LOCUS       NZ_CP138760             4991 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella oxytoca strain 86 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP138760
VERSION     NZ_CP138760.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040195
            Assembly: GCF_033864495.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:44:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,894
            CDSs (total)                      :: 5,778
            Genes (coding)                    :: 5,675
            CDSs (with protein)               :: 5,675
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 33 of 103
            Pseudo Genes (incomplete)         :: 69 of 103
            Pseudo Genes (internal stop)      :: 15 of 103
            Pseudo Genes (multiple problems)  :: 12 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138760.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="86"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021"
                     /collected_by="He Fang"
     gene            complement(439..840)
                     /locus_tag="R8547_RS29380"
                     /old_locus_tag="R8547_29380"
     CDS             complement(439..840)
                     /locus_tag="R8547_RS29380"
                     /old_locus_tag="R8547_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060544629.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(840..1031)
                     /locus_tag="R8547_RS29385"
                     /old_locus_tag="R8547_29385"
     CDS             complement(840..1031)
                     /locus_tag="R8547_RS29385"
                     /old_locus_tag="R8547_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1447..1613
                     /locus_tag="R8547_RS29390"
                     /old_locus_tag="R8547_29390"
                     /pseudo=""
     CDS             1447..1613
                     /locus_tag="R8547_RS29390"
                     /old_locus_tag="R8547_29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1943..3001
                     /locus_tag="R8547_RS29395"
                     /old_locus_tag="R8547_29395"
     CDS             1943..3001
                     /locus_tag="R8547_RS29395"
                     /old_locus_tag="R8547_29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(3031..3288)
                     /locus_tag="R8547_RS29400"
                     /old_locus_tag="R8547_29400"
     CDS             complement(3031..3288)
                     /locus_tag="R8547_RS29400"
                     /old_locus_tag="R8547_29400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     gene            3706..4137
                     /locus_tag="R8547_RS29405"
                     /old_locus_tag="R8547_29405"
     CDS             3706..4137
                     /locus_tag="R8547_RS29405"
                     /old_locus_tag="R8547_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(4231..4635)
                     /locus_tag="R8547_RS29410"
                     /old_locus_tag="R8547_29410"
     CDS             complement(4231..4635)
                     /locus_tag="R8547_RS29410"
                     /old_locus_tag="R8547_29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     Typing          1153..1555
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="7.72e-165"
ORIGIN
        1 gaatcgggtt cgtcgacggc cggcaatcac gttatggcaa gtcgccggac gactcaccac
       61 acgattcacc ggcgactctg cactagagtc gtacctagag tcgccgcgac taaaaaacaa
      121 ccaaacgggg agtcgggtct agagtcgggt cgttattaac tcattgtttt tatagccctg
      181 agtcgttacc gactctatga cgactcgccg gggactctat gacgagtcgt catagagtcg
      241 gatctatata aataaaattt aaaaaacgaa atctcaggaa agtaaactac tgatattaaa
      301 tacaaatctg caaaatttta attttgcgtg gggtgtgggt atttttagtg gtgaggagcg
      361 ttcagagatc tgactatttt tgggaaggtt caggaactcc ccgggatggc cggagaggaa
      421 aactgaagca tgacagggtc aggatttttt ccagaatttc caccatggcg ccggggattc
      481 agttaatttt gcctggtgat tttcaagaag gagcatggcc tgtttcaggc tgtcaatgtg
      541 cgaatctttg gacaaaagta aagtgttcag gtgttcaatc tcctgtttca gatgacgaat
      601 cacctgttca cactggtcag cagaggctgt atcgcgctgt tcagagacct gtccagaatg
      661 gtgttcaccc ttcagttgtt caaagggacc aaagacacgt aaaagctctg aagtatctac
      721 catttttgca ttgcgctcac cgctacaggt ggacaggtca cctgctttaa ttaacctgtg
      781 cagtgttgtt ctactcttac ccgtcagtcg ggctgcctca ctaatagaca ctctggccat
      841 cagttaccat cctgtaatgc atccagtctc gaggacagtg tacggaaaag atgctccgca
      901 tcatcgtgga gtttctcaca aaggtcggcc tcgacatcaa gatcaagctc gttaagcttc
      961 tccagcagga gcagagattg atgctggatg aatttagcca tgttcagcgc tgttttctgt
     1021 tgtttcgtca taagaggcct cagagcgtca gtgctcagtg tagggatgtt ctcttttccg
     1081 cttcctcgct cactcagtcg ctacgctcct gccgttcggc tgcggcgagc ggcaggagca
     1141 tgcgtatgaa agctcgagaa aaccgagcga tcacgtaatt tagaaaaggg aaggccgcac
