LOCUS       NZ_CP138762             4573 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella oxytoca strain 86 plasmid unnamed5, complete sequence.
ACCESSION   NZ_CP138762
VERSION     NZ_CP138762.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040195
            Assembly: GCF_033864495.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:44:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,894
            CDSs (total)                      :: 5,778
            Genes (coding)                    :: 5,675
            CDSs (with protein)               :: 5,675
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 33 of 103
            Pseudo Genes (incomplete)         :: 69 of 103
            Pseudo Genes (internal stop)      :: 15 of 103
            Pseudo Genes (multiple problems)  :: 12 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138762.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="86"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021"
                     /collected_by="He Fang"
     gene            530..592
                     /locus_tag="R8547_RS29440"
                     /old_locus_tag="R8547_29440"
     CDS             530..592
                     /locus_tag="R8547_RS29440"
                     /old_locus_tag="R8547_29440"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319794694.1"
                     /translation="MYSGISYFREKKSISRAFRR"
                     /name="hypothetical protein"
     gene            1647..1868
                     /locus_tag="R8547_RS29445"
                     /old_locus_tag="R8547_29445"
     CDS             1647..1868
                     /locus_tag="R8547_RS29445"
                     /old_locus_tag="R8547_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947202.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            1861..2748
                     /locus_tag="R8547_RS29450"
                     /old_locus_tag="R8547_29450"
     CDS             1861..2748
                     /locus_tag="R8547_RS29450"
                     /old_locus_tag="R8547_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_048290304.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            2745..3443
                     /locus_tag="R8547_RS29455"
                     /old_locus_tag="R8547_29455"
     CDS             2745..3443
                     /locus_tag="R8547_RS29455"
                     /old_locus_tag="R8547_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290303.1"
                     /translation="MTRTFILLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYIGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTELARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(3450..3848)
                     /locus_tag="R8547_RS29460"
                     /old_locus_tag="R8547_29460"
     CDS             complement(3450..3848)
                     /locus_tag="R8547_RS29460"
                     /old_locus_tag="R8547_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_121576903.1"
                     /translation="MATVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFASVSVEQEEEQVSGQRDTVTSDHNEQVIHTLKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREDQRQNTTRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3848..4039)
                     /locus_tag="R8547_RS29465"
                     /old_locus_tag="R8547_29465"
     CDS             complement(3848..4039)
                     /locus_tag="R8547_RS29465"
                     /old_locus_tag="R8547_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4450..4563
                     /locus_tag="R8547_RS29470"
                     /old_locus_tag="R8547_29470"
     CDS             4450..4563
                     /locus_tag="R8547_RS29470"
                     /old_locus_tag="R8547_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          4088..4563
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.852"
                     /coverage="92.0"
                     /evalue="2.7e-180"
ORIGIN
        1 atagtcttga acttatgcgc cggttaaggc taaactaaac gaacagtttt ggtgactgcg
       61 ctcctctaac tagccagtta cctcggttca aagagttgct gctctgagaa ccttcgagaa
      121 aaccgccttg ctgggcggtt ttttcgttta cagagcaaga gattacgacg aggctgaatg
      181 aatctcaaga tttcatttag tctgcccccc gatcattcaa agttttggcg tagttctgac
      241 ccgccccccg tgatttgtta tatacccacc acgggggcat tagttttttt tatttttttt
      301 tttatgatat gtacaaaacc agtggttaag aataatctta gcagccttaa caaaacacat
      361 acaaaaacag cggttatgtt tcatctcacc acaataagta acgacagtat catgctaaaa
      421 aaagatgata ttatctttat gaaatcataa caggtttttt tattaaatta atcaggtact
      481 tattgactgt gttttacttg ctatcgccat tgctctactt attgtagcta tctactcagg
      541 aatctcatac ttcagagaaa aaaaatcaat cagtcgtgca tttaggcgat agcgccctat
      601 ttaccataat cccttcttac gcgcaccgcg aaaacggcct cagggcgttt ctggtggtta
      661 acgggcaaaa ggtggtggca agtacaccga aaaccgccag aaacgcgcct gatttatttt
