LOCUS       NZ_CP138717             7263 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain SRY516 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP138717
VERSION     NZ_CP138717.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040188
            Assembly: GCF_033859755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:01:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,573
            CDSs (total)                      :: 5,451
            Genes (coding)                    :: 5,288
            CDSs (with protein)               :: 5,288
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 62 of 163
            Pseudo Genes (incomplete)         :: 103 of 163
            Pseudo Genes (internal stop)      :: 23 of 163
            Pseudo Genes (multiple problems)  :: 24 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138717.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SRY516"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-03"
                     /collected_by="He Fang"
     gene            complement(join(7086..7263,1..122))
                     /locus_tag="R8551_RS27810"
                     /old_locus_tag="R8551_27810"
     CDS             complement(join(7086..7263,1..122))
                     /locus_tag="R8551_RS27810"
                     /old_locus_tag="R8551_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_102012864.1"
                     /translation="MQYDVEWKAGAIEDVTALFEYIAENSSLWDAKNVTERLLSSTDKL
                     AGFPRLYEVDNRYGEDVRRISLMGQHILYEVDDTARKVRVLAVVGQRQNPHSIR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(112..375)
                     /locus_tag="R8551_RS27815"
                     /old_locus_tag="R8551_27815"
     CDS             complement(112..375)
                     /locus_tag="R8551_RS27815"
                     /old_locus_tag="R8551_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_102012865.1"
                     /translation="MSKSTHYENANFLRELAESLPRILPTGSADKAALLQRLANEELEQ
                     AEYEERVRAKVEAARADTRPGMSTDQLRQQLLSRYQEKHDAV"
                     /name="plasmid stabilization protein"
     gene            complement(745..1158)
                     /locus_tag="R8551_RS27820"
                     /old_locus_tag="R8551_27820"
     CDS             complement(745..1158)
                     /locus_tag="R8551_RS27820"
                     /old_locus_tag="R8551_27820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142371942.1"
                     /translation="MKIINLVSLSGALLISACSVSQNEMNSANYGSKPINNEEKVIDMI
                     KESLKDPESLRVLSITNPARGFSKYGFGSTEHGWFTTITYNAKNSYGGYVGAKSSTFVY
                     LNGDYKVAVPMSNGERVMLDKVSFSCKDNCQVK"
                     /name="hypothetical protein"
     gene            complement(1272..>1493)
                     /locus_tag="R8551_RS27825"
                     /old_locus_tag="R8551_27825"
                     /pseudo=""
     CDS             complement(1272..>1493)
                     /locus_tag="R8551_RS27825"
                     /old_locus_tag="R8551_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011145420.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /name="putative addiction module antidote protein"
     gene            2671..2892
                     /locus_tag="R8551_RS27830"
