LOCUS       NZ_CP138470             3631 bp    DNA     circular CON 26-APR-2024
DEFINITION  Klebsiella pneumoniae strain 22-MO00052 plasmid p22MO52D, complete
            sequence.
ACCESSION   NZ_CP138470
VERSION     NZ_CP138470.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38201489
            Assembly: GCF_038447555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Hammerl,J.A. and Binsker,U.
  TITLE     Genetic basis of colistin-resistant Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Hammerl,J.A. and Binsker,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2023) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Aug 2023
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 45.0x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038447555.1-RS_2024_04_25
            Annotation Date                   :: 04/25/2024 17:15:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,409
            CDSs (total)                      :: 5,283
            Genes (coding)                    :: 5,165
            CDSs (with protein)               :: 5,165
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 52 of 118
            Pseudo Genes (incomplete)         :: 67 of 118
            Pseudo Genes (internal stop)      :: 22 of 118
            Pseudo Genes (multiple problems)  :: 18 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138470.1.
            Bacteria and source DNA available from Dr. Jens A. Hammerl, German
            Federal Institute for Risk Assessment, Berlin, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="22-MO00052"
                     /isolation_source="Poultry meat"
                     /db_xref="taxon:573"
                     /plasmid="p22MO52D"
                     /geo_loc_name="Germany"
                     /collection_date="2022"
     gene            complement(368..1297)
                     /locus_tag="SF665_RS27025"
                     /old_locus_tag="SF665_27030"
     CDS             complement(368..1297)
                     /locus_tag="SF665_RS27025"
                     /old_locus_tag="SF665_27030"
                     /inference="COORDINATES: protein motif:HMM:NF027286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="WP_023307555.1"
                     /translation="MKEKIKKFGLPWICLFIVYFVCFLVVAATIIRHVINTTEEITLTT
                     YITPIFGLASLFVAMVTIRYNAVTNKEKNALDFVKEFKEKEIHDAMVEIYKTRRKYKNK
                     TNNRLNRKDISPYCEKAAAISQDDIKVIIPAEKKLELAEQIDLLTEEMKYTSSPSAKSK
                     IRGKISSLQKKLNDSNELEQICKFRSALIKVLNLMERCANGVRYGLYDEGLIYNSYGTQ
                     FIEIYELAFSFIKRRQDDEDKLFINAEWLAVKWTLQKEIQEDKPTFMTNQKRAIQHANE
                     GLKSHLKAPDKSKLEPLLNNLQKLKFPR"
                     /name="DUF4760 domain-containing protein"
     gene            complement(1773..1886)
                     /locus_tag="SF665_RS27030"
                     /old_locus_tag="SF665_27035"
     CDS             complement(1773..1886)
                     /locus_tag="SF665_RS27030"
                     /old_locus_tag="SF665_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071787400.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAAIGN"
                     /name="replication initiation protein"
     gene            2291..2482
                     /locus_tag="SF665_RS27035"
                     /old_locus_tag="SF665_27040"
     CDS             2291..2482
                     /locus_tag="SF665_RS27035"
                     /old_locus_tag="SF665_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_011977837.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2482..2883
                     /locus_tag="SF665_RS27040"
                     /old_locus_tag="SF665_27045"
     CDS             2482..2883
                     /locus_tag="SF665_RS27040"
                     /old_locus_tag="SF665_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064154307.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKDEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHPAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1774..2150)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="92.0"
                     /evalue="7.61e-175"
ORIGIN
        1 tagaaataac agaggctcac tggagaacct ctgttgaaac ttatctttta tcattcttag
       61 caggtgcact catgcggtct gtttccgcat ttttcacagt cattacaacg cttacagaca
      121 taatccacct ctttctgatt acactctctg cagtagctgc aagtgcaagc gctaccaggt
