LOCUS       NZ_CP138752             4995 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 071533A plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP138752
VERSION     NZ_CP138752.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040193
            Assembly: GCF_033870135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:40:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,518
            Genes (coding)                    :: 5,392
            CDSs (with protein)               :: 5,392
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 43 of 126
            Pseudo Genes (incomplete)         :: 81 of 126
            Pseudo Genes (internal stop)      :: 17 of 126
            Pseudo Genes (multiple problems)  :: 14 of 126
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138752.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="071533A"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-07"
                     /collected_by="He Fang"
     gene            complement(<1..690)
                     /locus_tag="R8552_RS28125"
                     /old_locus_tag="R8552_28120"
                     /pseudo=""
     CDS             complement(<1..690)
                     /locus_tag="R8552_RS28125"
                     /old_locus_tag="R8552_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     gene            complement(725..1600)
                     /locus_tag="R8552_RS28130"
                     /old_locus_tag="R8552_28125"
     CDS             complement(725..1600)
                     /locus_tag="R8552_RS28130"
                     /old_locus_tag="R8552_28125"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-27"
                     /protein_id="WP_032277257.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSSG
                     GRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKPAD
                     LVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGDETF
                     RLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNTTGAA
                     SIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESRRDVLA
                     SAARIIAEGL"
                     /name="extended-spectrum class A beta-lactamase CTX-M-27"
     gene            complement(1850..3112)
                     /locus_tag="R8552_RS28135"
                     /old_locus_tag="R8552_28130"
     CDS             complement(1850..3112)
                     /locus_tag="R8552_RS28135"
                     /old_locus_tag="R8552_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /protein_id="WP_000608644.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDST
                     NKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFKTT
                     QMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQF
                     AFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIET
                     IESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRV
                     LKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGH
                     LLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTAR
                     YVIMKLSENYPYKGVYEKCLV"
                     /name="IS1380-like element ISEcp1 family transposase"
     gene            <3298..4344
                     /locus_tag="R8552_RS28140"
                     /old_locus_tag="R8552_28135"
                     /pseudo=""
     CDS             <3298..4344
                     /locus_tag="R8552_RS28140"
                     /old_locus_tag="R8552_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA];
