LOCUS       NZ_CP138638            21899 bp    DNA     circular CON 21-NOV-2023
DEFINITION  Enterococcus faecalis strain ES-3-1 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP138638
VERSION     NZ_CP138638.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38203339
            Assembly: GCF_033811895.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21899)
  AUTHORS   Yoon,J.W.
  TITLE     Complete genome sequences of Enterococcus faecalis isolated from
            pigs in South Korea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21899)
  AUTHORS   Yoon,J.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2023) Department of Veterinary Medicine, Kangwon
            National University, 1 Gangwondaehakgil, Chuncheon, Gangwon 24341,
            South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: De novo assembly using Unicycler v. 5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 559x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/21/2023 09:44:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,883
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 18 of 49
            Pseudo Genes (incomplete)         :: 32 of 49
            Pseudo Genes (internal stop)      :: 8 of 49
            Pseudo Genes (multiple problems)  :: 6 of 49
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138638.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21899
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="ES-3-1"
                     /isolation_source="Pig rectum feces"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed1"
                     /geo_loc_name="South Korea:Gyeonggi-do, Anseong-si"
                     /collection_date="2021-07-05"
                     /collected_by="Nguyen Thi Mai Tho, Jun Bong Lee"
     gene            100..1734
                     /locus_tag="SHT66_RS14255"
                     /old_locus_tag="SHT66_14255"
     CDS             100..1734
                     /locus_tag="SHT66_RS14255"
                     /old_locus_tag="SHT66_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein PoxtA2"
                     /protein_id="WP_209691075.1"
                     /translation="MKGKNMNLAFGLEEIYEDAEFQIGDLDKVGIVGVNGAGKTTLFRL
                     LLGELELDNGSLTSGNARIGYLPQEIVLEDEDITVWDFLFEGRPIKKYEQELEEIYKKL
                     ETAVNAEQEALLARMGTLQERLEYFDFYEAETILLEFADKMSIDAELYHRPMRELSGGQ
                     KSKMAFARLLYSKPEILLLDEPTNHLDVSTKDFVIKYLKNYRGSVLIISHDIDFLNRII
                     NKIMYINKATHKISVYDGDYYIYKKKYAEEQRIREMAIVQQEKEIKELSDFVQKAKQAS
                     QTNHHLKRMGQERALRLDKKRGELQKRNRLYKRVKMDIRPKREGAQVPLEVENITFHYS
                     GYPTLYQNLSFQINGRERFLVVGENGVGKSTLLKLMMGILSPDEGCIRFNQKTDIAYYA
                     QELEQLDENKTVIDNVESEGYTPWQIRAVLSNFLFYDDDVNKKVSVLSPGEKARVALCK
                     ILLQKANLLILDEPTNHLDPETQKIIGGNFNLFEGTIIAVSHNPSFVEQIGISRMLILP
                     SGRIEPYSRELLEYYYEINTPEEEQKY"
                     /name="ABC-F type ribosomal protection protein PoxtA2"
     ARG             1754..2934
                     /name="cfr(D)"
                     /gene="cfr(D)"
                     /gene_name="Cfr 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="lincosamide antibiotic; streptogramin
                     antibiotic; oxazolidinone antibiotic; glycopeptide
                     antibiotic; phenicol antibiotic"
                     /identity="99.72"
                     /coverage="110.08"
     ARG             1862..2931
                     /name="cfr(D)"
                     /gene="cfr(D)"
                     /gene_name="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(D)"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL/LINCOSAMIDE/OXAZOLIDINONE/PLEUROMUTIL
                     IN/STREPTOGRAMIN"
                     /identity="99.72"
                     /coverage="100.0"
     gene            complement(3162..3398)
                     /locus_tag="SHT66_RS14265"
                     /old_locus_tag="SHT66_14265"
     CDS             complement(3162..3398)
                     /locus_tag="SHT66_RS14265"
                     /old_locus_tag="SHT66_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012798.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_224994002.1"
                     /translation="MPNRELKKIVDKYKMTEISVHGFRHSHASLLFEAGLDVKSVQDRL
                     GHSDVQTTLQIYTHVTEKMKNNSGEKFQKYVNF"
                     /name="tyrosine-type recombinase/integrase"
     gene            3772..4044
                     /locus_tag="SHT66_RS14270"
                     /old_locus_tag="SHT66_14270"
     CDS             3772..4044
                     /locus_tag="SHT66_RS14270"
                     /old_locus_tag="SHT66_14270"
                     /function="plasmid copy number control"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_011266100.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTIK
                     TLEQIAKLTNSTLVVDLIPNEPTEPESEQVTLDLEMEEEKSNDFV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            4428..5918
                     /locus_tag="SHT66_RS14275"
                     /old_locus_tag="SHT66_14275"
     CDS             4428..5918
                     /locus_tag="SHT66_RS14275"
                     /old_locus_tag="SHT66_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_256969073.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSIITKPGKSKGAIFAYRSK
                     SSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQINTF
                     FIDFDIHTEKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSEFKS
                     VKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQDWSFK
                     QTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLVGRNSVMFTLSLAYF
                     SSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILCKAWVSS
                     DLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVTTKKEIRE
