LOCUS       NZ_CP138782             4683 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 229 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP138782
VERSION     NZ_CP138782.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040200
            Assembly: GCF_033868475.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:01:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,620
            CDSs (total)                      :: 5,495
            Genes (coding)                    :: 5,374
            CDSs (with protein)               :: 5,374
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 51 of 121
            Pseudo Genes (incomplete)         :: 73 of 121
            Pseudo Genes (internal stop)      :: 21 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138782.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="229"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021"
                     /collected_by="He Fang"
     gene            join(4622..4683,1..826)
                     /locus_tag="R8557_RS28065"
                     /old_locus_tag="R8557_28065"
     CDS             join(4622..4683,1..826)
                     /locus_tag="R8557_RS28065"
                     /old_locus_tag="R8557_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_163535632.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKE
                     NRLNHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSLDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLIPSPLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     gene            823..1521
                     /locus_tag="R8557_RS28070"
                     /old_locus_tag="R8557_28070"
     CDS             823..1521
                     /locus_tag="R8557_RS28070"
                     /old_locus_tag="R8557_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159205176.1"
                     /translation="MTRTFFLLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNALI
                     QNHNLETAALRSHYSGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(1528..1932)
                     /locus_tag="R8557_RS28075"
                     /old_locus_tag="R8557_28075"
     CDS             complement(1528..1932)
                     /locus_tag="R8557_RS28075"
                     /old_locus_tag="R8557_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_163535629.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFAPVSVEQAEEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1932..2123)
                     /locus_tag="R8557_RS28080"
                     /old_locus_tag="R8557_28080"
     CDS             complement(1932..2123)
                     /locus_tag="R8557_RS28080"
                     /old_locus_tag="R8557_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2534..2647
                     /locus_tag="R8557_RS28085"
                     /old_locus_tag="R8557_28085"
     CDS             2534..2647
                     /locus_tag="R8557_RS28085"
                     /old_locus_tag="R8557_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            3135..3251
                     /locus_tag="R8557_RS28090"
                     /old_locus_tag="R8557_28090"
     CDS             3135..3251
                     /locus_tag="R8557_RS28090"
                     /old_locus_tag="R8557_28090"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_016947047.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRTFRRRR"
                     /name="small membrane protein"
