LOCUS       NZ_CP138473             8334 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain
            KP96_L17_12.23_ST6796_NDM5 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP138473
VERSION     NZ_CP138473.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38201183
            Assembly: GCF_033802785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Klebsiella pneumoniae ST147 - the rise of a multiresistant and
            hypervirulent type
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-JUN-2023
            Assembly Method :: Flye v. 2.9.1-b1780; Medaka v. 1.7.2
            Assembly Name                     :: q20run14_BC10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 81.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 16:13:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,909
            CDSs (total)                      :: 5,786
            Genes (coding)                    :: 5,611
            CDSs (with protein)               :: 5,611
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 85 of 175
            Pseudo Genes (incomplete)         :: 93 of 175
            Pseudo Genes (internal stop)      :: 29 of 175
            Pseudo Genes (multiple problems)  :: 26 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138473.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP96_L17_12.23_ST6796_NDM5"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-03"
                     /genotype="ST6796"
     gene            join(8078..8334,1..376)
                     /locus_tag="SG220_RS26530"
                     /old_locus_tag="SG220_26525"
     CDS             join(8078..8334,1..376)
                     /locus_tag="SG220_RS26530"
                     /old_locus_tag="SG220_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            742..984
                     /locus_tag="SG220_RS26535"
                     /old_locus_tag="SG220_26530"
     CDS             742..984
                     /locus_tag="SG220_RS26535"
                     /old_locus_tag="SG220_26530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1330..1731)
                     /locus_tag="SG220_RS26540"
                     /old_locus_tag="SG220_26535"
     CDS             complement(1330..1731)
                     /locus_tag="SG220_RS26540"
                     /old_locus_tag="SG220_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1731..1922)
                     /locus_tag="SG220_RS26545"
                     /old_locus_tag="SG220_26540"
     CDS             complement(1731..1922)
                     /locus_tag="SG220_RS26545"
                     /old_locus_tag="SG220_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2338..2451
                     /locus_tag="SG220_RS26550"
                     /old_locus_tag="SG220_26545"
                     /pseudo=""
     CDS             2338..2451
                     /locus_tag="SG220_RS26550"
                     /old_locus_tag="SG220_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2796..3911
                     /locus_tag="SG220_RS26555"
                     /old_locus_tag="SG220_26550"
     CDS             2796..3911
                     /locus_tag="SG220_RS26555"
                     /old_locus_tag="SG220_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            3911..4543
                     /locus_tag="SG220_RS26560"
                     /old_locus_tag="SG220_26555"
     CDS             3911..4543
                     /locus_tag="SG220_RS26560"
                     /old_locus_tag="SG220_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            4909..5151
                     /locus_tag="SG220_RS26565"
                     /old_locus_tag="SG220_26560"
     CDS             4909..5151
                     /locus_tag="SG220_RS26565"
                     /old_locus_tag="SG220_26560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(5497..5898)
                     /locus_tag="SG220_RS26570"
