LOCUS       NZ_CP138646             5960 bp    DNA     circular CON 21-NOV-2023
DEFINITION  Enterococcus faecalis strain ES-108-1 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP138646
VERSION     NZ_CP138646.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38203341
            Assembly: GCF_033814055.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Yoon,J.W.
  TITLE     Complete genome sequences of Enterococcus faecalis isolated from
            pigs in South Korea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Yoon,J.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) Department of Veterinary Medicine, Kangwon
            National University, 1 Gangwondaehakgil, Chuncheon, Gangwon 24341,
            South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: De novo assembly using Unicycler v. 5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 474x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/21/2023 09:40:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,314
            CDSs (total)                      :: 3,229
            Genes (coding)                    :: 3,152
            CDSs (with protein)               :: 3,152
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 39 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 10 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138646.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="ES-108-1"
                     /isolation_source="Pig rectum feces"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed4"
                     /geo_loc_name="South Korea:Gyeonggi-do, Anseong-si"
                     /collection_date="2021-07-05"
                     /collected_by="Nguyen Thi Mai Tho, Jun Bong Lee"
     gene            1..723
                     /locus_tag="SHT68_RS16520"
                     /old_locus_tag="SHT68_16515"
     CDS             1..723
                     /locus_tag="SHT68_RS16520"
                     /old_locus_tag="SHT68_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002360724.1"
                     /translation="MSDKSELAVKYQNELNLVPLKNFNAKQMDLFFALCARMKDKGVEN
                     IRFSFEELKELSDYKMTATKAFVADLEKLYKDMLNLSYRTENDDEIEYFVLFNGFKIDK
                     KQKFVEVRVNQDLDYIINGLTTEFSRFELSAFTSIRSTYAKTLFRLLMQYRSTGYYVVS
                     IEDFRELLDIPEYYQMGNIDQKVLKPAMKELHNYFENLEVTKIKAKKGNKIAKLEFTFT
                     GLKTNKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            850..1146
                     /locus_tag="SHT68_RS16525"
                     /old_locus_tag="SHT68_16520"
     CDS             850..1146
                     /locus_tag="SHT68_RS16525"
                     /old_locus_tag="SHT68_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002360723.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLERLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLIDTVAYKVTLEEDISCGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            complement(1808..2488)
                     /locus_tag="SHT68_RS16530"
                     /old_locus_tag="SHT68_16525"
     CDS             complement(1808..2488)
                     /locus_tag="SHT68_RS16530"
                     /old_locus_tag="SHT68_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            2741..4420
                     /gene="mobV"
                     /locus_tag="SHT68_RS16535"
                     /old_locus_tag="SHT68_16530"
     CDS             2741..4420
                     /gene="mobV"
                     /locus_tag="SHT68_RS16535"
                     /old_locus_tag="SHT68_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_010717414.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            4509..4709
                     /locus_tag="SHT68_RS16540"
                     /old_locus_tag="SHT68_16535"
     CDS             4509..4709
                     /locus_tag="SHT68_RS16540"
                     /old_locus_tag="SHT68_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            complement(5228..5314)
                     /locus_tag="SHT68_RS16545"
