LOCUS       NZ_CP138777             4822 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 110 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP138777
VERSION     NZ_CP138777.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040198
            Assembly: GCF_033867515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:06:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,380
            CDSs (total)                      :: 5,254
            Genes (coding)                    :: 5,098
            CDSs (with protein)               :: 5,098
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 62 of 156
            Pseudo Genes (incomplete)         :: 96 of 156
            Pseudo Genes (internal stop)      :: 28 of 156
            Pseudo Genes (multiple problems)  :: 28 of 156
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138777.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="110"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-06"
                     /collected_by="He Fang"
     gene            66..257
                     /locus_tag="R8536_RS26855"
                     /old_locus_tag="R8536_26855"
     CDS             66..257
                     /locus_tag="R8536_RS26855"
                     /old_locus_tag="R8536_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            257..658
                     /locus_tag="R8536_RS26860"
                     /old_locus_tag="R8536_26860"
     CDS             257..658
                     /locus_tag="R8536_RS26860"
                     /old_locus_tag="R8536_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064175096.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1386..1649
                     /locus_tag="R8536_RS26865"
                     /old_locus_tag="R8536_26865"
     CDS             1386..1649
                     /locus_tag="R8536_RS26865"
                     /old_locus_tag="R8536_26865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290305.1"
                     /translation="MVNLNDICRSKKAAWIGGFFIFLARQNGIFDTNSGWLLLRVQFLS
                     RTVLRAVLLYLQLLRYSMGMYEADRGFQTFFITPFFYQQTWI"
                     /name="hypothetical protein"
     gene            complement(2380..2643)
                     /locus_tag="R8536_RS26870"
                     /old_locus_tag="R8536_26870"
     CDS             complement(2380..2643)
                     /locus_tag="R8536_RS26870"
                     /old_locus_tag="R8536_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_072072086.1"
                     /translation="MAAHTSETVKFLQSVSSDQTWWKSRYNPGDSVPYSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            complement(2713..2841)
                     /locus_tag="R8536_RS26875"
                     /old_locus_tag="R8536_26875"
     CDS             complement(2713..2841)
                     /locus_tag="R8536_RS26875"
                     /old_locus_tag="R8536_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072201952.1"
                     /translation="MSLKPGPKRIADSTGQPDRRQRDNKKTPGNTDKLKPSKSSSK"
                     /name="hypothetical protein"
     gene            complement(2897..3265)
                     /locus_tag="R8536_RS26880"
                     /old_locus_tag="R8536_26880"
     CDS             complement(2897..3265)
                     /locus_tag="R8536_RS26880"
                     /old_locus_tag="R8536_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290306.1"
                     /translation="MKTTSEFARDAQKWDDRELGASENHVEVASMADMSAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAERKQALRRELDKLNEREEIHQ
                     QEDTVPVDEFIESIRKQA"