                     /old_locus_tag="SG220_26565"
     CDS             complement(5497..5898)
                     /locus_tag="SG220_RS26570"
                     /old_locus_tag="SG220_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5898..6089)
                     /locus_tag="SG220_RS26575"
                     /old_locus_tag="SG220_26570"
     CDS             complement(5898..6089)
                     /locus_tag="SG220_RS26575"
                     /old_locus_tag="SG220_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6505..6618
                     /locus_tag="SG220_RS26580"
                     /old_locus_tag="SG220_26575"
                     /pseudo=""
     CDS             6505..6618
                     /locus_tag="SG220_RS26580"
                     /old_locus_tag="SG220_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            6963..8078
                     /locus_tag="SG220_RS26585"
                     /old_locus_tag="SG220_26580"
     CDS             6963..8078
                     /locus_tag="SG220_RS26585"
                     /old_locus_tag="SG220_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     Typing          2044..2451
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
     Typing          6211..6618
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 agaactacaa atctcctccc ccagctcgca taacgagata gctatgaatg gcaatacaaa
       61 aatttctttc tttataatgc caggaaaaga caagcaaggt gccattgaag atctgataat
      121 tgaacacgca agcactcgag cgttatatga atacataata gaatttttcg aaaaaattaa
      181 aaataataaa gatgacatct cagcgagtga caaagagtac acttatccaa caaacgaaaa
      241 aaaagctaag gtacaagtct atcttagctg caatagagaa tccgactcaa gaataggaat
      301 atccatgaaa aatggaataa taaacacagc agacaattgc tttgatgaaa taaaagattt
      361 tatttcaaaa atataaatcc aaatcaagcc agcccttcac gggctggctt atcaccaggg
      421 tgttattaaa cgccggataa cgtatagaaa gcatcataca ctttagttat cagcaatctc
      481 aaacgtccta aagacatcac cagatgccaa aacaatgatt gtgcagatct aatgtaaaag
      541 acccagcaca agatgctccc ttaagttata ttctctctat tcacttaaaa caatttaaca
      601 taaaatcata attgtacgca ccgctaaaac ggactcagga cgtttctgac gggaaaccgg
      661 taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg
      721 catgcatgag tatgcgcgca agtgcatagc gcggaaattc acaccattca gccaggggaa
      781 gtcctgtttc cggcgttctt ctcctccgaa aacggtgaac ggggacgcaa tttattgcgt
      841 ggccgtgaag cgttgccggc gcagccggaa aaccggtttt cagcaccttc aggaatcggg
      901 ttcgtcgacg gccggcaatc acgttatggc aagtcgccgg acgactcacc acacgattca
      961 ccggcgattc gaatcgtgaa ttgaatcaag agtcaccatg agcaaaaaac acccaaagtg
     1021 atgaattgaa tcaagagtcg attcatcata acaatatgat aataaagcgt gattcacata
     1081 tgaatcgtgg gcaattcgtc agcgattcgc catagagtcg tccacgactt ggatctgcac
     1141 tttgtaacac atcaccgcac atttattttt cattaaccca ttgttgtaaa taaggaatct
     1201 gcaaaatttt aattttgcgt ggggtgtggg tatttttagt ggtgaggagc gttcagagat
     1261 ctgacgattt ttgggaaggt tcaggaactc cctgggatgg ccggagagga aaactgaagc
     1321 atgacggggt caggattttt tccagaattt ccaccatggc gccggggatt cagttaattt
     1381 tgcctggtga ttttcaagaa ggagcatggc ctgtttcagg ctgtcaatgt gcgaatcctt
     1441 ggacaaaagt aaggtgttca ggtgttcaat ctcctgtttc agatgacgga tcacctgttc
     1501 actctggtca gcagaggctg tatcacgctg tccagagacc tgtccagaat ggtgttcacc
     1561 cttcggttgc tcaaaggggc caaagacacg taaaagctct gaagtatcta ccatttttgc
     1621 attgcgctca ccgctacagg tggacaggtc acctgcttta attaacctgt gcagtgttgt
     1681 tctactctta cccgtcagtc gggcagcctc actaatagac actctggcca tcagttacca
     1741 tcctgtaatg catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg
     1801 agtttctcac aaaggtcggc ctcgacatcg agatcaagct cgttaagctt ctccagcagg
     1861 agcagagact gattctggat gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc
     1921 ataagaggcc tcagagcgtc agtggtcagt gtagggatct cctcttttcc gcttcctcgc
     1981 tcactcagtc gctacgctcc tgccgttcgg ctgcggcgag cggcaggagc atgcgtagaa
