LOCUS       NZ_CP138548             5596 bp    DNA     circular CON 21-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP2094 plasmid pKP2094-5K, complete
            sequence.
ACCESSION   NZ_CP138548
VERSION     NZ_CP138548.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37501186
            Assembly: GCF_033807415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Liu,H. and Li,Y.
  TITLE     Clonal dissemination of ST1049-KL5 carbapenem-resistant Klebsiella
            pneumoniae with a novel hypertransmissible IncM1 plasmid
            co-harboring blaKPC-2 and blaNDM-1 genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Liu,H. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) Department of Laboratory Medicine, Zhongnan
            Hospital of Wuhan University, No.169 Donghu Road, Wuchang District,
            Wuhan, Hubei 430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.5; Canu v. 1.7.1
            Assembly Name                     :: KP2094
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/21/2023 09:48:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,713
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,417
            CDSs (with protein)               :: 5,417
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 71 of 169
            Pseudo Genes (incomplete)         :: 107 of 169
            Pseudo Genes (internal stop)      :: 32 of 169
            Pseudo Genes (multiple problems)  :: 38 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138548.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP2094"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="pKP2094-5K"
                     /geo_loc_name="China:Wuhan"
                     /collection_date="2021-01-28"
     gene            complement(264..422)
                     /locus_tag="SG621_RS25750"
                     /old_locus_tag="SG621_25750"
     CDS             complement(264..422)
                     /locus_tag="SG621_RS25750"
                     /old_locus_tag="SG621_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1062..1238
                     /locus_tag="SG621_RS25755"
                     /old_locus_tag="SG621_25755"
     CDS             1062..1238
                     /locus_tag="SG621_RS25755"
                     /old_locus_tag="SG621_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1228..1572
                     /locus_tag="SG621_RS25760"
                     /old_locus_tag="SG621_25760"
     CDS             1228..1572
                     /locus_tag="SG621_RS25760"
                     /old_locus_tag="SG621_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1781..>2215
                     /locus_tag="SG621_RS25765"
                     /old_locus_tag="SG621_25765"
                     /pseudo=""
     CDS             1781..>2215
                     /locus_tag="SG621_RS25765"
                     /old_locus_tag="SG621_25765"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2250..2759
                     /locus_tag="SG621_RS25770"
                     /old_locus_tag="SG621_25770"
     CDS             2250..2759
                     /locus_tag="SG621_RS25770"
                     /old_locus_tag="SG621_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2766..2978
                     /locus_tag="SG621_RS25775"
                     /old_locus_tag="SG621_25775"
     CDS             2766..2978
                     /locus_tag="SG621_RS25775"
                     /old_locus_tag="SG621_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3354..3482)
                     /locus_tag="SG621_RS25780"
                     /old_locus_tag="SG621_25780"
     CDS             complement(3354..3482)
                     /locus_tag="SG621_RS25780"
                     /old_locus_tag="SG621_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            3645..3869
                     /locus_tag="SG621_RS25785"
                     /old_locus_tag="SG621_25785"
     CDS             3645..3869
                     /locus_tag="SG621_RS25785"
                     /old_locus_tag="SG621_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3853..4119
                     /locus_tag="SG621_RS25790"
