LOCUS       NZ_CP138686             4842 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 4254 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP138686
VERSION     NZ_CP138686.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38040180
            Assembly: GCF_033847565.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Hao,G.
  TITLE     Identification and global prevalence analysis of Klebsiella spp.
            containing the tmexCD-toprJ gene isolated in Chinese clinics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Hao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, hangzhou, zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 16:33:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,824
            CDSs (total)                      :: 5,699
            Genes (coding)                    :: 5,531
            CDSs (with protein)               :: 5,531
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 73 of 168
            Pseudo Genes (incomplete)         :: 103 of 168
            Pseudo Genes (internal stop)      :: 35 of 168
            Pseudo Genes (multiple problems)  :: 34 of 168
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138686.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="4254"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="28.9063884 N 120.1962952 E"
                     /collection_date="2021-02"
                     /collected_by="He Fang"
     gene            complement(join(4797..4842,1..128))
                     /locus_tag="R8537_RS29050"
                     /old_locus_tag="R8537_29050"
     CDS             complement(join(4797..4842,1..128))
                     /locus_tag="R8537_RS29050"
                     /old_locus_tag="R8537_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016241875.1"
                     /translation="MIIAIGMMLLCIMLGGFMIRSCMGKTNSSSEAKQSISDVRKIIDK
                     KELIKYKRRNQR"
                     /name="hypothetical protein"
     gene            complement(148..399)
                     /locus_tag="R8537_RS29055"
                     /old_locus_tag="R8537_29055"
     CDS             complement(148..399)
                     /locus_tag="R8537_RS29055"
                     /old_locus_tag="R8537_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016241876.1"
                     /translation="MKIVLSVSKCLYINDLDRLLPCDRFFVHLDYKDISKNESFEMNRH
                     DKESYKLIIDKIVGNEVMLAIDEDIYSEVVENLTLLLK"
                     /name="hypothetical protein"
     gene            complement(1016..1231)
                     /locus_tag="R8537_RS29060"
                     /old_locus_tag="R8537_29060"
     CDS             complement(1016..1231)
                     /locus_tag="R8537_RS29060"
                     /old_locus_tag="R8537_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224373721.1"
                     /translation="MQKTKRRLGFMVGKSNATLPPDDETEAELDQEVLKLFYPNQETQD
                     TIERSEAGTDVFMASGSDDLFKKLKL"
                     /name="hypothetical protein"
     gene            1532..2398
                     /locus_tag="R8537_RS29065"
                     /old_locus_tag="R8537_29065"
     CDS             1532..2398
