LOCUS       NZ_CP138709             4167 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 14184 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP138709
VERSION     NZ_CP138709.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040186
            Assembly: GCF_033857255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 13:01:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,767
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,481
            CDSs (with protein)               :: 5,481
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 61 of 161
            Pseudo Genes (incomplete)         :: 100 of 161
            Pseudo Genes (internal stop)      :: 29 of 161
            Pseudo Genes (multiple problems)  :: 23 of 161
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="14184"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-05"
                     /collected_by="He Fang"
     gene            complement(265..897)
                     /locus_tag="R8555_RS28810"
                     /old_locus_tag="R8555_28810"
     CDS             complement(265..897)
                     /locus_tag="R8555_RS28810"
                     /old_locus_tag="R8555_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(897..2012)
                     /locus_tag="R8555_RS28815"
                     /old_locus_tag="R8555_28815"
     CDS             complement(897..2012)
                     /locus_tag="R8555_RS28815"
                     /old_locus_tag="R8555_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2357..2470)
                     /locus_tag="R8555_RS28820"
                     /old_locus_tag="R8555_28820"
                     /pseudo=""
     CDS             complement(2357..2470)
                     /locus_tag="R8555_RS28820"
                     /old_locus_tag="R8555_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2886..3077
                     /locus_tag="R8555_RS28825"
                     /old_locus_tag="R8555_28825"
     CDS             2886..3077
                     /locus_tag="R8555_RS28825"
                     /old_locus_tag="R8555_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3077..3478
                     /locus_tag="R8555_RS28830"
                     /old_locus_tag="R8555_28830"
     CDS             3077..3478
                     /locus_tag="R8555_RS28830"
                     /old_locus_tag="R8555_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3824..4066)
                     /locus_tag="R8555_RS28835"
                     /old_locus_tag="R8555_28835"
     CDS             complement(3824..4066)
                     /locus_tag="R8555_RS28835"
                     /old_locus_tag="R8555_28835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          complement(2358..2765)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 tcctgagtcc gttttagcgg tgcgtacaat tatgatttta tgttaaattg ttttaagtga
       61 atagagagaa tataacttaa gggagcatct tgtgctgggt cttttacatt agatctgcac
      121 aatcattgtt ttggcatctg gtgatgtctt taggacgttt gagattgctg ataactaaag
      181 tgtatgatgc tttctatacg ttatccggcg tttaataaca ccctggtgat aagccagccc
      241 gtgaagggct ggcttgattt ggatttatat ttttgaaata aaatctttta tttcatcaaa
      301 gcaattgtct gctgtgttta ttattccatt tttcatggat attcctattc ttgagtcgga
      361 ttctctattg cagctaagat agacttgtac cttagctttt ttttcgtttg ttggataagt
      421 gtactctttg tcactcgctg agatgtcatc tttattattt ttaatttttt cgaaaaattc
      481 tattatgtat tcatataacg ctcgagtgct tgcgtgttca attatcagat cttcaatggc
      541 accttgcttg tcttttcctg gcattataaa gaaagaaatt tttgtattgc cattcatagc
      601 tatctcgtta tgcgagctgg gggaggagat ttgtagttct cgcaatgtgg caattattga
      661 tctttctgtg tcagaaaatg atgtgtcagc atctaatata ataaaaatac tttctttttc
      721 atcgaaagaa tcaatgtttt tgaatgctct aagtgcttgt tttaattttg attttccatt
      781 tattttttcg acaaaaacat cggtaatgcc ttgtatttca agtaggttgc atagaaaatg
      841 aaaatcatca tcaccctcaa cgaaaatgat ctttttgcta ttatttatgt ctttcattat
      901 cttacctccc agtcgttctc aactgagtag gagaaatcat ccatattgaa ttgtacggct
