LOCUS       NZ_CP138754             3360 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 071533A plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP138754
VERSION     NZ_CP138754.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040193
            Assembly: GCF_033870135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:40:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,518
            Genes (coding)                    :: 5,392
            CDSs (with protein)               :: 5,392
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 43 of 126
            Pseudo Genes (incomplete)         :: 81 of 126
            Pseudo Genes (internal stop)      :: 17 of 126
            Pseudo Genes (multiple problems)  :: 14 of 126
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138754.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="071533A"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed8"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-07"
                     /collected_by="He Fang"
     gene            complement(join(2995..3360,1..120))
                     /locus_tag="R8552_RS28170"
                     /old_locus_tag="R8552_28165"
     CDS             complement(join(2995..3360,1..120))
                     /locus_tag="R8552_RS28170"
                     /old_locus_tag="R8552_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096578.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<158..859)
                     /locus_tag="R8552_RS28175"
                     /old_locus_tag="R8552_28170"
                     /pseudo=""
     CDS             complement(<158..859)
                     /locus_tag="R8552_RS28175"
                     /old_locus_tag="R8552_28170"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(798..1118)
                     /locus_tag="R8552_RS28180"
                     /old_locus_tag="R8552_28175"
     CDS             complement(798..1118)
                     /locus_tag="R8552_RS28180"
                     /old_locus_tag="R8552_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2104..2265
                     /locus_tag="R8552_RS28185"
                     /old_locus_tag="R8552_28180"
     CDS             2104..2265
                     /locus_tag="R8552_RS28185"
                     /old_locus_tag="R8552_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane anchored protein"
                     /protein_id="WP_114263620.1"
                     /translation="MKKALLVLFTAMSVLVSMPSFAGNCQHDNDTAADGSRCGGRSADS
                     RPGGGGAR"
                     /name="transmembrane anchored protein"
     gene            complement(2779..2991)
                     /locus_tag="R8552_RS28190"
                     /old_locus_tag="R8552_28185"
     CDS             complement(2779..2991)
                     /locus_tag="R8552_RS28190"
                     /old_locus_tag="R8552_28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1227..1406)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.264"
                     /coverage="83.0"
                     /evalue="7.539999999999999e-48"
     Typing          complement(3..808)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.078"
                     /coverage="99.0"
                     /evalue="1.14e-158"
     Typing          complement(2664..3358)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="99.0"
                     /evalue="2.13e-142"
ORIGIN
        1 ttccgctttt acagacttct cgtgctcgct gaatgcggct ttcagcatct cgccggtact
       61 ctgctgctgc gctttcgatt gctgctccaa gtccttcgcc agcgctaaaa gactgctcat
      121 aaatggctcc cttcagtcgg atgtttcgcc ccccgtcagg gtcggcaatg ctgatactgt
      181 tttttgtagt gcggaccacc tcaaaacctg ccgctgtgag cgcccctgtg acgtcctcgc
      241 gcgttttgag ttccccggat gaggctaagg ccatcaaccc ccgtgtaatc gcctgagcgg