                     /old_locus_tag="R8551_27830"
     CDS             2671..2892
                     /locus_tag="R8551_RS27830"
                     /old_locus_tag="R8551_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            2885..3772
                     /locus_tag="R8551_RS27835"
                     /old_locus_tag="R8551_27835"
     CDS             2885..3772
                     /locus_tag="R8551_RS27835"
                     /old_locus_tag="R8551_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_319795796.1"
                     /translation="MAKFASAARQATGVIKTLQGGILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQALPAGEHLPVV
                     KSEQAQILSSRAYSPDQVVLIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLIPSPLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     gene            3769..4467
                     /locus_tag="R8551_RS27840"
                     /old_locus_tag="R8551_27840"
     CDS             3769..4467
                     /locus_tag="R8551_RS27840"
                     /old_locus_tag="R8551_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319795794.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQSETDRYKQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYSGAEDVLREAFAQKENLLLQAVQNAREEARQELEEQTKALNQRAS
                     GLLVQEQALKARMAEVRKYVAGERHNAMRARLEAEKAEKRRQNASATAERRRRKLEKLQ
                     ATDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(4474..4878)
                     /locus_tag="R8551_RS27845"
                     /old_locus_tag="R8551_27845"
     CDS             complement(4474..4878)
                     /locus_tag="R8551_RS27845"
                     /old_locus_tag="R8551_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_319795795.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFAPVPVEQAEEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTNRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4878..5069)
                     /locus_tag="R8551_RS27850"
                     /old_locus_tag="R8551_27850"
     CDS             complement(4878..5069)
                     /locus_tag="R8551_RS27850"
                     /old_locus_tag="R8551_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5489..5602
                     /locus_tag="R8551_RS27855"
                     /old_locus_tag="R8551_27855"
     CDS             5489..5602
                     /locus_tag="R8551_RS27855"
                     /old_locus_tag="R8551_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            5986..6324
                     /locus_tag="R8551_RS27860"
                     /old_locus_tag="R8551_27860"
     CDS             5986..6324
                     /locus_tag="R8551_RS27860"
                     /old_locus_tag="R8551_27860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142372192.1"
                     /translation="MKYLFVTVAAISLTGCISSSGVLPMGPDTYSISVHAAPVRGQAGA
                     KKAALTEANQYCMQQGKTMLVKNMDHSTFSSLPGDDLNIIFQCLSNGDQELQRPNYQSP
                     TDININVH"
                     /name="hypothetical protein"
     gene            complement(6391..6786)
                     /locus_tag="R8551_RS27865"
                     /old_locus_tag="R8551_27865"
     CDS             complement(6391..6786)