                     /old_locus_tag="SHT68_16540"
     CDS             complement(5228..5314)
                     /locus_tag="SHT68_RS16545"
                     /old_locus_tag="SHT68_16540"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     Typing          complement(1379..1638)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="81.0"
                     /evalue="1.95e-45"
     Typing          2..723
                     /name="rep_cluster_2301"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2667..4417
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.829"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtgata aatcggaatt agctgttaaa tatcaaaatg agttaaatct tgtgccactt
       61 aaaaacttta atgcaaaaca aatggatctg ttctttgcgt tatgcgcacg aatgaaagat
      121 aaaggagtag agaatattcg tttctccttc gaggaattaa aggaattgag tgactacaaa
      181 atgactgcta caaaagcctt tgtagcagac ttagaaaagt tgtataaaga tatgctgaac
      241 ctttcttatc gtacagaaaa cgatgacgaa atagagtatt ttgttttatt caatggattt
      301 aaaatcgaca aaaaacaaaa gtttgttgaa gttcgagtta atcaagattt agactatata
      361 ataaatggtt taacaacaga gtttagtagg tttgagctgt ccgcttttac ttcaatacgc
      421 tcaacatatg ctaaaacatt gtttcgtttg ttaatgcaat atcgttcaac agggtattat
      481 gtagtcagta ttgaagactt cagagaattg ttagacatac ccgaatatta tcaaatgggt
      541 aacattgacc aaaaggtttt aaaaccagca atgaaagaac tccataacta ttttgagaat
      601 ttagaagtaa caaaaatcaa agccaaaaaa ggcaataaga ttgccaaact agaatttacg
      661 tttactggtc tgaaaaccaa caaaccaagc gtaaccatgc acgattgggt aaacggagaa
      721 taaaaaaaga acttgccccc tcacattaag caagttagca agttcctcac gcaatcttcc
      781 ggactgctag attattttag catatttgcg tgaggacttc taaaataaat tattttggag
      841 gtcctactta tgggattaat gacaggatac aacatgacaa aagaggagta cgaagtcaca
      901 caggctgtac tggagcgact agataagttt gagagtgaac tagaagcgct attgcttgat
      961 ttggagccac ttgacttatc acgattagat actgtagttc gagacgaaac gctaagaatg
     1021 tttaattatc taagtcggtc atacattcta cttattgata cggtagccta caaagttacg
     1081 ctagaagaag atatttcctg tgggattgtg aagattgacg gtcaatctct agacgaagaa
     1141 gaataaaaac tacccttgct tgttataaaa aaatgttgac tatttgggat aacactcgaa
     1201 aaatatcaag attagcctac ttccattttg gggtaggctt tctttttgca agcggagcaa
     1261 catggtacca tgttgcgata ttttgaacga ctcctaccaa aaatactggt aggagtcgtt
     1321 caaatttttg gggttccccc aatcccactg ctgttatttt ttgcttttac tgttgaaatt
     1381 cgcttaattt cttcctgtgt ttcggaagag cataagggga actggggggc acacaaggta
     1441 ccccgccttg cagtgcaagg ctttcccgtg gggtacactt tcgttcttcg ctagcacttt
     1501 actagcaggt actttcaggt aaactagcac atttttagca ctcaatagca ggtttctagc
     1561 acccaaacta gcagattcct agcagtgact agcaggtttt tagcaccaaa ttagcacatt
     1621 actagcaccg gctagcagaa agctagcacg ttcactaatt tcaggcagtc gttatttaaa
     1681 cggttttctg acggagggaa atttagagtt tctgccttag cagaaatgga aagtttgacc
     1741 gtaggcaatc ggcggttctg ttgcaaagtt ttaaataaag aataaaatcc cttacggtat
     1801 ctatgattta agctgggatt cccaataata ccttgatttc agtacagacc gaaaacccga
     1861 agagagtgcc ttcttttcgg gttttcttat ataatcctcg aatggcttcc atgcctttaa
     1921 tcgtggtaga ggcagtgcgt aaacttcgat agaatttatt gcgtctcttt actggacgat
     1981 ggtcttgttc aatcaaatta ttcaggtatt taatggtacg atgttctgtc ccttgataaa
     2041 agccgtattc ttttagtttc ttaaaggcac ttgtaataga gggggcttta tctgtgacta
     2101 caaccttcgg ttcatcaaac tgcttcacta accgcttaag aaaagcatag gctgcttgtg
     2161 tgtcccgttt tttacgtaac caaatatcca aggttaaacc atctgcatcg atggctcgat
     2221 acaaataatg ccattttcct ttaattttga tgtacgtttc atccattttc catgaataaa
     2281 aggatttttt atttttcttt ttccaaattt gatagagtag tttgccatat tcttgcaccc
     2341 aacgataaat cgtcgtatga gaaacgttaa tgccacgatc atataagatt tcttgaactt
     2401 cacgatagct aaggttataa cgaagatagt agcccacggc tacaataatc acatcctgct
     2461 gaaattgctt tcctttaaaa tgattcatcg tcattcctcc tgctatcttt ttctattatt
     2521 ctaccttatt tgatagtaga tttaaaactt tgcaacagaa cccaatcggc taagtgcgag
     2581 attgggaagc ctaaaggctg acctttcgag tactagccga cagccctcgg cagagcgcac
     2641 ctttacgaag taaagtatag tgcgttatac tttacttcga agtactgccg aaatccaaag
     2701 acaccgaacg acacaagaaa aaaaaggggg aggagcgctc atgtcaaaaa ttgttgcacg
