LOCUS       NZ_CP138640             4847 bp    DNA     circular CON 21-NOV-2023
DEFINITION  Enterococcus faecalis strain ES-3-1 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP138640
VERSION     NZ_CP138640.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38203339
            Assembly: GCF_033811895.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Yoon,J.W.
  TITLE     Complete genome sequences of Enterococcus faecalis isolated from
            pigs in South Korea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Yoon,J.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) Department of Veterinary Medicine, Kangwon
            National University, 1 Gangwondaehakgil, Chuncheon, Gangwon 24341,
            South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: De novo assembly using Unicycler v. 5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 559x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/21/2023 09:44:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,883
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 32 of 49
            Pseudo Genes (internal stop)      :: 8 of 49
            Pseudo Genes (multiple problems)  :: 6 of 49
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138640.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="ES-3-1"
                     /isolation_source="Pig rectum feces"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed3"
                     /geo_loc_name="South Korea:Gyeonggi-do, Anseong-si"
                     /collection_date="2021-07-05"
                     /collected_by="Nguyen Thi Mai Tho, Jun Bong Lee"
     gene            complement(325..621)
                     /locus_tag="SHT66_RS14395"
                     /old_locus_tag="SHT66_14395"
     CDS             complement(325..621)
                     /locus_tag="SHT66_RS14395"
                     /old_locus_tag="SHT66_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033922262.1"
                     /translation="MGVMTGYNMTKEEYEVTQAVLTRLNKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLLDTVVYKDTLEEDIACGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            complement(749..1486)
                     /locus_tag="SHT66_RS14400"
                     /old_locus_tag="SHT66_14400"
     CDS             complement(749..1486)
                     /locus_tag="SHT66_RS14400"
                     /old_locus_tag="SHT66_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033922293.1"
                     /translation="MKEKTELAVKYQNKLNLVPLKNFNSKEMDLFFALCARMKDRGTEE
                     ISFTFEELRNLSDYKMTATKAFIKDLDSMYSKMLQLTYRENLFDDDDDGFRRFVLFTGF
                     EVSTKQQVVKVSVNSKLESVINGLTTEFSKFELAAFTSIRSSYAKTLFRLLMQYRSTGY
                     YQVNIEEFKEIMDVPKSYTQMGQIDQKVLKPAMKELNNYFEDLEITKIKAKKGNKIAKL
                     EFTFTGLKNNKPSITMHDWVNGE"
                     /name="replication initiation protein"
     gene            complement(2054..2371)
                     /locus_tag="SHT66_RS14405"
                     /old_locus_tag="SHT66_14405"
     CDS             complement(2054..2371)
                     /locus_tag="SHT66_RS14405"
                     /old_locus_tag="SHT66_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016388709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033922266.1"
                     /translation="MKTLYKKKLADVWEISKTNEQPEWVKQAFAKNYLRWTTDNRLVVL
                     MSALEPSVITNIKFGMVGSVGGGFAGYAMYSVGDIGDFLDATNHKIVSKKRFHKQYVIQ
                     G"
                     /name="hypothetical protein"
     gene            complement(2395..2637)
                     /locus_tag="SHT66_RS14410"
                     /old_locus_tag="SHT66_14410"
     CDS             complement(2395..2637)
