LOCUS       NZ_CP138687             4166 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 4254 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP138687
VERSION     NZ_CP138687.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040180
            Assembly: GCF_033847565.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 16:33:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,824
            CDSs (total)                      :: 5,699
            Genes (coding)                    :: 5,531
            CDSs (with protein)               :: 5,531
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 73 of 168
            Pseudo Genes (incomplete)         :: 103 of 168
            Pseudo Genes (internal stop)      :: 35 of 168
            Pseudo Genes (multiple problems)  :: 34 of 168
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138687.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="4254"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-02"
                     /collected_by="He Fang"
     gene            complement(265..708)
                     /locus_tag="R8537_RS29090"
                     /old_locus_tag="R8537_29090"
     CDS             complement(265..708)
                     /locus_tag="R8537_RS29090"
                     /old_locus_tag="R8537_29090"
                     /inference="COORDINATES: protein motif:HMM:NF022971.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_311975753.1"
                     /translation="MFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGNTKIS
                     FFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPTNEKK
                     AKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(693..896)
                     /locus_tag="R8537_RS29095"
                     /old_locus_tag="R8537_29095"
     CDS             complement(693..896)
                     /locus_tag="R8537_RS29095"
                     /old_locus_tag="R8537_29095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228263081.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKKVFLLY"
                     /name="hypothetical protein"
     gene            complement(896..2011)
                     /locus_tag="R8537_RS29100"
                     /old_locus_tag="R8537_29100"
     CDS             complement(896..2011)
                     /locus_tag="R8537_RS29100"
                     /old_locus_tag="R8537_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2356..2469)
                     /locus_tag="R8537_RS29105"
                     /old_locus_tag="R8537_29105"
                     /pseudo=""
     CDS             complement(2356..2469)
                     /locus_tag="R8537_RS29105"
                     /old_locus_tag="R8537_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2885..3076
                     /locus_tag="R8537_RS29110"
                     /old_locus_tag="R8537_29110"
     CDS             2885..3076
                     /locus_tag="R8537_RS29110"
                     /old_locus_tag="R8537_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3076..3477
                     /locus_tag="R8537_RS29115"
                     /old_locus_tag="R8537_29115"
     CDS             3076..3477
                     /locus_tag="R8537_RS29115"
                     /old_locus_tag="R8537_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3823..4065)
                     /locus_tag="R8537_RS29120"
                     /old_locus_tag="R8537_29120"
     CDS             complement(3823..4065)
                     /locus_tag="R8537_RS29120"
                     /old_locus_tag="R8537_29120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          complement(2357..2764)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 tcctgagtcc gttttagcgg tgcgtacaat tatgatttta tgttaaattg ttttaagtga
       61 atagagagaa tataacttaa gggagcatct tgtgctgggt cttttacatt agatctgcac
      121 aatcattgtt ttggcatctg gtgatgtctt taggacgttt gagattgctg ataactaaag
      181 tgtatgatgc tttctatacg ttatccggcg tttaataaca ccctggtgat aagccagccc
      241 gtgaagggct ggcttgattt ggatttatat ttttgaaata aaatctttta tttcatcaaa
      301 gcaattgtct gctgtgttta ttattccatt tttcatggat attcctattc ttgagtcgga
      361 ttctctattg cagctaagat agacttgtac cttagctttt ttttcgtttg ttggataagt
      421 gtactctttg tcactcgctg agatgtcatc tttattattt ttaatttttt cgaaaaattc
      481 tattatgtat tcatataacg ctcgagtgct tgcgtgttca attatcagat cttcaatggc
      541 accttgcttg tcttttcctg gcattataaa gaaagaaatt tttgtattgc cattcatagc