     gene            complement(3628..3828)
                     /locus_tag="R8557_RS28095"
                     /old_locus_tag="R8557_28095"
     CDS             complement(3628..3828)
                     /locus_tag="R8557_RS28095"
                     /old_locus_tag="R8557_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162092.1"
                     /translation="MKLITVDESVVNAIVEYYAQGSEEAHAEGNRMFLRYLHELTGTQR
                     VAVEAARKQSLAAINKGGASL"
                     /name="hypothetical protein"
     gene            4408..4629
                     /locus_tag="R8557_RS28100"
                     /old_locus_tag="R8557_28100"
     CDS             4408..4629
                     /locus_tag="R8557_RS28100"
                     /old_locus_tag="R8557_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947202.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     Typing          2172..2647
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.871"
                     /coverage="92.0"
                     /evalue="2.7e-180"
ORIGIN
        1 tattctgcgc tctgtcggca cagtccggaa ttacgagcag ggactgaccc gcgtcgcaga
       61 gtggatgaaa gagaaccggc tcaaccacgg cgatttacgc acactgacac cggcgcaggc
      121 catcacctac ctggagcagc gcggggagca ggtcggccag aaaacgctcg atatggaacg
      181 ccaggccatc caggccatga tgatccacgt gacgcagacc ctccctgccg gcgaacacct
      241 gccggtggtg aaatcggagc aggcacagat cctgagcagc cgggcctaca gcctggacca
      301 ggtagccctg attgccgcag cacaacagcc cgccaacgcc ctggccaccg cgattgccct
      361 ggccgccgga ttacgcgccc atgaactgct gaccctcgcg ccccgccagg agcgtgcacc
      421 ggatccccgt ccggcagcgg acagtaaatt tgccggtcgg gagggtgtga tttacaccgt
      481 tcagggcaaa ggcggcctgg tcagggaagt cctgataccg tcccccctgg ctgaacgtct
      541 cgagcatgtc aggctggcga gcccggtccg ggtgaccgat cgcggcgtgt tttaccagag
      601 tcagtacgcc attggcggag gacagcgctg gtcgaacgcc ttcagtgccg cctccatgcg
      661 taccctgggc tggtcacggg gggcacacgg cgtgcgtcac agctacgccc agcaacggat
      721 gcaggagctg caaaaactcg gtctggttcg tgatatggct ctgcgcaccg tcagccagga
      781 aatgggccat tttcgtccgg aaatcacgga gacatacctg cgatgacaag aacgtttttt
      841 ttattgtgta ccggtgtttt cgccatcgcg tacgccctgt atgagctgaa tcactatggc
      901 gtttactatc cggacgtcag caatcctgag gcgtttactg gctggaaggc ggtcaccgaa
      961 ctcggtcccg ctatcctggc aacggggatc gcagccacag cgttctcgct gtggctgttc
     1021 attttgtttt tcagaggctc agagtcccgc cggatacagc gcgagataag cggatatcag
     1081 caggcactga gggaggcaga agaggctcac agagccagac aaaatgccct tattcagaat
     1141 cacaacctgg agacagcggc cctgcgaagc cactacagtg gtgctgagga tgtactgcgg
     1201 gaagcgtttg cgcaaaaaga aagcctcttg cttcaggcgg tgcagaatgc ccggaaagaa
     1261 gccagaaagg agctggaaga acagagggag gcactgaatc agcgcgaatc catcctgcag
     1321 aaaaaggagc tggcgctgga aacaaccctg gagaaggccc ggaaatatgt ggccggggag
     1381 cggcataaca ccgtgctggc ccgtctggaa gcagagaaag cagaggaaag acggagaaat
     1441 gcctgcgcga cggcggaacg gcgcagacgg aaactggaaa aactccaggc cactggcgga
     1501 acagcagggc aaaaacactg aggcatttca ggactgccag aaacgccacc aggcacgggg
     1561 ccgggtcgtg ttctgccgct tctcttcccg gttctccagc aacaacatcg cctgtttcag
     1621 gctgtcgata tgtgaatctc tggaggcaag gagggtgttc aggtgttcaa tttcctgttt
     1681 cagaatgtaa atgacctgtt cattacggtc agaagtgact gtgtcacgct gtccactgac
     1741 ctgttcttcc gcctgttcaa cggataccgg cgcaaaggca ccaaacaccc gcaaaagctc
     1801 tgaagtatcg agcattttcg cattccgttc accccgacag gtggacagtt caccggcttt
     1861 aattaacctg tgcagggttg tcctgctctt acccgtcagg cgtgcagcct cactgatcga