                     GO:0007165 - signal transduction [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /name="HAMP domain-containing methyl-accepting chemotaxis
                     protein"
     gene            <4534..>4995
                     /locus_tag="R8552_RS28145"
                     /old_locus_tag="R8552_28140"
                     /pseudo=""
     CDS             <4534..>4995
                     /locus_tag="R8552_RS28145"
                     /old_locus_tag="R8552_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /name="IS5-like element IS903B family transposase"
     ARG             complement(729..1600)
                     /name="blaCTX-M-27"
                     /gene="blaCTX-M-27"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-27"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 agggataagc gcgctgattt tcttacgccg cagttcatcg tgacagagcc gggtgtcgta
       61 agcgccgtct gccgatgctg ccctgatttt tctgtgagtc tgccggataa gacccgggaa
      121 ggcttctgag tccgtcacat tgttcagcga caggtctgca cagatgattt catgtgtgtt
      181 gctgtcaacg gcgagatgca gcttacgcca tatacgacgg cgttctttgc cgtgtttttt
      241 gactttccat tcgccttcac caaagacctt cagcccggtg gaatcaatca ccagatgcgc
      301 gatttcacct cgggtgaacg ttttgaaact gacattaacc gactttgccc gcttactgac
      361 actggtgtaa tccgggcagc gcaacggaac attcatcagt gtaaaaatgg aatcaataaa
      421 accctgtgca gcccgcaggg tcagcctgaa cacgcgttta atgaccagaa cggtggtgat
      481 ggcgagatca gaatagcgct gaggtcttcc ccgtgatgaa ggcgttgccg actcatacca
      541 ggcctgaata gcttcatcat ccagccagaa agttatggag ccacggttga tgagggcttt
      601 attgtaggtg ggccagttgg tgattttgaa cttttgcttt gccacggaac ggtctgcgtt
      661 gtcgggaaga tacgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaaa
      721 ccagttacag cccttcggcg atgattctcg ccgctgaagc cagcacatcg cggcggctct
      781 ctgcgttctg ttgcggctgg gtaaaatagg tcaccagaac cagcggcgca cgaccctgcg
      841 gccagatcac cgcaatatca ttggtggtgc cgtagccgcc gctgccggtc ttatcaccca
      901 cagtccacga cgtcggtaag ccggcccgaa tgctggctgc gccggtcgta ttgcctttga
      961 gccacgtcac caactgcgcc cgctgggttt cgcccagcgc atgacccagc gtaagctgac
     1021 gcaacgtctg cgccatcgcc cgcggcgtgg tggtgtctct cgggtcgccg ggaatggcgg
     1081 tattcagcgt aggttcagtg cgatccagac gaaacgtctc atcgccgatc gcgcgggcaa
     1141 aagccgtcac gcctcccggg ccaccgagct gggcaatcaa tttgttcatg gcggtattgt
     1201 cgctgtactg caacgcggcc gcgctcagtt ctgccagcgt cattgtgccg ttgacgtgtt
     1261 tttcggcaat cggattgtag ttaaccagat cggcaggctt gatctcgaca ggctgattaa
     1321 gcagctgctt ttgcgtttca ctctgcttaa gcaccgccgc ggccgccata actttactgg
     1381 tactgcacat tggaaagcgt tcatcaccgc gataaagcac ctgcgtatta tctgcggtat
     1441 cgatgagcgc gacgcccagc cgccctccgc tgcttttctc cagcgccgcc agcttttgct
     1501 gcaccgcact cgtctgcgca taaagcggcg cgctgcccag cagcagcgga atgcacgccg
     1561 ccgccgcgaa catcatccgt tgcactctct ttgtcaccat ctcaaactcc caatacggtc
     1621 aatccgtgtt acatcagtat tccctaaatt ccacgtgtgt tttttattag cttcaaaaat
     1681 cactatttca cgaagaattt agactgcttc tcacacattg taacattatt tacaaccacc
     1741 tttcaatcat ttttgataaa tcattgattt catctttgct gcaatgatac ttaataaact
     1801 ctgcaagtta tccacagagc aacactcaat tttattgatg atattcttat tataccagac
     1861 atttttcata cactcccttg tacggatagt tttccgacaa cttcatgatt acatatcttg
     1921 cggttttgat tatttttgct gcaagaaata catacttcaa acgaaaggtc tttatttgct
     1981 gtctgtattc tgaagagtcc aaggaatcaa acttgaacaa caaaaatagg ttatatgaaa
     2041 gcatcatcat ttgaaacacg gcttcattcg cccaaaatga ctttagcaag agatgaccca
     2101 ccgccatgtc gtatttggct tctttgatat agttttcagc attaccacgc ttttcatagt
