LOCUS       NZ_CP138789             5290 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain T24441 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP138789
VERSION     NZ_CP138789.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040201
            Assembly: GCF_033869415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:44:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,873
            CDSs (total)                      :: 5,750
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 70 of 186
            Pseudo Genes (incomplete)         :: 126 of 186
            Pseudo Genes (internal stop)      :: 34 of 186
            Pseudo Genes (multiple problems)  :: 39 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138789.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="T24441"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021"
                     /collected_by="He Fang"
     gene            complement(442..810)
                     /locus_tag="R8540_RS29265"
                     /old_locus_tag="R8540_29260"
     CDS             complement(442..810)
                     /locus_tag="R8540_RS29265"
                     /old_locus_tag="R8540_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057104109.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPENGFFLSGNDFMRAEEE
                     SQRMVESFRDSLGRKKGF"
                     /name="hypothetical protein"
     gene            complement(1194..1343)
                     /locus_tag="R8540_RS29270"
                     /old_locus_tag="R8540_29265"
     CDS             complement(1194..1343)
                     /locus_tag="R8540_RS29270"
                     /old_locus_tag="R8540_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162854964.1"
                     /translation="MPSEYDEQRDATDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REVL"
                     /name="hypothetical protein"
     gene            2333..3028
                     /locus_tag="R8540_RS29275"
                     /old_locus_tag="R8540_29270"
                     /pseudo=""
     CDS             2333..3028
                     /locus_tag="R8540_RS29275"
                     /old_locus_tag="R8540_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(3404..4438)
                     /locus_tag="R8540_RS29280"
                     /old_locus_tag="R8540_29275"
     CDS             complement(3404..4438)
                     /locus_tag="R8540_RS29280"
                     /old_locus_tag="R8540_29275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.3"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_319795703.1"
                     /translation="MKVRQSNFELLRILSMFLIVAHHYSVHGGWSYPVGLDIHKFYAQS
                     LSFGGKLGVNLFVLITGYFSYASRFKWGGVVNTLSKTWFYSISIVLIFYISSPSLITMK
                     DLIKGMLPFGYWFVTTYLVLLVMSPFINAFLSNLSKQKHFKFLVAFTLIAITPLLNSPI
                     GNLSFFVYLYCLGAYIKEYLEDDVVSGKVLLFLVFGGFFLVLSSIASLDFLAGFNEVFN
                     HPLYFIGSSSPFIIMMSIGIFIWFKQMQIGEVRFINIVSSAMFGVYLIHDNSLVRKFLW
                     DSTFQNREHLNSDVYLHSVVAISSVFVVCVVIELLKNKAFDLLNVKSIQVRISRWLDDK
                     LPLI"
                     /name="acyltransferase"
     gene            4578..5275
                     /locus_tag="R8540_RS29285"
                     /old_locus_tag="R8540_29280"
     CDS             join(4578..4827,4827..5275)
                     /locus_tag="R8540_RS29285"
                     /old_locus_tag="R8540_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_319795704.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASSRIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSHQRWLFYAYDRIRRTVVAHVFGERILATLERLLSPLSAFKVVVW