                     ALGIPERTLDKLLKVLKANQEIFFKIKSGRNGGIQLASVKSLLLSIIKLKKEERESYIK
                     ALTASFNLERTFIQETLNKLAERPKTDTQLDLFSYDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            6266..6436
                     /locus_tag="SHT66_RS14280"
                     /old_locus_tag="SHT66_14280"
     CDS             6266..6436
                     /locus_tag="SHT66_RS14280"
                     /old_locus_tag="SHT66_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164742224.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEQVA
                     LADWLLQPREF"
                     /name="hypothetical protein"
     gene            6450..7067
                     /locus_tag="SHT66_RS14285"
                     /old_locus_tag="SHT66_14285"
     CDS             6450..7067
                     /locus_tag="SHT66_RS14285"
                     /old_locus_tag="SHT66_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_128712832.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQLQ
                     AMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENLRR
                     LINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAFDLF
                     LNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
                     /name="recombinase family protein"
     gene            7067..9211
                     /locus_tag="SHT66_RS14290"
                     /old_locus_tag="SHT66_14290"
     CDS             7067..9211
                     /locus_tag="SHT66_RS14290"
                     /old_locus_tag="SHT66_14290"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_256969070.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEITDPLFTDETFITF
                     GFGHLVELAEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVAKLLKQANTII
                     VATDSDREGENIAWSIIHKANAFSKDKTFKRLWINSLEKDVIRSGFQNLQPGMNYYPFY
                     QEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIYQRQEAIENFKK
                     EPFFEVEASIKVNQGSFKGVLSPTQRFKTQEELLAFVSSKQAKIGNQEGRIADVQTKEK
                     KTNSPSLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITENEFAYL
                     KANFGKYSGFLGLDLEMVQTEPRKRYVDGSKVQEHHAIIPTKQVPTESALAKMDDLQRK
                     IYALVVKTTVAMFLPDYLYEETKIQTKVANLLFQSIGKTPKQEGWKILFKQQTKEEKED
                     VQTLPLVIIGEHAEVDVKSAEKETQPPKAFTEGTLLTAMKTANKTVDDEEAIKILQEVE
                     GIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKLLCQAVESQHLLTSAEMTAKWETY
                     LKKIGKREGNQENFITNIKKFIVHLLEAVPNDIEKLNFSDYQEQKEKEAEKSIVGKCPK
                     CGNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGKETLVKGIKTKDKKS
                     YNAVVKIGEKGYIDFISFSK"
                     /name="type IA DNA topoisomerase"
     gene            9300..10196
                     /locus_tag="SHT66_RS14295"
                     /old_locus_tag="SHT66_14295"
     CDS             9300..10196
                     /locus_tag="SHT66_RS14295"
                     /old_locus_tag="SHT66_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_002387620.1"
                     /translation="MIQYYYTKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYFK
                     GGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGN
                     LASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTV
                     PTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLV
                     DTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYEN
                     VFFEMLERIIQLENEKE"
                     /name="ParA family protein"
     gene            10288..10503
                     /locus_tag="SHT66_RS14300"
                     /old_locus_tag="SHT66_14300"
     CDS             10288..10503
                     /locus_tag="SHT66_RS14300"
                     /old_locus_tag="SHT66_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="WP_001835296.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETA
                     KNGGNVKEVMDQALEEYIRKYLPDKL"
                     /name="peptide-binding protein"
     gene            10520..10792
                     /locus_tag="SHT66_RS14305"
                     /old_locus_tag="SHT66_14305"
     CDS             10520..10792
                     /locus_tag="SHT66_RS14305"
                     /old_locus_tag="SHT66_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="WP_000301765.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVN
                     LLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
                     /name="antitoxin"
     gene            10794..11657
                     /locus_tag="SHT66_RS14310"
                     /old_locus_tag="SHT66_14310"
     CDS             10794..11657
                     /locus_tag="SHT66_RS14310"
                     /old_locus_tag="SHT66_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="WP_256969069.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSG
                     KTSLRSAIFEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAIIS
                     RLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVIAVPKINSYLGTIERYETMY
                     ADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISP
                     KETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPTPPIPKI
                     PKLPGL"
                     /name="zeta toxin family protein"
     gene            complement(11721..12405)
                     /locus_tag="SHT66_RS14315"
                     /old_locus_tag="SHT66_14315"
     CDS             complement(join(11721..12371,12373..12405))
                     /locus_tag="SHT66_RS14315"
                     /old_locus_tag="SHT66_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_256969067.1"
                     /translation="MNHFKGKQFKKDVIIVAVSYYLRYNLSYREVQELLYDRGINVCHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGGFYQGIEHRTIKYL
                     NNLIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVL
                     LGIPA"
                     /name="IS6 family transposase"
     gene            12654..13886
                     /locus_tag="SHT66_RS14320"
                     /old_locus_tag="SHT66_14320"
     CDS             12654..13886
                     /locus_tag="SHT66_RS14320"
                     /old_locus_tag="SHT66_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543631.1"
                     /GO_function="GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity [Evidence
                     IEA]"
                     /GO_process="GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="WP_256969066.1"
                     /translation="MLLETYLKNNNTNIRQMHIVSGIPETTVRGINKRDFSKWSVTHFD
                     AIARTVGKERHVVMQELEALKEQADNFAPLGRYNLENRRYIGNKNKLLNWISDLIDAHT
                     EGNSFFDVFAGTGVVSKEMLGKYDSFIMNDFLFSNNIIYKAFFSNDSFDSNKLLSRQEE
                     FNRIITNKEDDDYFSDNFGGKFFSVHDAKVIGEIRTRIEKDKSLTEKERAILIASLIYS
                     SDKIANTVGHYDAYRKKVQIADRFKFELVNPLDTSSKDIQIFREDSNELVRKISGDVAF
                     IDPPYNSRQYSRFYHVLEGIAKWDKPELGGVAMKPPAENMSDYSRSSAPKVFDDLIQNL
                     DVKYIVVTYNNTYKSKSSSSKNKITHDEILESLNKVGKTKSFEMPFQFFNAGKTDLKDH
                     KEFVFITEVGK"
                     /name="DNA adenine methylase"
     gene            13886..14860
                     /locus_tag="SHT66_RS14325"
                     /old_locus_tag="SHT66_14325"
     CDS             13886..14860
                     /locus_tag="SHT66_RS14325"
                     /old_locus_tag="SHT66_14325"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543632.1"
                     /GO_function="GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="WP_256969065.1"
                     /translation="MQTTLFPTETLEVVAPMKKKGKAIRSPLFYVGDKYKLMPQLKELF
                     PKNINNYYDVFSGGGSASINVIADKIIMNDVDEKVVELHRFLQEQSSDIELFIENMYEL
                     IREYGLSLSELGKNSEIEELKKEFKKTYFAKYNKQAYLKLRDDYNKNQSNTMLLYLLLI
                     YGFNHMIRFNGQGQFNLPVGNVDWNKNVSTALRNYSQWYNNNNIEISSGMDFEDFVNFY
                     DFKNGDFLYFDPPYLITFSDYNKLWGEDEERRLYKLLDELDSKGIKWGLSNMYNHKGKT
                     NEILLEWGKKYNEYDIKSNYISRFDNTIKKDSKEIYITNYTYE"
                     /name="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
     gene            14952..16724
                     /locus_tag="SHT66_RS14330"
                     /old_locus_tag="SHT66_14330"
     CDS             14952..16724
                     /locus_tag="SHT66_RS14330"
                     /old_locus_tag="SHT66_14330"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="WP_256969064.1"
                     /translation="MSRVRRRKPITFDTTLRNPGRIPQFISVLSEFEGKVIDDKLALEL
                     EGEIIRQKIFEPTKATLGTYMKEYNKKFHFVAADQSEEAASKVEEIYIQWEKGSPGSVP
                     LEKITYLLENTITAHKDRGWKGGWESRIHTQFNFLNELGFVRVVKGEKIIISKNGKLMI
                     HEYSNGYPIKDTYDESFEQSAFLNAFSKYQVNNPYRSNTIKVNFFPLVLNVINYLDEKY
                     QRPGISKQDLPFIITWGNDDYQTLAEYIYQFRVKFGYNTSNELVYEYAMNLLDDSTPND
                     TIAPATVDFIEQKKRDYKFDKIILETPDEVIRKLRLTMLISLRGAGRFIDINKNELEKI
                     KHVLANYSSNIDFDDDVQKYLEYMGDIDNSLMFVSDAQESQEAKYAKEVAIEKWAKEYD
                     WTFLKEEMVNSVEKRPSSHLILKYVKETARLEFLSAIVIKKALPKLKVVANYKADDQGI
                     PFNTASGGKRNLIGADIDVFEDNIHAILEPTISKQRSFQVEHELPSIRNHVLGTANKDV
                     EEGNNFKEWFSLFIASNITRDVGDQVALIKQTNGVEIYPWDIKDFVEYSERVSTIRDYK
                     KIRNYVKPQSMPVF"
                     /name="AlwI family type II restriction endonuclease"
     gene            <16782..17189
                     /locus_tag="SHT66_RS14335"
                     /old_locus_tag="SHT66_14335"
                     /pseudo=""
     CDS             <16782..17189
                     /locus_tag="SHT66_RS14335"
                     /old_locus_tag="SHT66_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /name="toxin zeta"
     gene            17629..17823
                     /locus_tag="SHT66_RS14340"
                     /old_locus_tag="SHT66_14340"
     CDS             17629..17823
                     /locus_tag="SHT66_RS14340"
                     /old_locus_tag="SHT66_14340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319464680.1"
                     /translation="MEKTKKLQLEDFTENEFFGTQEQQYLKAQVREELKEQGFIIDSSF
                     EGDFKTWIGGFCCKVLNKE"
                     /name="hypothetical protein"
     gene            complement(17846..18526)
                     /locus_tag="SHT66_RS14345"
                     /old_locus_tag="SHT66_14345"
     CDS             complement(17846..18526)
                     /locus_tag="SHT66_RS14345"
                     /old_locus_tag="SHT66_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_013646119.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(18658..>18786)
                     /locus_tag="SHT66_RS14350"
                     /old_locus_tag="SHT66_14350"
                     /pseudo=""
     CDS             complement(18658..>18786)
                     /locus_tag="SHT66_RS14350"
                     /old_locus_tag="SHT66_14350"
                     /inference="COORDINATES: protein motif:HMM:NF015935.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /name="JAB domain-containing protein"
     gene            19237..19380
                     /locus_tag="SHT66_RS14355"
                     /old_locus_tag="SHT66_14355"
     CDS             19237..19380
                     /locus_tag="SHT66_RS14355"
                     /old_locus_tag="SHT66_14355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002360221.1"
                     /translation="MGIDFINVEFRKHVKVSGDLGTTGSVCPFSQKQKMETLPENFLPP
                     FI"
                     /name="hypothetical protein"
     gene            19634..21061
                     /gene="fexA"
