LOCUS       NZ_CP138541             5596 bp    DNA     circular CON 21-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP1078 plasmid pKP1078-5K, complete
            sequence.
ACCESSION   NZ_CP138541
VERSION     NZ_CP138541.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37501187
            Assembly: GCF_033807395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Liu,H. and Li,Y.
  TITLE     Clonal dissemination of ST1049-KL5 carbapenem-resistant Klebsiella
            pneumoniae with a novel hypertransmissible IncM1 plasmid
            co-harboring blaKPC-2 and blaNDM-1 genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Liu,H. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2023) Department of Laboratory Medicine, Zhongnan
            Hospital of Wuhan University, No.169 Donghu Road, Wuchang District,
            Wuhan, Hubei 430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.5; Canu v. 1.7.1
            Assembly Name                     :: KP1078
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/21/2023 09:47:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,733
            CDSs (total)                      :: 5,605
            Genes (coding)                    :: 5,435
            CDSs (with protein)               :: 5,435
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 71 of 170
            Pseudo Genes (incomplete)         :: 106 of 170
            Pseudo Genes (internal stop)      :: 33 of 170
            Pseudo Genes (multiple problems)  :: 38 of 170
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP1078"
                     /isolation_source="sputum"
                     /db_xref="taxon:573"
                     /plasmid="pKP1078-5K"
                     /geo_loc_name="China:Wuhan"
                     /collection_date="2021-06-15"
     gene            1..546
                     /locus_tag="SG622_RS25760"
                     /old_locus_tag="SG622_25760"
     CDS             1..546
                     /locus_tag="SG622_RS25760"
                     /old_locus_tag="SG622_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            612..962
                     /locus_tag="SG622_RS25765"
                     /old_locus_tag="SG622_25765"
     CDS             612..962
                     /locus_tag="SG622_RS25765"
                     /old_locus_tag="SG622_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1032..1298)
                     /locus_tag="SG622_RS25770"
                     /old_locus_tag="SG622_25770"
     CDS             complement(1032..1298)
                     /locus_tag="SG622_RS25770"
                     /old_locus_tag="SG622_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1282..1506)
                     /locus_tag="SG622_RS25775"
                     /old_locus_tag="SG622_25775"
     CDS             complement(1282..1506)
                     /locus_tag="SG622_RS25775"
                     /old_locus_tag="SG622_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1669..1797
                     /locus_tag="SG622_RS25780"
                     /old_locus_tag="SG622_25780"
     CDS             1669..1797
                     /locus_tag="SG622_RS25780"
                     /old_locus_tag="SG622_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(2173..2385)
                     /locus_tag="SG622_RS25785"
                     /old_locus_tag="SG622_25785"
     CDS             complement(2173..2385)
                     /locus_tag="SG622_RS25785"
                     /old_locus_tag="SG622_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2392..2901)
                     /locus_tag="SG622_RS25790"
                     /old_locus_tag="SG622_25790"
     CDS             complement(2392..2901)
                     /locus_tag="SG622_RS25790"
                     /old_locus_tag="SG622_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2936..3640)
                     /locus_tag="SG622_RS25795"
                     /old_locus_tag="SG622_25795"
                     /pseudo=""
     CDS             complement(<2936..3640)
                     /locus_tag="SG622_RS25795"
                     /old_locus_tag="SG622_25795"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3579..3923)