                     MTDGWPLYEPRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          complement(1337..1551)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.301"
                     /coverage="100.0"
                     /evalue="1.6000000000000006e-59"
     Typing          1501..2015
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.366"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ctaacttcga atgatatagt aggtaatatt tgttgatttt tttgtgattt tcaatcgaaa
       61 aaccacattc acgacagtat ttcggttgtt gtcatttacc ataacatccg ttgcgtgcac
      121 cgcctgagag gctctgagag cgtttctgac gggaaaccgg taaaacaggc aacaaaccac
      181 ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcgcgca
      241 agtgcatagc gcggaaattc acgccattca gccggggtaa gtcctgtttt cgacgttctt
      301 ctcatccgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgagg cattgaccaa
      361 gccgtaaggc acggtaggta attgcaggta tccgacaatt tcgagtagag gatccactat
      421 ttcactactt agaattcaaa atcagaatcc tttcttcctg ccaaggctat ctctgaatga
      481 ctcaaccatt ctttgagatt cttcttctgc tcgcatgaag tcattcccgg acagaaaaaa
      541 accattttca ggcccactgg cttcagagaa attcgtttct gcagcagaca cagaatccac
      601 atcagcaaga gacggttcat ctgtactttt tctcaatctg taaagagcct tatcaaacgc
      661 aaccagtgag atattcaggc cgtaacgttc attcagcaca gtcacaatct cttccctacg
      721 gataccattt ttcttccagg tttctatatc gttcttggaa tccctgacag ctgcaatggc
      781 tgttcttgga ccagggttaa aattcgccat ataccgccca taatgtctta catttgtctc
      841 ataatagtcc aaaaataaga gtaaaacgca aaaaatgtct tacaattgtc ttattgttgt
      901 cctttccggt tcatgtatcc atgaaccgcc tgatgtggaa cgctttttat cgatctcatg
      961 cgctgccaaa gatagattca ttctttaaaa gtcaggttac attatccctt aaatggtcat
     1021 acaccatacg caaccgcgat tttcatcgca attgcatgaa ttaaaaggta aaaactgaag
     1081 tccccgtttg tttatgtaat acgcaacgta ttacaaccac ctgtaatcac atgaaaataa
     1141 agaaataaga tgtaataccg gcgtattacc acgtacgcgg gtaagcgaaa aagctataac
     1201 acttcccggc cgacaggcgg cccaaaatcc accggggcgt gcaggttctc ttcagtaacc
     1261 tgcgccatta aatcaaaaag gtcatattcg ccgggaagct gcgtatcagt cgcatcccgt
     1321 tgttcatcat attcagatgg catggggtga tatccagtag ctaaggggtt gttagcgggt
     1381 ttcggggcgc agccctgaac cagtcacgta gcactagcgg agtgtatact ggctcaatat
     1441 gttacggcga gtaacacgta atgaaagtgc ttcatgtgac aggggaaaat gcagcgacag
     1501 ggcgtacggg agaaaatggg ggttacggtg atatgacgcc tcctcgctca ctcggtcgct
     1561 acgctccggc cgttcggctg cggcgggcgt tacctgctca cgaatgacga ggtaacgaga
     1621 tgaagctgag gtcaagaaag gccggaaaaa ggatgcggca ttgccgtttt tccataggct
     1681 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
     1741 aggactataa agatcccagg cgtttccccc tggtggctcc ctcgtgcgct ctcctgttcc
     1801 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc
     1861 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
     1921 ttcagtccga ccgctgcgcc ttatccggta actatcatct tgagtccaac ccggagaggc
     1981 acgacaaaac gccactggca gcagccactg gtaacggatt cgcagaacaa agaagtgttg
     2041 aagtgtgagg tgaagtacag ctacactgta ataacatatt tggtttctgt gctctgcgaa
     2101 agccagttac ctcggttcaa agagttggta gctcagcgaa ccttgagaaa accaccgttg
     2161 gtagcggtgg ttttttcttt atgcgatgat gaatcaatca gtctatcaag tcaacgaacc
     2221 gcgcttccat tgacaaaaaa acaagatctg tacatattca gaaaaaacaa cggattgggt
     2281 aatggtgcca acttactgat ttagtgtgcg atggtgtttt tgaggtgctc ccgtggcttc
     2341 catctccatc agttgtccct cctgctcagc tactgaaggc gtggtgcgta acggtaaaag
     2401 tactgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc agctacagtt