      961 gttggtttta gggagttctt ttctcttcct agtctaatat aagcgatatc ttcatttttg
     1021 ctatctattt cgtttattgc tcgtatcaca tcttgactat gagttgttgc aaatatttga
     1081 ttgttgtttt gttttgcaga tgaaattaat gattttatta tgtctttaat tactgagtaa
     1141 tgtattccgt tctcgatttc atcaataaga ataattgagt tttcctgtac cagcactgag
     1201 ctgataaaag ataatgctcg actgataccc tcgccaagca ttgatatttc atttagctca
     1261 ctaaatccaa tgtctaaata tatttcctta ttgttaccta tcgcagatat tgctatatct
     1321 acaattctgt catctatttt ttttaagttt tctagtatgt cttttttcct tttttgtgtt
     1381 agtaggctag atacatttgt aattgttgcc tctttgttta tctttctgga tgttgttatt
     1441 attgttgctg tcttaaattt ataattaatc ccatcccttt cgttttttat ttcggatttt
     1501 atttctacgc catcttcttt caattgcata gagaaaagct tattggaaac cctgtttttc
     1561 gcattgggtg tctcggtcac atttattttt aaagagttta ctgtggcctg agaaccgata
     1621 gtttgacgag gtatgttttg gttagatagt acggtgtcaa cgtttagaga caaacttgat
     1681 tctaatctct gatttgtttc tgtttcatat gtttgtgtta cttcagagcg ttcatctcga
     1741 atgactatgg acatcttttg tgatgtgtcc atatcatgaa agtaactaac ccaaaatggc
     1801 ttgtttttgt ttatttctgc catttctttt ctgaatgctt gaatgtttaa caaaacagcc
     1861 gggtttttta catcataaaa acaaaaaata gcatccagta tagatgtttt cccgtagttg
     1921 tttttcccag ctatgatatt taaagtcttt aattgcttta gatatagctc attgaagcat
     1981 ttgaagttct ttaaacttat ttcctttatc atgatagcct ctgttcgtgt ttttgttata
     2041 tgccatacga tttgttagta ccttaataaa atgtacagct ttcattgtga ttagctagac
     2101 tttatgtcaa agattaactt tgattgcttg tgttttaatc gatttttttt actttcttct
     2161 tgagattatc tggcagcagc cgtaagcttc cggctgtgaa cgaaaaaaac cgccttggcg
     2221 ggcggttttt tcgaaaggtt cagtagagtt ggcgctctat gaaccgaggt aactggctta
     2281 aagggagcgc agtcaccaaa actgttcgtt tagtttagct ttaactggcg tataacttca
     2341 agactaactc cctaaatcag ttaccagtgg ctgctgccag tggcgctttg tcgtgtcttt
     2401 ccgggttgga ctcaagacga tagttaccgg attaggcgca gcagtcggac tgaacggggg
     2461 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     2521 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     2581 ggagagcgca cgagggagcc atcaggggga aacgcctggt atctttatag tcctgtcggg
     2641 tttcgccacc actgatttga gcgtcagatt ccgtgatgct cgtcaggggg gcggagccta
     2701 tggaaaaacg gccgctgtgc ggcctttcct tctttcctcc atgcgattac tcggttttct
     2761 cgagcgtttc tacgcatgct cctgccgctc gccgcagccg aacggcagga gcgtagcgac
     2821 tgagtgagcg aggaagcgga aaagaggaga tccctacact gaccactgac gctctgaggc
     2881 ctcttatgac gaaacaacag aaaacagcgc tgaacatggc taaattcatc cagaatcagt
     2941 ctctgctcct gctggagaag cttaacgagc ttgatctcga tgtcgaggcc gacctttgtg
     3001 agaaactcca cgatgatgcg gagcatcttt tccgtacact gtcctcgaga ctggatgcat
     3061 tacaggatgg taactgatgg ccagagtgtc tattagtgag gctgcccgac tgacgggtaa
     3121 gagtagaaca acactgcaca ggttaattaa agcaggtgac ctgtccacct gtagcggtga
     3181 gcgcaatgca aaaatggtag atacttcaga gcttttacgt gtctttggcc cctttgagca
     3241 accgaagggt gaacaccatt ctggacaggt ctctggacag cgtgatacag cctctgctga
     3301 ccagagtgaa caggtgatcc gtcatctgaa acaggagatt gaacacctga acaccttact
     3361 tttgtccaag gattcgcaca ttgacagcct gaaacaggcc atgctccttc ttgaaaatca
     3421 ccaggcaaaa ttaactgaat ccccggcgcc atggtggaaa ttctggaaaa aatcctgacc
     3481 ccgtcatgct tcagttttcc tctccggcca tcccagggag ttcctgaacc ttcccaaaaa
     3541 tcgtcagatc tctgaacgct cctcaccact aaaaataccc acaccccacg caaaattaaa
     3601 attttgcaga ttccttattt acaacaatgg gttaatgaaa aataaatgtg cggtgatgtg
     3661 ttacaaagtg cagatccaag tcgtggacga ctctatggcg aatcgctgac gaattgccca
     3721 cgattcatat gtgaatcacg ctttattatc atattgttat gatgaatcga ctcttgattc
     3781 aattcatcac tttgggtgtt ttttgctcat ggtgactctt gattcaattc acgattcgaa
     3841 tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat tgccggccgt
     3901 cgacgaaccc gattcctgaa ggtgctgaaa accggttttc cggctgcgcc ggcaacgctt
     3961 cacggccacg caataaattg cgtccccgtt caccgttttc ggaggagaag aacgccggaa
     4021 acaggacttc ccctggctga atggtgtgaa tttccgcgct atgcacttgc gcgcatactc
     4081 atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc
     4141 tgttttaccg gtttcccgtc agaaacg
//
