LOCUS       NZ_CP138546            10060 bp    DNA     circular CON 21-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP2094 plasmid pKP2094-10K, complete
            sequence.
ACCESSION   NZ_CP138546
VERSION     NZ_CP138546.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37501186
            Assembly: GCF_033807415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,H. and Li,Y.
  TITLE     Clonal dissemination of ST1049-KL5 carbapenem-resistant Klebsiella
            pneumoniae with a novel hypertransmissible IncM1 plasmid
            co-harboring blaKPC-2 and blaNDM-1 genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,H. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) Department of Laboratory Medicine, Zhongnan
            Hospital of Wuhan University, No.169 Donghu Road, Wuchang District,
            Wuhan, Hubei 430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.5; Canu v. 1.7.1
            Assembly Name                     :: KP2094
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/21/2023 09:48:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,713
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,417
            CDSs (with protein)               :: 5,417
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 71 of 169
            Pseudo Genes (incomplete)         :: 107 of 169
            Pseudo Genes (internal stop)      :: 32 of 169
            Pseudo Genes (multiple problems)  :: 38 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138546.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP2094"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="pKP2094-10K"
                     /geo_loc_name="China:Wuhan"
                     /collection_date="2021-01-28"
     gene            join(9693..10060,1..4)
                     /gene="tolA"
                     /locus_tag="SG621_RS25110"
                     /old_locus_tag="SG621_25110"
     CDS             join(9693..10060,1..4)
                     /gene="tolA"
                     /locus_tag="SG621_RS25110"
                     /old_locus_tag="SG621_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1..366
                     /locus_tag="SG621_RS25115"
                     /old_locus_tag="SG621_25115"
     CDS             1..366
                     /locus_tag="SG621_RS25115"
                     /old_locus_tag="SG621_25115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            541..819
                     /locus_tag="SG621_RS25120"
                     /old_locus_tag="SG621_25120"
     CDS             541..819
                     /locus_tag="SG621_RS25120"
                     /old_locus_tag="SG621_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            807..1121
                     /locus_tag="SG621_RS25125"
                     /old_locus_tag="SG621_25125"
     CDS             807..1121
                     /locus_tag="SG621_RS25125"
                     /old_locus_tag="SG621_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1285..1713
                     /locus_tag="SG621_RS25130"
                     /old_locus_tag="SG621_25130"