                     /locus_tag="R8537_RS29065"
                     /old_locus_tag="R8537_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_057483990.1"
                     /translation="MFKIENNKDILKSQMPLKPYATNDLEFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMIFDLDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPITSTEKSR
                     QKPVKFARALYYNMATRLGADLGYAGLITKNPLNPHWSPYWSDADLYELNDLADCFDDL
                     EDPKKRENTELAFGRNVEIFDTQRQWAYKNVLKYKNESSFDAFYKELLLKCETFNSFAN
                     YNNLLPYKELVSTAKSIAKFCWKEFSNEKLHDIQSKKQKARRSKRTEKQKLHTKTLLEK
                     AIMINEE"
                     /name="replication initiation protein"
     gene            2402..2677
                     /locus_tag="R8537_RS29070"
                     /old_locus_tag="R8537_29070"
     CDS             2402..2677
                     /locus_tag="R8537_RS29070"
                     /old_locus_tag="R8537_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="WP_224373719.1"
                     /translation="MTALHTVKRNKTAKEMAQRLNISDRTVRKLIALPREDYEKEAKQR
                     RLKAFVLRESGMKWADVGIALGTTKHGAIALYKRYQQIDAPTTKEA"
                     /name="HTH domain-containing protein"
     gene            complement(2686..4176)
                     /locus_tag="R8537_RS29075"
                     /old_locus_tag="R8537_29075"
     CDS             complement(2686..4176)
                     /locus_tag="R8537_RS29075"
                     /old_locus_tag="R8537_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_301717673.1"
                     /translation="MKGNVAKPGRSFKNRVDYILKDDHDFICSNMASDYNNVSDLTDEF
                     KTVSSFRQDIKKPVFHAFLSLPKNEHLTDEQWQEIAKDYLKEMNIDIDKHQYICVRHND
                     TDKEHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHGLTITDGLKGQKSEVSAPT
                     KNEIEQALRTGEKPARIVLQNALQAAMVGKPDLETFIDRLQAVGIEAAFNVASTGNVAG
                     VSFGIKNNEKNIYFKGSQLGKKYSWNTIKNKVIYDKNRDDELVRRFAARKTDDENSIRR
                     SPTEPDFNDSRAVDRIDDDTIGVSQHGIKPDTTDTNQSEQLIRSNNNRFKLSEKYNRPA
                     SHKVRRTTQKAAQILNRVKKQYNKEPIKTYNKNGLNSWRDKSDSGSIGRILDHAETANA
                     ATHSFRLKGRGSFNSRATKIEEIRDKLGIKKPVIQPKNKEKVIEKEIKKKPLGLSMNEN
                     RFKSKLKEALKKEKPKNETTPLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4173..4544)
                     /gene="mobC"
                     /locus_tag="R8537_RS29080"
                     /old_locus_tag="R8537_29080"
     CDS             complement(4173..4544)
                     /gene="mobC"
                     /locus_tag="R8537_RS29080"
                     /old_locus_tag="R8537_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_064374444.1"
                     /translation="MKTDRKRGRIELNHEVRRDISIQFRVNKAEFEYLKELVEASGLER
                     GHYIRREILNSEPIVKRKMPDFEQSLYKEIQHQGNNINQIAKKLNTNEDFKQSVAAEAN
                     FAIKQLMKRILKACEVYTI"
                     /name="mobC"
     gene            complement(4531..4800)
                     /locus_tag="R8537_RS29085"
                     /old_locus_tag="R8537_29085"
     CDS             complement(4531..4800)
                     /locus_tag="R8537_RS29085"