      181 aagccgcgtc cgtcacaaat tttgtccatt ggatccatat ctacatcctt tgtgtggctt
      241 ttttagcgtg caatttcagt gatcacaact gacgagttcg taaggacgga ttgatgagag
      301 ggaaacccct gtatttcagg ggtttacatt ccatataatg caaattacgc ggcctgataa
      361 aaacttttta tcgcgggaat ttcagttttt gtaagttgtt aagcaatggt tctaatttgc
      421 ttttatctgg tgctttcagg tgtgatttca gcccttcgtt agcatgttgt atagctctct
      481 tttgattagt cataaatgtt ggcttgtctt cctgaatctc tttttggaga gtccatttta
      541 ctgctaacca ttcagcattt atgaataact tatcttcatc atcctgcctt ctttttatga
      601 atgagaaggc gagctcatat atttcaatga actgggttcc atatgagtta tatataagac
      661 cttcatcgta tagaccatat ctcacaccat ttgcacatcg ctccattaaa tttaacactt
      721 taattagagc tgatctgaat ttgcaaattt gttctaattc attgctatca ttgagttttt
      781 tttggagtga ggatattttt cctctgattt ttgattttgc gcttggggat gaagtgtatt
      841 tcatctcctc agtgagtaga tctatttgct cagcaagttc tagttttttt tctgcgggta
      901 tgattacttt tatatcatcc tgagatatgg cggcagcttt ttcgcagtaa ggtgagatgt
      961 cttttctgtt taatctattg tttgttttgt ttttatactt tcttcttgtt ttatatattt
     1021 caaccattgc atcatgtatt tctttttctt tgaattcttt tacgaaatcc aatgcatttt
     1081 tttctttatt ggtaacagca ttgtatcgta tggttaccat agcaacaaat aagctggcga
     1141 gtccaaatat tggcgttata tatgtggtta atgttatctc ttcggttgta tttattacat
     1201 gcctgataat agtggctgcg actactaaaa aacatacaaa gtagacaata aaaaggcata
     1261 tccaagggag gccaaatttt tttatttttt ctttcatgtg tatgccgcta tgtgggttat
     1321 agcggcgttt tactatcaga aaatgcctaa gtcaatctct ctaacgattt gggtcatttt
     1381 ttcgacaacg ttaagcttct gcataggatt acctctcaat cgaggtggta atgattatca
     1441 ttatcacctc tacataatgt agagccccat atgtacatgg gtttcctcgg caagtaaatg
     1501 cctacaagtt taagtttagt taaaaaatgt gagttcgatt agtcattaag ggtaaattca
     1561 ccatgaaaga tcttcttgag atcctctgac gatcatcgta atcttttggc atacaaacga
     1621 aaaaaccacc cggcaaggtg gtttttcgag ggttcagtaa accggcaagt ttctgaaccg
     1681 aggtaactgg ctagtagagg agcacagtca ccaaaactgt tcgtttagtt tagccttaac
     1741 cggcgtataa cttcaagact atctcctcta aatcagttac caatggctgc tgccagtggc
     1801 gcttttgcat gtcttaccgg gttggactca agatgatagt taccggataa gacgcagcag
     1861 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     1921 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga taccggtaaa
     1981 ccgaatggca ggaacaggag agcgcacgag ggagccatca gggggaaacg cctggtatct
     2041 ttatagtcct gtcgggtttc gccaccactg atttgagcgt caaattctgt gatgcttgtc
     2101 aggggggcgg agcctatgga aaaacggccc ctgcgcggcc ttctccctct ccgtgctgcc
     2161 tgtcctatgt cgttatgtgc gcgttgctac cgctcgccgc agccgaacgg caggagcgta
     2221 gcgactgagt gagcgaggaa gcggaaaaga gactggtttt acactgagca ctgacgctct
     2281 gaggcttctt atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagca
     2341 tcagtctcta ctcctgctgg agaagcttaa cgagcttgat cttgatgctg aggccgacct
     2401 ttgtgagaaa ctccacgatg atgcggagca tcttttccgt acactgtcct cgagactgga
     2461 tgcattacag gatggtaact gatggccaga gtgtctatta gtgaggctgc ccgactgacg
     2521 ggtaagagta gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc
     2581 ggtgaacgca atgcaaaaat ggtagatact tcagagcttt tacgtgtctt tggtcccttt
     2641 gaacaaccga aggatgaaca ccattctgga caggtctctg aacagcgtga tacagcctct
     2701 gctgaccaga gtgaacaggt gattcgtcat ctgaaacagg agattgaaca cctgaacact
     2761 ttacttttgt ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa
     2821 aatcacccgg caaaattaac tgaatccccg acgccatggt ggaaattctg gaaaaaatcc
     2881 tgaccccgtc atgcttcagt tttcctctcc ggccatcccg gggagttcct gaaccttccc
     2941 aaaaatcgtc agatctctga acgctcctca ccactaaaaa tacccacacc ccacgcaaaa
     3001 ttaaaatttt gaagataact cattaatatc agttagttgt gggttattaa attacgcagt
     3061 attactttct gtaaggtgaa tccaagtcgt cggcgattcg tgtacgactt gccgacgact
     3121 cgccatagag tcatcgacga ctcactttag actatcagta acttatgacg acttgactct
     3181 agagtcgaat cgtcattttg gtgttttttt gaccttggcg actctagagt caagtcacga
     3241 gtcgaatcgc cggtgaatcg tgtggtgagt cgtccggcga cttgccataa cgtgattgcc
     3301 ggccgtcaac gaacccgatt cctgatcgtg ctgaaaaccg gttttccggc tgcgccggca
     3361 acgcttcacg gccacgcaat aaattgcgtc cccgttcacc gttttcggat aggaagaacg
     3421 tcggaaacag gacttaccct ggctgaatgg cgtgaatttt cgcgctatgc acttgcgtgc
     3481 atactcatgc atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt
     3541 gttgcctgtt ttaccggttt cccgtcagaa acgccctgag gccgttttag cggtgcgtac
     3601 aatcagggct ttatgttaaa tactctcgca c
//
