LOCUS       NZ_CP138750             5627 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 071533A plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP138750
VERSION     NZ_CP138750.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040193
            Assembly: GCF_033870135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:40:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,518
            Genes (coding)                    :: 5,392
            CDSs (with protein)               :: 5,392
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 43 of 126
            Pseudo Genes (incomplete)         :: 81 of 126
            Pseudo Genes (internal stop)      :: 17 of 126
            Pseudo Genes (multiple problems)  :: 14 of 126
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138750.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="071533A"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-07"
                     /collected_by="He Fang"
     gene            complement(175..660)
                     /locus_tag="R8552_RS28070"
                     /old_locus_tag="R8552_28065"
     CDS             complement(175..660)
                     /locus_tag="R8552_RS28070"
                     /old_locus_tag="R8552_28065"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142273022.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMIRTSVSTAMAWVFLCFALLIGILGGIIWFQGTIITSRLSEMD
                     SYSQQLASLKAKSGAGVTIYSDDTRRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<707..1129)
                     /locus_tag="R8552_RS28075"
                     /old_locus_tag="R8552_28070"
                     /pseudo=""
     CDS             complement(<707..1129)
                     /locus_tag="R8552_RS28075"
                     /old_locus_tag="R8552_28070"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1338..1664)
                     /locus_tag="R8552_RS28080"
                     /old_locus_tag="R8552_28075"
     CDS             complement(1338..1664)
                     /locus_tag="R8552_RS28080"
                     /old_locus_tag="R8552_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_042927524.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRR
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQTDTLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1892..3652)
                     /locus_tag="R8552_RS28085"
                     /old_locus_tag="R8552_28080"
     CDS             complement(1892..3652)
                     /locus_tag="R8552_RS28085"
                     /old_locus_tag="R8552_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="WP_009652795.1"
                     /translation="MARDDFNSDTRTVGLARLANSLEETYGSFINMENTNSKGEARREV
                     LITRCIAAHAVRMYGDELDPGVAAASVCDGSDDGGIDAVYVNPSSKKLILVQSKYIKDG
                     NGSVTIKDFGRFKDACAKVIANDLNSFNDRFLEHKESVTTAITSSSYKCICVFIYSGTQ
                     GLSSEVIGEIDFWEKSQNKALLFRDLESRDEYTILFETPNVTDISTSLSNQSTGAIDIS
                     NVILESYGEITEPYGAIYGTIAARVIHDWWKKYKYALFEKNIRSVLGDSTSVNAGIMST