                     /locus_tag="SHT66_RS14360"
                     /old_locus_tag="SHT66_14360"
     ARG             19635..21058
                     /name="fexA"
                     /gene="fexA"
                     /gene_name="chloramphenicol/florfenicol efflux MFS
                     transporter FexA"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="98.95"
                     /coverage="100.0"
     gene            complement(21145..21825)
                     /locus_tag="SHT66_RS14365"
                     /old_locus_tag="SHT66_14365"
     CDS             complement(21145..21825)
                     /locus_tag="SHT66_RS14365"
                     /old_locus_tag="SHT66_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_013646119.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     ARG             101..1731
                     /name="poxtA2"
                     /gene="poxtA2"
                     /gene_name="ABC-F type ribosomal protection protein PoxtA2"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL/OXAZOLIDINONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             116..1734
                     /name="poxtA"
                     /gene="poxtA"
                     /gene_name="Miscellaneous ABC-F subfamily ATP-binding
                     cassette ribosomal protection proteins"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic; oxazolidinone
                     antibiotic; phenicol antibiotic"
                     /identity="100.0"
                     /coverage="99.45"
     Typing          4429..5918
                     /name="Inc18"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.732"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaaaatata tccccgcaca ggagataatc aagcaaggaa aactgtaagc cataaaatga
       61 tggcttgcag tatgtatgac gatggaggaa tgatacaaga tgaaaggtaa aaatatgaat
      121 ttagccttcg ggttggaaga aatttatgag gatgctgagt ttcaaatcgg agatttggat
      181 aaggtcggta ttgtcggcgt gaacggagcc ggaaagacca ccttgttccg cctgctgttg
      241 ggagaacttg aacttgataa tggttcactg accagtggaa atgcccgtat tggttatctc
      301 ccacaggaaa ttgtcttgga agatgaggat attaccgttt gggatttcct ttttgaggga
      361 cgtccgatta aaaagtatga gcaggaattg gaagaaatct ataaaaagct tgaaaccgca
      421 gtcaatgcag agcaggaagc actgcttgcc cgaatgggaa cattgcaaga acgcttggag
      481 tatttcgact tctatgaggc agaaacaatt ctgttggagt ttgcagataa aatgagcatt
      541 gatgcagaat tatatcatcg tccgatgaga gagctttcag gcggacaaaa atccaaaatg
      601 gcatttgcca gactactata ttcaaaaccg gaaattctat tgttggatga gcctaccaac
      661 catttagatg tcagcacaaa ggattttgtt ataaaatact taaagaatta taggggttcg
      721 gtactgatta tcagccatga tattgatttt ctaaatcgga ttatcaacaa aattatgtac
      781 atcaacaaag ctacccataa aatatctgtt tatgatggag actactacat ctacaagaaa
      841 aagtatgcag aggaacagcg gattcgtgaa atggcgattg tacagcagga aaaagaaata
      901 aaggagcttt ccgattttgt acaaaaagca aaacaagcca gtcagaccaa tcatcacctc
      961 aaacgaatgg gtcaagagcg agccttgcgg cttgataaaa agcgtggaga gctgcaaaag
     1021 agaaatcgac tgtacaagcg tgtgaagatg gatattcgcc ccaagcgtga aggggcacaa
     1081 gttcccttag aggtggaaaa tatcaccttc cactattcgg ggtatcccac cctttatcag
     1141 aacctttcct ttcagattaa cggaagagaa cgatttcttg tggtgggtga aaacggtgtc
     1201 ggtaaatcca ccttattgaa attgatgatg ggtattctca gtccagatga aggatgcatt
     1261 cgctttaacc agaaaactga tattgcatat tatgcacagg aactcgaaca gcttgatgaa
     1321 aacaaaacgg tcattgacaa tgtggagtct gaaggatata caccgtggca aatcagagcc
     1381 gtactgagca acttcctgtt ttatgatgac gatgtaaaca agaaagtatc tgtgctgtcc
     1441 cctggagaaa aagcaagggt tgccctttgc aaaatcctat tacagaaagc caatcttttg
     1501 atactggacg agccgaccaa ccaccttgac ccagaaacgc agaaaatcat tggcggcaac
     1561 ttcaatttgt ttgagggaac cattattgcc gttagccata acccatcctt tgtggaacaa
     1621 atcggaatta gccgtatgct tattttgccc agcggtcgaa ttgaacccta ttcccgtgag
     1681 ctgcttgagt attattatga aatcaacact cccgaagaag aacagaaata ctaatgaaaa
     1741 gcaacgattt acttggttct gttgcaaagt tttatattta tgaatgaaaa ggcgtatgaa
     1801 atattgtcca tactccttct cattactcat aaaaaaagag aacgtgagag ggtttataat
     1861 atgttacaaa aacaattaac taaatatcaa caaattgaaa aagttttaaa agagtgtcaa
     1921 caaccaaact accgaatgaa gcaaattctt cattgtattt ttaaagagaa aaaaacagat
     1981 ttcaatgaga tgtctgtatt acctaaaaat ttacgagata ctttaacaac agaaatagga
     2041 acatcacttc taacagttga gccccttatt gaacagaagt cgcaacaagt gaggaaagta
     2101 ctctttaatc tttctggaga taatcgcatt gaaacagtca acatgaccta tcaagctggc
     2161 tgggaatctt tttgtatttc atctcaatgt ggctgcaatc taggttgtca attttgcgct
     2221 actggaaaaa ttggcttaag aaaaaattta acggctgagg aaataacgga tcaagttctc
     2281 tactttcttt taaaaggaca ccaaattgat agtatttctt ttatgggtat gggagaacca
     2341 cttgctaacc ctcatttttt tgaggcttta gatattttta tgaatcctga tatgtttcaa
     2401 ctgagcccta ggcgtttatc tgtttcaacg attggtgtga ttcctaaaat aaaacgacta
     2461 acagctgaat atccacaagt tcatttaact ttttctcttc actcaccttt tgatgaagag
     2521 agaagtcagt taatgccgat taataaaagt tttccccttt taaaagtgat ggatacttta
     2581 gatgaacata taaaagtaac ttcaaaaaaa gtttacattg cttatatttt actacctgat
     2641 gtgaacgatt ctttgactca tgcagttgct ttagcagatt tacttcgctc tcggtataaa
     2701 aaaggaaagc tgtatcatgt caatctcatc aggtacaatc ctacttttga tgcaccaaga
     2761 acgttcaagc aagtagatga aaagcaagtt aaactgttct atcaaacctt aatatctaaa
     2821 ggcataaaag tcactattag aagtcagttt ggaattgaaa ttgatgctgc ctgtggccaa
     2881 ttatatggaa attatgagat taaaaacaaa aaaacaatcc aagtaaatga ctagattgtt
     2941 tctcatttaa ttaaattagt tggataacta aaaagtaccg aataatccat aattagatta
     3001 ttcggtactt ttttaaatta ttcccactct atagttgcac agtaatttta ccatgatatt
     3061 tttatagagg caaatggcta tttatcaatg tttttatgtt gttttatcgt tatttaaacc
     3121 atgctatgaa ttggaattaa tgccatagtt aatgccattt gctaaaaatt gacatacttt
     3181 tggaattttt cacctgaatt atttttcatt ttttctgtta catgagtata tatctgtaaa
     3241 gtagtctgaa catcagaatg tcctaatcta tcctgaacag atttaacatc aagaccagct