     CDS             1285..1713
                     /locus_tag="SG621_RS25130"
                     /old_locus_tag="SG621_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1739..1918
                     /locus_tag="SG621_RS25135"
                     /old_locus_tag="SG621_25135"
     CDS             1739..1918
                     /locus_tag="SG621_RS25135"
                     /old_locus_tag="SG621_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1945..2475)
                     /locus_tag="SG621_RS25140"
                     /old_locus_tag="SG621_25140"
     CDS             complement(1945..2475)
                     /locus_tag="SG621_RS25140"
                     /old_locus_tag="SG621_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2482..3213)
                     /gene="mobC"
                     /locus_tag="SG621_RS25145"
                     /old_locus_tag="SG621_25145"
     CDS             complement(2482..3213)
                     /gene="mobC"
                     /locus_tag="SG621_RS25145"
                     /old_locus_tag="SG621_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(3213..5177)
                     /locus_tag="SG621_RS25150"
                     /old_locus_tag="SG621_25150"
     CDS             complement(3213..5177)
                     /locus_tag="SG621_RS25150"
                     /old_locus_tag="SG621_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6661..6810)
                     /locus_tag="SG621_RS25155"
                     /old_locus_tag="SG621_25155"
     CDS             complement(6661..6810)
                     /locus_tag="SG621_RS25155"
                     /old_locus_tag="SG621_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6895..7152)
                     /locus_tag="SG621_RS25160"
                     /old_locus_tag="SG621_25160"
     CDS             complement(6895..7152)
                     /locus_tag="SG621_RS25160"
                     /old_locus_tag="SG621_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7162..8847)
                     /locus_tag="SG621_RS25165"
                     /old_locus_tag="SG621_25165"
     CDS             complement(7162..8847)
                     /locus_tag="SG621_RS25165"
                     /old_locus_tag="SG621_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9174..9419
                     /locus_tag="SG621_RS25170"
                     /old_locus_tag="SG621_25170"
     CDS             9174..9419
                     /locus_tag="SG621_RS25170"
                     /old_locus_tag="SG621_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             2162..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5980..6558
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2486..3213)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atgaaagctt ttactctgac agtgctggcg gccatgttcc tcactccggt acatgcgata
       61 ccactaaaac ccgtaaccac actgcagcca acctacccgg tacaggccgc agcaaaacgc
      121 atcgaaggtc aggtgaccgc cacgtttgat attctggacg atggacgagt ggacaacgtg
      181 tcgcttaccg gctgtcacct tttttttcgg gaaacgctga cggcaatgag gcactggcta