                     /old_locus_tag="SG621_25790"
     CDS             3853..4119
                     /locus_tag="SG621_RS25790"
                     /old_locus_tag="SG621_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4189..4539)
                     /locus_tag="SG621_RS25795"
                     /old_locus_tag="SG621_25795"
     CDS             complement(4189..4539)
                     /locus_tag="SG621_RS25795"
                     /old_locus_tag="SG621_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4605..5150)
                     /locus_tag="SG621_RS25800"
                     /old_locus_tag="SG621_25800"
     CDS             complement(4605..5150)
                     /locus_tag="SG621_RS25800"
                     /old_locus_tag="SG621_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(5176..5502)
                     /locus_tag="SG621_RS25805"
                     /old_locus_tag="SG621_25805"
     CDS             complement(5176..5502)
                     /locus_tag="SG621_RS25805"
                     /old_locus_tag="SG621_25805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          897..976
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(314..711)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          1563..3094
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgatgtgca gtgcgctgag ggcatcagtg gcgggtgttt ttccgtggga tggatggagc
       61 tccggggata acagaactga cgatcattta aaggatcttc ttgagatcct ttttttctgc
      121 gcgtaatctc ttgcactgta aacgaaaaaa ccgcctgggg aggcggtttg atcgaaggtt
      181 aagtcagttg gggaactgct taaccgggta actgggtata cgagaccgca gataccaaaa
      241 ctgttctttc agtgtagccg cagttaggca cccacttcaa gacctctcaa tatctctctc
      301 gtatatatcc agttaccaat ggctgctgcc agtggcgttt ttgcgtgtcc gtccgggttg
      361 gactcaagat gatagttacc ggaaagggcg cagcagtcgg gctgaacggg gggttcgtgc
      421 atacagccca gcttggagcg aacgacctaa accgagccga gataccagca gcgtgagcta
      481 tgagaaagcg ccacgcttcc cgaaagggaa aaaggcggaa caggtatccg gtaaacggca
      541 gggtcggaac aggagagcgc acgagggagc cgccggagag aaatcaccgg catctttaag
      601 tcctgtcggg tttcgtcctc ctctgacttg agcgtccatt tctgtgatgc tcgtcagggg
      661 aggcggagcc tatggaaaaa cggcaacgcc gcatcctttt ctccggccct ttctgatttg
      721 agcgcctttg ctttatcccg ttatccgtga gtcggtcacg cccgccgcag ccgaacggca
      781 ggagcgcagc taccgagtga gcgaggaggc gtcatttcat ctgtccgtgc atggtattgc
      841 ggacgctcct gagccacata tcacacctgc cacatgaggc actttcatga atcgtgctct
      901 catgccaaca tagtaagcca gtatacacag cgtaaacgct gtgactggtt cagggctgcg
      961 ccccgaaacc cgctaacacc actgacgcgc tggcgcttgt ccaacgccgg agcgacccgg
     1021 aaaacttttc cgtgccgtcc ggcgtttgcg aaggagggat aatgcagaag ccgaagaaga
     1081 agccgctcgt cgtaaaaaaa agatcctggc atccggcagt gatggcaaat ctgatgcgtc
     1141 aggcacagga ggaagattac cgccgggagc agatggcaga gcttgagcgt tcagcccggt
     1201 cacattttgc ggatatcgga ggagaggatg gcggttaagc gtacccgttt tttaggtatc
     1261 cgggtgaccg atggggagta ccagcagctg ctggagcgct gcaatggaag gcaactggcg
     1321 gtgtggatgc gggaaacctg tctcgatacg cgcccggcgc ggtcgttgcg gctgccgtcc
     1381 atcgatccgg ttttgctgcg ccagcttgcc gggatgggga acaacctcaa ccagatcgcc
     1441 cggaaaatta acggcggcca gtggtcaggc gctgaccggg ttcaggtggt ggccgcgctg
     1501 atggccattg atgccgaact cgagcggctg cggcatgccg tgctggaaaa gggggcagac
     1561 gatgatcgtt aagtttcatc cgcgagggcg aggcggcggt gccgggccgg tggattatct
     1621 gctggggaaa gaccgccagc gcgacggggc catcgtcctg cagggaaagc cggaggaggt
     1681 ccgggagctt atcgacgcct cgccctacgc caaaaagtac acctccggcg tcctgtcttt
     1741 tgctgaacag gatttaccgc ccggccagcg tgaaaagctg atggcgagct tcgagcgggt
     1801 tctgatgccc ggactcgata aagaccagta cagcgtgctg tgggttgaac accgggacaa
     1861 ggggcggctg gaactgaatt ttctggtacc gaacacggaa ctgctgaccg gcaggcgtct
     1921 ccagccgtac tacgaccggg cagaccgtcc gcgcatcgat gcgtggcaga ccgtggtgaa
     1981 cgggcgactg gggctgcacg acccgaacgc accggagaac cggcgcgcgc tggtgacgcc
     2041 atcttcgtta cccaaaacga agcaggaagc cgcagaggct attacgcggg gcttactggc
     2101 cctcgcctcg tcaggggagc ttaaaacgcg tcaggacgtc actgaggcgc tggaaagcgc
     2161 aggttttgag gtcgtgcgca ccacaaagag cagcatcagc attgccgacc cggacggagg
     2221 gcgaaacatc cgacttaagg gagccatcta tgaacagtct tttaacgctg gcgaaggact