                     /name="hypothetical protein"
     gene            complement(3462..3755)
                     /locus_tag="R8536_RS26885"
                     /old_locus_tag="R8536_26885"
     CDS             complement(3462..3755)
                     /locus_tag="R8536_RS26885"
                     /old_locus_tag="R8536_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290307.1"
                     /translation="MTIVISKEIEEKLTTKHKVTKKEVHESLANLNGNLLIDNREQHKT
                     NPPTNWFIAETNQRRELKVCFMIIDGDIHIKTAFEPNEIERKIYATHGKKTT"
                     /name="hypothetical protein"
     gene            complement(4364..4477)
                     /locus_tag="R8536_RS26890"
                     /old_locus_tag="R8536_26890"
     CDS             complement(4364..4477)
                     /locus_tag="R8536_RS26890"
                     /old_locus_tag="R8536_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          complement(4365..4718)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.91"
                     /coverage="87.0"
                     /evalue="1.28e-167"
ORIGIN
        1 ggagtgagcg aggaagcgga aaagagaaca tccctacact gagcactgac gccctgaggc
       61 cccttatgac gaaacaacag aaaactgccc tgaacatggc taaattcatc cagaatcagt
      121 ctctgctcct gctggagaag cttaacgagc ttgatctcga tgtcgaggcc gacctttgtg
      181 agaaactcca cgatgatgcg gagcatcttt tccgtacact gtcctcgaga ctggatgcat
      241 tacaggatgg taactgatgg ccagagtgtc tattagtgag gctgcccgac tgacgggtaa
      301 gagcaggaca acactgcaca ggttaattaa aacaggtgac ctgtccacct gtagtggtga
      361 gcgcaatgca aaaatggtag atacttcaga gcttttacgt gtctttggtc cctttgagca
      421 accgaagggt gaacaccatt ctggacaggt ctctgaacag cgtgatacag cctctgctga
      481 ccagagtgaa caggtgattc gtcatctgaa acaggagatt gaacacctga acaccttact
      541 tttgtccaag gattcgcaca ttgacagcct gaaacaggcc atgctccttc ttgaaaatca
      601 ccaggcaaaa ttaactgaat ccccgacgcc atggtggaaa ttctggaaaa aatcctgacc
      661 ccgtcatgct tcagttttcc tctccggcca tcccggggag ttcctgaacc ttcccaaaaa
      721 tcgtcagatc tctgaacgct cctcaccact aaaaataccc acaccccacg caaaattaaa
      781 attttgcaga ttccttattt acaacaatgg gttaatgaaa aataaatgtg cggtgatgtg
      841 ttacaaagtg cagatccaag tcgtggacaa ctctatggcg aatcgctgac gaattgccca
      901 cgattcatat gtgaatcacg ctttattatc atattgttat gatgaatcga ctcttgattc
      961 aattcatcac tctgggtttt ttttgttcat ggtgactctt gattcaattc acgattcgaa
     1021 tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat tgccggccgt
     1081 cgacgaaccc gattcctgaa cgtgctgaaa accggttttc cggctgcgcc ggcaacgctt
     1141 cacggccacg caataaattg cgtccccgtt caccgttttc ggataggaag aacgccggaa
     1201 acaggactta ccctggctga atggcgtgaa ttttcgcgct atgcacttgc gtgcatactc
     1261 atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc
     1321 tgttttaccg gtttcccgtc agaaacgcct tgaggccgtt tttgcggtgc gtacaagaag
     1381 acgttatggt aaatctaaat gatatctgca gaagcaaaaa agccgcctgg ataggcggct
     1441 ttttcatttt tttggcgcgg cagaacggca ttttcgacac caactctggt tggttgctcc
     1501 tgagggtaca gttcttaagc cgaacagtgc tgagagctgt cctgctgtat ttgcagttgt
     1561 tacgttacag catgggcatg tatgaggcag accgaggttt ccagacattc ttcatcactc
     1621 cattttttta ccaacaaacg tggatatgac tgcacccaag gcgatcatgc ctaatgtgcc
     1681 gactccgccg ccaatacctg atgtacatga cggacacaac tcatcgtcat acaaacgacc
     1741 acccttagcg taattctcac accccggagc gcgacatatg taaattgctt ctttgcactc
     1801 agggcattcg cacttatctc gtcgtacatg gttttcttga attacacgag gtataacttc
     1861 agctttacaa aaaggacata accctttgtg ttttgatgtc attttgacct acttaattgc
     1921 actgcaacta attaattttg aacgacattt agggcagtag ttcggtgttc caaacccact