     3301 tcaaataata aactagcatg agaatgacga aaaccatgta ctgatatttc agtcatctta
     3361 tatttatcaa cgattttttt tagttctcta tttggtatag gggtcccgag cgcttagtgg
     3421 gaatttgtat cgataaggaa tagatttaaa aatttcgctg ttattttgta caataaggat
     3481 aaatttgaat ggtaccataa acgaccgttt atggtaccat ttcattttcc tgctttttct
     3541 aaatggggtc ccgagcgctt agaatcgctt taggaaacac gatccagtcc aataatcgtc
     3601 gataaaaact tttgaaaaag gttggtgaaa ttacctactt ttggaataat cacaaatcac
     3661 aagtgattaa tcacaaatca caagtgatta atcacttgtt tattaagata ttaaaagcta
     3721 taatttaaaa taaagcgtga attttattac acaaaaagag gggggagaaa cttggaacta
     3781 gcatttagag aaagcttaaa aaagatgaga ggtaccaaat caaaagaaaa attctcccaa
     3841 gaattagaaa tgagtagatc aaattattca cgaatagaat caggaaaatc agatccaacc
     3901 ataaaaacac tagaacaaat tgcaaagtta actaactcaa cgctagtagt ggatttaatc
     3961 ccaaatgagc caacagaacc agaatcagaa caagtaacat tggatttaga aatggaagaa
     4021 gaaaaaagca atgatttcgt gtaaaaaacg cacgaaatca ttgcttattt ttttaaaaag
     4081 cgatatacta gatataacga aacaacgaac tgaataaaga atacaaaaaa agagccacga
     4141 ccaacttatt ttccgccaag aaaataagtt gcgagcctta attgattacc accaatcaat
     4201 taaagaagtc gagacccaaa atttggtaaa gtatttaatt actttattaa tcagatactt
     4261 aaatatctgt aaacccatta tatcgggttt ttgaggggat ttcaagtctt taagaagata
     4321 ccaggcaatc aattaagaaa aacttagttg attgcctttt ttgttgtgat tcaactttga
     4381 tcgtagcttc taactaatta attttcgtaa gaaaggagaa cagctgaatg aatatccctt
     4441 ttgttgtaga aactgtgctt catgacggct tgttaaagta caaatttaaa aatagtaaaa
     4501 ttcgctcaat cattaccaag ccaggtaaaa gcaaaggggc tatttttgcg tatcgctcaa
     4561 aatcaagcat gattggcgga cgtggcgttg ttctgacttc cgaagaagcg attcacgaaa
     4621 atcaagatac atttacacat tggacaccaa acgtttatcg ttatggtacg tatgcagacg
     4681 aaaaccgttc atacactaaa ggacattctg aaaacaattt aagacaaatc aataccttct
     4741 ttattgattt tgatattcac acggaaaaag aaactatttc agcaagcgat attttaacaa
     4801 cagctattga tttaggtttt atgcctacgt taattatcaa atctgataaa ggttatcaag
     4861 catattttgt tttagaaacg ccagtctatg tgacttcaaa atcagaattt aaatctgtca
     4921 aagcagccaa aataatctcg caaaatatcc gagaatattt tggaaagtct ttgccagttg
     4981 atctaacgtg caatcatttt gggattgctc gtataccaag aacggacaat gtagaatttt
     5041 ttgatcccaa ttaccgttat tctttcaaag aatggcaaga ttggtctttc aaacaaacag
     5101 ataataaggg ctttactcgt tcaagtctaa cggttttaag cggtacagaa ggcaaaaaac
     5161 aagtagatga accctggttt aatctcttat tgcacgaaac gaaattttca ggagaaaagg
     5221 gtttagtagg gcgcaatagc gttatgttta ccctctcttt agcctacttt agttcaggct
     5281 attcaatcga aacgtgcgaa tataatatgt ttgagtttaa taatcgatta gatcaaccct
     5341 tagaagaaaa agaagtaatc aaaattgtta gaagtgccta ttcagaaaac tatcaagggg
     5401 ctaataggga atacattacc attctttgca aagcttgggt atcaagtgat ttaaccagta
     5461 aagatttatt tgtccgtcaa gggtggttta aattcaagaa aaaaagaagc gaacgtcaac
     5521 gtgttcattt gtcagaatgg aaagaagatt taatggctta tattagcgaa aaaagcgatg
     5581 tatacaagcc ttatttagtg acgactaaaa aagagattag agaagcgcta ggcattcctg
     5641 aacggacatt agataaattg ctgaaggtac tgaaggcgaa ccaggaaatt ttctttaaga
     5701 ttaaatcagg aagaaatggt ggtattcaac ttgctagtgt taaatcattg ttgctatcga
     5761 tcattaaatt aaaaaaagaa gaacgagaaa gctatataaa ggcgctgaca gcttcgttta
     5821 atttagaacg tacatttatt caagaaactc taaacaaatt ggcagaacgc cccaaaacgg
     5881 acacacaact cgatttgttt agctacgata caggctgaaa ataaaacccg cactatgcca
     5941 ttacatttat atctatgata cgtgtttgtt ttttctttgc tgtttagtga atgattagca
     6001 gaaatataga gagtaagatt ttaattaatt attaggggga gaaggagaga gtagcccgaa
     6061 aacttttagt tggcttggac tgaacgaagt gagggaaagg ctactaaaac gtcgaggggc
     6121 agtgagagcg aagcgaacac ttgatttttt aagtttctat cttttatagg tcattagagt
     6181 atacttattt gtcctaatat ggttttagca gtataataga tttattgaat aggtcattta
     6241 agttgggcat aataggagga gtaaaatgaa aaaatttatt tatcgagttt tagaaaatga
     6301 cgaagtggtg gctattttta atgagcaaca atatgcgcaa gattttatcg cttatgaaaa
     6361 gacaatttct gataagcaat ttgaaattga acaagtagca cttgcagatt ggttattgca
     6421 accgagagaa ttttagaggt tggttgaaaa tggctaaaat tggttatgca cgtgtcagta
     6481 gcaaagaaca gaacttagat agacaactga aagcgttaga gggcgtttct aaggtctttt
     6541 cagacaaatt aagcggtcaa tcggtcgaac gcccacaatt acaagctatg cttaactata
     6601 ttcgtgaagg ggatatagtt gttgttactg aattagatcg attaggacga aataataaag
     6661 aattaacaga attgatgaat caaattcaaa ttaagggggc aaccctggaa gtcttaaatt
     6721 taccctcaat gaatggtatt gaagatgaaa atttaagacg gctgattaat aatttagtga
     6781 ttgaattgta taagtaccaa gcggaatctg aacgcaaacg aattaaagaa cgccaagccc
     6841 aaggaattga aattgctaag aaaaaaggaa aattcaaagg gcgacaacct aaattcaaag
     6901 aaaatgatcc acgtttacaa cacgctttcg atttgttttt gaatggttta tccgataaag
     6961 aagttgaaga acaaactgga atcaatcgcc gaacgtttag aaggtatcga gcaagatata
     7021 acgtgaccgt agatcaaaga aaaaacaatg aaaagaggga tagttaatga gtacggttat
     7081 tttagctgaa aaaccgagcc aggcattagc ctacgcaagt gctttaaaac aaagcaccaa
     7141 aaaagacggt tattttgaga tcacagaccc actatttaca gatgaaacgt ttatcacctt
     7201 tggttttggg catttagtgg aattagcaga accaggtcat tatgacgaaa agtggcaaaa
     7261 ttggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
     7321 taagggaaag cagtttaaga tcgttgccaa acttttaaaa caagcaaata caattattgt
     7381 tgcaacagat agcgacagag aaggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa
     7441 tgccttttca aaagataaaa catttaaaag actatggatc aatagcttag aaaaagatgt
     7501 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
     7561 agcgcaaaca cgccaaattg ctgattggtt gatcggcatg aacgcaagcc ctttatatac
     7621 cttgaattta caacaaaaag gcgtacaagg tacattttct ttaggacgtg tacaaacacc
     7681 gactttatat ctcatttatc aacgccagga agccatagag aattttaaaa aagaaccttt
     7741 ttttgaggtg gaagctagta taaaagtaaa ccaagggtca tttaagggcg ttctaagccc
     7801 cacacagcgt tttaaaaccc aagaggagct tttagctttt gtttcttcta aacaagctaa
     7861 aataggcaat caagagggca gaatcgctga cgtgcaaacc aaagagaaaa aaacgaatag