      301 cttccagctt cgtttccggc agtgcggacg gcgtgaccag taaccgccgg ttctcagggg
      361 cgtttggatc atgcagcccc agtctgccgt tcaccacggt ctgccaggca tcgatgcgcg
      421 gccggtcggc acgatcgtaa tacggctgca gacgtttccc ggtcagcagt tcagtgttgg
      481 ggatcaggaa attcagctcc agccgcccct tgtcggtgtg ctccacccac agaatgctgt
      541 actggtcttt atcgagcccg ggcatcagaa cccgctcaaa gctggccatg atctgctcac
      601 gctgcccggg cggcagttca gcctccgcga acgacagcac gccggaggtg tactttttcg
      661 cataaggcga ggcatcgata agctcccgga cttcctccgg ttttccctgc agcacggtag
      721 ccccttcgcg ctgccggtct tttcccagca ggtaatccac cggaccggca ccaccaccgc
      781 gtccccgggg atgaaattta acgatcatca tccccccctt tttccagcac cgcatgccgc
      841 agccgctcca gtcccgcatc gattgccatc agggcggcca caatctgcac ccggtcatga
      901 ccggcaccac ctgcattcac tttccgggcg atctggttca ggttgttacc cataccggca
      961 agctggcgca gcagcgccgg cgatatcgat ggcagtctgc cagcacgcgc cggcttctca
     1021 tcgaggcagg tctgacgcat ccacgccgcc agctgcttgc cgtcgcaccg ctcgagcagc
     1081 cgccggtgtt cgtcttccgt cacccacatc gtgagcatct tgttgcgttt atctgccaga
     1141 gtagcctcct gataacaccg ggacgggcgg gaaatttttc ccgggcggca cggtgttgga
     1201 caagccgcag gcgcgtcagt cgcttttagc gggtttcggg gcgcagccct gaaccagtca
     1261 cgtagcgcta gcggagtgta tactggctta atatggtaat tcgaagtggt aattcaggga
     1321 aatgattcat gtggcaggtg aaaaatgcgg catgtgggcg taagtgcaaa atgctggaaa
     1381 cgatgaactg acgcctcctc gctcactcgg tcgctgcgct cctgccgtga ggctccggcg
     1441 ggcgttgcgg ggatataact gagggataat gaaattttca ggctcaagaa gtacaaaagc
     1501 cccgtgacag aatcacgggg cttgagtact ctaattcttg cagatacctg ccaggatcag
     1561 acgtcacaat caaatcctag cagggctggc tagccttgtc actaccagct taataccatt
     1621 caacgacatt aaacgcatat ctgcacctta gacgctcagg tctacgcccc ggactccggc
     1681 aggataaaca ggcaaacggt ggaaacgtaa cttaaccgcg aaagagttaa gctctacccc
     1741 gtctcgcagt acgataactg gcacctgata aaatgagcga acaaggcagc ggtaagcggt
     1801 gaagtgtgat tggtaaggtc tgacacttca cccacacaat caagccattt gcacggcaag
     1861 agaaacaaga aacgagctag acgaaggtcg ctctgctccc cgtggtttta tatacccacc
     1921 acgggggcat taggcaaata ttaaccaaaa aaggtgatac gcccctacgt ataaggggcg
     1981 tatctatatg aaaaataccc cagcagccac aatgatctga ccagagtggc actaagtatc
     2041 agttgtctag atcacacaca gctggttata gtcatctgaa acacaatgcc actggggttc
     2101 ataatgaaaa aagcactctt agttttattt acagcgatgt ctgttcttgt ctccatgcct
     2161 tcatttgcag gtaattgtca gcatgacaat gacacagctg ctgatggttc tcgctgcggt
     2221 ggtcgctctg cagactcccg gcctggtggc ggtggtgcaa ggtaagtagt ccatttacca
     2281 taatgtctca ttgcccgcac cactccggac aggttcgcca caaacggggc ggcagtcagg
     2341 taaaaccggc aacaaaccac ccgatacacc tccccgttcg tgacagataa attttacccg
     2401 gctgcatggt catgcagcca gatgcatacg gtctgatttc aggccagaat ccggatgaac
     2461 gccttaaaaa caggaaaatg cttctccagc cagtcccaga tctccagcgc catgtacagg
     2521 cagttcagca ccgtctcaat cacgttaaac agtctcataa tccctccgtt gtataggctg
     2581 tgatgttttc atcacgaccg tatttagcgg gaaatttatg agccccttct tcgtgcgggt
     2641 tgtcactgga tgcgctcagt cagcccagcg tcggaccgtt atagctccgc tgacgggaac
     2701 gggtttccgc cagctctttt tcgtgctgtc gctgctcgta tgcccgttcg gcggccaccg
     2761 ccatttcatg ccgctgcact actggctcaa acgctgtaac tgcctgctca agttcgccac
     2821 ctgctgactc aagcgcgtca cgcgctcgtt cagcgccgcg ttctcccgtt gcataagacc
     2881 agacatcttc tgccattccg cgaaggcgct ctcccactcg tccagccttt gcgagtagtc
     2941 ctgatgtagc tgctctaatg cggtcagcaa ctgtttttcc agttccgtca tgggctattt
     3001 cctctccagt accatccagt tcccttcctc cccgtaagct cccgctttcg ggttcacttt
     3061 gacgcaggac agtttcttct ccccgcagag cgacagctgc acgccgtggt tcttctccga
     3121 cagcatggcc tgcgtccgct cctgcgcccg gatagtctgg taattcccgg tgatctgctg
     3181 cccctgccac cacagaatgc tcccgctcgt ggcgatcagc agtaccgaga ccagggtgat
     3241 ggtcagccac gtccggccga ccatacgcag cacgctcagg cggttcgact gcatcgccgc
     3301 ggtcataccc tgctcgtggg cgctgatggc gtcgctgatt ctcttcgcgc ttgcgctcag
//