     2761 tatggaaaaa atgaaagacg gaaatttaag tggtatccaa cgacataatc aacgagaaac
     2821 caataatcat tccaatcctg atattgatat tgagaaatct cacttgaatt atgacttagt
     2881 caatcctggt tcaatcaatt atcgggagaa aatcaaacaa atcattgaga gccaacgaat
     2941 cagtaaacga gcggttagaa aagacgcagt ccttgtgaac gaatggataa tcactagtga
     3001 taccgccttt tttcaagaga atacagacac acaagcattt tttaccgatg ttgtcgcata
     3061 tttctctgat cgctgcggtc gacaaaatgt cgcctatgcc acggtacatt tagacgaaac
     3121 cacgccccat atgcacttag gaattgtgcc tatgtacgaa gggcgattga gcagtaaaca
     3181 ggtgtttagt cggcaaaatt tgttagagat tcaagaagaa ttgccaacct atttgcaaga
     3241 acgaggctat gctattgagc gtggactccg tgggagtccg caaaagcatt tatctgtcaa
     3301 agaatacaaa gacgttcaaa aggagctaca agtttctaag caggaactcg ctaatcagga
     3361 acaaattctg gcagaaaaag agagagaact ccattcttat gaacaggcaa tcttccgtat
     3421 tgaccgcctt cttgaagaga agaacgaaga agtaaagggc aaagtccaag caatgaacca
     3481 actggaaacg aagcgaaacg aaatcattaa ctctctacaa acaaccgttt tacctgattt
     3541 agcaaatgtc aaagaaaaga aatttagttt aaacggttta aaatacgttt taagcgcttc
     3601 tgacttttcg gcgataaaaa gtattacgaa agatttcgag acgcttagaa cgcaaaataa
     3661 gcaccttaaa atcgaaaaca ataacttggc acagaaaaat aaagaattga cacaagtaaa
     3721 tcatgaacaa gaattacaaa taacggaatt agcctatgaa ttaaccctga aaaaagaaca
     3781 attaacagcg tttgaagaaa aacaactaaa ctatgaccag agcgaacaaa tcaaacaaga
     3841 acttgataca acaacacaaa cagtttctgt gttagaaaca gagaataagc ggttaaaaga
     3901 taaaattact gacttaacct acgacttttc tgaactaaat gaaaaatatc aagaaataaa
     3961 attaaagttt gacgggttaa ctacttacct tcacgaaaga ctaggtcatg ctaaagaaac
     4021 cattttattt acgattcagc aaggagtgaa gtaccttcag aacgcgccta aattaaaaaa
     4081 atccctagag cgatattcca aggatagaac ttacctcatt gacgaaaaag ggacattgtt
     4141 tttaccacca gatacggggt caaaaggggt aacaatgtcc ttaaacccac atttctaccc
     4201 cctaaaagcc ccgttttaca acagagaaac agggtgttat gagtttgtcg gcgaatatat
     4261 ggctaatgga cgttctcgaa cgaatacttt acaactgtct gaagaacaag tgctagagaa
     4321 gaaagctttt acgtttgaac agtcgacaga taaaaaggct tggcaacaga gtaaaaaata
     4381 ttcaagagat atgtcaaaag gacgaggttt atctcgttaa aaataattta attgataaat
     4441 tattgacaca taaaaacagt taaattaaaa tatattaata aaaattaaaa aaaagtgagg
     4501 taagcattat gaaaaagttg ttagttctaa ttttaggggt gtcttttatc tctgttgctt
     4561 taagttcaac tacagaagca agtgctagag agtgggattc tatagagtgt cagccaggat
     4621 tttattggaa aaaaggcggg tggactgcag gaggatatca taaagccggt ggattttgta
     4681 tcccaatgca tacaagatgg acaaaatagt tgctaaattt gaggttattt catgaaaaaa
     4741 tatctaataa aaaatatatt ttatattact attcctttag ttttaagcta tataacttct
     4801 tttttagtta atattgattt gcccatattg ataattattt tttacggaat tttattattt
     4861 tttttaattc cttcagaagt atatttggga agtacaatgg attacaacgc gaaagtagtg
     4921 aatcccactt acagaccaga aaataaaagc tttgaagata gtccaaaaag taaaatattg
     4981 tctatcctca tagtattact atgtttaata ctaactattt cgatatggta tttctctaat
     5041 tgacgttttg cgtaatctaa tctgatgttt gaaaatacag aaaaaaaatg ttattgtgta
     5101 agtgcgaagc gaaattaagc aaaatcatgt ttacaccaaa aagccctcct attctcagta
     5161 ggagggcttt tttacactgt tgcgatttca gcgttggagc tagaaaagca actgcataga
     5221 ttatctgcta atcgtctagc caacgatcaa ctagcaaaag tactaaaccc acaaaaagtg
     5281 gggcgataat caacgaaatt aagctttcaa tcatgtttac accccctttc ggggcagttg
     5341 ccaaaacaag tatatcacaa gaagaatttc ttgtgggggt tgttagggct tgccctgacc
     5401 gcctgcaagg cgcaatccta accaagatac cctaaaacaa ctaaacatgt cttaaagatc
     5461 ttcagacaag caaatagagc ctaaccaaat gatttacctt gtcaacggta ttgattttga
     5521 ttttagcgag tgaacgaagt gaacgctgca agcaaaatgt gagcgggatt ttcgctcact
     5581 ccttttttga ttttttagtg ccagttttgt aaatatcgcg gacctgtttg tagaaaaaag
     5641 aggttaattt tgggcgggaa atttccgcgc gcgcacgcgt ttcttttata tatattttat
     5701 atatattttt aaaacctttt taggcgggct gtgtgccacg tggctctaag gaagagcaga
     5761 tggtaccaac aactttttat ctgactacca acaacttttt atctgactac caacaacttt
     5821 ttatctgact accaacaact ttttatctga ctaccaacaa ctttttatct gttttatttt
     5881 cataatatta aaacaaacaa ccaactgttg acaaagttgg ttgtttgttt taatattcga
     5941 attgtgaggg ggcgaaagga
//