      601 tatctcgtta tgcgagctgg gggaggagat ttgtagttct cgcaatgtgg caattattga
      661 tctttctgtg tcagaaaatg atgtgtcagc atctaatata ataaaaatac tttcttttca
      721 tcgaaagaat caatgttttt gaatgctcta agtgcttgtt ttaattttga ttttccattt
      781 attttttcga caaaaacatc ggtaatgcct tgtatttcaa gtaggttgca tagaaaatga
      841 aaatcatcat caccctcaac gaaaatgatc tttttgctat tatttatgtc tttcattatc
      901 ttacctccca gtcgttctca actgagtagg agaaatcatc catattgaat tgtacggctg
      961 ttggttttag ggagttcttt tctcttccta gtctaatata agcgatatct tcatttttgc
     1021 tatctatttc gtttattgct cgtatcacat cttgactatg agttgttgca aatatttgat
     1081 tgttgttttg ttttgcagat gaaattaatg attttattat gtctttaatt actgagtaat
     1141 gtattccgtt ctcgatttca tcaataagaa taattgagtt ttcctgtacc agcactgagc
     1201 tgataaaaga taatgctcga ctgataccct cgccaagcat tgatatttca tttagctcac
     1261 taaatccaat gtctaaatat atttccttat tgttacctat cgcagatatt gctatatcta
     1321 caattctgtc atctattttt tttaagtttt ctagtatgtc ttttttcctt ttttgtgtta
     1381 gtaggctaga tacatttgta attgttgcct ctttgtttat ctttctggat gttgttatta
     1441 ttgttgctgt cttaaattta taattaatcc catccctttc gttttttatt tcggatttta
     1501 tttctacgcc atcttctttc aattgcatag agaaaagctt attggaaacc ctgtttttcg
     1561 cattgggtgt ctcggtcaca tttattttta aagagtttac tgtggcctga gaaccgatag
     1621 tttgacgagg tatgttttgg ttagatagta cggtgtcaac gtttagagac aaacttgatt
     1681 ctaatctctg atttgtttct gtttcatatg tttgtgttac ttcagagcgt tcatctcgaa
     1741 tgactatgga catcttttgt gatgtgtcca tatcatgaaa gtaactaacc caaaatggct
     1801 tgtttttgtt tatttctgcc atttcttttc tgaatgcttg aatgtttaac aaaacagccg
     1861 ggttttttac atcataaaaa caaaaaatag catccagtat agatgttttc ccgtagttgt
     1921 ttttcccagc tatgatattt aaagtcttta attgctttag atatagctca ttgaagcatt
     1981 tgaagttctt taaacttatt tcctttatca tgatagcctc tgttcgtgtt tttgttatat
     2041 gccatacgat ttgttagtac cttaataaaa tgtacagctt tcattgtgat tagctagact
     2101 ttatgtcaaa gattaacttt gattgcttgt gttttaatcg atttttttta ctttcttctt
     2161 gagattatct ggcagcagcc gtaagcttcc ggctgtgaac gaaaaaaacc gccttggcgg
     2221 gcggtttttt cgaaaggttc agtagagttg gcgctctatg aaccgaggta actggcttaa
     2281 agggagcgca gtcaccaaaa ctgttcgttt agtttagctt taactggcgt ataacttcaa
     2341 gactaactcc ctaaatcagt taccagtggc tgctgccagt ggcgctttgt cgtgtctttc
     2401 cgggttggac tcaagacgat agttaccgga ttaggcgcag cagtcggact gaacgggggg
     2461 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     2521 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     2581 gagagcgcac gagggagcca tcagggggaa acgcctggta tctttatagt cctgtcgggt
     2641 ttcgccacca ctgatttgag cgtcagattc cgtgatgctc gtcagggggg cggagcctat
     2701 ggaaaaacgg ccgctgtgcg gcctttcctt ctttcctcca tgcgattact cggttttctc
     2761 gagcgtttct acgcatgctc ctgccgctcg ccgcagccga acggcaggag cgtagcgact
     2821 gagtgagcga ggaagcggaa aagaggagat ccctacactg accactgacg ctctgaggcc
     2881 tcttatgacg aaacaacaga aaacagcgct gaacatggct aaattcatcc agaatcagtc
     2941 tctgctcctg ctggagaagc ttaacgagct tgatctcgat gtcgaggccg acctttgtga
     3001 gaaactccac gatgatgcgg agcatctttt ccgtacactg tcctcgagac tggatgcatt
     3061 acaggatggt aactgatggc cagagtgtct attagtgagg ctgcccgact gacgggtaag
     3121 agtagaacaa cactgcacag gttaattaaa gcaggtgacc tgtccacctg tagcggtgag
     3181 cgcaatgcaa aaatggtaga tacttcagag cttttacgtg tctttggccc ctttgagcaa
     3241 ccgaagggtg aacaccattc tggacaggtc tctggacagc gtgatacagc ctctgctgac
     3301 cagagtgaac aggtgatccg tcatctgaaa caggagattg aacacctgaa caccttactt
     3361 ttgtccaagg attcgcacat tgacagcctg aaacaggcca tgctccttct tgaaaatcac
     3421 caggcaaaat taactgaatc cccggcgcca tggtggaaat tctggaaaaa atcctgaccc
     3481 cgtcatgctt cagttttcct ctccggccat cccagggagt tcctgaacct tcccaaaaat
     3541 cgtcagatct ctgaacgctc ctcaccacta aaaataccca caccccacgc aaaattaaaa
     3601 ttttgcagat tccttattta caacaatggg ttaatgaaaa ataaatgtgc ggtgatgtgt
     3661 tacaaagtgc agatccaagt cgtggacgac tctatggcga atcgctgacg aattgcccac
     3721 gattcatatg tgaatcacgc tttattatca tattgttatg atgaatcgac tcttgattca
     3781 attcatcact ttgggtgttt tttgctcatg gtgactcttg attcaattca cgattcgaat
     3841 cgccggtgaa tcgtgtggtg agtcgtccgg cgacttgcca taacgtgatt gccggccgtc
     3901 gacgaacccg attcctgaag gtgctgaaaa ccggttttcc ggctgcgccg gcaacgcttc
     3961 acggccacgc aataaattgc gtccccgttc accgttttcg gaggagaaga acgccggaaa
     4021 caggacttcc cctggctgaa tggtgtgaat ttccgcgcta tgcacttgcg cgcatactca
     4081 tgcatgccgt aaaaacagag cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct
     4141 gttttaccgg tttcccgtca gaaacg
//