     2461 cacttacacc gcctctcagc ccggtacaca ccagaaaatc attgatatgg ccatgaatgg
     2521 cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg ttttacgaca
     2581 cttaaaaaac tcaggccgca gtcggtaaac tcacgcatac aaccgggcag tgacgtcatt
     2641 gtttgcgcgg aaatggacga acagtggggt tacgtcggcg ctaaatcacg ccagcgttag
     2701 ttgttttacg cgtatgacag gatacggagg aggttgtggc gcacgtcttc ggtgaacgca
     2761 ctctggccac actggagcgt cttctgagcc tgctgtcggc ctttgaggtc gtggtatgga
     2821 tgacggatgg ctggccgctg tataaatcac gcctaaaggg agaactgcac gttatcagca
     2881 agcgatatac gcagcgcatt gagcggcata acctgaatct gaggcaagat ctggcaaggc
     2941 tgggacggaa gtcactgtcg ctctcaaatc ggtggagctg catgacaaag tcatcgggca
     3001 ttatctgaac ataaaacact atcagtaagt tggagtcatt acccttacaa ggggtcagtt
     3061 tggagatcgg aaatttttgt acgttaagcg cattatttaa ccaatcttgg ttcgtaaggg
     3121 gttgatattg cgtcagtggc tatcgctttt aagttgtcgc tcttgcagta tcgcatttag
     3181 gttatccgat taaggtcaaa cctctgaaaa tgccgtatag cgcgggagta caccctattg
     3241 ctataggtaa gtctgttcaa aaaacaggct taccgtacaa taattctcta tatccaaact
     3301 gccccctaca agggtactaa attttattaa aaataaaaaa taaaaaataa aaaatcaaga
     3361 agtcaccaat ggtgacctct tattcctaat gagtttaaaa taatcatatt aaaggtaatt
     3421 tatcatccaa ccaacgacta attcttactt gaatgctttt aacgttaagc aaatcaaaag
     3481 ccttattctt taataactca ataaccacac atacaacaaa aacggaggaa atcgccacta
     3541 cagaatgtag gtatacatct gaatttaaat gctctctatt ttgaaaagta gaatcccata
     3601 aaaactttct tacgagacta ttatcatgga tcaagtaaac gccaaacatt gccgatgaca
     3661 ctatattgat aaatctaact tcaccaattt gcatctgttt aaaccaaata aaaatcccta
     3721 tagacatcat aatgatgaac ggtgacgaac ttccaatgaa ataaagaggg tgattaaaca
     3781 cctcattaaa cccagcaaga aaatctagtg aggctatgga tgacaacact aagaaaaaac
     3841 caccaaaaac caaaaaaagc agaaccttgc cgctaacaac gtcatcctca aggtattcct
     3901 taatataagc acctagacaa tagaggtata caaaaaaact taagtttcct ataggtgagt
     3961 ttaaaagagg cgtaatagct atcaaggtaa acgcaaccaa gaacttaaag tgtttctgtt
     4021 tagataaatt agataaaaaa gcattaatga aaggagacat cactaacaaa actaaatacg
     4081 tagttacgaa ccaatagcca aaaggaagca taccttttat caaatctttc atcgttatca
     4141 acgatggcga tgatatataa aaaatcaaca ctattgagat agagtaaaac catgtttttg
     4201 ataatgtatt tacaacacct ccccacttaa acctacttgc ataagaaaaa tatcccgtga
     4261 ttaaaacaaa tagattaaca ccgagctttc caccaaaaga taatgattgc gcataaaatt
     4321 tgtgtatatc aagacctaca ggataagacc aacctccgtg aactgaatag tggtgggcta
     4381 ctataagaaa cattgataga atcctaagca actcaaagtt tgactgtctg actttcaaaa
     4441 tagctcccca aaaaacaaag attttaattc agcaattcta ccctaagttc gtcatatgac
     4501 tcttatacga atctcatttt taggtaatgc tgccaattta ctgatttagt gtgtgatggt
     4561 gattttaagg tgctccagtg gcttctgttt ctatcagctg cccctcctgt tccgctactg
     4621 acggggtggt gcgtaacggc aaaagcactg ccggacatca gcgctatctc tgctctcatt
     4681 gccgtaaaac atggcaacta cagttcactt ataccgcctc tcagcccggt acgcaccaga
     4741 aaatcattga tatggccatg aatggcgtcg gatgtcgcgc cagttcacgc attatgggcg
     4801 ttggcctcaa cacggtttta cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg
     4861 catacaaccg ggcagtgatg tgattgtctg cgctgaaatg gacgaacagt ggggctacgt
     4921 cggtgctaaa tcacaccagc gctggctgtt ttacgcgtat gacaggatac ggaggacggt
     4981 tgtggcgcac gtcttcggtg aacgcattct ggccacactg gagcgtcttc tgagcccgct
     5041 gtcggccttt aaggtcgtgg tatggatgac ggatggctgg ccgctttatg aaccccgtct
     5101 gaagggaaag ctgcacgtta tcagcaagcg ttacactcag cgcattgagc gacataacct
     5161 gaatctgaga caacatctgg caaggctggg acggaagtca ctgtcgttct caaaatcggt
     5221 ggagctgcat gacaaggtca tcgggcatta tctgaacata aaacactatc agtaagttgg
     5281 agtcattacc
//