                     IENSPENFWYYNNGITATYEEVDESIANAGASRKIGIFGFKKLNIINGAQTVSSIGAIF
                     DSIPEEKKDKARVMIRFINAKDPVFLGDVTKYNNTQNRVTGRDFTTQRPEQQKIQREIN
                     FVGGYTYKLLRQEDSGIAASNVIDIDDSLNALICESKKPQLIATLKSNRGRFFDTTEGN
                     LYEQVFNPRNPPSGLSVINAVNLYRVCFDVLKDTITKASNSSEDEYSKIPSLLTHGNYV
                     FISILMGKIQPINTVNGIKTYDKNSVSFDTLELAKSIFEYIQSNYPNSYLARFFQNKEK
                     VEQIITHFSS"
                     /name="AIPR family protein"
     gene            complement(3639..4541)
                     /locus_tag="R8552_RS28090"
                     /old_locus_tag="R8552_28085"
     CDS             complement(3639..4541)
                     /locus_tag="R8552_RS28090"
                     /old_locus_tag="R8552_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_009652796.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALETSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERASAYNLQFAAPLD
                     EKEVNGIAKSIAKWTEKNFTASSFKEYVINSHRSDIQSKRGRLGGLKSKGGGRPTINSP
                     WEGMGISKSTYYRNIHGKR"
                     /name="replication initiation protein"
     gene            complement(4734..5018)
                     /gene="nadS"
                     /locus_tag="R8552_RS28095"
                     /old_locus_tag="R8552_28090"
     CDS             complement(4734..5018)
                     /gene="nadS"
                     /locus_tag="R8552_RS28095"
                     /old_locus_tag="R8552_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            complement(5020..5352)
                     /locus_tag="R8552_RS28100"
                     /old_locus_tag="R8552_28095"
     CDS             complement(5020..5352)
                     /locus_tag="R8552_RS28100"
                     /old_locus_tag="R8552_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_029498755.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKVRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(3..1348)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.096"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctcgaacatg tgctgcaaac tgctgtagct gtcctgccac gcctgctgct gttgctcgta
       61 gcttgtctgt aagctctcta aggcgctcag aagctgtttt tccatctcgg tcatggttta
      121 ggtcgtcctc ggtgttaaaa acgtgctgtg gcgtttcagg gcgtgctgac ggggttattt
      181 cgccgtgtat ttcaccacgg tgttgtcggt cgtcgacgtg taggtttcca cgccgtgcgc
      241 ctgcttcggc agcaccacca ggtaggtgtt ccggcgcgtg tcgtcgctgt aaatcgtcac
      301 gcccgcgccg cttttcgctt tcaggctcgc cagttgctgg ctgtagctgt ccatctcgct
      361 caggcgcgag gtaatgatcg tgccctgaaa ccagattatc ccgcccagga tgccgatcag
      421 cagggcgaag cagaggaata cccacgccat cgccgttgag acggaggttc tgatcatgcc
      481 cgtcagcctg ccgttctgct cgcggatagc gttgctgatg ctctgcgagc ttgattgcag
      541 ttccgcgctg atagacgtct ccagcctcgc gaactcgcgc ttcacgctct gctcggtatc
      601 ctgcgcctgc tgcttcgatt tctcctcgaa gcccttcgcc aaatttaaaa tctcgctcat
      661 atatcattcc tttcagccgg atatttcggc cgccgtccgg atcggcaatg ctgatgctgc
      721 ttttcgtttc ccgcgccacg atcaggccgt atctttccag ctgcgtcacc acgtcctgcc