      241 tatcccgcag ggcacccggc aatgcgacag gttataaatt ttgaattcag tctgaaaaat
      301 gacggcttac aggatccgcg accagtcacc acccaaaagc tcaatgatga tggttgccgc
      361 cagtaacagg tcctttaacc cgcaggctgc aatagtcctg gatgatgagt gagcctgtag
      421 cagccctgat tagtggaagc gaagctgaaa aatgggcagc aaaagccgta tttctggcaa
      481 atagcgccgc cacggattaa agtagtcatg tgtaatacat atgactacaa aggggctttt
      541 atgtcatcag caacatccat caggctcgat gaagaactta aggaccggct gaaaacgctg
      601 gccgatgacc ggcaccgctc agcgcacgcg ctaatgctgg aggcaatcac cgaatacatc
      661 gaccgggaag agaagcgcag ccagtacctg cgggacggac aggccgcatg gcagcactat
      721 caggaaacgg gtctgcacct tactgcggaa gaagcggaag cctggataag tacctgggga
      781 actgagaacg agcaggatgc tccgccatgc cacaggtaac gatttccgcg ctggcacagc
      841 gcgatttaca acgccttcag gactttctca aaaccaaaaa tcggctggca gccagaaaag
      901 gaggtgaggt gatcgtccgg gctatccagc aactgaaaac attgccagac attggccgcc
      961 cggtgccgtt tctgccgctg gaatataagg aactggtgat cgggtttggc gacagtggct
     1021 atgtgatgct ctaccgccac gaccgggaaa tggaccagat tgtgatcgtc accgtcaggc
     1081 atcagaaaga atccgggtat ccgggggcag acagcctcta ataaccgatg acgggggcgc
     1141 aattcattgc gtggccgtcg ccggtttgac aagcgcagcg cgtcagaaaa ccacatcctg
     1201 aacggaaaaa cgggtaaaat tcagcgatac atttaccgtg agaaattaca cacctttcac
     1261 acccgataaa atcaggggta tacagtggcg aaaacattgc tcaacctttc acaggcagca
     1321 caagccgcgg gcattaccag aagaacgcta tacaatcatg taaaacaggg aaaggttacc
     1381 gtgtcacgtg acgggaaaaa caaccctgtc gtggatgtgt cagaactgat ccgtgtatac
     1441 ggaaatgtga atatccctga gaaacaaatt cccggcattt cacaccgtga aaatacacaa
     1501 aaaaatttcc cacaggaaca gcttctggca atgcaaaagg aactggccga tctccggcag
     1561 gctgtcaccc tgatgctgga agataaaaca tcccgggaag aagaacgccg ccagcacgat
     1621 gatgagcgcc ggaaactcca ggccgaagtg gacagattga caacagaact gacacagaag
     1681 aaaaaacgct tctggtccgg ctggtttagc tgagaactta ccgaagggaa tggagaacat
     1741 gacgaagcag gaaaaaactg ccctcaatat ggctaaattc attaaggatc agtcgctgct
     1801 gttgctggaa aagctgaacg agctggacat ggataccccg gccgacatgt gcgaacggct
     1861 tcacgaggat gcggaaacac tctggcaggt gatgcgggat aagctcgggg aagaataaca
     1921 gcaccggcca tgccggtgct ggcgttattt tttgccgaac ggccaccacc ggcgggcggg
     1981 tttgtcctcc ggcagttcag gcaccggcac gttaaaatag cggctgttat attcccgcat
     2041 cagctccgct ttcagcttct cgttcaccgc gctttccccg tgccgctcgc tgaccacctc
     2101 aatcatcgcg ttaatctgct ccggtgagca cttcccggca tcaaccacct gcacaccgtt
     2161 ctcgatggcg aaccgctcca cctttcgata gaggtaatca cacagcttca ttcctgtggg
     2221 tttgtcccgt tcccattgcg gattttcacc ggtgaaaaag tacgtggctt tttcgtcgct
     2281 gttgagccgc tcgtcataca tcccgatgga tttcatcatc gccagatgct gggcccggtc
     2341 gacaaaacag aaaacatacg ccccggtctg ccaggcgagc accaccatgt cttccagatg
     2401 ctcgctggtt ggcataatgc agcgctcgcc gtctttgtct tccacgtcct caataaatac
     2461 catgtacagc gccataatcc ctcactccgt tgtcataaat ttaaagcacg cgaacagcct
     2521 ctcccggtcc agctccaccg ttttgcgggc ctcgccggtc tgtggattca cgatttcatc
     2581 caccgccaga atcgcccgct gtacccggac aagcgtcggc cggtcagggc agatgtacca
     2641 gatttcatcg atgccgttcg cccggcggtt gaacagatac ccggacacca cttcctgata