     2041 acgctcgaga aaaccgagta atcgcatgga ggaaagaagg aaaggccgca cagcggccgt
     2101 ttttccatag gctccgcccc cctgacgagc atcacggaat ctgacgctca aatcagtggt
     2161 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctgatggc tccctcgtgc
     2221 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
     2281 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
     2341 cacgaacccc ccgttcagtc cgactgctgc gcctaatccg gtaactatcg tcttgagtcc
     2401 aacccggaaa gacacgacaa agcgccactg gcagcagcca ctggtaactg atttagggag
     2461 ttagtcttga agttatacgc cagttaaagc taaactaaac gaacagtttt ggtgactgcg
     2521 ctccctttaa gccagttacc tcggttcata gagcgccaac tctactgaac ctttcgaaaa
     2581 aaccgcccgc caaggcggtt tttttcgttc acagccggaa gcttacggct gctgccagat
     2641 aatctcaaga agaaagtaaa aaaaatcgat taaaacacaa gcaatcaaag ttaatctttg
     2701 acataaagtc tagctaatca caatgaaagc tgtacatttt attaaggtac taacaaatcg
     2761 tatggcatat aacaaaaaca cgaacagagg ctatcatgat aaaggaaata agtttaaaga
     2821 acttcaaatg cttcaatgag ctatatctaa agcaattaaa gactttaaat atcatagctg
     2881 ggaaaaacaa ctacgggaaa acatctatac tggatgctat tttttgtttt tatgatgtaa
     2941 aaaacccggc tgttttgtta aacattcaag cattcagaaa agaaatggca gaaataaaca
     3001 aaaacaagcc attttgggtt agttactttc atgatatgga cacatcacaa aagatgtcca
     3061 tagtcattcg agatgaacgc tctgaagtaa cacaaacata tgaaacagaa acaaatcaga
     3121 gattagaatc aagtttgtct ctaaacgttg acaccgtact atctaaccaa aacatacctc
     3181 gtcaaactat cggttctcag gccacagtaa actctttaaa aataaatgtg accgagacac
     3241 ccaatgcgaa aaacagggtt tccaataagc ttttctctat gcaattgaaa gaagatggcg
     3301 tagaaataaa atccgaaata aaaaacgaaa gggatgggat taattataaa tttaagacag
     3361 caacaataat aacaacatcc agaaagataa acaaagaggc aacaattaca aatgtatcta
     3421 gcctactaac acaaaaaagg aaaaaagaca tactagaaaa cttaaaaaaa atagatgaca
     3481 gaattgtaga tatagcaata tctgcgatag gtaacaataa ggaaatatat ttagacattg
     3541 gatttagtga gctaaatgaa atatcaatgc ttggcgaggg tatcagtcga gcattatctt
     3601 ttatcagctc agtgctggta caggaaaact caattattct tattgatgaa atcgagaacg
     3661 gaatacatta ctcagtaatt aaagacataa taaaatcatt aatttcatct gcaaaacaaa
     3721 acaacaatca aatatttgca acaactcata gtcaagatgt gatacgagca ataaacgaaa
     3781 tagatagcaa aaatgaagat atcgcttata ttagactagg aagagaaaag aactccctaa
     3841 aaccaacagc cgtacaattc aatatggatg atttctccta ctcagttgag aacgactggg
     3901 aggtaagata atgaaagaca taaataatag caaaaagatc attttcgttg agggtgatga
     3961 tgattttcat tttctatgca acctacttga aatacaaggc attaccgatg tttttgtcga
     4021 aaaaataaat ggaaaatcaa aattaaaaca agcacttaga gcattcaaaa acattgattc
     4081 tttcgatgaa aaagaaagta tttttattat attagatgct gacacatcat tttctgacac
     4141 agaaagatca ataattgcca cattgcgaga actacaaatc tcctccccca gctcgcataa
     4201 cgagatagct atgaatggca atacaaaaat ttctttcttt ataatgccag gaaaagacaa
     4261 gcaaggtgcc attgaagatc tgataattga acacgcaagc actcgagcgt tatatgaata
     4321 cataatagaa tttttcgaaa aaattaaaaa taataaagat gacatctcag cgagtgacaa
     4381 agagtacact tatccaacaa acgaaaaaaa agctaaggta caagtctatc ttagctgcaa
     4441 tagagaatcc gactcaagaa taggaatatc catgaaaaat ggaataataa acacagcaga
     4501 caattgcttt gatgaaataa aagattttat ttcaaaaata taaatccaaa tcaagccagc
     4561 ccttcacggg ctggcttatc accagggtgt tattaaacgc cggataacgt atagaaagca
     4621 tcatacactt tagttatcag caatctcaaa cgtcctaaag acatcaccag atgccaaaac
     4681 aatgattgtg cagatctaat gtaaaagacc cagcacaaga tgctccctta agttatattc
     4741 tctctattca cttaaaacaa tttaacataa aatcataatt gtacgcaccg ctaaaacgga
     4801 ctcaggacgt ttctgacggg aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc
     4861 agaaacgcgc aggctctgtt tttacggcat gcatgagtat gcgcgcaagt gcatagcgcg
     4921 gaaattcaca ccattcagcc aggggaagtc ctgtttccgg cgttcttctc ctccgaaaac
     4981 ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac
     5041 cggttttcag caccttcagg aatcgggttc gtcgacggcc ggcaatcacg ttatggcaag
     5101 tcgccggacg actcaccaca cgattcaccg gcgattcgaa tcgtgaattg aatcaagagt
     5161 caccatgagc aaaaaacacc caaagtgatg aattgaatca agagtcgatt catcataaca