                     /locus_tag="SG622_RS25800"
                     /old_locus_tag="SG622_25800"
     CDS             complement(3579..3923)
                     /locus_tag="SG622_RS25800"
                     /old_locus_tag="SG622_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3913..4089)
                     /locus_tag="SG622_RS25805"
                     /old_locus_tag="SG622_25805"
     CDS             complement(3913..4089)
                     /locus_tag="SG622_RS25805"
                     /old_locus_tag="SG622_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4729..4887
                     /locus_tag="SG622_RS25810"
                     /old_locus_tag="SG622_25810"
     CDS             4729..4887
                     /locus_tag="SG622_RS25810"
                     /old_locus_tag="SG622_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            5245..5571
                     /locus_tag="SG622_RS25815"
                     /old_locus_tag="SG622_25815"
     CDS             5245..5571
                     /locus_tag="SG622_RS25815"
                     /old_locus_tag="SG622_25815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          complement(4176..4252)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          4441..4838
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2058..3589)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtttttta aacggtattt aacggagaag gaaatataca aggcagcact cccaattgtt
       61 aggcttcaag ggagagttaa cacttcatta cgttttttat ccaaatactc agatgtaaac
      121 aaaaatggat taattgcatt gcatcttaat ttattacatt ttatctcata caacatacac
      181 ggtgtagagt ctctgaaaat cactcaagca ggcataaaca aagctgtaca acaaagccct
      241 cacgcagatg aaataaaaaa tattgcgcag gcaataggac gcaaaggacc tgagtacatc
      301 gaaggattaa ttagaaaccc atgggggaag aaaaatgata atccactaat atttataatg
      361 agcattgaag aaagtataaa agaaaccctt aactcaggtt atatgttata tagagtagct
      421 cccataactg agggtgaagc agctgctttc atggatgttc ttaaacaatt attattattt
      481 gaggataatt ttcgcctttt tggtagagac ttggtttata aaatagaaaa attaaaaatc
      541 agataacaaa gtatggtgaa cgaaaaatga aagagaggat agtgaaatgt gtaataccaa
      601 gattctcaac tatgtattgg ttaattgcgt tcctagcatt tatattcccc ggtcgatctt
      661 ttgctgacgg ttacaccgct tactaccgat gtaatggtac tcaggaagca tctcttactg
      721 ttaaagataa caaactaagt atggttgtag gtctttctaa gtttaatagg caactggata
      781 cttatagatt caaagaaaag ggaatcgaag tagtaatgca gagagctgct tctgatgata
      841 actctgaggc cataatgtct tttattatgc ctgacgttaa caatatgacc ttattagtta
      901 aaaataacgg ctacattatc gttaagacaa cctgctggtt aactaaaacc gacaaacagt
      961 agaacacgta gttagtcgag tatttaccat aatacctgtt gtcggcatca aaaaagcccg
     1021 catagcgggc ttcagtcgta aacttcgcgg cgatgtccaa tcgtggcggc aaggataaca
     1081 agctcgtcat ctcggatctc acacagaacg cgataatctc ccacccgata gcgccagaac
     1141 tcgcccagct cacctttgag cggcttccct gactggcgag gatcggcagc aactgcaatg
     1201 cgtaaactca taaaatccac aatccgtcgt gcgttctgtt tgtccatctt gcgtaacgat
     1261 ttgagcgccc gatcggaata gttaatcgtc cagaccaagt gacttcctca cgtcttcaga
     1321 cgaatgcact gcttcatcac cacggcgaac gcgtgcagca gtttctgctg aaagataata
     1381 atcctccagg tcttccagat gcgccagtat tgcttcccgc gcataaaacg tctttgttct
     1441 gccggtttgc ttcgccagcg agtccagacg ggactcaatc tcatcagata accggatagc
     1501 aagcatataa acctcccgaa caccatttgc tatacatgta tatcaacagt accacaatca
     1561 acctaaaaga acaaaaaata atcttgttag cccctcttga taatccgtaa cgcggtcaat
     1621 atatctagct tgagggcttt cctcttaact attgaaagga taacgattat gggtcttaag
     1681 ccgggtccga aacgaattgc cgattcaaca ggggaacctg ataaacgcca gcgtgacaat
     1741 aaaaaaacgc cgggaaatac tgacaaattg aaacccagca aatcttcaaa aaaataagtc
     1801 agtagttaaa agccctccgg ggcttttttg tatctgccgt tcacgctgat taaacataac
     1861 acccgttgcc cgcaccgccc cggacgggtt cgccgcgaac ggggtggcag tcaggtaaaa
     1921 ccggcaacaa accacccgat aaaccgcccc gttcgcgaca gataaatttt acctggctgc
     1981 atagccatgc agccaggtga atgacatccg gtttcagcgt aaatgcccga tgagaaccct
     2041 gttttcctgc atttcagccc agcgacggac cgtcatagct tcgctgccgg gaacgcgctt
     2101 ccgtcagttc cttttcgtgc tgccgttgct cgtgcgcccg ttctgcggcc acggccatct
     2161 catgacgctg cactaccggc tcaaacgctg cagttgcccg ctcaagcgtt ccacctgctg
     2221 actcaagcgc gtcacgcgct cgctcagcgc cgcgttctcc cgttgcataa gccctgacat