     1201 agcggccgtt tttccatagg ctccgccccc ctgacaagca tcacagaaac ggacgctcaa
     1261 atcagtggtg gcgaaacccg acaggactat aaagatatca ggcgtttccc cctgatggct
     1321 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     1381 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     1441 gactgtatgc acgaaccccc cgttcagtcc gactgctgcg cctgttccgg taactttcga
     1501 cttgagtcca acccggaagg cacgacaaaa cgccactggc agcagccact ggtaaggggt
     1561 gtacaagaat tactggtgca gagttcttga agttacagcc taactacagc taaactgaaa
     1621 ggacagattt ggtggcagcg gtcttgtaca ccctgttacc acggttcaga agttccccaa
     1681 cttactgatc cttcgaaaaa ccacctcccc aggtggtttt ttcgtttaca gagcaaaaga
     1741 ttacatcccg caggttagga tctcaagaag atccttttaa tcggaattcg acaactccag
     1801 ataagtcatg gtgagccagc caaccccttc gactgaatat cgttccaaaa aaaggcagtc
     1861 agctgaaaga aactaacagt agaacaataa tctctagtac cctgaatcta agctaacaac
     1921 aaaactagat taggagttat agatggcgat ctatgccgta tggaataaca agggtggtgt
     1981 agggaaaagt taccttactt ttcagttagc ctctgaatat gccagacaga accctactaa
     2041 aaaggtcctg gtggttgacc tgtgcccaca agcgaactcg tcctctatgt tacttggtgg
     2101 aatgataaat ggcgaaaaca cactaaacca gattcattca gcaatacaga gaaagacaat
     2161 atctggatat attgaggata ggatcagaag tccatatatt cctaccaaca ctggttctag
     2221 ctatgtaata aatgtatcta cacacaatag taaagttcca aataatctat atcttgtcac
     2281 tggcgatgaa caattagaac tccaagcatc aagagttgtt ggggcatcat ttccaggtcc
     2341 ccatgatgca tggcgtactg ttcacctctg gattaatgat ttaatcagtg acatttgcaa
     2401 ctcatggaat aatgaagata gctgcgtatt cattgactgc aaccctagct tctctattta
     2461 tacagagtta gctttaaccg cagcagagcg ccttttaatt cctttctctg ccgatggttc
     2521 atcaaagaga gcagttaagg ctgtactttc tttagtttat ggtgagcgtc gccatcagaa
     2581 tgaaccacaa tcagatttct atcttaatag caatcgttat tctatgtcgc taccacaaat
     2641 atatatgtat gttggaaata gattaacgca aatgaacaat tcatctgcca gtgcatttaa
     2701 gacggtagta accgaaattg gtgatgaaat ttggaaagta tggcaaacaa aaccaagtct
     2761 gttttgtatt cacccaaatg ggagctcaac gccaaataat aaaagatctt tcaggaatat
     2821 gtttcagtat gaagtaaatg atgcaaacac agcatctgtc gtatctggag ccttaggtat
     2881 tccaatcgcg acattaactg caggaaataa aactgtttca ggaaaaataa taaaggttaa
     2941 tcagacgcag ctagataaac aagtccccaa cattgtagct ctcgctggta tgatcgaata
     3001 aaatagcaag gcccttaaaa gggccttttt ttatttgttt ttctttactg cttgcagcgc
     3061 cctgccagca ctagaggcca actctttgat tacattatct ttactttcaa caacctcagt
     3121 aagcaactcg ttttcgtttt ccagtttatt caaagcgttc tgagtatctt caatagcatc
     3181 atcataccct tcggcatagc catccatcca accaagagta tacgtttggt ccaaaagatc
     3241 agcttgctta tcctgcagat ctttattctg attattgtta gagcccatgt tttttcctca
     3301 tttgttggcg catctgagtt ttcaaaccag gaggaattcc atttctaagt ttctcactta
     3361 gtctgactgg tggtgtgttt cctgagactg gattacagtc agcatccacc ccgacatctt
     3421 tctttttata gtcgccaata ttcctaacgt tatctgtagt catagaaaac cacgcccatt
     3481 gctgtgaata accgaactat aaaccagaaa aaaaacgtac ggcttatcat aacacttgac
     3541 acaaaaaaac accgctactc tccaagcaaa aaacataaaa ttggaaaaac aaaatcacaa
     3601 gcacatgata acaatagata ttaatattta cttacattta gtgagtttta taattagaaa
     3661 aacaatgtaa tttaataatc acaaacatta cctaaggacg aactgatgaa aatacccgcg
     3721 catttgaagc ataaaccaat tattcaagtc gaaaattacg accgcattga cggcccacat
     3781 gccgatgaca ctgatgctat ggggctatct gtaggtattg ctcaatggaa cactcctgga
     3841 tggactgagc tttcggcaaa ggtttggcga aacacaggag aaaaatggtc aagacaatca
     3901 gaagagctgc ctcttcaccg ggtaatcgac cttgcaactc tgatctgtat caccatggac
     3961 tacagtaaaa atgggcgact ttcatccagt gatgattttc ccgtgactcg ctcaactgat
     4021 aaccctgagc tagcacatca cactgagttt atgaaaaaag agttaaaaca aaataaaagc
     4081 aatcttgatg taagcctcaa acgcctctcg actgaattga agagacttgg ctattaaaac
     4141 tagctttaga aatgtctacc tacttcacag acgaatttac cataacatcc gtttttcgaa
     4201 ccataatggc aggctatagc ctgccgtaat tcaccggaaa aaatcatcca cagagtcgct
     4261 ccactcatgc ccggaaagag accacacctt gtttataggg ggaggataac cactcctgca
     4321 gtacccgttt tttcccacga aatcgaatgc cccgtgcggg tatccattga tcaggagtac
     4381 gcacttctga ctgtcttctg accagccaat tttaacatca gaaggaatgt gaccgtctga
     4441 aacgtcttcg gcattgtaga tgtgtactgc gtccataatc gggtttccct cgacggactc
     4501 atcgagcgca tagaaatacc cggtccggcc atcgtcttcg aacacggcgg ccagcatgcc
     4561 ctcaggagcg aggctcttta taacctgttc ctccccgaca acgagctggg cctgtatcgt
     4621 cactaaaatg gtcatcaggt ctgttctcct ttccttggtt gcttcggcag ccagctttca
     4681 gacaagcgga taatttacca taacatccgt tatcagcatc gtcagaaacg cgctgagggc
     4741 gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg ccagaaacgc
     4801 gcaggctctg tttttatggc atgcatgagt atgcacgcaa gtgcatagcg cgaatattca
     4861 cgccattcag acagggtaag tcctgtttcc ggcgttcttc ccatccgaaa acggtgaacg
     4921 gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa accggttttc
     4981 agcacgttca g
//
