LOCUS       NZ_CP138743             4574 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 0804007 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP138743
VERSION     NZ_CP138743.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040192
            Assembly: GCF_033863175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:37:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,759
            Genes (coding)                    :: 5,563
            CDSs (with protein)               :: 5,563
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 70 of 196
            Pseudo Genes (incomplete)         :: 124 of 196
            Pseudo Genes (internal stop)      :: 34 of 196
            Pseudo Genes (multiple problems)  :: 26 of 196
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138743.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="0804007"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-08"
                     /collected_by="He Fang"
     gene            join(4510..4574,1..49)
                     /locus_tag="R8538_RS29365"
                     /old_locus_tag="R8538_29365"
     CDS             join(4510..4574,1..49)
                     /locus_tag="R8538_RS29365"
                     /old_locus_tag="R8538_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            542..652
                     /locus_tag="R8538_RS29370"
                     /old_locus_tag="R8538_29370"
     CDS             542..652
                     /locus_tag="R8538_RS29370"
                     /old_locus_tag="R8538_29370"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_165474164.1"
                     /translation="MDWILLAIAIALLVVAVYSGVSYFREKKTISRAFRR"
                     /name="small membrane protein"
     gene            1707..1928
                     /locus_tag="R8538_RS29375"
                     /old_locus_tag="R8538_29375"
     CDS             1707..1928
                     /locus_tag="R8538_RS29375"
                     /old_locus_tag="R8538_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947202.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            1921..2808
                     /locus_tag="R8538_RS29380"
                     /old_locus_tag="R8538_29380"
     CDS             1921..2808
                     /locus_tag="R8538_RS29380"
                     /old_locus_tag="R8538_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_048290304.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            2805..3503
                     /locus_tag="R8538_RS29385"
                     /old_locus_tag="R8538_29385"
     CDS             2805..3503
                     /locus_tag="R8538_RS29385"
                     /old_locus_tag="R8538_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290303.1"
                     /translation="MTRTFILLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYIGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTELARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(3510..3908)
                     /locus_tag="R8538_RS29390"
                     /old_locus_tag="R8538_29390"
     CDS             complement(3510..3908)
                     /locus_tag="R8538_RS29390"
                     /old_locus_tag="R8538_29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_187981993.1"
                     /translation="MATVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLETSE
                     LLRVFGAFASVSVEQEEEQVSGQRDTVTSDHNEQVIHTLKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREDQRQNTTRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3908..4099)
                     /locus_tag="R8538_RS29395"
                     /old_locus_tag="R8538_29395"
     CDS             complement(3908..4099)
                     /locus_tag="R8538_RS29395"
                     /old_locus_tag="R8538_29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          4148..4574
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.046"
                     /coverage="92.0"
                     /evalue="1e-154"
ORIGIN
        1 cccggaaaga catgcaaaag cgccactggc agcagccact ggtaactgat ttagaggaga
       61 tagtcttgaa cttatgcgcc ggttaaggct aaactaaacg aacagttttg gtgactgcgc
      121 tcctctaact agccagttac ctcggttcaa agagttgctg ctctgagaac cttcgagaaa
      181 accgccttgc tgggcggttt tttcgtttac agagcaagag attacgacga ggctgaatga
      241 atctcaagat ttcatttagt ctgccccccg atcattcaaa gttttggcgt agttctgacc
      301 cgccccccgt gatttgttat atacccacca cgggggcatt agtttttttt attttttttt
      361 ttatgatatg tacaaaacca gtggttaaga ataatcttag cagccttaac aaaacacata
      421 caaaaacagc ggttatgttt catctcacca caataagtaa cgacagtatc atgctaaaaa
      481 aagatgatat tatctttatg aaatcataac aggctgtttt tattaaatta atcaggaatt
      541 tatggactgg attttacttg ctatcgccat tgctctactt gttgtagctg tctactcagg
      601 agtctcatac ttcagagaaa aaaaaacaat cagtcgtgca tttaggcgat agcgccctat
      661 ttaccataat cccttcttac gcgcaccgcg aaaacggcct cagggcgttt ctggtggtta