      781 ggctgcggat cacgccctgc tgcatcaggt tcatcaggcc ggcggtaatt ttctctgccg
      841 cctgctgttt gtcgcggggc agatcgctgg cctgcgtcag cgggcggcgg taagtcgggt
      901 cgttcgggtc gcgcagcccc agccggtcgt tggtcagggt ctgccaggcg ttgacgcggg
      961 ggcggtcggc gcggtcaaaa tagggttgca ggcgtttgcc actctgcaat tcgatgttcg
     1021 ggatgacaaa attcagctca agccgtccct tgtcgcggtg ctctacccac aggcaagcgt
     1081 actggtcgcg gtcgagaccg gtcagcagcg tgtgctccca ttcgtccatc aggcgcgatt
     1141 tctccgcgtc tgcaatgtcc ggctcctgga atgacagcac gccggaggtg taggcgcggg
     1201 caaagtcgca gccgtcaatc agctcgcgca cgtattcagg attgccgcgc agcacgcggg
     1261 cctgttcacg ctgccggtct tttcccagca ggtaatccac cggaccgctt cccgaacccg
     1321 caccacgcga atgaatttta acgatcacgg ttctgctcca gcaggtcgcc aagctgacgg
     1381 tcaaggctgg tcagtacgac aagcagcgaa gcacgttcag agggcgtcag ggtgtcagtc
     1441 tggttcagac ggcgggcgat ctggttcagg ttgttaccga ttccgctgac ctgtcgcagc
     1501 aattcgggcg ccacatcggg cacacggcgc ctgcgtgaag tgtgttcacc cagaccaagc
     1561 tgacgcagcc actccgcaag gtgagtccgg tcgcagcgat caagcagtcg ctgatgctcg
     1621 gtttcggtga gccggatttt gatctctttc gttcgctttt ccataggctc cgctgagaga
     1681 aaattcgcac cactgtgaga atttttcgca gtggatgccg ggggtttcgg ggggcggcga
     1741 gccccctgaa ccagtcacag tggcacctcg ttagagggga cgctgtgtgt actggcttag
     1801 tgtaaggtgc agtatgtcag attatgtggt ggctgcaaaa aaaaagccct ctgctagaga
     1861 gggctgttat ctgatatagg cttgtttagt ctcacgatga aaagtgagtg atgatctgct
     1921 ctactttttc tttgttctgg aaaaatctag ccagatagct gttaggataa ttagactgga
     1981 tgtattcaaa tatgctttta gctagttcaa gagtatcaaa agagacgcta ttcttgtcat
     2041 aggtttttat gccattaact gtatttatgg gttgtatttt ccccattaaa atactaataa
     2101 atacatagtt accatgcgtc agcagtgatg gaattttact atattcatct tctgatgagt
     2161 tagatgcttt agtaattgta tcttttaaaa catcgaaaca tactctgtat aaattaacag
     2221 cattgataac agaaagaccg gatggcggat ttcttgggtt aaaaacttgt tcgtaaagat
     2281 tgccctctgt tgtatcaaaa aaacgacctc tattagactt caaagttgca ataagttgag
     2341 gtttctttga ttcgcaaatt agtgcattta atgagtcatc tatatcgata acattagaag
     2401 cagctatccc tgaatcttcc tgtcgtaaga gcttatatgt ataaccccca acaaaattta
     2461 tttctctctg tattttttgc tgttcaggtc tttgtgttgt gaaatctctg ccagtaactc
     2521 tattctgagt attgttgtac ttagttacgt cacctaaaaa aacaggatct ttcgcattta
     2581 taaacctaat catcactcga gctttgtctt ttttctcttc gggtatgcta tcaaatatag
     2641 ccccaataga gcttacagtt tgcgccccat ttattatgtt taacttttta aatccgaata
     2701 ttccaatttt ccgagatgct ccagcattag ctatgctctc atctacctct tcatatgttg
     2761 cagtaatccc gttgttatag taccaaaagt tctctgggct attttcgatg gtagacatga
     2821 tccctgcatt tactgatgtc gaatcaccca agacacttct tatatttttc tcgaaaagcg
     2881 catacttgta ttttttccac caatcatgta tcacccttgc agcaatcgtt ccgtagattg
     2941 cgccgtaagg ttcagtgatc tcaccatacg actctaatat tacattactt atatctatag
     3001 ctccggtaga ttgattgctc aagctagtag atatgtcagt aacattaggc gtttcaaata
     3061 gtattgtata ctcatcccta gattctaaat ctctaaacaa cagcgctttg ttttggcttt
     3121 tctcccaaaa atcaatctct ccaatcactt ctgagcttaa cccttgggtt cctgaataaa
