LOCUS       NZ_CP138761             4863 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella oxytoca strain 86 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP138761
VERSION     NZ_CP138761.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040195
            Assembly: GCF_033864495.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:44:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,894
            CDSs (total)                      :: 5,778
            Genes (coding)                    :: 5,675
            CDSs (with protein)               :: 5,675
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 33 of 103
            Pseudo Genes (incomplete)         :: 69 of 103
            Pseudo Genes (internal stop)      :: 15 of 103
            Pseudo Genes (multiple problems)  :: 12 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138761.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="86"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021"
                     /collected_by="He Fang"
     gene            1187..2485
                     /locus_tag="R8547_RS29415"
                     /old_locus_tag="R8547_29415"
     CDS             1187..2485
                     /locus_tag="R8547_RS29415"
                     /old_locus_tag="R8547_29415"
                     /inference="COORDINATES: protein motif:HMM:NF024700.3"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_097414638.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGIIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            2558..2926
                     /locus_tag="R8547_RS29420"
                     /old_locus_tag="R8547_29420"
     CDS             2558..2926
                     /locus_tag="R8547_RS29420"
                     /old_locus_tag="R8547_29420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(3163..3276)
                     /locus_tag="R8547_RS29425"
                     /old_locus_tag="R8547_29425"
     CDS             complement(3163..3276)
                     /locus_tag="R8547_RS29425"
                     /old_locus_tag="R8547_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            3692..3883
                     /locus_tag="R8547_RS29430"
                     /old_locus_tag="R8547_29430"
     CDS             3692..3883
                     /locus_tag="R8547_RS29430"
                     /old_locus_tag="R8547_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045265536.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3883..4305
                     /locus_tag="R8547_RS29435"
                     /old_locus_tag="R8547_29435"
     CDS             3883..4305
                     /locus_tag="R8547_RS29435"
                     /old_locus_tag="R8547_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3164..3571)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 gctgaatggc gtgaattttc gcgctatgca cttgcgtgca tactcatgca tgccgtaaaa
       61 acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc
      121 ccgtcagaaa cgccctgagg ccgttttagc ggtgcgtaca attagggggt tatgttaaat
      181 ccacaccagc tacagacgta aaaaaagccc cgcagggctt tatatctata tatctgttct
      241 catccaatcc catgtgtctt gggtgagcca tcctgcgtat tctttggtta caccaatcac
      301 aagcaaggtg tcattattgt ccagatgcct acgcaaacga tccgacagtt gttgcgctgt
      361 ttctccggtt gatattagcc atgtggagtc taaagggtgg caccaccccg gcgatttttt
      421 taactcatca tatagtgcat tgtagttttg acctgcttga ttcaggtcat aagagacaca
      481 gtaaactgac atagaaccat cctctttaat caaatgtggg gcagcccgtt agggctgcag
      541 tcacaactta cttcttcttt tcacgattaa ttggaatttt gaacatccca ccgtttggac
      601 ggatgatccg tttgcccttt actgtgatat aagggcgaaa aatgtatgcc tgagactcgt
      661 ctgtctgcat ggtgcatttc ctatttttcg ccacatgagt gttgattttt gctatcaaaa
      721 ttgtactgta gcaaccaaca aatcttgtgg ctggctttat caagctcttg aggtggtact
      781 caagtgccgc atttgtgtat ggtttgttac gggttagttc tggtggtaca ggctaaccca
      841 cttacttcaa aaaacgtcgt ttttctcctt tcatgcgtca tttcctagat gtttcttcac
      901 tgtatcagct ccgctgagca taaatgctac aaagagaaac catccccatc ctgaaaccga
      961 gtgtaaagct agcactgctg agctgacaac tgcggctaaa ggaaccattt ggtatagaaa
     1021 aaatctcata gaccccctgc atttagcttt ttttaacaag tgttccataa cgtgctatct
     1081 attaaacgca cctgttgttt tagaacgctt gtatgctatc cttaagtcct gattttgtca
     1141 atatttactc attttgttgt ttaaggatgg cgtaaggggt gttcttatga tggttaattt
     1201 tagtctttct aacgcatttt cctttatgga tgctcaaaat atgagcatgt tggcggtgtc
     1261 atccgcgaaa gacgacataa acttggctaa cgtggtttca gtgagtgatg ttcggatact