     1921 cactttcgcc atcagtcacc ctcctgtagt gcatccagtc tgagggacag tgtacggaaa
     1981 agatgctccg catcatcgtg gagtttctca caaaggtcag cctccgcatc gagatcaagc
     2041 tcgttaagct tctccagcag gagcagggac tgattctgga tgaatttagc catgttcagc
     2101 gcagttttct gttgtttcgt cataaggggc ctcagggcgt cagtgctcaa tgtcagacgg
     2161 ggctcttttc cgcttcctcg ctcactcagt cgctacgctc ctgccgttcg gctgcggcga
     2221 gcggacggga cacacagaag agtcatcgct atcaccagga agagaagagc ctgtggaaaa
     2281 agccacggcg ttgccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     2341 acgctcaaat cagtggtggc gaaacccgac aggacttaaa gataccaggc gtttccccct
     2401 ggtggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     2461 ttatggccgc gtttatctca ttccacgcct gacactcagt tccgggaagg cagttcgctc
     2521 caagctggac tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct tatccggtaa
     2581 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagcg ccactggcag cagccactgg
     2641 taactgattt agaggagata gtcttgaagt catgcgccag ttaaggctaa actaaacgaa
     2701 ctgttttggt gactgcgctc ctctaactag ccagttacct cggttcaaag agttgctgct
     2761 ctgagaacct tcgagaaaac cgccctgcaa ggcggttttt tcgtttacag agcaagagat
     2821 tacgacgaga ctgaatgaat ctcaagattt catttagcct gccacccgat cattcaaagt
     2881 tttggcgtag ttctgacccg ctccccgtga tttgttacat acccaccaca gggcattagt
     2941 tttttttatt tttttgatga tatgtacaaa atcagtggtt aggaataatc ttagcagcct
     3001 taacaaacac atacaaaaac agcggttatg tttcatctca ccacaataag taacgacagt
     3061 atcatgctaa aaaaagatga tattatcttt atgaaatcat aacaggctgt ttttattaaa
     3121 ttaatcaggc atttatggac tggatttcac ttgctatcgc cattgctcta cttgttgtag
     3181 ctgtctactc aggaatctca tacttcagag aaaaaaaaac aatcagtcgt acatttaggc
     3241 gtaggcgata gcgccctatt taccataatc ccttcttacg cgcaccgcag aaacggcctc
     3301 agggcgtttc tggtggttaa cgggcaaaag gtggtggcaa gtacaccgaa aaccgccaga
     3361 aacgcgcctg atttatttta cgcatatgca tggctatgca caccaccgca tagccggtga
     3421 tttcggccga gaaaagggcc ccggacgtta aaagcataaa agaacgggcc atccaggccc
     3481 gtcaaatgag gacaactctg taaaaggacc atcgcacctg gtgcggtgcc ggcgacaaga
     3541 aaaagatagc tcatatcagc ccctcaccgg agaaaaatat ttccgggcca gtgcacagac
     3601 atcagccgtc cataaatgcg attttaatca cagggaagcg ccccccttat ttatcgcggc
     3661 cagagactgc tttctggccg cctcaaccgc cacacgctgt gttcccgtta actcgtgcag
     3721 ataacgcagg aacatcctgt ttccctctgc atgcgcctcc tcactgccct gtgcgtagta
     3781 ttccactatc gcgttcacca cggattcatc caccgtaatc agcttcattc tgtctctccg
     3841 ctttctctgt gacaccatcc tggcatcacg acggtcatcc tggcgtgaaa acggtgaccg
     3901 ggcaggcaaa ttttttgtct gtccggtcac cgttggcggg gtccggggcg gtcagtcccg
     3961 gtgtttttta ttcaggcaat atgagcacct gcgacacccg ctatgtacga cgcatgaatg
     4021 cgcctgtaca tagcgggtgt cgctattgag cggagtcagg cgcaacattt aaaaaacaac
     4081 attcacacgc acgaaaaacc tggctagtat ttagtcaaac catgaggagg cgcactctga
     4141 cgcgcacctg cctaccggca gcaggtaaaa cagccgggtc cctccgggga tactgtaccg
     4201 ggcagcccaa tccgagaatc ctgagaaaac agctaaaatc tgcgagaccg gcaatcaggt
     4261 caggccattc cctggcttaa gggacgttat cagccgcgag cagtccgggc tgaaccacga
     4321 gaggttcgaa cgagggcgat cggcgcatgc cgcagtgacc gttaacgtaa cggccactga
     4381 tgttcttaca cccaatccgg agcacagatg caatttgata ccgctgccga actggccgcc
     4441 ctgcaggcgc agacccgccg tatccgccag gtccgctacc gtccctcccg ccttgaccgc
     4501 tataccggcg aactgctgag cctgtaccag gccggggcca gtgcagcgga gctgcaacgc
     4561 tggctccggg cccggcgaat caaggtggtg ttgtccacgg ttacccgctg gctggaaaaa
     4621 aatggctaaa ttcgccagcg ctgcccgcca ggcagctggc gtgataaaac cactccaggg
     4681 aaa
//