     2281 tagagcagaa atcgaaagcg cagcagcaga gtaccggcga aatgctgaaa gccgcattca
     2341 gcgagcacga gaagtctgtc agagcggaac tgagcgaaag cgggaagaga atcagcgccg
     2401 ccatccgcgc ccacgagcag ggtatgagcg cggccatgca gtcgaaccgc ctgagcgtga
     2461 tgcgtatggt cgggcggaca tggctgacca tcacgatggt gtcaggactg ctgttcgcca
     2521 gcctgagcgg ggtgttgtgg tatcagggga gcttgatagc gtcaaatctg gccgaaatcg
     2581 accggcagaa cgcagcgctg tcgaagctga acgcgaagac ctggggcgtg acgtacctgg
     2641 aagacagcaa cggacgtttt ctggtgctgc cgaaggggac ggccgtagac aggacgcaga
     2701 gctggacggt cgggaacggg cggtcgaagc agaacgcgct gagactggtg aaggagtaag
     2761 tgccgatgac cgagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact
     2821 actcgaaaag gctggacgag tgggagagcg ccttcgggga atggcggaaa atgtcagggc
     2881 ttatgcaacg ggagaacgcg gcgctgagcg agcgcgtgac gcgcttgagt cagcaggtgg
     2941 aacgcttgag cgggcaactg cagcgtttga gccggtagtg cagcgtcatg agatggccgt
     3001 ggccgcagaa cgggcgcacg agcaacggca gcacgaaaag gaactgacgg aagcgcgttc
     3061 ccggcagcga agctatgacg gtccgtcgct gggctgaaat gcaggaaaac agggttctca
     3121 tcgggcattt acgctgaaac cggatgtcat tcacctggct gcatggctat gcagccaggt
     3181 aaaatttatc tgtcgcgaac ggggcggttt atcgggtggt ttgttgccgg ttttacctga
     3241 ctgccacccc gttcgcggcg aacccgtccg gggcggtgcg ggcaacgggt gttatgttta
     3301 atcagcgtga acggcagata caaaaaagcc ccggagggct tttaactact gacttatttt
     3361 tttgaagatt tgctgggttt caatttgtca gtatttcccg gcgttttttt attgtcacgc
     3421 tggcgtttat caggttcccc tgttgaatcg gcaattcgtt tcggacccgg cttaagaccc
     3481 ataatcgtta tcctttcaat agttaagagg aaagccctca agctagatat attgaccgcg
     3541 ttacggatta tcaagagggg ctaacaagat tattttttgt tcttttaggt tgattgtggt
     3601 actgttgata tacatgtata gcaaatggtg ttcgggaggt ttatatgctt gctatccggt
     3661 tatctgatga gattgagtcc cgtctggact cgctggcgaa gcaaaccggc agaacaaaga
     3721 cgttttatgc gcgggaagca atactggcgc atctggaaga cctggaggat tattatcttt
     3781 cagcagaaac tgctgcacgc gttcgccgtg gtgatgaagc agtgcattcg tctgaagacg
     3841 tgaggaagtc acttggtctg gacgattaac tattccgatc gggcgctcaa atcgttacgc
     3901 aagatggaca aacagaacgc acgacggatt gtggatttta tgagtttacg cattgcagtt
     3961 gctgccgatc ctcgccagtc agggaagccg ctcaaaggtg agctgggcga gttctggcgc
     4021 tatcgggtgg gagattatcg cgttctgtgt gagatccgag atgacgagct tgttatcctt
     4081 gccgccacga ttggacatcg ccgcgaagtt tacgactgaa gcccgctatg cgggcttttt
     4141 tgatgccgac aacaggtatt atggtaaata ctcgactaac tacgtgttct actgtttgtc
     4201 ggttttagtt aaccagcagg ttgtcttaac gataatgtag ccgttatttt taactaataa
     4261 ggtcatattg ttaacgtcag gcataataaa agacattatg gcctcagagt tatcatcaga
     4321 agcagctctc tgcattacta cttcgattcc cttttctttg aatctataag tatccagttg
     4381 cctattaaac ttagaaagac ctacaaccat acttagtttg ttatctttaa cagtaagaga
     4441 tgcttcctga gtaccattac atcggtagta agcggtgtaa ccgtcagcaa aagatcgacc
     4501 ggggaatata aatgctagga acgcaattaa ccaatacata gttgagaatc ttggtattac
     4561 acatttcact atcctctctt tcatttttcg ttcaccatac tttgttatct gatttttaat
     4621 ttttctattt tataaaccaa gtctctacca aaaaggcgaa aattatcctc aaataataat
     4681 aattgtttaa gaacatccat gaaagcagct gcttcaccct cagttatggg agctactcta
     4741 tataacatat aacctgagtt aagggtttct tttatacttt cttcaatgct cattataaat
     4801 attagtggat tatcattttt cttcccccat gggtttctaa ttaatccttc gatgtactca
     4861 ggtcctttgc gtcctattgc ctgcgcaata ttttttattt catctgcgtg agggctttgt
     4921 tgtacagctt tgtttatgcc tgcttgagtg attttcagag actctacacc gtgtatgttg
     4981 tatgagataa aatgtaataa attaagatgc aatgcaatta atccattttt gtttacatct
     5041 gagtatttgg ataaaaaacg taatgaagtg ttaactctcc cttgaagcct aacaattggg
     5101 agtgctgcct tgtatatttc cttctccgtt aaataccgtt taaaaaacat aattctccgc
     5161 ctccgtatag cgtgcctatt ttatttcgtc gtaaaactta gctctgaaat attcctttag
     5221 aaccatcatg tcattaataa ttgaatctat ctcatccatt ttttcttggt attcttttaa
     5281 tggtgaaggg agcggtaact caatatttga aaagtgattg gcgagatagg gatcaaggtt
     5341 tctcatccat tcaggctttt cgccaatgcg ttcttcaact gaaagtgaac ctgataatct
     5401 ctggttaata agatcactta gactcatttt ggttttattc gaaagccgtt gtaaggattg
     5461 ctttaatcct atatctagca tgatatttgt tctttctctc attacccccc cttgctattg
     5521 cgttttttgt tgcgcacaaa gctaccattg cgcattaaga aacgcaataa aaaaggaggg
     5581 agggagtggt ttgtac
//