                     /locus_tag="R8551_RS27865"
                     /old_locus_tag="R8551_27865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142372193.1"
                     /translation="MNANEVKKVLEESSQEDWIVDDETGSFTYKNDLNLHIERADYDNY
                     NSFNEPWAVCHPNPNACSVQYTVKYGSSFVDRKTLVSVDGHRATLPMPASAKDLTVKSA
                     DVNFAKIVDVGDRVDEYIIRSGLKVNP"
                     /name="hypothetical protein"
     Typing          5118..5602
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.959"
                     /coverage="92.0"
                     /evalue="9.76e-175"
ORIGIN
        1 gaagacagaa gccgttcagt gacgtttttt gcgtcccaga gtgatgagtt ttccgcgata
       61 tattcaaaaa gtgccgttac atcttcgata gctccggcct tccattccac atcatactgc
      121 atcgtgtttt tcctgatacc ggctcaggag ttgttgtcgc aactggtcgg tactcattcc
      181 aggacgggta tcagcccgcg cagcttcaac tttagcgcga acccgctctt catactcggc
      241 ttgctccagt tcttcatttg caaggcgttg taataaagct gctttgtccg cgctgcctgt
      301 aggaagaatg cgcggcagac tttctgccag ctcccgcaga aagttagcgt tttcatagtg
      361 agtgctttta ctcatggctg tagctcctga tttcccgtac atattggaga tagtttagca
      421 gtgggtgtat tgcgggtcat gtgccagtgg catctttgtc tgacggcaag tgttcatgat
      481 cttcctgggg tcagtttgga gataggaaaa ttttgtacgt taagcgaata atttaaccaa
      541 ataatattcg taacctattg atttaattca gaagctatcg cttttaagtt gtcgctcttg
      601 cgaggttatc cgattaagga caccctattt ccgcacaata attctctata tccaaactgc
      661 ccccaagtag gttttgggaa gaactgtcca gaagaagtaa aaatgatagt gaataaaaaa
      721 ggccgcaatc gcggcctttt ttattcactt aacttggcaa ttatctttac atgaaaatga
      781 cactttatct aacataactc tctcaccatt agacattggc actgccactt tataatctcc
      841 atttaagtaa acgaaagtac tagacttagc accaacataa ccaccataag agttttttgc
      901 attatatgtg atggtagtga accaaccatg ctctgtagag ccaaatccat acttagaaaa
      961 acctctagca gggttagtta ttgataaaac tctcagagat tcaggatctt ttagactctc
     1021 cttaatcata tcaattactt tttcctcgtt atttattggc ttagaaccat aattagcact
     1081 attcatttca ttctgggaaa cagaacaggc agatattaac agagcaccag atagtgaaac
     1141 caaattaatt attttcatat atatccttat ttttttcaat aatatctata ggcaaatact
     1201 acattgccat ttatagatag actcaccata acaccttaac tacacaagct ctacactact
     1261 taataaaaaa cttaggctat caaagctatc tgggaaaact gtaatccggt agcagaaaca
     1321 acacttctca gggttttgag tgtcggattg ccttttggtg agagcgtccg gtaaagcgct
     1381 tctcgggata caccggcacg agcagcaatc tgagccattc caccacgcgc ctccaccaca
     1441 tgacgaagtg cagacaaaaa agcttcttgt cctccttcct catcgatatc gacaaatgtc
     1501 tggtctatgt agggtcagtt tggagatcgg aaatgattgt acgtttaggg cgttatttta
     1561 cgttcagaac ccaatgccgg ggggcggggg ttattgttct gtctgtcagc aacatccagt
     1621 cgatttacca taatcccttc ttacgcgcac cgcgaaaacg gcctcagggc gtttctggtg
     1681 gttaacgggc aaaaggtggt ggcaagtaca ccgaaaaccg ccagaaacgc gcctgattta
     1741 ttttacgcat atgcatggct attcacacca ctgcatagta ggtgatttcg gtcgagaaaa
     1801 gggccccgga cgttaaaagc ataaaagaac gggccatcca ggcccgtcaa atgaggacaa
     1861 ctctgtaaaa ggaccatcgc acctggtgcg gtgccggcga caagaaaaag atagctcata
     1921 tcagcccctc accggagaaa aatatttcct ggccatttca accgccacac gctgtgttcc
     1981 cgttaactca cgcagataac gcaggaacat cctgttttcc tctgcatgcg cctcctcact
     2041 gccctgtgcg tggtattcca ctatcgcgtt caccacggac tcaccgacct gcatcgtctg