     1981 cctactagtc cattcatgcc gacaagccat gcatttagcc cgatagataa caacaaccgc
     2041 catgaaaaac caccattaat atcagtaact aatattcatt tataataact gacaaactaa
     2101 acaagtaaag taaattgtgt atttttttaa gtgattgatt tttaatttat ttttctatta
     2161 tctaaaggct gtaaatttta attaatgcat taaatacaat gacttaatct gatttgtttt
     2221 ttttgtttaa cttttttgga gggcgagaaa gactcatgca tctcgttcgt gcgctcatgc
     2281 agcgtaacga ttgttagcat acaagaagtg cggggctggg atgttatggt aaatatcttc
     2341 gagcttcagg tgcaaaaaaa agccccggag ggctctcaat catttgattc cgaaacggtc
     2401 acctttagtg tccgttctga caatcaattg ccattttatt gcttgttgct gactgtgctg
     2461 atggtggttt tgtggtggga acgggtcgct gccgttactg gtgatctctt ttccgcaaac
     2521 tgtacatcga taaattccag aatagggaac gctgtctcct ggattatatc gtgacttcca
     2581 ccatgtttga tcgctactga cgctttgtaa aaacttcact gtttcgctgg tatgtgcagc
     2641 catttagctt tccttttctg aatttatagg actgacaatg aaaagccgcc taaaaggcgg
     2701 cttttaactg attcatttac ttgaggattt actgggtttt aatttatcag tatttcccgg
     2761 tgttttttta ttgtcacgtt ggcgtctatc aggttgccct gttgaatctg cgattctttt
     2821 tggaccaggt ttaagactca tgataatcct cctttcaaac gcgatgaaat tttttctatc
     2881 tagatggtaa gctctttcat gcttgttttc ttatgctttc aatgaattca tcaactggaa
     2941 ctgtgtcctc ctgctgatgt atttcttctc gttcattgag cttgtccaac tctctacgta
     3001 atgcttgttt tctttctgca ataacaaatc gttgcagaag gtctctaacc attggttgat
     3061 aacctatacc atagctttca gctatcgatt ttaaatcgct tacaagattt ttctgtaatc
     3121 ttattgagat ggcttgcagt gatagtgcat cgttgaaagc tgacatgtca gccatcgatg
     3181 ctacttctac gtgattctca gaggcgccga gctctcgatc atcccatttt tgtgcatcac
     3241 gtgcgaattc actggtagtt ttcattgtat agacctctag gtgctgtttc ctgcagatca
     3301 ggattaactg caatatttat gctttatcta gttccgaaag gaacttctat gttgagaaat
     3361 caggttcagc ttcattttca ttttagttct ttcgctgatg taagattttt gcaaggtctt
     3421 acatggtaag gccttgcaaa acaattactt atttagcgct gttatgtcgt cttttttcca
     3481 tgcgtagcat agattttacg ttctatttcg ttcggttcga aggctgtttt gatatgaatg
     3541 tcgccatcga taatcatgaa acacactttc aactctctac gctggttagt ttcagcgata
     3601 aaccagttgg ttggaggatt ggttttgtgt tgttctctgt tatctatcaa caggttaccg
     3661 ttaaggtttg caaggctttc atgtacttct tttttagtta ctttatgttt tgtggttagt
     3721 ttttcttcaa tttctttact gattacaatt gtcatatttc atgatgcctc ggcctttgtt
     3781 gtgaaattga attcattgta tatacaaaat ttacatttgt caatccatgg cttgattgtt
     3841 aaggtgtttt cattttctat gtgtatatac ataggctgtt tcgattgctt cttcgcagcc
     3901 tttggtccgt tgatattggt tatgaaagat agatatagaa gaagttatga gcaatacaat
     3961 agcattgcct cattcatgtc attgttctga ctatgattat cgattgaacc cagtcgtagc
     4021 tttgacctat caagaggttg acgctctatt tggttttaga aatctacgcg gcaacgtagc
     4081 tcctcagacg tggtgtagag tttgtcgtgt taaaggtgct gcagatagca aaaagaaaaa
     4141 ggcacaatct taggctgagg atcttcttga gatcatgtgt tagtcgtcgt aatctcactg
     4201 ctgtgaaaac gaaaaaaccg ccctgcaagg cggttttttc gaaggttctc tgagctacca
     4261 actctttgaa ccgaggtaac tggcttggag gagcacagtc accaaaactg ttcgtttagt
     4321 ttagccttaa ccggcgcata agttcaagac tatctcctct aaatcagtta ccagtggctg
     4381 ctgccagtgg cgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata
     4441 aggcgcagca gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     4501 ccttcccgga actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg
     4561 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac
     4621 gcctggtatc tttaagtcct gtcgggtttc gccaccgctg atttgagcgt ccgtttctgt
     4681 gatgcttgtc aggggggcgg agcctatgga aaaacggcaa cgccgtggcc ttttccacag
     4741 gctcttttct tcctggttat cgcgatgact cttctgtgtg tcccgtccgc tcgccgcagc
     4801 cgaacggcag gagcgtagcg ac
//