     3181 taaaaacgca tatgcattta tagctactac tagtgatagc cgtagtcact gattctttgt
     3241 gctctaaaaa tctatcatta aatgaattga gatcgttagc tataactttt gcacatgcgt
     3301 ctttaaaccg tccaaaatct tttattgtta cagaaccatt cccatctttt atatattttg
     3361 actgtaccaa aataagtttt ttagaacttg ggtttacata aacagcatca attcccccat
     3421 catcgctacc atcgcaaact gatgcagcag caactccagg gtcgagttca tcaccataca
     3481 tccttactgc atgcgcagca atgcatcttg tgatcagaac ttctcttcta gcctcacctt
     3541 tagagttagt gttttccata ttaataaaag aaccataggt ttcttctagg ctattagcta
     3601 accttgcaag cccaacggtt ctagtgtcgc tattaaaatc atctcttgcc atgaatattc
     3661 ctgtaatatg tcgatttact aatccccata ccttcccacg gggagtttat tgttggcctg
     3721 ccgccacctt tgctttttaa tccgcctaaa cggcctcttt tagattgaat atcgcttcta
     3781 tgagagttaa ttacgtactc tttaaatgaa gatgctgtaa agtttttttc agtccatttt
     3841 gcaatgcttt tagctatccc attcacctct ttttcatcca gaggtgcagc aaattgtaga
     3901 ttataggcac tggcgcgttc gtagcaggct tgcagccatt gctcatactc aggccagccc
     3961 tgacggatgg cgcggtatgc ccacttgcgg gttttgtcga acaacgtgca gttacggcct
     4021 aatccgtagt cggcaacgat ttctttgtcg ttcgcagcat tcaggtcgag aaagtcagcc
     4081 agccagtcca gcgtgtagag ttccggctgc catacggcaa ttttccagtg gccgtggttc
     4141 ggatttttgc agattagccc cgaataccct gcgtcagcct ctaatttttt acgtagcgca
     4201 ttctccacgg cggcggcgta gcgcaatggc ttcatttttc cgtccggcgc ggtgcgtatc
     4261 gaggtttcca gcgcgtacag caggtgtgcg tgtccgttgt cggggttggt gatggtcagc
     4321 gtgggcgcgg gggcgttgcg gtcgctccag tcgatggcgg caccggcgcg atcgacgtcg
     4381 aatcccagcc agaacatggc gtgaggctga ttgaactgga tatatttcgc cagaatggcg
     4441 cgttccttac cggcaatgcg tacaccgaag tgcagatcgt ccgaaaagta cggcttgtgc
     4501 ggtaagcgtt cgttgaagag ttcgagtgcg gcgttatgca atgcgccctg cgcttatggc
     4561 gggctgttgt ctggcacggt tcatgtgcta atatccttca taggtttcga cctggccctg
     4621 gtagtcatgc tcatccgcca agatagctca tgattgccgg ggctttgttt tttctgttgc
     4681 cgttaaatta cacgcgaacg gcctcgcctg tcgaatcctt ccttcacaac ggcttagtgc
     4741 gccgccagct cctggaagaa tttcggattc gcctggatgg ttgccagcac cttcgcagcc
     4801 agtccggttg gattgcggcg tccggtttcc cagctcttga tggtgtcgag actggttccc
     4861 agcactttcg ccatctcgct ctgtgaaacg tttagctgtg ccctgatggc cttcacgtct
     4921 gccagctcgt aacgggtgac gcgggcaggc tctctgacgc cgtttttgat ttcaacggct
     4981 tcttccagcg atgctttcag ctcgtcaaaa aaactcatgt cacacctcgt ctttcagttg
     5041 ttgggtcagc gtcttcagcg cggctttctc cgcgtccgtc aggctgtctt tggtgctttt
     5101 cgggtaggcc atcaccagat aaatcacctc cgcggtggcc agaaaataaa tcaccctcgc
     5161 actgccgctt tttccctggt tgcccgtcgc catcctgact tttcgcagcc cgccagtttt
     5221 ctggatgaga tcgcctttgt ccggcgactc aatcagcgtc ttttgcagtt ccctgagctc
     5281 ttcatccgtg gcgatctgtt ttatctgacg ggtaaacatc ggggtctcga taaactcaat
     5341 cgcgtgactc acggctgtct cctgtgctta atggcgtaca tagtacactt aattctgatt
     5401 tcggaaaagc ggtcacatcg aggggccata gtagcgggtc ggctttttct gtggctccgg
     5461 ctgctgctca cgcagcttct cctcacgggc gcggtcatgc tgcgcgatca gcgcatcaag
     5521 cggctgacgt tcctgctcaa gctctcgacc tgcgcgcgca agctgtccac ctgactgctc
     5581 aagtgctgac acacccggtc gctgtgcgtc agcgcctgtg aggtagc
//