     2281 tttccgccat tccccgaagg cgctctccca ctcgtccagc cttttcgagt agtcctgctg
     2341 tagctgctct aatgcgctca gcaactgttt ttccagctcg gtcatcggca cttactcctt
     2401 caccagtctc agcgcgttct gcttcgaccg cccgttcccg accgtccagc tctgcgtcct
     2461 gtctacggcc gtccccttcg gcagcaccag aaaacgtccg ttgctgtctt ccaggtacgt
     2521 cacgccccag gtcttcgcgt tcagcttcga cagcgctgcg ttctgccggt cgatttcggc
     2581 cagatttgac gctatcaagc tcccctgata ccacaacacc ccgctcaggc tggcgaacag
     2641 cagtcctgac accatcgtga tggtcagcca tgtccgcccg accatacgca tcacgctcag
     2701 gcggttcgac tgcatggccg cgctcatacc ctgctcgtgg gcgcggatgg cggcgctgat
     2761 tctcttcccg ctttcgctca gttccgctct gacagacttc tcgtgctcgc tgaatgcggc
     2821 tttcagcatt tcgccggtac tctgctgctg cgctttcgat ttctgctcta agtccttcgc
     2881 cagcgttaaa agactgttca tagatggctc ccttaagtcg gatgtttcgc cctccgtccg
     2941 ggtcggcaat gctgatgctg ctctttgtgg tgcgcacgac ctcaaaacct gcgctttcca
     3001 gcgcctcagt gacgtcctga cgcgttttaa gctcccctga cgaggcgagg gccagtaagc
     3061 cccgcgtaat agcctctgcg gcttcctgct tcgttttggg taacgaagat ggcgtcacca
     3121 gcgcgcgccg gttctccggt gcgttcgggt cgtgcagccc cagtcgcccg ttcaccacgg
     3181 tctgccacgc atcgatgcgc ggacggtctg cccggtcgta gtacggctgg agacgcctgc
     3241 cggtcagcag ttccgtgttc ggtaccagaa aattcagttc cagccgcccc ttgtcccggt
     3301 gttcaaccca cagcacgctg tactggtctt tatcgagtcc gggcatcaga acccgctcga
     3361 agctcgccat cagcttttca cgctggccgg gcggtaaatc ctgttcagca aaagacagga
     3421 cgccggaggt gtactttttg gcgtagggcg aggcgtcgat aagctcccgg acctcctccg
     3481 gctttccctg caggacgatg gccccgtcgc gctggcggtc tttccccagc agataatcca
     3541 ccggcccggc accgccgcct cgccctcgcg gatgaaactt aacgatcatc gtctgccccc
     3601 ttttccagca cggcatgccg cagccgctcg agttcggcat caatggccat cagcgcggcc
     3661 accacctgaa cccggtcagc gcctgaccac tggccgccgt taattttccg ggcgatctgg
     3721 ttgaggttgt tccccatccc ggcaagctgg cgcagcaaaa ccggatcgat ggacggcagc
     3781 cgcaacgacc gcgccgggcg cgtatcgaga caggtttccc gcatccacac cgccagttgc
     3841 cttccattgc agcgctccag cagctgctgg tactccccat cggtcacccg gatacctaaa
     3901 aaacgggtac gcttaaccgc catcctctcc tccgatatcc gcaaaatgtg accgggctga
     3961 acgctcaagc tctgccatct gctcccggcg gtaatcttcc tcctgtgcct gacgcatcag
     4021 atttgccatc actgccggat gccaggatct tttttttacg acgagcggct tcttcttcgg
     4081 cttctgcatt atccctcctt cgcaaacgcc ggacggcacg gaaaagtttt ccgggtcgct
     4141 ccggcgttgg acaagcgcca gcgcgtcagt ggtgttagcg ggtttcgggg cgcagccctg
     4201 aaccagtcac agcgtttacg ctgtgtatac tggcttacta tgttggcatg agagcacgat
     4261 tcatgaaagt gcctcatgtg gcaggtgtga tatgtggctc aggagcgtcc gcaataccat
     4321 gcacggacag atgaaatgac gcctcctcgc tcactcggta gctgcgctcc tgccgttcgg
     4381 ctgcggcggg cgtgaccgac tcacggataa cgggataaag caaaggcgct caaatcagaa
     4441 agggccggag aaaaggatgc ggcgttgccg tttttccata ggctccgcct cccctgacga
     4501 gcatcacaga aatggacgct caagtcagag gaggacgaaa cccgacagga cttaaagatg
     4561 ccggtgattt ctctccggcg gctccctcgt gcgctctcct gttccgaccc tgccgtttac
     4621 cggatacctg ttccgccttt ttccctttcg ggaagcgtgg cgctttctca tagctcacgc
     4681 tgctggtatc tcggctcggt ttaggtcgtt cgctccaagc tgggctgtat gcacgaaccc
     4741 cccgttcagc ccgactgctg cgccctttcc ggtaactatc atcttgagtc caacccggac
     4801 ggacacgcaa aaacgccact ggcagcagcc attggtaact ggatatatac gagagagata
     4861 ttgagaggtc ttgaagtggg tgcctaactg cggctacact gaaagaacag ttttggtatc
     4921 tgcggtctcg tatacccagt tacccggtta agcagttccc caactgactt aaccttcgat
     4981 caaaccgcct ccccaggcgg ttttttcgtt tacagtgcaa gagattacgc gcagaaaaaa
     5041 aggatctcaa gaagatcctt taaatgatcg tcagttctgt tatccccgga gctccatcca
     5101 tcccacggaa aaacacccgc cactgatgcc ctcagcgcac tgcacatcat gtacaaacca
     5161 ctccctccct ccttttttat tgcgtttctt aatgcgcaat ggtagctttg tgcgcaacaa
     5221 aaaacgcaat agcaaggggg ggtaatgaga gaaagaacaa atatcatgct agatatagga
     5281 ttaaagcaat ccttacaacg gctttcgaat aaaaccaaaa tgagtctaag tgatcttatt
     5341 aaccagagat tatcaggttc actttcagtt gaagaacgca ttggcgaaaa gcctgaatgg
     5401 atgagaaacc ttgatcccta tctcgccaat cacttttcaa atattgagtt accgctccct
     5461 tcaccattaa aagaatacca agaaaaaatg gatgagatag attcaattat taatgacatg
     5521 atggttctaa aggaatattt cagagctaag ttttacgacg aaataaaata ggcacgctat
     5581 acggaggcgg agaatt
//