     5221 atatgataat aaagcgtgat tcacatatga atcgtgggca attcgtcagc gattcgccat
     5281 agagtcgtcc acgacttgga tctgcacttt gtaacacatc accgcacatt tatttttcat
     5341 taacccattg ttgtaaataa ggaatctgca aaattttaat tttgcgtggg gtgtgggtat
     5401 ttttagtggt gaggagcgtt cagagatctg acgatttttg ggaaggttca ggaactccct
     5461 gggatggccg gagaggaaaa ctgaagcatg acggggtcag gattttttcc agaatttcca
     5521 ccatggcgcc ggggattcag ttaattttgc ctggtgattt tcaagaagga gcatggcctg
     5581 tttcaggctg tcaatgtgcg aatccttgga caaaagtaag gtgttcaggt gttcaatctc
     5641 ctgtttcaga tgacggatca cctgttcact ctggtcagca gaggctgtat cacgctgtcc
     5701 agagacctgt ccagaatggt gttcaccctt cggttgctca aaggggccaa agacacgtaa
     5761 aagctctgaa gtatctacca tttttgcatt gcgctcaccg ctacaggtgg acaggtcacc
     5821 tgctttaatt aacctgtgca gtgttgttct actcttaccc gtcagtcggg cagcctcact
     5881 aatagacact ctggccatca gttaccatcc tgtaatgcat ccagtctcga ggacagtgta
     5941 cggaaaagat gctccgcatc atcgtggagt ttctcacaaa ggtcggcctc gacatcgaga
     6001 tcaagctcgt taagcttctc cagcaggagc agagactgat tctggatgaa tttagccatg
     6061 ttcagcgctg ttttctgttg tttcgtcata agaggcctca gagcgtcagt ggtcagtgta
     6121 gggatctcct cttttccgct tcctcgctca ctcagtcgct acgctcctgc cgttcggctg
     6181 cggcgagcgg caggagcatg cgtagaaacg ctcgagaaaa ccgagtaatc gcatggagga
     6241 aagaaggaaa ggccgcacag cggccgtttt tccataggct ccgcccccct gacgagcatc
     6301 acggaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     6361 cgtttccccc tgatggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     6421 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
     6481 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc
     6541 taatccggta actatcgtct tgagtccaac ccggaaagac acgacaaagc gccactggca
     6601 gcagccactg gtaactgatt tagggagtta gtcttgaagt tatacgccag ttaaagctaa
     6661 actaaacgaa cagttttggt gactgcgctc cctttaagcc agttacctcg gttcatagag
     6721 cgccaactct actgaacctt tcgaaaaaac cgcccgccaa ggcggttttt ttcgttcaca
     6781 gccggaagct tacggctgct gccagataat ctcaagaaga aagtaaaaaa aatcgattaa
     6841 aacacaagca atcaaagtta atctttgaca taaagtctag ctaatcacaa tgaaagctgt
     6901 acattttatt aaggtactaa caaatcgtat ggcatataac aaaaacacga acagaggcta
     6961 tcatgataaa ggaaataagt ttaaagaact tcaaatgctt caatgagcta tatctaaagc
     7021 aattaaagac tttaaatatc atagctggga aaaacaacta cgggaaaaca tctatactgg
     7081 atgctatttt ttgtttttat gatgtaaaaa acccggctgt tttgttaaac attcaagcat
     7141 tcagaaaaga aatggcagaa ataaacaaaa acaagccatt ttgggttagt tactttcatg
     7201 atatggacac atcacaaaag atgtccatag tcattcgaga tgaacgctct gaagtaacac
     7261 aaacatatga aacagaaaca aatcagagat tagaatcaag tttgtctcta aacgttgaca
     7321 ccgtactatc taaccaaaac atacctcgtc aaactatcgg ttctcaggcc acagtaaact
     7381 ctttaaaaat aaatgtgacc gagacaccca atgcgaaaaa cagggtttcc aataagcttt
     7441 tctctatgca attgaaagaa gatggcgtag aaataaaatc cgaaataaaa aacgaaaggg
     7501 atgggattaa ttataaattt aagacagcaa caataataac aacatccaga aagataaaca
     7561 aagaggcaac aattacaaat gtatctagcc tactaacaca aaaaaggaaa aaagacatac
     7621 tagaaaactt aaaaaaaata gatgacagaa ttgtagatat agcaatatct gcgataggta
     7681 acaataagga aatatattta gacattggat ttagtgagct aaatgaaata tcaatgcttg
     7741 gcgagggtat cagtcgagca ttatctttta tcagctcagt gctggtacag gaaaactcaa
     7801 ttattcttat tgatgaaatc gagaacggaa tacattactc agtaattaaa gacataataa
     7861 aatcattaat ttcatctgca aaacaaaaca acaatcaaat atttgcaaca actcatagtc
     7921 aagatgtgat acgagcaata aacgaaatag atagcaaaaa tgaagatatc gcttatatta
     7981 gactaggaag agaaaagaac tccctaaaac caacagccgt acaattcaat atggatgatt
     8041 tctcctactc agttgagaac gactgggagg taagataatg aaagacataa ataatagcaa
     8101 aaagatcatt ttcgttgagg gtgatgatga ttttcatttt ctatgcaacc tacttgaaat
     8161 acaaggcatt accgatgttt ttgtcgaaaa aataaatgga aaatcaaaat taaaacaagc
     8221 acttagagca ttcaaaaaca ttgattcttt cgatgaaaaa gaaagtattt ttattatatt
     8281 agatgctgac acatcatttt ctgacacaga aagatcaata attgccacat tgcg
//