     2101 acctcatctg cactttcatc acaccgtttc agatcccgcc acctcgccat gatgtgaaac
     2161 ggtgaccggg caggcaaatt ttttgtctgt ccggtcaccg ttggcggggt ccggggcggt
     2221 cagtcccggt gttttttatt ccgccaaatc agcacctgcg attcccgcta tgtacgacgc
     2281 atgaatgcgc ctgtacatag cggaattcgc tattgagcgg agtcagacgc aacatttaaa
     2341 aaacaacatt cacacgcacg aaaaacctgg ctagtattta gccaaaccat gagaaggcgc
     2401 actctgacgc gcacctgcct accggcagca ggcaaaacag ccgggtccct ccggggatac
     2461 tgtaccgggc agcccaatcc gagaactctg agaaaacagc ccaaatctgt cagacccaca
     2521 accgggtcaa gtcattctct gactaaagag acgttgagcc gcgagcagtc cgggcttaac
     2581 cacgttacgg tttaaacgag ggcgatcggc gcaggccgca gtgaccgtta acggccactg
     2641 atgttcttac agtcccactc cggagcacag atggaatttg ataccgccgc cgaactggcc
     2701 gccctgcagg cgcaaacccg ccgtatacgc caggtccgct accgtccctc ccgccttgac
     2761 cgctataccg gcgaactgct gagcctgtat caggccgggg ccagtgcagc ggagctgcaa
     2821 cgctggctcc gggcccggcg aatcaaagtg gtgctctcaa ccgtcacccg ctggctggaa
     2881 aaaaatggct aaattcgcca gcgctgcccg ccaggcgacg ggcgtgataa aaaccctcca
     2941 gggcggtatt ttgcgctctg tcggcacggt ccggaattac gagcagggac tgacccgcgt
     3001 cgcagagtgg atgaaagcca accggattaa tcacggcgat ttacgcacac tgacaccggc
     3061 gcaggccatc acctacctgg agcagcgcgg ggagcaggtc ggccagaaaa cgctcgatat
     3121 ggaacgccag gccatccagg ccatgatgat ccacgtgacg caggccctcc ctgccggcga
     3181 acacctgccg gtggtgaaat cggagcaggc acagatcctg agcagccggg cctacagccc
     3241 ggaccaggtg gtcctgattg ccgcagcaca acagcccgcc aacgccctgg ccaccgcgat
     3301 tgccctggcc gccggattgc gcgcccatga actgctgacc ctcgcgcccc gccaggagcg
     3361 tgcaccggat ccccgtccgg cagcggacag taaatttgcc ggtcgggagg gtgtgattta
     3421 caccgttcag ggcaaaggcg gcctggtcag ggaagtcctg ataccgtccc ccctggctga
     3481 acgtctggag catgtcaggc tggcgagccc ggtccgggtg accgatcgcg gcgtgtttta
     3541 ccagagtcag tacgccattg gcggaggaca gcgctggtcg aacgccttca gtgccgcctc
     3601 catgcgtacc ctgggctggt cacggggggc acacggcgtg cgtcacagct acgcccagca
     3661 acggatgcag gagctgcaaa aactcggtct ggttcgtgat atggctctgc gcaccgtcag
     3721 ccaggaaatg ggccattttc gtccggaaat cacggaaaca tacctgcgat gacaagaacg
     3781 tttattttac tgtgtaccgg tgttttcgcc atcgcgtacg ccctgtatga gctgaaacat
     3841 atgggcgttt actatccgga tgccagcaat ccgcagctgt tcactggctg gaaggcggtc
     3901 accgaactcg gtcccgcaat cctggctacc gggatcgcag ccacagcgtt ctcgctgtgg
     3961 ctgttcattt tgtttttcag aggctcagag tcccgccgga tacagagcga gacagacagg
     4021 tataagcagg ctctgaggga ggcagaagag gctcacagag ccagacaaaa tacccttatt
     4081 cagaatcaca acctggagac agcggccctg cgaagccact acagtggtgc tgaggatgta
     4141 ctgcgggaag cgtttgcgca aaaagaaaac ctcttgcttc aggcggtgca gaatgcccgg
     4201 gaagaagcca ggcaggagct ggaggaacag acgaaggcac tgaatcagcg cgcgtccggc
     4261 ctgctggtac aggagcaggc actgaaagcc agaatggcgg aagtcagaaa gtatgttgcc
     4321 ggagagcggc ataacgccat gcgggcccgt ctggaagcag agaaagcaga gaaaaggcga
     4381 caaaatgcca gcgcaacagc ggaacggcgc agacggaaac tggaaaaact ccaggccact
     4441 gacggaacag cagggcaaaa acactgaggc atttcaggac tgccagaaac gccaccaggc
     4501 acggggccgg ttcgtgttct gccgcttctc ttcccggttc tccagcaaca acatcgcctg
     4561 tttcaggctg tcgatatgtg aatctctgga ggcaaggagg gtgttcaggt gttcaatttc
     4621 ctgtttcaga atgtaaatga cctgttcatt acggtcagaa gtgactgtgt cacgctgtcc