     7921 tccgagtctg ttttctttaa gtagtttgca atcaaaagtc aatcagcttt ataaagcgac
     7981 agcgagccaa actttaaaag ctatgcaagg actgtatgaa gcaaaattat tgagttatcc
     8041 aagaacagat accccattta ttacagagaa cgaatttgct tatttaaaag cgaattttgg
     8101 caaatatagc ggttttttag gacttgacct tgaaatggtt caaacagagc ctagaaagcg
     8161 ttatgtggac ggtagtaagg tacaggaaca ccacgccatt atcccaacaa aacaagtacc
     8221 taccgaatct gcattagcga aaatggacga tttacaacga aaaatttatg ctttagtcgt
     8281 taaaacgacc gttgccatgt ttttacctga ttacttgtat gaagaaacca agatacaaac
     8341 gaaagtagct aatttgctgt ttcaatcgat cggaaagaca ccaaaacaag aaggttggaa
     8401 aattcttttc aaacaacaaa ccaaagaaga aaaagaggac gttcaaacgt tacccttggt
     8461 tatcattggc gaacatgccg aggttgacgt taagagtgcc gaaaaagaaa cacaaccacc
     8521 gaaagctttt acagagggta cattattaac tgctatgaaa accgctaata aaacggttga
     8581 tgatgaagaa gcaatcaaga ttttacaaga agtcgagggg atcggaacag aagcgacaag
     8641 agcaagcatt attgaagcct tgaaacaaaa agaatatatc caagtgatta agaataagct
     8701 tgttgtaact gaaaaaggaa aattattgtg ccaggcagtt gaaagtcagc accttttaac
     8761 gagtgctgaa atgacggcta aatgggaaac gtatttaaaa aaaatcggta aaagagaagg
     8821 caatcaagag aactttatta cgaatatcaa aaaattcatt gttcatttac tggaagctgt
     8881 acctaacgat atagaaaaac taaatttttc tgattaccag gaacagaaag aaaaagaagc
     8941 agaaaaaagt attgtaggaa aatgtcctaa gtgtggcaac aatattgtat taaaaaaatc
     9001 gttttatggt tgttcaaatt atcctgaatg taagtttact ttagctgaac attttagaaa
     9061 gaaaaaactc accaaaacaa atgtaaaaga attactagag ggaaaagaaa ccctggtaaa
     9121 aggaatcaaa acgaaagata aaaagtccta caatgccgtt gtaaaaatcg gagaaaaggg
     9181 atatattgat tttatctctt tttcaaaata agcataaaag ccctttaaac agggctttta
     9241 tatattaatc acaaatcact tatcacaaat cacaaatcac ttgtgatttg tgattcctaa
     9301 tgatacaata ttactataca aaaaaagaat ggggcgtagt tatggagaag gaagaactaa
     9361 aaatacttga agaattaaga cgtattttaa acagtaaaaa tgaagcaatt gttatcttaa
     9421 acaattattt taaaggtggt gttggaaagt caaaattatc gactatgttt gcttatttga
     9481 cagacaaatt taatttaaaa gttttaatga tcgataagga cttacaagca acattgacaa
     9541 aagacttagc aaaaacattt aaggtagaat tgccacgtgt caatttttat gaaggcttga
     9601 aaaatggaaa cttggcttct tctattattc atttgactga taatttagac ttgatccctg
     9661 gcacgtttga tttgatgtta ctgccaaaat taactcgctc atggacgttt gaaaatgaaa
     9721 gtagattgct tgctactctt ttagcacctt taaaaagtga ctatgatctc attattattg
     9781 atactgtacc aacgccaagc gtttatacaa ataatgcaat cgtggcaagt gattacgtta
     9841 tgatcccttt acaagcagaa gaagaaagta caaacaacat tcaaaactat atttcctatt
     9901 tgattgattt acaagaacag tttaaccctg gactagatat gatcggtttt gttccttatt
     9961 tagttgatac ggacagcgca acgataaaat caaacctgga agaactgtac aagcaacata
    10021 aagaagataa cttggttttc caaaatatta tcaagcgaag taataaagta agtacctggt
    10081 ctaaaaacgg aattacagaa cacaaaggct atgacaaaaa agttttatcc atgtatgaga
    10141 acgtattttt tgaaatgctt gagcgaatca ttcaattaga aaacgaaaaa gaatagaatc
    10201 acaaatcaca agtgattaat cacaaatcac ttgtgatttg tgattgttga tgataaaata
    10261 agaataagaa gaaatagaaa gaagtgagtg attgtgggaa atttaggcgc acaaaaagca
    10321 aaacgaaatg atacaccaat cagtgcaaaa aaagatataa tgggagataa gacggttcgt
    10381 gttcgtgctg acttgcacca tatcataaaa atcgaaacag caaagaatgg cggaaacgta
    10441 aaagaagtta tggatcaagc cttagaagaa tatatacgga aatatttacc tgacaaactt
    10501 taaaaggagt gaaaatgaaa tggcagttac atatgaaaaa acatttgaaa ttgaaatcat
    10561 taatgagtta tcggcaagtg tttataatcg agtattaaac tatgttttga accatgaatt
    10621 aaataaaaat gactctcaat tattggaagt caatttatta aaccaattaa agcttgcaaa
    10681 acgtgtaaat ctttttgatt attctttaga agaattacaa gccgttcatg agtattggcg
    10741 gtcaatgaat cgttactcaa aacaagtttt gaataaagag aaagtggctt aatatggcaa
    10801 atatagtcaa ttttactgac aaacaatttg agaatcgctt aaatgataat ttagaagaat
    10861 tgattcaagg aaaaaaagcg gttgaatcgc caaccgcttt tttacttggt gggcaaccag
    10921 gatcagggaa aaccagtttg cgatcagcaa tttttgaaga aacacaagga aatgttgtta
    10981 tcattgataa tgatacgttc aaacaacagc acccaaattt tgacgaacta gtgaaactct
    11041 atgaaaaaga cgtagtaaaa cacgttaccc cttattctaa tcgcatgaca gaagcgatca
    11101 taagccgttt aagcgatcaa ggctataatt tagtgattga aggtacagga cgaacaacag
    11161 acgttcctat tcaaaccgca acaatgctac aagccaaagg ttatgaaaca aaaatgtatg
    11221 tcatagcagt accgaaaatc aactcatatt taggaacaat tgaacggtat gaaactatgt
    11281 atgcagatga tccaatgaca gctagggcaa caccaaaaca agcgcatgat attgttgtca
    11341 aaaacttacc gaccaattta gaaacccttc ataaaacggg cttatttagc gatataaggc
    11401 tttacaatag agaaggagtg aaactctatt caagcttaga aacgccttcc attagtccga
    11461 aagagacctt agaaagagaa ttaaatcgta aagtatcagg gaaagaaatt caaccgactt
    11521 tggagagaat agagcaaaaa atggttcaaa atcaacacca agaaacccct gaatttaaag
    11581 caattcaaca aaaattggaa agcttgcagc cacctacacc accaatacca aaaataccta
    11641 agcttccagg actttaaacc ttaaaaggtt ctgttgcaaa gttttaaata aagaataaaa
    11701 tcccttacgg tatctatgat ttaagctggg attcccaata ataccttgat ttcagtacag
    11761 accgaaaacc cgaagagagt gccttctttt cgggttttct tatataatcc tcgaatggct
    11821 tccatgcctt taatcgtggg tgaggcagtg cgtaaacttc gatagaattt attgcgtctc
    11881 tttactggac gatggtcttg ttcaatcaaa ttattcaggt atttaatggt acgatgttct
    11941 atcccttgat aaaagccgcc gtattctttt agtttcttaa aggcacttgt aatagagggg
    12001 gctttatctg tgactacaac cttcggttca tcaaactgct tcactaaccg cttaagaaaa
    12061 gcatacgctg cttgtgtgtc ccgtttttta cgtaaccaaa tatccaaggt taaaccatct
    12121 gcatcgatgg ctcgatacaa ataatgccat tttcctttaa ttttgatgta cgtttcatcc
    12181 attttccatg aataaaagga ttttttattt ttctttttcc aaatttgata aagtagtttg
    12241 ccatattctt gcacccaacg ataaatcgtc gtatgacaaa catttattcc acgatcatat
    12301 aacaattcct gaacttcacg atagcttaga ttgtaacgca ggtagtaact aacagcaaca
    12361 ataatgacgt cattttttga attgtttgcc tttaaaatga ttcatcgtca ttcctcctgc
    12421 tatctttttc taatattcta ccttatttga tagtagattt aaaactttgc aacagaaccg
    12481 gttaatctgc gtttttcgct catttttatt gtgaatggga gttttttgat tcccttttga
    12541 agattgaact cgtcaaaagg ttttttgttc ttcggcgcaa ccatcgaata aacgaaaaaa
