LOCUS       NZ_CP138471             2967 bp    DNA     circular CON 26-APR-2024
DEFINITION  Klebsiella pneumoniae strain 22-MO00052 plasmid p22MO52E, complete
            sequence.
ACCESSION   NZ_CP138471
VERSION     NZ_CP138471.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38201489
            Assembly: GCF_038447555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Hammerl,J.A. and Binsker,U.
  TITLE     Genetic basis of colistin-resistant Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Hammerl,J.A. and Binsker,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2023) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Aug 2023
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 45.0x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038447555.1-RS_2024_04_25
            Annotation Date                   :: 04/25/2024 17:15:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,409
            CDSs (total)                      :: 5,283
            Genes (coding)                    :: 5,165
            CDSs (with protein)               :: 5,165
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 52 of 118
            Pseudo Genes (incomplete)         :: 67 of 118
            Pseudo Genes (internal stop)      :: 22 of 118
            Pseudo Genes (multiple problems)  :: 18 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138471.1.
            Bacteria and source DNA available from Dr. Jens A. Hammerl, German
            Federal Institute for Risk Assessment, Berlin, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="22-MO00052"
                     /isolation_source="Poultry meat"
                     /db_xref="taxon:573"
                     /plasmid="p22MO52E"
                     /geo_loc_name="Germany"
                     /collection_date="2022"
     gene            complement(687..1166)
                     /locus_tag="SF665_RS27045"
                     /old_locus_tag="SF665_27050"
     CDS             complement(687..1166)
                     /locus_tag="SF665_RS27045"
                     /old_locus_tag="SF665_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000275450.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERIR
                     ELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLESKNA
                     LVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          177..437
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.275"
                     /coverage="100.0"
                     /evalue="1.83e-109"
ORIGIN
        1 atgtgcaaat gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagggctt
       61 cgccccgaca ccccgcatca gtgcgtacgt gctcaatgtg gtttccggtt ctcttccgct
      121 tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc
      181 tcgcatgttg tcggtgattc cgctgttgtt ctttcgggga taagtcgtca gatagccctt
      241 caaacgcgct cagaagctct cagacgcacg aaacggggtt taaatgcccg tcagggcatg
      301 gaaggcgcga aaaacgctta cagagcgttt caggaggtca ggcgcgccga tacccctagc
      361 caggggcttt caggtcgcca ggaaccgcgc cagccccctg caaagcccat ttgcacggta
      421 caggcagcca ctcggttggt aggtggtcgt agaccgtcgg tgttacagcg atgggggtct
      481 ggggttctcc ccagacggtt tccggttgcg ctcaaggcgc tgccggaacc gtggcggctc
      541 ggtcgttctg gagatcgggg agcaaaaaca cgcaagggat tttgaggcgg caaaagagca
      601 atgcaggata gacaacccga tgtcaaatgt taacatttga atcagattgt ccaaaaacgg
      661 ggtttttgcc tgtgcctgcc gctggctcaa tttgaaaaca tggccatgat tcggcgctga
      721 atatcaccgt ctttgttgac cagtgtttta gtgacaaacg ccgcgtagtt tttagatgtg
      781 ttgtatccct tccagacttc atgtggatcg agtgcatacg cacaaacctt accagcaaca
      841 ggaattctga ctaacgcgtt ttttgattct aagtagttga gccagcgctt aattgtgctt
      901 cggctaacac ccaatttatc tgctaaaaac tgctgatcgg caacaacagc accgcatgag
      961 ggatctatgt gctcagctaa aaacgagtaa agcggtattg ctacaactcc ctgcttgtcc
     1021 ttcgacagtt ctcggatcct ttcccagccc ttctgataaa cctgggtgaa tgggccgtta
     1081 tttttcggat ctgattccga ttctttgttt aatttttcca gttcttttag acgctttttt
     1141 cgctgctctt cagtcccgat atacatggta aatctccacg gtctaatgat ggctcaaata
     1201 taagccatca tagctcaaac gtaaaccatg ataggtcgac ctagctcaca catgcgtgag
     1261 ctaaccttag accacacatg cgtgagctaa cccataatgc aagtagctaa aaaatcaata
     1321 acttacaaaa tcgcccttaa atatcttttc tgctgaaagt gttgcgcaaa gcggaacact
     1381 tttttggggt gtcggggcga agccctgacc cagtcacgta gcgatagcgg agtgtatact
     1441 ggcttatccc gtcaccgtca gttcgccgat cttgcggaac ggctgcgaca cgaaaacggc
     1501 aaaatgattt gctgtccggc tgacagaatt ttgcacggtg agggcggcag cggagccaaa
     1561 aatcatccgc ctgaaaagtg cggatgcgca cttagacgtt tctctacttc gtcgagaaac
     1621 tgtgcgctcc tgcggagggc ttaacggcgc agaactgccg atgctctgcc agtgttcagg
     1681 cgaaaaaatc gggacaagat acatctttgt attaccgcca cacggtcggt actccaaaca
     1741 tcctggtcag ggctgcaccc cgacacccgc caacgcctca cggcacgcga aaaaattccg
     1801 cgtaccgttc accgttttct ctcatgaaaa cgtgtcgttt tgaccagttc gacggtcgga
     1861 gcgcgcgaaa ccgatcacgc atctgtcttg tgcatagcta tgcaacgcgc ttaaaacgtt
     1921 tgtgacgcgt ttttgtgggt gtggacgggg aaaaacacgg tttttggact ttttgaacgt
     1981 aaagacgtga aaaaaacgtg ttattgcact tgcgatcttt cgtcggggag cgtagaacga
     2041 aaaaagcccg ccattaggcg agcttcttcg ggttcattga ggttttcgac ctcctcaatg
     2101 ttcctgtgga gtctgttgcg gtgacaacgg aaaccagttc attcagtttg catgaacctt
     2161 ctgtattctt caagcattac cgaccgcaca gcctccccag aatcccgaaa aaatcctgtt
     2221 tttgcagatg attgcgtgtt tgcaaagacc gtttgcggtt gggggagctg tgacgacggc
     2281 aaccgcacgc gtgagagttc cgctaccggc gcagagagtt tcatcgacag cggacacctg
     2341 atgagttgcc acaaaaaccg tatgtagcgc ggctagattg gttgagttag accttggcgg
     2401 cagttcccgg tatgtggtgg cgtgacgggt gagatggtgc ggcgcagacg catacgtcac
     2461 gcaggcgcgg gttcttccac tgacgcgccc cgtgatcgat ggccctggct ccgtccggca
     2521 tatcgagaac ggcaaactat ccgtcccttc gctgggaggg gtgagttgtt gccgttccct
     2581 cagaccctcc accatccagc atatcgcacg gaccccaaca gcactgcaac aacagacaac
     2641 tccagagcaa aaaaaaaaac agaactgccg gacaatatgc gagagatcac accgctcgcc
     2701 gcagccccag cgaccgaacg tagtgagcga gtgggcgagg aagcggaaga gaaccggaag
     2761 tcacattgag cgcgtacgca ctgatgcggg gtgtcggggc gaagccctga cccagtcaca
     2821 gagcgatagc ggagtgtata ctggcttact cctctgcaag ccttaccagc tgtgattttt
     2881 gccagatttc acgctctcaa cgttcaacag ctccatcatg gttttgccgt ccgttcaccg
     2941 ctaaaacgcc tcagaaatcg ggacagg
//