                     /locus_tag="SHT66_RS14410"
                     /old_locus_tag="SHT66_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="WP_048941721.1"
                     /translation="MNQKIHTKRKAVNSTNKYKELSEKELKQTIGGGWGDFLGGLLGGL
                     APTRTAEQLNGSDYANFKARNCGPYGQGGTPNSCN"
                     /name="bacteriocin"
     gene            complement(2716..4395)
                     /gene="mobV"
                     /locus_tag="SHT66_RS14415"
                     /old_locus_tag="SHT66_14415"
     CDS             complement(2716..4395)
                     /gene="mobV"
                     /locus_tag="SHT66_RS14415"
                     /old_locus_tag="SHT66_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_319451442.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANIKEKKFSFNGLK
                     YVLSASDLSAIKSIAKDFETLRTQNKHLKIENNTLAQKNKELTQVNHEQELQITDLAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDRTTQTVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGQAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLENKAFTFEQSTDRKVWQESKNHSKGLSKNRGLSR"
                     /name="mobV"
     Typing          complement(2720..4470)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.918"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 acacgggaaa gccttgcagt gcaaggcgtc ccaccttgtg tgggacccag tttcccttat
       61 gctcttccga aacacaggaa gaaattaagc aaatttcaac agtaaaagca aaaaataaca
      121 gcagtgggat tgggggaacc ccaaaaattt gaacgactcc taccagtatt tttggtaggg
      181 gtcattcaaa atatcgcaac atggtaccat gttgctccgc ttgcaaaaag aaagcctacc
      241 ccaaaatgga agtaggctaa tcttgatatt tttcgagtgt tatcccaaat agtcaacatt
      301 ttttataaca agcaaggcta gtttttattc ttcttcgtct agtgattgac cgtcaatctt
      361 cacgatccca caggcgatat cttcttctag cgtatctttg tagacgaccg tatcaagaag
      421 tagaatgtac gaccgactta aataattaaa cattcttagg gtttcgtctc gaactacagt
      481 atctaatcgt gataagtcaa gcggctccaa atcaagcaat agcgcttcta gttcactctc
      541 aaacttattt agtcgagtca gtacagcctg tgtgacttcg tactcctctt ttgtcatgtt
      601 gtatcctgtc attactccca tgtgtatgac ctccaaaata atttatttta gaagtcctca
      661 cgcaaatatg ctaaaataat ctagcagtcg tagtggattg cgtgaggaac ttgctaactt
      721 gcttaatgtg agggggcaag ttcttttttt attctccgtt tacccagtca tgcatagtta
      781 tgcttggttt gttgtttttc agaccagtaa acgtaaattc tagtttggca atcttattgc
      841 ctttttttgc tttgattttt gttatttcta aatcttcaaa gtagttattg agttctttca
      901 ttgctggttt taaaaccttt tggtcaattt ggcccatttg tgtgtacgat tttggaacgt
      961 ccattatttc cttgaattcc tcaatattta cttggtaata tcctgtagag cgatactgca
     1021 taagcaaacg gaacaatgtt tttgcataac ttgaacgaat cgacgtaaag gctgcaagtt
     1081 caaacttact aaattcggta gttaagccat ttataactga ttcaagttta gaatttacgc
     1141 taactttaac tacttgctgt tttgtactta cctcaaagcc tgtaaataaa acaaaacgtc
     1201 tgaatccgtc atcatcgtca tcaaataaat tttctcggta agttagttgt aacattttac
     1261 tgtacatgct gtctaaatcc tttataaaag ctttagtagc agtcatcttg tagtcactaa
     1321 gattccgaag ttcttcaaaa gtaaatgaga tctcttcagt tccacgatct ttcattcgtg
     1381 cacataaagc aaaaaataaa tccatttctt tggagttgaa atttttcaaa ggcactaagt
     1441 ttaatttatt ttgatactta acagctaatt ccgttttttc tttcattcct ttcgcccccc
     1501 tcacagatat cactataaca aacactacct gcatagtcaa taggtagtgt ttgttataga
     1561 aaacagataa aaaggtgttg gtagtcagat aaaaaggtgt tggtagtcag ataaaaaggt
     1621 gttggtagtc agataaaaag gtgttggtac cctctgctat cccttagagc cccatagcac
     1681 acagcgcgcc taaaaggttt ttaaaaatat atataaaata tatataaaat aaacgcgtac
     1741 gcgcgcgagg aaatttcccg cctaaaattc ttctcttttt tctacaaaca ggttcgcgat
     1801 atttacaaaa ctgtcattaa aaaatcaaaa gacaaaaaaa gagtgagcga aaatcacgct
     1861 cacattttac ttgcagcgtt cacttcgttc actcgctaaa accaaagtca aaaccattga
