgtt agggtcgtat agcgaatttg
     3541 gagatacctc tatgacagtc gagttatcaa aggttgatgt ttgctgagaa acaacaccca
     3601 ttcccgccaa tttatccatt gctgtacagc cagagatcgc tactacgatc gcagaagcaa
     3661 aaaaaatctt ccttatcata tcgttatcct atttttttct ttttaacaga gactgaaaaa
     3721 aacatcgtat tcccgaaacc attttgcaca cgatattctt ttttaaaagg attttgaaag
     3781 gatgcgggca gatcttcttg agatccttaa gcgagagtcg taatctcttg accacgaaac
     3841 gaaaaaaccg ccttgcaggg cggtttttcg aaggttctca gagctaccaa ctctttgaac
     3901 cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa
     3961 ccggcgcata acttcaagac taactcctct aaatcagtta ccagtggctg ctgccagtgg
     4021 cgctttgtcg tgtctttccg ggttggactc aagatgatag ttaccggaca aggcgcagcg
     4081 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     4141 actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg acaccggtaa
     4201 accgaaaggc aggaacagga gagcgcacga gggagc
//