     2161 atataactac tttttcagaa agcaaggtag tatttgttac aaagaaaaag tagtcgtatt
     2221 cggaaccttc taaaagtgat aattgtgctc tttctttttc tggtttcagt acgcgagata
     2281 cgacaaatct tctgtctttt tcccatttaa ctaattttgt atacagttct gtagtttctc
     2341 taccttcttc tcctttaacg aatacaattg atgaattcgt tgcttgtgag gtgagtgtag
     2401 aataactttt ggctttaatt aaatatttgc atccaagaga ttctatcgtt tcgataattt
     2461 tttcatcaaa gtagccacta tccattcgaa ataaaatttc taaatcgtct gatttgatgt
     2521 tagcaacaat ttctttgatc atttccgcag caccgtttgc agtgtaagta ttgccacttc
     2581 ttacaaatcc ggtaacatat gcttttaatt cgtcgcaaaa tgcaaattgg atattgtagc
     2641 atcggtttcc cagtttctta ggattatatc cttttgacgc accttcttga tgaccttcta
     2701 cgttaattac actactatca atatcaatcg taatggatgt caatttactt ttagtgagca
     2761 gttttttaaa gactttaaaa ttaatgtctc taaacatttg ggttgtcttg aagttgaagt
     2821 ttcctagaaa ccgtgacact gtttcaggtt cttttacgga aatatcaaac tcgttgacga
     2881 ggggatcatt ttgaagtagc tttagacgtt ctaacttatc aatgccaatg aagtgaccgc
     2941 agagcatggt ctttatatga ttcatcttga ttttatttgt tgagtcatta tcaaatacga
     3001 ggtcattttc aataaaatca aaaatcccat tgctttttgc attctcaagg agcagaaaaa
     3061 gacctgcatt tgatgttaga ttcttagctt tgaaatcaat tttattaatc ataattagaa
     3121 ccccttttta ctacttttct tactattatt ttaccatata tcgagtcata aaagctgata
     3181 atttaacata tttttgagca cttttctttc acccaatggg tgaaagctga atttcgaagg
     3241 aatgcatatt tatcaaggct ttgattatgc tttttgaagt actgacgtag aatctaggtg
     3301 ttagccactt taatgtcagg aagcttaagc tctgttagtg ttttggctat tctctctcct
     3361 cttggaagac tggttgaaag agccaggaat atttccaata acccattaag tcaatccctc
     3421 tacactgggc gcaccgatga gtttggccaa atagagtttg ctttacgaat gatgcaagct
     3481 gaaacaggcg ccatagtagg tcgcataggt gatgcatcaa atcggcttag cgaacacacc
     3541 cgaggcctac taaaggatat tgagtcaagc aatgtactta cagttgagca gcaggcagag
     3601 acagatcaaa tagcaacggc agtaaaccaa atggtggcaa gcattcaaga ggttgcgagc
     3661 aatgcacagc atgctgcaga tgcggccgga agagcagaca ctgagacggc atctggccag
     3721 cgtctggtag cccacacaag ccagtcaatc actgcccttg aaggtgaaat taggcaagcc
     3781 actcaggtta ttcatgagct tgaaggtcaa agtaacgaga tatcaaaagt tcttgacgtt
     3841 atacgaggga tcgccgagca aacgaatttg ttggcactca acgcagcaat tgaggccgcg
     3901 cgtgctggtg agcaggggcg tggttttgct gttgtcgccg atgaggttcg cagtcttgct
     3961 gctcgcacac agcaatcgac aacggatatt caaagcatga tcagcgctct acaagagcga
     4021 gcgcaatccg ctgttacagt catggagcaa agtagtcggc aagcgcacac gagtgtagct
     4081 cacgcagagg aagcagctac agctcttgat ggaattggcc aacgcgttaa cgaaattacc
     4141 gacatgaacg cgcaaatagc gactgcggtc gagcagcagg gagcagtaag tgaagacata
     4201 aaccgcagta ttatcaatat acgcgatgct gctgatacca atgtacagac cgggcagaat
     4261 aatttgcaaa gtgcgaaatc tgtcgctcag ttaactagcg ctctgagcga actggcaaaa
     4321 cagttttggg aaaaacgagg ataacgcttt tcagtatctc gacagggata acttacttta
     4381 ccatcggtta tcccttttag ccgcacaaat tttagccatg gcttttcagg aagtcagggc
     4441 tatcagaatg gccttagaaa gcctagtcaa agagctgtca cgagaacacc gttagcttag
     4501 cgtacgattt tttccgaatt ctgcggttcc cccattaaac gcgtattcag gctgaccctg
     4561 cgcgctgcgc agggctttat tgattccatt ttttctctga tgaacgttcc gctacgctgc
     4621 ccggattaca gctgtgtcag caggcgggca aagtcggtta atatcagttt caaaacgccc
     4681 acccggggtg aaatcgcaca cctggtaatt gattccaccg ggctgaaggt cttcggtgaa
     4741 ggcgagtgga aagtcaaaaa gcatggccag gaacgccgtc gtatatggcg taagctgcat
     4801 ctggcagttg acagtaaaac acatgaaatc atctgcgctg acctgtcgct gaacaacgtt
     4861 acggactcag aggccttccc cgggttaatc cggcaaaccc accggaaaat caggtcagcc
     4921 gccgccgatg gagcttacga tacccggctc tgtcacgatg aactgcggcg taagaaaatc
     4981 agcgcgctta tcccg
//