                     /old_locus_tag="R8537_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_150316402.1"
                     /translation="MIMEYEMKLNVLARFFYYIEQAKDIPFDYSSYDEQGLCYFVANRY
                     INENKADDLIQALIDTNDDDYIKAIRDYVQYTALNEVRKKYEDR"
                     /name="hypothetical protein"
ORIGIN
        1 attatttttc taacatctga tattgattgc tttgcttcac ttgaagagtt tgtttttccc
       61 atacaactac ggatcatgaa accaccgagc ataatacaaa gaagcatcat tcctattgct
      121 ataatcatat attaatctcc ataagcctca ttttaataat aaagttaagt tttcgacaac
      181 ttcgctatat atatcttcat caatagcaag cattacttca ttacctacaa tcttatcaat
      241 gataagtttg tagctttctt tgtcgtgtct attcatctca aatgactcat ttttgctaat
      301 atccttataa tctaaatgaa caaaaaaacg atcacacgga agtaagcgat ctaaatcgtt
      361 tatgtataaa cattttgata ctgataaaac tattttcata tttatttctc ctatatttat
      421 aatatctaac tatggtttac cgcttcccct tgatagggga aataagcgga agggtagatc
      481 tttttcaacc ttgcgagtga aacgagacaa ggcaatccga taggattggg taaaatagcg
      541 gatacgctat tacaccttgc ctcagtttcc gaaaagataa aaataaagct gaactcaacc
      601 tatatttaaa taaataaccg atctaaaaag tttgtcaata tagcggctac ataaataaat
      661 accctgcaca acaaataaaa tgcaaataaa aaatagggta aataatatat tgtcttgatg
      721 ggtagctgtg cgtaataggt agctttgcgt aaaaaatgcc gctgctacgt gaaatctgga
      781 aaagttcgtg accacttggt aggcagattt taaggaatgg ggtttgatcg gcggcgacag
      841 aacagtagaa aactcgcttt tctgctgttg ctggcgtcta tgtgtttgaa atgctcataa
      901 aatcaacaga ctatagtata tattagtata gtatatactg catcgagggt tggccttctc
      961 tcaccgttcc gggtcggttt gttctactgg tgaagatggc cggctctccc ctgccctaca
     1021 gcttcagttt tttgaacagg tcatcagaac cggaggccat gaaaacgtct gtaccggctt
     1081 ctgagcgttc tatagtgtct tgtgtctctt ggttaggata aaacagcttc aggacttcct
     1141 gatcaagctc tgcttctgtt tcgtcatctg gtggcaatgt tgcattgctc ttaccaacca
     1201 taaagccgag tctacgcttt gttttctgca tatcatgctc tcctatccag cacagccaga
     1261 tcttacatag agtttagtat acgttacagt gtgacttact ctgtagatgg ccttcggcct
     1321 gtgcttcgct gtggtgtagg atccccaaaa ctccgtctaa tacctgatat tggtagactt
     1381 cctacttaac aacttttttt gacggatcta ttgacaagat attttgatat agtaaaactg
     1441 taagagatta taaataaaag aaaagcccgc gttctctgat tagtttggcg accagtgaga
     1501 gaacggggca ttacagaaag gatactgcta tatgttcaag atagaaaata acaaagatat
     1561 attaaaaagt caaatgcctt taaagccata tgctacaaat gatttagagt ttggattgac
     1621 tattcaaaat aagaataaag ctttagatat gctttatcta caagtaaatc acccgcttta
     1681 tcttcataca atgatatttg atttagacaa agaaaactgc ttttacgagt ttgaaaatgc
     1741 ccatttacct ataccgtctt ttataactaa aagccctgat agtggacgtt gccactacgg
     1801 ttatatgctt aatgctccta taacatcaac agagaaaagc agacagaagc ccgtaaagtt
     1861 tgccagagca ttatattaca atatggctac tcgtcttggt gctgatctgg gttatgcggg
     1921 actgattaca aaaaacccgc taaatcctca ttggtcgcct tattggtcag atgctgattt
     1981 atatgagcta aatgatttag ctgattgttt tgatgatttg gaagacccaa agaaaagaga
     2041 aaatacagag ttagcttttg gtcgcaatgt tgaaatattt gatacacaaa ggcaatgggc
     2101 ttataaaaac gtgctaaaat ataagaatga aagctcattt gatgcatttt ataaagagtt
     2161 attattgaaa tgcgaaacat ttaatagctt tgctaactat aataatctat taccttacaa
     2221 agaacttgta tcaacagcaa aaagtatagc caagttttgc tggaaagaat ttagtaacga
     2281 aaaacttcat gatattcaga gtaaaaaaca gaaagcaaga agatctaaaa gaacagaaaa