    12601 cgttgttaag atggaaaata ggtggtataa tataacgata agaggtgaaa gaaatgttac
    12661 tagagactta tctgaagaat aataatacaa atattagaca aatgcatatt gtatcgggca
    12721 ttccagaaac aacagtaaga ggaatcaaca aacgcgactt ttcgaagtgg agcgttacgc
    12781 attttgatgc gattgccagg acggttggta aggagcgaca tgttgttatg caagaattgg
    12841 aagctttgaa agaacaagcg gataatttcg cacctctagg tagatataac ctagaaaatc
    12901 gaaggtatat tggaaataaa aataagttat taaattggat tagtgatcta attgatgcgc
    12961 acactgaagg taattcattt tttgatgttt ttgctggaac aggggtggta tcaaaagaga
    13021 tgcttggaaa atatgattct ttcattatga atgattttct gttttccaat aatattatct
    13081 ataaagcttt ctttagtaat gattcgtttg attcaaataa gttgctctct cgtcaagagg
    13141 agttcaatcg cataattacg aacaaagagg acgacgatta cttttctgat aattttggcg
    13201 ggaagttttt cagtgttcat gatgcaaagg ttataggtga gatacgaaca agaattgaaa
    13261 aagataaatc attgactgaa aaagaacgag caatactgat agcatcactt atttactcga
    13321 gcgataaaat agcaaatacc gttgggcatt acgatgccta tagaaaaaaa gtacaaatag
    13381 ctgataggtt taaatttgag ctggtaaatc ctcttgatac ttcgagtaaa gatatacaga
    13441 ttttcagaga agattcaaat gagttagttc gaaaaatatc aggggatgtg gcgtttatcg
    13501 atccaccgta caattctagg caatactctc gcttttacca tgttttagag ggaatcgcga
    13561 aatgggacaa gccagaatta ggcggtgttg caatgaagcc acctgctgaa aatatgagcg
    13621 attatagccg ttcttcagcg ccaaaagtgt ttgatgattt aattcaaaat cttgacgtca
    13681 agtatattgt tgttacgtat aacaatactt ataaatcgaa aagttctagt tcaaaaaata
    13741 agattacaca tgatgaaata ttggaatctt tgaacaaggt tggaaaaact aaatctttcg
    13801 agatgccttt tcaattcttt aatgcaggaa agacagattt aaaggatcat aaagaattcg
    13861 tctttataac ggaggtagga aaataatgca aacgacatta tttcccactg aaacactcga
    13921 ggttgttgcg cccatgaaaa aaaaaggaaa agctattcgt tcaccgttat tctacgtagg
    13981 tgataagtat aaattgatgc cgcagttgaa agaacttttt cctaaaaaca taaataatta
    14041 ttatgatgtg ttttctggag gggggagtgc ttcaatcaat gtcatagctg ataaaattat
    14101 tatgaatgat gttgacgaaa aggtagttga gctccataga tttttacaag agcaatccag
    14161 tgacattgag ttatttattg agaatatgta cgaacttatt agagaatacg gtttgagtct
    14221 ttcggaatta gggaaaaaca gtgaaattga agaattgaaa aaggagttta agaaaacgta
    14281 ttttgccaaa tacaataagc aggcttactt aaaactcagg gatgactaca ataaaaatca
    14341 gtctaacacc atgcttttgt atctcctcct catatatggc ttcaaccaca tgatacgttt
    14401 caatggtcag ggacagttca atcttcccgt cggtaatgtt gactggaata aaaatgtttc
    14461 aactgcactt agaaactatt cccaatggta taacaacaat aacattgaga tttcaagtgg
    14521 aatggatttt gaggattttg taaattttta tgattttaag aatggtgatt tcctttattt
    14581 cgatccgccg tatctgatta cctttagcga ctacaataag ctttggggag aagatgaaga
    14641 gcggagatta tacaagcttt tggatgaact tgattcgaag gggataaagt gggggctttc
    14701 caatatgtat aatcacaaag gaaagactaa tgaaattttg ctcgaatggg gaaaaaaata
    14761 taatgagtat gacatcaaaa gtaattatat ttcccgattc gataatacga taaaaaaaga
    14821 tagtaaagag atttatatta caaattacac ctatgagtaa tttagattta gagtttcgga
    14881 attagagtgc cacacatttg atgtggtctc ttcctcactt tttaatattt atttggtaag
    14941 gagaagagtg tatgtcgaga gtaagaagaa gaaagccaat tacatttgat acaactttaa
    15001 gaaacccagg tagaataccg caatttatct cggttctcag tgaatttgag gggaaagtta
    15061 ttgatgataa gttagctcta gagttggaag gagaaataat tcggcaaaag atttttgaac
    15121 caactaaagc gaccctaggt acatatatga aggaatataa taaaaaattt cattttgttg
    15181 ctgcggacca atccgaggaa gctgcttcta aggtcgaaga aatatatatt cagtgggaaa
    15241 agggttcgcc tgggagtgtt cctctcgaaa aaataacata tctgttggaa aatacaatca
    15301 cagcacataa agatcgtggg tggaaaggtg gttgggaatc cagaattcat actcaattta
    15361 actttttaaa tgagctcgga tttgttcgag ttgtcaaagg ggaaaagata attatttcga
    15421 aaaatgggaa attaatgatt catgaatatt caaatggata tccaatcaaa gatacctatg
    15481 atgaatcatt tgaacagtca gcgttcttaa atgcgttctc gaaatatcaa gttaacaatc
    15541 cgtatagaag caatactatc aaggtaaact ttttcccatt ggtattgaat gtaataaatt
    15601 atttggatga aaaataccaa cgtcccggca tctcgaaaca agatctacca tttattatta
    15661 cttggggaaa cgatgactat caaacattag cagaatatat ttaccaattc agagttaagt
    15721 ttggctataa cacttcaaat gaattggttt atgaatatgc tatgaaccta ttagatgatt
    15781 ctacgccaaa tgatactatt gcaccagcta ctgtagattt catagagcag aaaaagagag
    15841 attataaatt tgataaaatt attttggaaa ccccagacga agttattcga aagctcagat
    15901 tgaccatgtt aatttctttg cgtggagcgg gacgttttat tgatattaat aaaaatgaat
    15961 tagaaaaaat caagcatgtt ctcgctaact atagctcaaa tatcgatttt gatgatgacg
    16021 tacaaaaata ccttgaatat atgggagaca ttgataattc ccttatgttc gtgtctgatg
    16081 ctcaagaatc acaagaagcc aagtatgcta aagaagttgc aatagaaaaa tgggcaaaag
    16141 agtatgactg gacgttctta aaagaagaaa tggttaatag tgttgaaaaa cgtccttcaa
    16201 gtcatttaat cttaaaatat gtgaaagaaa ctgctagatt ggaattcctt tcagctattg
    16261 taattaaaaa agccttacct aaattgaagg ttgtagctaa ttataaagca gatgatcaag
    16321 gaataccatt taacaccgca tccggtggga agaggaacct aattggtgcg gatattgatg
    16381 tcttcgaaga taacattcac gctattttag aaccaaccat ttctaaacaa agaagtttcc
    16441 aagttgaaca tgaactccct tcaattcgta atcacgtatt aggtactgca aataaagatg
    16501 ttgaagaagg taataatttt aaagaatggt tcagcctgtt tattgcatcc aatataaccc
    16561 gtgatgttgg ggatcaagta gcattgatta aacaaactaa tggtgtcgaa atatatcctt
    16621 gggatatcaa agatttcgtt gaatactctg aacgagttag caccataaga gattataaaa
    16681 aaatcaggaa ttatgtaaaa ccacagagca tgcctgtttt ttaggttaaa taaagaaaat
    16741 tcgtgtctat tcacacccat ttgaacctgt gaatagaacc tttaggaaca attgaacggt
    16801 atgaaactat gtatgcagat gaaccaatga cagacagggc aacaccaaag caagcacatg
    16861 atattgttgt taaaaatcta cctaacaatt tagaattgct taaaaaaacg ggcttattta
    16921 gcgatataag gctatataac agagaaggag tgaaactcta ttcaagtttg gaaacacctt
    16981 ctattagtcc aaaagaaacc ttagaaaaag aattaaatcg taaagtatca agtaaagaaa
    17041 ttcaaccgac tttagaacga atagagcaaa aaatggttct aaataaacac taagaaaccc
    17101 ctgaatttaa agcaattcaa caaaaattgg aaagcttgca gcgacctaca ccaccaatac
    17161 ccaaaacacc tagacttcca ggactttaaa ccttaaaaaa ggaaaagagt agttaccaaa
    17221 aaacggtaac tactcttttt ttataaaaac ctttcctcat gtttgaaaag ctgatcgaga