     4681 actgacctgt tcttccgcct gttcaacggg tacaggcgca aaggcaccaa acacccgcaa
     4741 aagctctgaa gtatcgagca ttttcgcatt ccgttcaccc cgacaggtgg acagctcacc
     4801 ggctttaatt aacctgtgca gggttgtcct gctcttaccc gtcaggcgtg ctgcctcact
     4861 gatcgacact ttcgccatca gtcaccctcc tgtagtgcat ccagtcgtag ggacagtgta
     4921 cggaaaagat gctccgcatc atcgtggagt ttctcacaaa ggtcagcctc cgcatcgaga
     4981 tcaagctcgt taagcttctc cagcaggagc agggactgat tctggatgaa tttagccatg
     5041 ttcagcgcag ttttctgttg tttcgtcata agggccctca tcgcgtcagt gctcagtgta
     5101 aaacctgtct cttttccgct tcctcgctca ctcagtcgct acgctcctgc cgttcggctg
     5161 cggcgagcgg acggggcaca caggcagaaa agtcatcgcc accacccgga aaaggaaaag
     5221 aacagacggg agaaggccac ggcgttgccg tttttccata ggctccgccc ccctgacaag
     5281 catcacagaa acggacgctc aaatcagtgg tggcgaaacc cgacaggact ttaaagatac
     5341 caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     5401 ggtgtcattc cgctgttatg gccgcgttta tctcattcca cgcctgacac tcagttccgg
     5461 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgactgctg
     5521 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcgccact
     5581 ggcagcagcc actggtaact gatttagagg agatagtctt gaagttatgc gccagttaag
     5641 gctaaactaa acgaacagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
     5701 agagttgctg ctctgagaac cttcgagaaa accgccttgc tgggcggttt tttcgtttac
     5761 agagcaagag attacgatgc tacagaaagg atctcaagag gatcctgttc catatgcctc
     5821 cccctgaagt gaaaccagca ccactccgcg ataccagcta tcaaaataaa attattgaac
     5881 ccattgattt taataagaaa aaaacagata taatcatgat gcacaatcac ccttatcgtg
     5941 caattaactt gttgatttaa tatcaaaaat caaaaaggta taaaaatgaa atatctcttc
     6001 gttacagtgg ctgcaatttc ccttacaggg tgtatcagtt caagcggggt actgccaatg
     6061 gggcctgata cttactccat atcagttcac gctgcacctg taagagggca agcaggtgcg
     6121 aaaaaagccg cattaacaga agcaaatcaa tactgcatgc aacagggcaa aactatgttg
     6181 gtcaaaaaca tggatcactc tactttcagt tctctaccag gtgatgactt aaacataata
     6241 tttcagtgtt tatcaaatgg agatcaggaa ttacaaagac cgaattacca gagcccaaca
     6301 gatataaata ttaatgttca ttaagtaaca ttatctacac cagcaaccat aagccctaaa
     6361 attacacagt gggcttatat attatctact ttatggatta acttttagcc cagagcgaat
     6421 gatatattcg tcaacacgat ctccaacatc tacaatcttg gcaaaattaa cgtcagctga
     6481 tttaaccgtc aaatcttttg cggatgcagg cataggcaag gtagcacgat ggccatcaac
     6541 agaaaccagg gtttttctgt caacaaatga agagccatat tttactgtat attgcactga
     6601 acaggcgttc gggttcggat gacaaacagc ccaaggctca ttaaacgagt tgtaattgtc
     6661 ataatcagct ctctcaatat gcaaatttaa atcattctta tacgtaaaag aaccagtctc
     6721 atcatccaca atccaatctt cttgacttga ctcctcaaga actttcttta cttcatttgc
     6781 attcattaat aactccatca agcgtatttt tattacaaat aaataaaaac agtccccata
     6841 aatattatct agacatctat caacaggcaa gttatttacc ataacatccg ttagtggttc
     6901 ggaagcaacc ttagcacctg atggctcata cgttggaaat taacacccca ttattatgct
     6961 gcgtaccgat atactgaatt gcgaatgcca gcatttttgg atttaccata acgtcttcat
     7021 acgcacaccg agaaaacggc ctcagggcgt ttctgccggt gcatgcaacg gatgctattg
     7081 taaatttagc ggattgagtg aggattttga cgctggccga caacggcaag aactctgacc
     7141 tttcgcgctg tatcatcaac ctcgtaaaga atatgctgcc ccattaggct gattcgccgg
     7201 acatcttcac catagcgatt atccacttca tacaaccgtg gaaatcctgc caatttgtca
     7261 gtt
//