     2701 ccgcctgcgg ctcttgacgg tgcgctctac ctcaaatgcc accgttctgc cgtccagctc
     2761 cgccagcgcg tccggcactt tcatcccttt cagggccata cggtgcaggt accgccagcc
     2821 ggtggccccc cggccctcca tggagaggcg cagggactgc acggccagcc ggtgcggcag
     2881 ctgcgccgcc gacacccttg acggctgaaa cggaataacg tccgtcagcg gctcgtcctc
     2941 gtcgaacgag aacgccaccc cgtgcggcgt cagcccccac acgctgacct gacggccgtc
     3001 aaccgggtat ttcgcccgga ccagcagccc tttcgccacc agactgtcca ggaagcggta
     3061 aaccggctgt accgccgcaa tccccagccg tcgcgcaacc agctccgccg tggaacacag
     3121 ctcttcccgc agccagcgca gggtcagccg tgttttctcc gcgttccgcg cctgccgctt
     3181 cgcgtggctg acgttgtact gctccagtgt catcagtaaa gccccgcaaa atcatcatcc
     3241 gcttttggtc cgccgccgtc ggccggaggc aggtactccg ccagcgggtc cgttttcgcc
     3301 agcaccggga aggttttcag cgaaatttcc cggcgctcaa cccgcagcgg gctgctgaac
     3361 gccagccgcg ccacgcccag cccggtcatc accgcgaacc cgtcaggcag gtgttgcagg
     3421 gtgttcacgt caaacagcgg ccgcgcctcc ttctggatga agctgtcgcc gctgatgtgc
     3481 tccacgttcc ccgcctcccg gctggcccgg cggcgctcca cgtccacccg gatctccccg
     3541 gtctggcccg ccgcccactg cgcggactcc tggctggccg cgcgatagaa ccagcggatc
     3601 ggcgtgttat ccagtaccgt ggttttcaca aaatcagcgg gcagatcctg cccgcactga
     3661 ccgaagtcgc ccagcgactg gtgcgcgagc agcaggttgc agtcccggct gcgcagcgtc
     3721 cccagcgcgt tcagcacgta cttcgacagc agatatttaa tctcatcgag catgatgctc
     3781 gcgtgcggcc acctccggaa ctcatcccgc gcaatgatga tttgcgcgca gcgggcaaac
     3841 agcatcttct gcacccggat caccgcctca tcgtccatcg agccgatgac gtacaggcag
     3901 ccgccgccgt tgattatccc cgccacatcg ataccgctgt ccgtctgcag gacgctcagg
     3961 tcggccacgt tctccagctt ggtgatcagc cccttcacgt cctttttcag ggcctccggc
     4021 aacgcgtacg ccgccgccag cacctgctga atgtttgccc cctgcgggaa ctgctccgca
     4081 atcagcttcg ccgctttctg ctcgctgatg cggtagtggt ccgccacgtc gccggtctca
     4141 ctcaggttaa acccggcaac cagcagattc ttcagggcgt actggtcgag atcgcggaac
     4201 agattcagct gcgccggttt ccccggccgc agatccagca gggtgaagtc cgggcagtgc
     4261 gcccggaaga cgtgcggcgc ccacgcgtcc cctttggggt cgaagtacac cacagcgtcc
     4321 ccgaaatcgc gtacgcagcg aatgagcgcg ttggtggcca tcgcgctctt gccgctcccg
     4381 ggcaggccga ggatctgaat attggtcttg cgccaggtat cacgagggac cagcaccggc
     4441 tggccctgct catccttacc cagaaacacc gcctgctgtt tccgggcctg ctccgtggaa
     4501 aaatcggcca gcgcgttaac cggtaccggc tccccgaaat gcaggtggcg gacgtctgtc
     4561 atttctccgg tccgctggct gctgcgccgg ctctcctgat tcagtttcgc gttcaccgcc
     4621 tcgtcagcgg ccgcaaaatg accatcaagc agcaggaacg ccagcagccg cgccagaagg
     4681 acgccggcga caagccctcc ggcacaccag gctatcccga acaggttagc cggctgcagc
     4741 aggtaataat ctgcgttcgc atagaggtcc gggcgcagga cggtttctcc ccagctctcg
     4801 ataaacacgg cgacaaccag cccccccaga agggccagac cggcagcgcc ggtcagccag
     4861 tacgcgtagt tccagacatg tcgccgtgcc ctgaccatcc gcccgggtga atccagcgag
     4921 gccagcaccg aaaatggcac agtccggtta ttatcgcgct tatagcgatc ggcatactcc
     4981 cgccgcgcct tatggctcag ccccacaaaa cgcattcggc cggcccagag ttcagccaga
     5041 ccgaaaaaca ggccgataac cagaaaaaaa ccggccagac tgtaatactc ccggtacagc
     5101 cagctgaccc acgggtaaat ctgccgggcc agctccggga tatgccactg ccaggcaagc
