LOCUS       NZ_CP138778             2352 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 110 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP138778
VERSION     NZ_CP138778.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040198
            Assembly: GCF_033867515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:06:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,380
            CDSs (total)                      :: 5,254
            Genes (coding)                    :: 5,098
            CDSs (with protein)               :: 5,098
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 96 of 156
            Pseudo Genes (internal stop)      :: 28 of 156
            Pseudo Genes (multiple problems)  :: 28 of 156
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138778.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="110"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-06"
                     /collected_by="He Fang"
     gene            complement(join(2234..2352,1..613))
                     /locus_tag="R8536_RS26895"
                     /old_locus_tag="R8536_26895"
     CDS             complement(join(2234..2352,1..613))
                     /locus_tag="R8536_RS26895"
                     /old_locus_tag="R8536_26895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_277175075.1"
                     /translation="MGNSKRNINKLNENFREDILDYAIAHNLKCANALAILYATGCRPD
                     ELHTGVKINYDKQKNEIRFKIIGSKLNRRMKRGIGIREFSVKINNDNERFFKGIIDLIN
                     ERPVDSFDHNFKIESAKAFSGYITKISKKLWPRKTYHASAYSFRHAKATELKNSDYDKE
                     KIAQIMGHASVRSQQSYGRKNKKSKGGFNDIEDIKTNIKPRGADRLLRFKIASKNQAAA
                     KIADTSTPSSPPPAPVRRFKM"
                     /name="site-specific integrase"
     gene            complement(982..1728)
                     /locus_tag="R8536_RS26900"
                     /old_locus_tag="R8536_26900"
     CDS             complement(982..1728)
                     /locus_tag="R8536_RS26900"
                     /old_locus_tag="R8536_26900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_194967212.1"
                     /translation="MDIGNILNESLSIDYEKLDLFLEKYDLTPEQKVAVYEFHAKAYKK
                     NKTLVISETKENKFKSISEGVEYVHLFPKNLKILIRKYGLNTNELLVLTEIMESMLSHG
                     NLLINFSQKALCELTGINKSTMCKTFKTLKQKQCLIEKNGHIYLNSVIFMKGLPHKLFM
                     QFRDHFLNSISYKLDDEEEFEKVFDDNFIKAYEKNLKEIKKKKQQIKEKKISKALDNFE
                     KEISKEWKEKFKDEEENFEFGFESEI"
                     /name="hypothetical protein"
     Typing          complement(1991..2174)
                     /name="Col(IRGK)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.624"
                     /coverage="99.0"
                     /evalue="1.28e-74"
ORIGIN
        1 cacggggttt aatattagtt tttatatctt caatgtcgtt aaacccacct ttgctttttt
       61 tgttttttct accgtaactt tgctgagatc taactgacgc gtggcccatt atttgggcta
      121 ttttttcttt atcataatct gaatttttca actcagttgc ttttgcatgt ctgaaagaat
      181 atgcagaagc atgataagtt tttcggggcc ataacttttt gcttattttt gtaatatatc
      241 cggaaaatgc ctttgcactt tcaattttaa aattatgatc gaatgaatca acaggtcttt
      301 catttattag atcaataatt cctttaaaaa atctttcatt atcattattg atttttactg
      361 aaaactcacg aattccaata cctcttttca ttcttcgatt aagttttgat ccaataattt
      421 taaatctaat ttcatttttt tgtttatcgt aatttatttt aaccccagtg tggagttcgt
      481 ccggacggca accggtggcg tataaaatag cgagtgcgtt agcacatttt agattgtgcg
      541 cgatcgcata atctaaaata tcctctctaa aattttcatt taatttattt atatttcttt
      601 ttgaattacc cataatagtt tttcctcatt gcaaaattta aagaaccgga cattttaaaa
      661 attcgctttg ctcatattta aaataccgta aaacccaaac cctcccccct tatgttctat
      721 ttttcgctgt cgctcaaaat cgaacaacga cccccggttg ggggtggcag ttttacgggt
      781 tcgcttcgct ttacccctaa aatgccttgt tgttccaccc aaaaagacgg gttgaaacaa
      841 caacttatac tactgttata tccgggtgga agttaatgtc aatattgcaa atgtggagaa
      901 acaatgaata aaaaaaatcc cttagtggaa tagaatttat tctatttcaa taagggattc
      961 gcctgcccgg cgagggcggt tttatatttc cgattcaaaa ccaaattcga aattttcctc
     1021 ttcgtcttta aacttttcct tccattcttt cgagatttct ttttcaaaat tatctaatgc
     1081 ttttgatatt ttcttttctt ttatttgttg ctttttcttt ttaatctctt tgagattttt
     1141 ttcgtatgct ttaataaaat tatcgtcgaa gactttttca aattcttctt catcatctaa
     1201 tttatatgag atagaattta aaaaatgatc tctaaattgc ataaacaatt tatgaggtaa
     1261 ccctttcata aatatcacag aatttaaata aatatgtccg tttttctcaa ttaaacactg
     1321 cttttgtttg agggttttaa atgttttaca cattgtagat ttattaattc ctgttaattc
     1381 gcaaagtgcc ttttgcgaaa aattaattaa taaatttccg tgtgaaagca ttgactccat
     1441 tatttccgtt aaaaccaata attcgtttgt atttaaacca tattttctaa ttaaaatttt
     1501 taaatttttt gggaataaat gcacgtattc aacaccttcg gaaatagatt taaatttatt
     1561 ttcttttgtt tcagaaataa ctaaagtttt atttttttta taagcttttg cgtgaaattc
     1621 ataaactgca actttttgtt ctggtgttaa atcatatttt tccaaaaaca aatctaattt
     1681 ttcgtaatca atacttaaac tctcatttaa tatatttcca atatccatag aaacctcctc
     1741 tttttattta tttctatata tatgttttaa catttataaa ggactagtca atagattaag
     1801 ttgcaaaaag tagaaaaagt tgcagtttag aaactgtagt ttctacttgg aaactggcta
     1861 gagttgtcgg atttgacaac ctcagttgac gaatttgaca actggagttg acgaatttga
     1921 caactacggt tgaagttttt gacaactgcc atttttaaaa aatgagtaat atcaatggtt
     1981 agagagtaat tttttaactc ctaagaacat ctaagaattt taagaacact aagggggtga
     2041 aattttcaac ttttgagatg gtttttaaga tcattggttt tcaagtaaca gatcaatccc
     2101 ccacagggga aaaccatcca gttgcccggt tccgggccct gcttcgcaat atggaggatt
     2161 ttgctagaaa accggctcgc cgccaggcca acccttcaca gtccaggagg ggacctcctg
     2221 aactgctcac ggttcacatt ttgaagcgac gaacgggagc cggtggagga ctgctggggg
     2281 tggaagtatc ggcaattttt gccgctgctt ggtttttgct ggcaattttg aaccttagca
     2341 atctatctgc ac
//