    17281 aataaagcac acaacggtat tctttctttt ttcagttggt acattttgca gtctgtaacg
    17341 tgtcatttac tgtttgttac cttaacatat ccagtcgaaa acacaagggt atataaacga
    17401 gtaagctcgc ccttgtgttt tctttgtgtc tatgttgcgt tctcgtcaca gcaaacgaca
    17461 cgaacacact ggcgaaaatg aaccaacaaa acgaaaaaag aaaggagcat acactgtaat
    17521 ttatttctag cttttaaaac atgaggaaaa cagcttcact tcctcaaacg cctaacaaaa
    17581 atggttgtca aaaaactaaa aatcaaaaaa agaaaagagg aaaagaaaat ggaaaaaaca
    17641 aaaaaattac aattagaaga ttttacagaa aatgagtttt ttggaacaca agaacagcaa
    17701 tatctaaaag ctcaagtaag agaagagcta aaagagcaag gctttatcat agatagcagt
    17761 tttgagggcg attttaaaac atggataggt gggttctgtt gcaaagtttt aaataaagaa
    17821 taaaatccct tacggtatct ataatttaag ctgggattcc caataatacc ttgatttcag
    17881 tacagaccga aaacccgaag agagtgcctt cttttcgggt tttcttatat aatcctcgga
    17941 tggcttccat gcctttaatc gtgggtgagg cagtgcgtaa acttcgatag aatttattgc
    18001 gtctctttac tggacgatgg tcttgttcaa tcaaattatt caggtattta atggtacgat
    18061 gttctgtccc ttgataaaag ccgtattctt ttagtttctt aaaggcactt gtaatagagg
    18121 gggctttatc tgtgactaca accttcggtt catcaaactg cttcactaac cgcttaagaa
    18181 aagcataggc tgcttgtgtg tcccgttttt tacgtaacca aatatccaag gttaaaccat
    18241 ctgcatcgat ggctcgatac aaataatgcc attttccttt aattttgatg tacgtttcat
    18301 ccattttcca tgaataaaag gattttttat ttttcttttt ccaaatttga tagagtagtt
    18361 tgccatattc ttgcacccaa cgataaatcg tcgtatgaga aacgttaatg ccacgatcat
    18421 ataagatttc ttgaacttca cgatagctaa ggttataacg aagatagtag cccacggcta
    18481 caataatcac atcctgctga aattgctttc ctttaaaatg attcatcgtc attcctcctg
    18541 ctatcttttt ctattattct accttatttg atagtagatt taaaactttg caacagaacc
    18601 tgttttctcc tttcatcaaa gccacaggct ctttttgagc ctgtgctttg cttccttcta
    18661 caatccgcca ttctctcgaa aactatagta acgattgctc gtaacaacga ggtggtcgag
    18721 tagggaaatt cctaataggt cgccagcttc tttgatgcgt tctgtaaata atttatctgc
    18781 ttcacttggt tgcggatttt atcaagagta aattactaaa aatccattaa catcaatgtt
    18841 tttactttaa gaacatatat ttaacgtgtt aaatcaaatg aaaaaataca atgtaattat
    18901 tagatgacaa ttgattacgg tcagaagaat gtaccacaaa taaaaaaact aattcctttt
    18961 tattttatcg aggaattaag cttttgacct gcaacaatca cgcccaattt cggaatatct
    19021 ataatatgat ttcgatctta aattatctac tttaactgac gctacctgct cgttaaagaa
    19081 gacctattta aaaaagttta tactgcacct gcctttatta tgtaagtaaa agtagtaaag
    19141 ctttaaattg gttttcaaaa aagtagggtt gattatttct ttttcatcaa ttataattgt
    19201 gttattatca caatatgaga aaggaggcaa aaagtaatgg gaattgattt cattaatgtc
    19261 gagtttagaa agcatgtaaa agtttcaggg gatttaggta caacgggatc agtatgtcct
    19321 ttttcacaaa aacaaaaaat ggaaacacta ccggaaaact ttttgcctcc ttttatataa
    19381 tgggcaaaaa tttatgaatc tattgcattt gtaaaaatgc atacgaatga ttaggcaccg
    19441 gttgttaaac tggtgccttt tttttgtttg atcagttcag ttagtttgga gaaccgaatc
    19501 tttaatcatg ctgtagaaat atggtgagtt cgttgatttg gttttacaat ataaatagat
    19561 atgaggtgag gtttatggtg aaagcagtgg atttagcctc ctaaaatcac aatatatttt
    19621 aggaggaaat aaaatgaaaa aggatagtaa atctaaagaa atgattcaat ctgaaaaaag
    19681 gggttctact aggcttttaa tgatggtact ctccctatct gtacttgtag gttcaattac
    19741 ggctgattca gtcaatcccg tacttccact aataagcaaa gctttagaag cttcgaaatc
    19801 tcaagtgagt tggatagtta gtggtattgc acttgttctt gcgattggag ttccgattta
    19861 tggtcgaatc tcagactttt ttgagttacg aaagctatat atctttgcca ttatgattct
    19921 ggcaagtggt agtcttttat gtgcaattgc cccgaacctc ccattgttgg ttttgggaag
    19981 aatggttcag ggtgctggga tgtccgcaat tccagttcta tcagtcattg caatttcgaa
    20041 ggttttccca caaggaaaac gtgggggagc tttgggaatt atcgcaggaa gtattggtgt
    20101 tggaactgct gctggtccaa tatttggtgg agtagttggt caatatttag ggtggaatgc
    20161 cttgttttgg ttcacatttt tgttagccat tatgattgtt attggtgcct actacgcgtt
    20221 accgacaatt aaaccggcag aatccgtagg aagcaataag aactttgatt tcattggtgg
    20281 tttattcctc ggcctcacag taggattact cctttttggc atcactcaag gagaaacttc
    20341 tggtttttct tcgttctcat cgttaactag cctaattggt tctgttgtag ctttggtggg
    20401 atttatttgg agaattgtta ccgcagaaaa tccatttgta ccacctgtcc tgttcaataa
    20461 caaggattat gtcaatacgg tcataattgc atttttttcg atgtttgctt atttcgctgt
    20521 tcttgtgttc gtcccattac tagtcattga ggtgaatgga ctctcttctg gacaggctgg
    20581 aatgatattg ttgccaggtg gtgtggctgt tgcaatctta tctcccttcg ttggccgtct
    20641 ttctgatcga tttggggata aacgtctgat aattactggg atgactctga tggggctgtc
    20701 taccttattc ttgtccacct atgcatctgg tgcttcacct ctgttagttt ccgtgggggt
    20761 cctcggagta gggattgctt ttgcattcac gaattctccc gcaaataacg ccgcagtaag
    20821 tgcactcgat gcagacaagg ttggtgtcgg aatggggatt ttccaaggtg ctttgtacct
    20881 tggagcagga actggagcag gtatgattgg agcattatta tccgctcgac gtgatgctac
    20941 tgagccgata aatccattat atatattgga cgctatgtcc tactcagatg cgttccttgc
    21001 agctacaggg gcaatactca ttgccttaat agctggatta ggtttaaaaa agcgtgggta
    21061 ataacttagt tatgtaaaga ttagttattg ggttctgttg caaagtttta aataaagaat
    21121 aaaatccctt acggtatcta taatttaagc tgggattccc aataatacct tgatttcagt
    21181 acagaccgaa aacccgaaga gagtgccttc ttttcgggtt ttcttatata atcctcggat
    21241 ggcttccatg cctttaatcg tgggtgaggc agtgcgtaaa cttcgataga atttattgcg
    21301 tctctttact ggacgatggt cttgttcaat caaattattc aggtatttaa tggtacgatg
    21361 ttctgtccct tgataaaagc cgtattcttt tagtttctta aaggcacttg taatagaggg
    21421 ggctttatct gtgactacaa ccttcggttc atcaaactgc ttcactaacc gcttaagaaa
    21481 agcataggct gcttgtgtgt cccgtttttt acgtaaccaa atatccaagg ttaaaccatc
    21541 tgcatcgatg gctcgataca aataatgcca ttttccttta attttgatgt acgtttcatc
    21601 cattttccat gaataaaagg attttttatt tttctttttc caaatttgat agagtagttt
    21661 gccatattct tgcacccaac gataaatcgt cgtatgagaa acgttaatgc cacgatcata
    21721 taagatttct tgaacttcac gatagctaag gttataacga agatagtagc ccacggctac
    21781 aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtca ttcctcctgc
    21841 tatctttttc tattattcta ccttatttga tagtagattt aaaactttgc aacagaacc
//