     1321 gaaatctgct ctgatatttg gggctaacgc tagcgggaag tctaatctca tgaaaacaat
     1381 ttctttaatg aaagatattg ttttgcattc cgtgcaagct attgaaagta gcattacaaa
     1441 gaacgtaata ccgtttttgc ttgatgagga tggaccaaat aaaccatctg aaatggaaat
     1501 gacttttatt tcagatgggg ttaaatatag atatggattg tctatttttc aggggaaaat
     1561 aaaagaagaa tggttgtact acacaccaaa atcaagagaa accattctct ttgaaagaga
     1621 tggtctttct attgaggtaa ataaggcggc ttttagtgag tcaaaccttt ttatcaaaga
     1681 agggattatt caaaaaacaa gggaggatgt tccatttgtt tcggttgttg ctggttttaa
     1741 tggtgagcat tcaaaacgag tagttaattg gtttaacaat atcgtattcc tttctggttc
     1801 taatgaaggg atgtttacag caattaccat gaagttaata aatgagtctg aggattttaa
     1861 ggcttggcta aaaaaaatac ttccagcatt ccagattaat gatatttacc tcgatgaggt
     1921 cgcacctgat tttccatttg gaaatataaa aacagatgat gataatttaa aaaatttatt
     1981 tagttcaata caaaattttg caaaaaacca gaaggttttt tctcttagag tagtgaaaaa
     2041 aacagctagc ggtgagattt cagtacctat tgattttgag tctgagggta ctaaaaagat
     2101 agtttatctt cttggtccaa tctacaatgc catgataaac ggtaacttat tgttagttga
     2161 tgagtttgat tctaagtttc acaccctttt gtctaagttt ttgtttagaa tattccatgc
     2221 gcaaagcgag gggcttagcc aaataatagc tgctgttcag gatgttaatc taatgaacac
     2281 tgattgtttt agacgtgacc agatttggtt tgttgataaa gatataaaat caggaagtag
     2341 tcatctctat tctttggttg agtataaaga gaagcagcga actttaaaat catcgtatgg
     2401 gcatgattat ttatctggtg catttgatgc tatcccattg tttgataatt tagatgaatt
     2461 tgaaaaactt atgcctgagg gatgatttat gtcaaagaga ggccgcggtc ttcgtagctc
     2521 ttcgaggaaa gaggaaacaa aatcctcaaa gagcattatg aaaactacag atagatttat
     2581 ttctgttagt tttagatatt ttcataacgt cgatgatatt ggtcagtcta ttgattcttg
     2641 gagggttgac ggcagtttgc cagatttaat tgaaaagctt gtttatctta cagctaatgg
     2701 gataacaaaa gtcagctctg atggtatttt tactaactac actcgatttc cagattcagc
     2761 agttaatgat tttaaatgcc ctgatgaaat atcagttgat gagaactggg gtgttataaa
     2821 aaatatagga ggccagaaaa gaagggttgc tggtttttta aaggataatg tgttttatat
     2881 agtttttttt gataaggatc atcggttttg gaagagcagg aaataggctt agttacgatt
     2941 gtactgtatg ttgaggcaag atcttcttga gatcactgtg ttctcgtcgt aatctctcgc
     3001 tctgtaaacg aaaaaaccgc cttggcgggc ggttttttcg aaggttccaa gagttggcgc
     3061 tcttagaacc aaggtaactg gcttggagga gcgcagtaac caaaacttgt tctttcagtt
     3121 tagccttaac cggcgcataa cttcaagact aactcctcta aatcagttac cagtggctgc
     3181 tgccagtggc gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa
     3241 ggcgcagcag tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     3301 ctacccggaa ctgagtgtct ggcgtggaat gagataaacg cggccataac agcggaatgg
     3361 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcct atcagaaatg
     3421 ataggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt ccgtttctgt
     3481 gatgcttgtc aggggggcgg agcctatgga aaaacggccg ctgcgcggcc tcctcttttc
     3541 ctcctacctt gatcgctcgg ttttctcgag cttttataag aatggtcttg ccgctcgccg
     3601 cagccgaacg accggagcgt agcgactgag tgagcgagga agcggaaaag agactggttt
     3661 tacactgagc actgacgctc tgaggcttct tatgacgaaa caacagaaaa cagcgctgaa
     3721 catggctaaa ttcatccaga atcagtctct gctcctgctg gagaagctta acgagcttgc
     3781 tctcgatgtc gaggccgacc tttgtgagaa actccacgat gatgcggagc atcttttccg
     3841 tacactgtcc tcgagactgg atgaattaca ggatggtaac tgatggccag agtgtctatt
     3901 agtgaagcgg cacggcttgt taaggttagt cgccccacaa tttacaagat gattaacagt
     3961 gggaagttaa gttacacctc tgttgtaaag cacggtaagg ctattaaagt tattgatact
     4021 tcagagctta ttagagtgtt tggttctctt gatggtgtca tcgatactgt aaaatatgat
     4081 gtaaaaagtg acgcagaatc tacaggtgtt aacagcgttg gtttacatga tttacaacat
     4141 agaatcgctt tacttgaagc tgaaaacgat ggtcttaaag gggctgttaa ggctcgagat
     4201 gagcatattg attcccttcg tcaggctatg cagcttctgg agcacaagca cgaaccttct
     4261 tcaccacccc attccccgtg gtggaaattc tggaaaaaat cctgaccccg tcatgcttcc
     4321 gttttcctct ccggccatcc cggggagttc ctgaaccttc ccaaaaatcg tcagatctct
     4381 gaacgctcct caccactaaa aatacccaca ccccacgcaa aattaacatt ttgcagattt
     4441 gtatttaata tcagtgggtt actttcctga aatttcgttt tttaaatttt atttatatag
     4501 atccgactct atgacgagtc gtcatagagt ccccggcgag tcgtcataga gtcggtaacg
     4561 actcaggact ataaaaacaa taagttaata acgacccgac tctagacacg actccccgtt
     4621 tggttgtttt ttagacgcgg cgactctggg tacgactcta gtgaagattc gccggtgaat
     4681 cgtgtggtga gtcgtccggc gacttgccat aacgtgattg ccggccgtcg acgaacccga
     4741 tttctgaacg tgctgaaaac cggttttccg gctgcgccgg caacgcttca cggccacgca
     4801 ataaattgcg tccccgttca ccgttttcgg atgggaagaa cgccggaaac aggacttacc
     4861 ctg
//