     1921 taaggtactc tttggatagg ctctatttgc ttatctaagg ctctctaaga catttttagt
     1981 agttttttat aatcttgtta ggctggcgcc tttcaggcgg tcagggcaag ccctaacaac
     2041 ccgaaaacct ttttcaaccc tgaattacat attgtttatg aaatcttttt tttgacacaa
     2101 ttttatggtt agtagcatct aaaaaatcgc ctatatcgcc tacagaatac attgcataac
     2161 cagcaaaacc accaccaaca gaaccaacca ttccaaattt tatatttgtt atcactgaag
     2221 gttctaacgc actcattaaa acgactagtc tattatcagt agtccatctt agataatttt
     2281 tagcgaaagc ttgtttgacc cattctggct gttcgttagt tttactaatt tcccacacat
     2341 cagctaactt ttttttatat aaagttttca tagtaaacca ccagcgcttt cttattagtt
     2401 acaagagtta ggcgtgccac cttgtccgta aggaccacaa ttacgagctt taaaatttgc
     2461 ataatctgaa ccatttaact gttcagcagt acgagtagga gctaatcctc ctaaaaggcc
     2521 tcctaaaaag tcgccccatc cgccgccaat ggtttgtttt aattctttct ctgacaattc
     2581 tttatacttg ttcgttgaat ttaccgcctt tctttttgta tgaatttttt gattcatttc
     2641 aagattatca tgtgaaaatt agagtcgtca aaagatatct ataggtgaca cgagttatta
     2701 tacttactaa aacaattatc gggataagcc tcggtttttt gacagtcctt ttgaatggtt
     2761 tttactttct tgccaaacct ttctatccgt tgattgttca aacgtaaaag ccttattctc
     2821 taggacttgt tcttcagaca gttgcaaagt atttgttcga gaacggccat ttgccatata
     2881 ctcgccgaca aactcatagc accctgtttc tctgttgtaa aacggggctt ttaagggata
     2941 gaaatgtggg ttcaaggaca ttgttacccc ttttgacccc gtatctggtg gtaaaaacaa
     3001 tgtccctttt tcgtcaatga ggtaagttct atccttggaa tatcgctcta gggatttttt
     3061 taatttaggc gcgttctgaa ggtacttcac tccttgctga atggtaaata aaatggtttc
     3121 tttggcctgt cctagtcttt catgaaggta agtagttaac ccgtcaaact ttaattttat
     3181 ttcttgatat ttttcgttta gttcagaaaa atcgtaggtt aagtcagtaa ttttatcttt
     3241 taaccgctta ttctctgttt ctaacacaga aactgtttgt gttgttctat caagttcttg
     3301 tttgatttgt tcgctctggt catagtttag ttgtttttct tcaaacgctg ttaattgttc
     3361 ttttttcagg gttaattcat aggctaagtc cgttatttgt aattcttgtt catgatttac
     3421 ttgtgtcaat tctttatttt tctgcgccaa ggtattgttt tcgattttaa ggtgcttatt
     3481 ttgcgttcta agcgtttcaa aatctttcgc aatacttttt attgccgaaa ggtcagaagc
     3541 gcttagaacg tattttaaac cgttaaaact aaatttcttt tctttgatat ttgctaaatc
     3601 aggtaaaacg gttgtttgta gagagttaat gatttcgttt cgcttcgttt ccagttggtt
     3661 cattgcttgg actttgccct ttacttcttc gttcttctct tcaagaaggc ggtcaatacg
     3721 gaagattgcc tgttcataag aatggagttc tctctctttt tctgccagaa tttgttcctg
     3781 attagcgagt tcctgcttag aaacttgtag ctccttttga acgtctttgt attctttgac
     3841 agataaatgc ttttgcggac tcccacggag tccacgctca atagcatagc ctcgttcttg
     3901 caaataggtt ggcaattctt cttgaatctc taacaaattt tgccgactaa acacctgttt
     3961 actgctcaat cgcccttcgt acataggcac aattcctaag tgcatatggg gcgtggtttc
     4021 gtctaaatgt accgtggcat aggcgacatt ttgtcgaccg cagcgatcag agaaatatgc
     4081 gacaacatcg gtaaaaaatg cttgtgtgtc tgtattctct tgaaaaaagg cggtatcact
     4141 agtgattatc cattcgttca caaggactgc gtcttttcta accgctcgtt tactgattcg
     4201 ttggctctca atgatttgtt tgattttctc ccgataattg attgaaccag gattgactaa
     4261 gtcataattc aagtgagatt tctcaatatc aatatcagga ttggaatgat tattggtttc
     4321 tcgttgatta tgtcgttgga taccacttaa atttccgtct ttcatttttt ccatacgtgc
     4381 aacaattttt gacatgagcg ctcctccccc ttttttttct tgtgtcgttc ggtgtctttg
     4441 gatttcggca gtacttccaa gtaaagtata acgcactata ctttacttcg taaaggtgcg
     4501 ctctgccgag ggctgtcggc tagtactcga aaggtcagcc tttaggcttc ccaatctcgc
     4561 acttagccga ttgcctacgg tcaaactttc catttctgct aaggcagaaa ctccaaattt
     4621 ccctccgtca gaaaaccgtt taaataacga ctgcctgaaa ttagtgaacg tgctagcttt
     4681 ctgctagccg gtgctagtaa tgtgctaatt tggtgctaaa aacctgctag tcactgctag
     4741 gaatctgcta gtttgggtgc tagaaacctg ctattgagtg ctaaaaatgt gctagtttac
     4801 ctgaaagtac ctgctagtaa agtgctagcg aagaacgaaa gtgtacc
//