     5161 gaatccgtat taaacatgca atctctctcc ttcagacgac gggtttaccg gcgtgatgcg
     5221 cagcccccgc tgcacaagcc gcggacatgc cccaaggcat ggcggcggcg gtcacgtccg
     5281 tggtgattgc gttcagcttt caccgcggag gccagcctct ttccgcccca tgcggaaaag
     5341 gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag gcggcacccc
     5401 agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga gcggctgacc
     5461 acccgcaaaa tcaggagcag ctttgctgct tcgtcaggtg cgtccagcac cgtggatttt
     5521 tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa tttctggcgt ggccggcgtc
     5581 cagccggaca gagccagaaa gaggggaaaa gaccgtgcgt cggtggcgtc cagccagcgt
     5641 aagcaaggtc ttttcccctg aagtcagcca cgccccagcg gggatgactt gcatttctcc
     5701 gcaaggagaa aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa aaaccgacat
     5761 ggttcaccta tcctgataat tgatcgtcag ggaatagaaa gacttaatca ggggacaata
     5821 tcccacatca gcgcgtccgg tgaccgtctt gcaggtgccg gggaaattgc gcctcctcgc
     5881 tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac acatacaaag
     5941 ttacccacag attccgtgga taagcagggg actaacatgt gaggcaaaac ggcagggccg
     6001 cgccggtggc gtttttccat aggctccgcc ctcctgccag agttcacata aacagacgct
     6061 ttttcggtgc atctgtggga gccgtgaggc tcaaccatga atctgacagt acgggcgaaa
     6121 cccgacagga cttaaagatc cccaccgttt ccggtaggtc gctccctctt gcgctctcct
     6181 gttccgaccc tgccgtttac cggatacctg tccgccgttc tcccttgcgg gaagtgtggc
     6241 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
     6301 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca
     6361 cttgagtcca acccgggaaa gcacgcaaaa cgccactggc agcagccatt ggtaactggg
     6421 agttcgcaga ggatttgttt agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc
     6481 cggctacact ggaaggacag atttggttgc tgtgctctgc gaaagccagt taccacggtt
     6541 aagcagttcc ccaactgact taaccttcga tcaaaccacc tccccaggtg gttttttcgt
     6601 ttacagggca aaagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6661 ctactgaacc gcgatccccg tcagttcaga ggaggacgat ggtgccaccg ttccgccctg
     6721 aacatcccgg atataatttg cctgacatgc ggccagaaga aagccagcaa caatcaatat
     6781 aaaaagaaaa atagtcttta cctttttcat atactttccc tgtatttaaa gactccggca
     6841 ttcatccctg aacaccggaa gtcagtattt caaatcatcc ttgataattt tttgttacca
     6901 gttaccgtcc cgataatcaa aagctatata acaattatcc acatcaggtt ttatttgatt
     6961 tttaaaaaat ggctgtagta aaggaaccca ttctttcttc aaatcaaact caccattatt
     7021 aatattatcc tttaaaggca ttcccaagct ttcaatgaca gaaccatcgt caccaaagtc
     7081 tttagaatac tcttcccctt taaactcttc agttttctta tcaaaccatg tcaaacgtaa
     7141 tttaagcccc atatttacct ctcaaagata ttttttaatg ttccgttttg gatctggccc
     7201 tttaacctgc ttacccgttt tgggatcaaa tgcgcctagg tgttcgccat cactggcacg
     7261 atacccttca agctcaccgt gctgggaatc ccattcataa attttacgtt ttttatcgcc
     7321 ataccatcgg gcccgcttac caccaccacc ttgtttaggt gttttaggat cacctcgctt
     7381 taactctcca agtcccttta tgtcttctgt tttaggtact ggatgataat catggccgta
     7441 gtctttagtc cccttgcgag gcttgttctt ttcctcattt aacttatttt ctgcatcctt
     7501 cgcttttttc tccttttgtt tccggctctc catcgccccg gtaagtgcag ccttatctct
     7561 ggataaggat tcattcattg cgtttaaaga tgcctgagcc gcttttaatt tctcattctg