      721 acgcatatgc atggctatgc acaccaccgc atagccggtg atttcggccg agaaaagggc
      781 cccggacgtt aaaagcataa aagaacgggc catccaggcc cgtcaaatga ggacaacact
      841 gtaaaaggac cgtcgcacct ggtgcggtgc cggcgacaag aaaaagatag ctcatatcag
      901 cccctcaccg gagaaaaata tttcctggcc acctcaaccg ccacacgctg tgttcccgtt
      961 aactcgcgca gataacgcag gaacatcctg ttttcctctg catgcgcctc ctcactgccc
     1021 tgtgcgtggt attccactat cgcgttcacc acggactcac cgacctgcat cgtctgacct
     1081 catctgcact ttcatcacac cgtttcagat cccgcctcct cgccatgatg tgaaacggtg
     1141 accgggcagg caaatttttt gtctgtccgg tcaccgttgg cggggtccgg ggcggtcagt
     1201 cccggtgttt tttattccgc caaatcagca cctgcgacac ccgctatgta cgacgcataa
     1261 atgcgcctgt acatagcggg tgtcgctatt gagcggagtc aggcgcaaca tttaaaaaac
     1321 aacattcaca cgcacgaaaa acctggctag tatttagtca aaccatgagg aggcgcactc
     1381 tgacgcgcac ctgcctaccg gcagcaggta aaacagccgg gtccctccgg ggatactgta
     1441 ccgggcagcc caatccgaga atcctgagaa aacagctaaa atctgcgaga ccggcaatca
     1501 ggtcaggcca ttccctggct taagggacgt tatcagccgc gagcagtccg ggctgaacca
     1561 cgagaggttc gaacgagggc gatcggcgca tgccgcagtg accgttaacg taacggccac
     1621 tgatgttctt acacaccgga gcacagatgc aatttgatac cgctgccgaa ctggccgccc
     1681 tgcaggcgca gacccgccgt atccgccagg tccgctaccg tccctcccgc cttgaccgct
     1741 ataccggcga actgctgagc ctgtaccagg ccggggccag tgcagcggag ctgcaacgct
     1801 ggctccgggc ccggcgaatc aaggtggtgt tgtccacggt tacccgctgg ctggaaaaaa
     1861 atggctaaat tcgccagcgc tgcccgccag gcggctggcg tgataaaacc actccaggga
     1921 aatattctgc gctctgtcgg cacagtccgg aattacgagc agggactgac ccgcgtcgca
     1981 gagtggatga aagccaaccg gattaatcac ggcgatttac gcacactgac accggcgcag
     2041 gccatcacct acctggagca gcgcggggag caggttggcc agaaaacgct cgatatggaa
     2101 cgccaggcca tccaggccat gatgatccac gtgacgcaga ccctccctgc cggcgaacac
     2161 ctgccggtgg tgaaatcgga gcaggcacag atcctgagca gccgggccta cagcccggac
     2221 caggtagccc tgattgccgc agcacaacag cccgccaacg ctctggccac cgcgattgcc
     2281 ctggccgccg gattacgcgc ccatgaactg ctgaccctcg cgccccgcca ggagcgagca
     2341 gcggatcccc gtccggcagc ggacagtaaa tttgccggtc gggagggtgt gatttacacc
     2401 gttcagggca aaggcggcct ggtcagggaa gttctgctgc cgtcacgcct ggctgaacgt
     2461 ctcgagcatg tcaggctggc cagcccggtc cgggtgaccg atcgcggcgt gttttaccag
     2521 agtcagtacg ccattggcgg aggccagcgc tggtcaaacg ccttcagtgc cgcctccatg
     2581 cgtaccctgg gctggtcacg gggggcacac ggcgtgcgtc acagctacgc ccagcaacgg
     2641 atgcaggagc tgcaaaaact cggtctggtt cgtgatatgg ccctgcgcac cgtcagccag
     2701 gaaatgggcc attttcgtcc ggaaatcacg gagacatacc tgcgatgaca agaacattta
     2761 ttttattgtg taccggtgtt ttcgccatcg cgtacgccct gtatgagctg aatcactatg
     2821 gcgtttacta tccggacgtc agcaatcctg aggcgtttac tggctggaag gcggtcaccg
     2881 aactcggtcc cgctatcctg gcaacgggga tcgcagccac agcgttctcg ctgtggctgt
     2941 tcattttgtt tttcagaggc tcagagtccc gccggataca gcgcgagata agcggatatc
     3001 agcaggcact gagggaggca gaagaggctc acagagccag acaaaatacc cttattcaga
     3061 atcacaacct ggagacagcg gccctgcgaa gccactacat tggtgctgag gatgtactgc
     3121 gggaagcgtt tgcgcaaaaa gaaagcctct tgcttcaggc ggtgcagaat gcccggaaag
     3181 aagccagaaa ggagctggaa gaacagaggg aggcactgaa tcagcgcgaa tccatcctgc
     3241 agaaaaagga gctggcgctg gaaacaaccc tggagaaggc ccggaaatat gtggccgggg
     3301 agcggcataa caccgagctg gcccgtctgg aagcagagaa agcagaggaa agacggagaa
     3361 atgcctgcgc gacggcggaa cggcgcagac ggaaactgga aaaactccag gccactggcg
     3421 gaacagcagg gcaaaaacac tgaggcattt caggactgcc agaaacgcca ccagggccgg
     3481 gtcgtgttct gccgctgatc ttcccggttc tccagcaaca acatcgcctg tttcagactg
     3541 tcgatatgtg aatcttttga ggcaagaagt gtgttcaggt gttcaatttc ctgtttcaga
     3601 gtatgaatga cctgttcatt atggtcagaa gtgactgtgt cacgctgtcc actgacctgt
     3661 tcttcctcct gttcaacgga tacggacgca aaggcaccaa acacccgcaa aagctctgaa
     3721 gtatcgagca ttttcgcatt ccgttcaccc cgacaggtgg acagctcacc ggctttaatt
     3781 aacctgtgca gggttgtcct gctcttaccc gtcaggcgtg cagcctcact gatcgacact
     3841 gtcgccatca gtcaccctcc tgtagtgcat ccagtctgag ggacagtgta cggaaaagat
     3901 gctccgcatc atcgtggagt ttctcacaaa ggtcagcctc cgcatcgaga tcaagctcgt
     3961 taagcttctc cagcaggagc agggactgat tctggatgaa tttagccatg ttcagggcag
     4021 ttttctgttg tttcgtcata aggggcctca gggcgtcagt gctcagtgta aaaccagtct
     4081 cttttccgct tcctcgctca ctcagtcgct acgctcctgc cgttcggctg cggcgagcgg
     4141 acgggacaca cagaagagtc atcgcgataa ccaggaagaa aagagcctgt ggaaaaggcc
     4201 acggcgttgc cgtttttcca taggctccgc ccccctgaca agcatcacag aaacggacgc
     4261 tcaaatcagc ggtggcgaaa cccgacagga cttaaagata ccaggcgttt ccccctggtg
     4321 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     4381 ggccgcgttt atctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     4441 ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgccttatc cggtaactat
     4501 cgtcttgagt ccaacccgga aagacatgca aaagcgccac tggcagcagc cactggtaac
     4561 tgatttagag gag
//