     2341 acaaaagcta cacactaaaa cacttttaga aaaagcaata atgattaacg aggagtaaaa
     2401 tatgacggca ttacatacgg taaaaaggaa taaaacagcc aaagaaatgg ctcaaagatt
     2461 aaatatcagt gatagaaccg tcaggaagtt gattgcttta cctcgtgaag actatgagaa
     2521 agaagcaaaa caaagacgat taaaagcctt tgtattacgt gaaagcggga tgaaatgggc
     2581 agatgttggt attgctcttg gaactacaaa acacggtgct atagctcttt ataaacgtta
     2641 tcaacaaata gacgcaccaa caacaaaaga ggcttaaagc ctcttttatc gcggtttaaa
     2701 acctcttggt tttaaatgct gatcgtcttc ctgctgtagt ggtgttgttt cattttttgg
     2761 tttttctttt ttaagagctt cttttaactt cgatttaaat ctattttcat tcatgcttaa
     2821 tcctaaaggt tttttcttta tttctttttc aatcactttt tctttattct ttggctgaat
     2881 aactggtttt tttattccaa gtttatctct tatctcttcg atcttagttg ctctactatt
     2941 gaaactgcca cggcctttga gtctaaatga atgggtggct gcgttggctg tttctgcatg
     3001 atcaagtatc ctaccaatag aaccgctatc agatttatca cgccaactat tgaggccatt
     3061 tttattataa gttttgattg gttctttatt gtattgtttt ttaactctgt tgagtatttg
     3121 tgcagctttt tgtgttgttc ttctaacttt atggcttgct ggtctattat atttttcgct
     3181 gagtttaaag cggttgttgt tgcttctaat gagttgttca ctctggttag tgtctgttgt
     3241 gtctggttta atgccgtgct gactgactcc gatagtatca tcatcgattc tgtcaacggc
     3301 tctactgtca ttgaaatctg gttcagtagg tgatcgtcta atactatttt cgtcatctgt
     3361 ttttcttgct gcgaatcttc taacaagttc atcatctcta tttttgtcat atatcacctt
     3421 gttttttata gtgttccaac tgtatttttt acccaactga gatcccttga aataaatatt
     3481 tttttcatta tttttgatac caaacgacac cccggcgacg ttgccagtgc ttgcaacgtt
     3541 aaaggccgcc tcgatgccta cagcctgcag tctgtctatg aatgtctcaa gatctggttt
     3601 tcctaccata gccgcctgta atgcgttctg tagcacgata cgagctggtt tctcacctgt
     3661 cctcaaggct tgctcgatct cgttcttggt tggtgctgat acctctgatt tttgaccttt
     3721 taagccatcc gtgatggtta agccgtgttt tacttctaaa cgttcacaag ctgctattgt
     3781 gttaaatgct gagtgctgac cgagccatac agaaccatca aggccaacac gattagctac
     3841 aatatggata tgttctttat ctgtatcgtt atgacgaaca caaatatatt gatgtttgtc
     3901 tatatcaata ttcatttctt tcaaatagtc ttttgctatc tcttgccatt gttcatctgt
     3961 taaatgttca ttttttggaa gagataaaaa agcgtgaaat actggttttt ttatatcttg
     4021 tctaaagctg cttacagttt taaactcatc agttaaatca ctaacattat tataatcact
     4081 tgccatattg ctacatataa aatcgtggtc atctttaaga atgtaatcaa ctctattttt
     4141 aaatgatctt ccgggttttg ctacatttcc tttcatatgg tataaacctc acaagccttt
     4201 aatattcttt tcataagttg ctttatagca aagttagctt ctgcggctac agattgttta
     4261 aagtcttcat tcgtatttag ctttttagca atctgattta tattattacc ttgatgttgt
     4321 atttctttat acaatgattg ctcaaaatcc ggcatttttc ttttaacaat aggttcagag
     4381 tttaaaatct ctcttctaat gtaatgacct ctttcaaggc cagacgcctc aacaagttct
     4441 tttaaatatt caaactctgc tttgtttact ctaaactgta tagatatatc tctacgtact
     4501 tcgtgattta actctatacg tcctcttttt ctatctgtct tcatatttct ttctcacttc
     4561 atttaaagct gtatactgaa cataatctct tatagctttt atataatcat cgtcattagt
     4621 atctatcaat gcctgtataa gatcatcagc tttattttcg ttaatgtatc tattagctac
     4681 aaaataacat aatccttgtt cgtcatagct tgagtaatca aaaggtatat ctttagcttg
     4741 ctcgatataa taaaaaaaac gtgctaacac attaagcttc atttcatatt ccataatcat
     4801 ctttggttcc ttcttttgta tttaatcagt tcttttttat ct
//