      721 acgggcaaaa ggtggtggca agtacaccga aaaccgccag aaacgcgcct gatttatttt
      781 acgcatatgc atggctatgc acaccaccgc atagccggtg atttcggccg agaaaagggc
      841 cccggacgtt aaaagcataa aagaacgggc catccaggcc cgtcaaatga ggacaacact
      901 gtaaaaggac cgtcgcacct ggtgcggtgc cggcgacaag aaaaagatag ctcatatcag
      961 cccctcaccg gagaaaaata tttcctggcc acctcaaccg ccacacgctg tgttcccgtt
     1021 aactcgcgca gataacgcag gaacatcctg ttttcctctg catgcgcctc ctcactgccc
     1081 tgtgcgtggt attccactat cgcgttcacc acggactcac cgacctgcat cgtctgacct
     1141 catctgcact ttcatcacac cgtttcagat cccgcctcct cgccatgatg tgaaacggtg
     1201 accgggcagg caaatttttt gtctgtccgg tcaccgttgg cggggtccgg ggcggtcagt
     1261 cccggtgttt tttattccgc caaatcagca cctgcgacac ccgctatgta cgacgcataa
     1321 atgcgcctgt acatagcggg tgtcgctatt gagcggagtc aggcgcaaca tttaaaaaac
     1381 aacattcaca cgcacgaaaa acctggctag tatttagtca aaccatgagg aggcgcactc
     1441 tgacgcgcac ctgcctaccg gcagcaggta aaacagccgg gtccctccgg ggatactgta
     1501 ccgggcagcc caatccgaga atcctgagaa aacagctaaa atctgcgaga ccggcaatca
     1561 ggtcaggcca ttccctggct taagggacgt tatcagccgc gagcagtccg ggctgaacca
     1621 cgagaggttc gaacgagggc gatcggcgca tgccgcagtg accgttaacg taacggccac
     1681 tgatgttctt acacaccgga gcacagatgc aatttgatac cgctgccgaa ctggccgccc
     1741 tgcaggcgca gacccgccgt atccgccagg tccgctaccg tccctcccgc cttgaccgct
     1801 ataccggcga actgctgagc ctgtaccagg ccggggccag tgcagcggag ctgcaacgct
     1861 ggctccgggc ccggcgaatc aaggtggtgt tgtccacggt tacccgctgg ctggaaaaaa
     1921 atggctaaat tcgccagcgc tgcccgccag gcggctggcg tgataaaacc actccaggga
     1981 aatattctgc gctctgtcgg cacagtccgg aattacgagc agggactgac ccgcgtcgca
     2041 gagtggatga aagccaaccg gattaatcac ggcgatttac gcacactgac accggcgcag
     2101 gccatcacct acctggagca gcgcggggag caggttggcc agaaaacgct cgatatggaa
     2161 cgccaggcca tccaggccat gatgatccac gtgacgcaga ccctccctgc cggcgaacac
     2221 ctgccggtgg tgaaatcgga gcaggcacag atcctgagca gccgggccta cagcccggac
     2281 caggtagccc tgattgccgc agcacaacag cccgccaacg ctctggccac cgcgattgcc
     2341 ctggccgccg gattacgcgc ccatgaactg ctgaccctcg cgccccgcca ggagcgagca
     2401 gcggatcccc gtccggcagc ggacagtaaa tttgccggtc gggagggtgt gatttacacc
     2461 gttcagggca aaggcggcct ggtcagggaa gttctgctgc cgtcacgcct ggctgaacgt
     2521 ctcgagcatg tcaggctggc cagcccggtc cgggtgaccg atcgcggcgt gttttaccag
     2581 agtcagtacg ccattggcgg aggccagcgc tggtcaaacg ccttcagtgc cgcctccatg
     2641 cgtaccctgg gctggtcacg gggggcacac ggcgtgcgtc acagctacgc ccagcaacgg
     2701 atgcaggagc tgcaaaaact cggtctggtt cgtgatatgg ccctgcgcac cgtcagccag
     2761 gaaatgggcc attttcgtcc ggaaatcacg gagacatacc tgcgatgaca agaacattta
     2821 ttttattgtg taccggtgtt ttcgccatcg cgtacgccct gtatgagctg aatcactatg
     2881 gcgtttacta tccggacgtc agcaatcctg aggcgtttac tggctggaag gcggtcaccg
     2941 aactcggtcc cgctatcctg gcaacgggga tcgcagccac agcgttctcg ctgtggctgt
     3001 tcattttgtt tttcagaggc tcagagtccc gccggataca gcgcgagata agcggatatc
     3061 agcaggcact gagggaggca gaagaggctc acagagccag acaaaatacc cttattcaga
     3121 atcacaacct ggagacagcg gccctgcgaa gccactacat tggtgctgag gatgtactgc
     3181 gggaagcgtt tgcgcaaaaa gaaagcctct tgcttcaggc ggtgcagaat gcccggaaag
     3241 aagccagaaa ggagctggaa gaacagaggg aggcactgaa tcagcgcgaa tccatcctgc
     3301 agaaaaagga gctggcgctg gaaacaaccc tggagaaggc ccggaaatat gtggccgggg
     3361 agcggcataa caccgagctg gcccgtctgg aagcagagaa agcagaggaa agacggagaa
     3421 atgcctgcgc gacggcggaa cggcgcagac ggaaactgga aaaactccag gccactggcg
     3481 gaacagcagg gcaaaaacac tgaggcattt caggactgcc agaaacgcca ccagggccgg
     3541 gtcgtgttct gccgctgatc ttcccggttc tccagcaaca acatcgcctg tttcagactg
     3601 tcgatatgtg aatcttttga ggcaagaagt gtgttcaggt gttcaatttc ctgtttcaga
     3661 gtatgaatga cctgttcatt atggtcagaa gtgactgtgt cacgctgtcc actgacctgt
     3721 tcttcctcct gttcaacgga tacggacgca aaggcaccaa acacccgcaa aagctctgaa
     3781 gtctcgagca ttttcgcatt ccgttcaccc cgacaggtgg acagctcacc ggctttaatt
     3841 aacctgtgca gggttgtcct gctcttaccc gtcaggcgtg cagcctcact gatcgacact
     3901 gtcgccatca gtcaccctcc tgtagtgcat ccagtctgag ggacagtgta cggaaaagat
     3961 gctccgcatc atcgtggagt ttctcacaaa ggtcagcctc cgcatcgaga tcaagctcgt
     4021 taagcttctc cagcaggagc agggactgat tctggatgaa tttagccatg ttcagggcag
     4081 ttttctgttg tttcgtcata aggggcctca gggcgtcagt gctcagtgta aaaccagtct
     4141 cttttccgct tcctcgctca ctcagtcgct acgctcctgc cgttcggctg cggcgagcgg
     4201 acgggacaca cagaagagtc atcgcgataa ccaggaagaa aagagcctgt ggaaaaggcc
     4261 acggcgttgc cgtttttcca taggctccgc ccccctgaca agcatcacag aaacggacgc
     4321 tcaaatcagc ggtggcgaaa cccgacagga cttaaagata ccaggcgttt ccccctggtg
     4381 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     4441 ggccgcgttt atctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     4501 ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgccttatc cggtaactat
     4561 cgtcttgagt ccaa
//