     7621 attctgaata tccttatcaa gatagcgaac ctgatcctgc cacccggagc catattcatg
     7681 aggcgggtta taagtcacaa aatcatcttt atttgcctct gcctctttta cctttttatc
     7741 ggcttcctga acagcagccc ttgcaccggg gagcgccttc tctgtcgatg caatgcggct
     7801 gtttaaggtc gcaatatttt tatcttcggc atccagctca gctttcgctt tatcatagtc
     7861 tcttttcagc ccctcttccg gatgagcggc gtcccatgcc tgctggcgac gcttttcttc
     7921 ctcctgacgc tgttttacct gtgccggggt aagaacatct gtcaccgaca cataaaccgg
     7981 cttctgtccg gtctctttcg ggaaacgaat aacggcctca cgggagttac caccggctgt
     8041 aaaaccagcc ggacgtgaat cacctttttc agcaacaatg ccttttggcg gggctttcgc
     8101 tgtcggaaca cctttcggta cgctcacctg cagagacggg agacccggaa tcgacacact
     8161 gaataccccc ggacgttttg tcggtttcgc atccaccaca gggacactca ttggccgacc
     8221 tgcgacaacc gcaatatgct gccgctcatc cttcaccaca tccacaaccc gttgtctgac
     8281 acgaaccgtc gcctggtcca gtggtaaagt acttgccgga gtctccgtca ccgcatcggc
     8341 cggtaatgac gtcataattt ttgacatcat tttcggatca tcttttgcaa tctcagaagg
     8401 cagcacaccg tagatcgcaa tcccccacag accaaactta aacggcccct tcagggcagc
     8461 cagcacatca gcaacggcgg ccgacaacgc atcgccggaa actgataaag ccagtccttc
     8521 agcccccgga gtagccaggg caggcagacc gaaggccatc gcagtggccg aagactgtcc
     8581 gccaccagag ctaccgctat gattgccgct gttaccacca ttaccaccgg gtttactccc
     8641 gccgttacca aactcccccg gattatagtt atgaatatct ccattcggcg tatgcgtcgt
     8701 accccagccc ggcccgctgt ttggatccgt accaccacct gatgaagggc cacctttacc
     8761 agacgtaccg ttcacattcc cactggcctg accaccatta tgtcccagac ctgaattacc
     8821 cggacctcga ccgtctccac cactcatacc gttttcctct ttgacattta aaacaataaa
     8881 ttaaagccaa catactgtac atatacacag tataattatt accttactcc tcccgttttc
     8941 aggtgtcaat atatgagatt agttctcatc ttcaggcaca aaaaagcgcc cggagccacc
     9001 tgtaagcaga tcgcgtcttt tcccgaaaat ttctcatggc aacaaatttt attatttaag
     9061 acattcatca cacttacatt tcagatggta tagacacatc tgaaagctaa ctggcatgag
     9121 gagaggacct gcggttaaac tgttcgggaa acctgagacg cccggaggcc actatgacgc
     9181 acctgaaact gactcccgct gaagaaaagc gcctggcgga cgcctttgtc gctcaggcac
     9241 tggccatggg cctgaaaggg aacaaaacgc tgccaaaggc ggtaaaacaa caggtgcggg
     9301 aaagggtgct tcgtgccatt caggacgaac gtgaagccag gcgggcgcag gaaatgcgtc
     9361 gggagaaata cgaggctacg gaaaccacct tcacctggca gccacagcgc cgccggtaag
     9421 gggtatttac cataacgtcg cattgcccgc accgctcaga cggtctgagg gcgtttccgg
     9481 cggctacggg ataaaacagg caacaaacca cccggaaaac cgccggaaac gcgcccggta
     9541 aattgtaacc cgctgcatgc ctatgcacgc aactgcatgg cgggccgttt ggtgtaaaaa
     9601 cgatacgata agccgtgttt atgtaaatga acgtggccgt aaagagtgac aaatatacat
     9661 gtaacatttt ttactgacag gtcatcaacc cgatgcgttt aatacccttt atcctgctgg
     9721 cattgtgcag ccccttactc ctgacggcct gtaagcattc caccacccag ccgccgaaaa
     9781 tttatactct ggataagtca tctaaaatca gtagctacgc tgcggccatc aagaccgctg
     9841 ttgaaatgca gatgtttgaa ccagaggcct atgcgggaaa aatgtgcacc gtgcggatat
     9901 ccatacaacg tgatggcact ctgaacagcg ccattgctga aggaggagat ccgaaactct
     9961 gtaaagcagc aatatcagcc gttacccacg ccaaaattcc gccggcaccg gatgaaaata
    10021 cctggcaaat attcaaaaac gcccctctgg atttcagacc
//
