LOCUS       NZ_CP138469             5631 bp    DNA     circular CON 26-APR-2024
DEFINITION  Klebsiella pneumoniae strain 22-MO00052 plasmid p22MO52C, complete
            sequence.
ACCESSION   NZ_CP138469
VERSION     NZ_CP138469.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38201489
            Assembly: GCF_038447555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Hammerl,J.A. and Binsker,U.
  TITLE     Genetic basis of colistin-resistant Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Hammerl,J.A. and Binsker,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2023) Biological Safety, German Federal Institute
            for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Aug 2023
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 45.0x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038447555.1-RS_2024_04_25
            Annotation Date                   :: 04/25/2024 17:15:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,409
            CDSs (total)                      :: 5,283
            Genes (coding)                    :: 5,165
            CDSs (with protein)               :: 5,165
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 52 of 118
            Pseudo Genes (incomplete)         :: 67 of 118
            Pseudo Genes (internal stop)      :: 22 of 118
            Pseudo Genes (multiple problems)  :: 18 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138469.1.
            Bacteria and source DNA available from Dr. Jens A. Hammerl, German
            Federal Institute for Risk Assessment, Berlin, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="22-MO00052"
                     /isolation_source="Poultry meat"
                     /db_xref="taxon:573"
                     /plasmid="p22MO52C"
                     /geo_loc_name="Germany"
                     /collection_date="2022"
     gene            complement(join(5575..5631,1..177))
                     /locus_tag="SF665_RS26990"
                     /old_locus_tag="SF665_26995"
     CDS             complement(join(5575..5631,1..177))
                     /locus_tag="SF665_RS26990"
                     /old_locus_tag="SF665_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(184..714)
                     /locus_tag="SF665_RS26995"
                     /old_locus_tag="SF665_27000"
     CDS             complement(184..714)
                     /locus_tag="SF665_RS26995"
                     /old_locus_tag="SF665_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<752..1450)
                     /locus_tag="SF665_RS27000"
                     /old_locus_tag="SF665_27005"
                     /pseudo=""
     CDS             complement(<752..1450)
                     /locus_tag="SF665_RS27000"
                     /old_locus_tag="SF665_27005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1392..1715)
                     /locus_tag="SF665_RS27005"
                     /old_locus_tag="SF665_27010"
     CDS             complement(1392..1715)
                     /locus_tag="SF665_RS27005"
                     /old_locus_tag="SF665_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1780..1971
                     /locus_tag="SF665_RS27010"
                     /old_locus_tag="SF665_27015"
     CDS             1780..1971
                     /locus_tag="SF665_RS27010"
                     /old_locus_tag="SF665_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3174..4040)
                     /locus_tag="SF665_RS27015"
                     /old_locus_tag="SF665_27020"
     CDS             complement(3174..4040)
                     /locus_tag="SF665_RS27015"
                     /old_locus_tag="SF665_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(4042..5094)
                     /locus_tag="SF665_RS27020"
                     /old_locus_tag="SF665_27025"
     CDS             complement(4042..5094)
                     /locus_tag="SF665_RS27020"
                     /old_locus_tag="SF665_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     Typing          complement(2066..2147)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(2604..2791)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(3..1402)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgcacctgc tgactcaact gcatgactcg cccgttcagc atcgcgttgt cccgttgcgt
       61 aagcgaaaac attttctgca attccacgaa ggcgttctcc cattcgttca gccgctgcat
      121 atagtcctgt tgcagttgct ctaatgcgtt cagcaaatgt ctttccagct ccgtcatgct
      181 ctgtcactcc tgcaccagtc tcactgcgtt cagtttgccg ttgtccttcg tccagttcgt
      241 ttcggctttc atccccttcg gcaacaccag aaaacgtccg ttgctgtcct cgtgataacg
      301 cacgccccat gtcttcgcgt tcagcttctc cagactctct ttctgctcgc ggagaatatt
      361 catattcgcg tttatcttca tgccctggta ccacagcaga ctcccggtcg tggcaaacat
      421 cagtactgag acgataatca gccacagcca gcgtttcacg ctcattttca gtacggtttt
      481 ccagtgcgcg tccatcgtat cgacggtcgt tttctgatac ctctccagca gttctttcag
      541 ggctgttgta tggtcgttga tagcgttcct gattaaagat tcgtttgagg tcagttccct
      601 gtttacagat tcttcgagct tcctgaactc gtcgttcagc attttctctg tagattcggc
      661 tctctctttc agctttttct cgaactccgc gcccgtctgt aaaagattgc tcataaaacg
      721 ctcctttcag tctgatattc ctcccgccgt tcgggtctgc aatgctgata ctgcttcgcg
      781 tcaccctgac cacttcaagc cccgcctctg tgagcgcctg aatcacatcc tgacggcctt
      841 ttatctctcc ggcatggtaa agcgcgtcta tcccgcgtgt gacgccctca gcaagcgcct
      901 gtttcgtttc cggcaggtta tcaggaaggg tcagcgtccg gcggttctcc ggggcgttgg
      961 ggtcatgcag accgtaatgg tgattcacca gcgtctgcca ggcatcaatt ctcggcctgt
     1021 ctgcccggtc gtagtacggc tggaggcgtt ttccggtctg tagctccatg ttcggaatga
     1081 caaaattcag ctcaagccgt cccttgtcct ggtgctccac ccacaggatg ctgtactgat
     1141 ttttctcgag accgggcatc agtacacgct caaagctcgc catcactttt tcacgccctc
     1201 caggcggcag ctccttctcc gcgaacgaca gaacacctga cgtgtatttc ttcgcaaatg
     1261 gcgtggcatc gatgagttcc cggacctctt ccggattccc ctgaagcacc gttgcccctt
     1321 cccggttacg ctcccttccc agcaggtaat caaccggacc actgccacca ccttttcccc
     1381 tggcatgaaa cttaactatc atcccgcgcc ccctgttccc tgaccgccag acgcagccgg
     1441 cgcagctcat ccccgatggc catcagtgcg gccaccacct gaacccggtc accggaagac
     1501 cactgcccgc tgttcacctt cctcgctgtc tggttaaggt tattcccgat ggcggccagc
     1561 tgacgcagta acggcggtgc cagtgtcggc agcttcccgg aacgggcaac cggctcaccc
     1621 aggcagaccc gccgcatcca taccgccagt tgtttaccct cacagcgttc cagtaaccgg
     1681 gcatgttcat catcagtaac ccgtatcgtg agcatcctct cgcgtttcat cggtatcatt
     1741 acccccatga acagaacccc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc
     1801 gcccttaaca tggcccgctt catcagaggc cagacattaa cgctgctgga gaagctcaac
     1861 gaactggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgccgatgag
     1921 ctttaccgca gttgcctcgc acgcttcggg gatgacggtg aaaaccgctg acacatgcag
     1981 ctcccggaga cggtcacagc ttgtctgtga gcggatgccg ggagcagaca agcccgccag
     2041 ggcgcgtcag gccgttgacc tttggggtgt cggggcgaag ccctgaccca gtcacgtagc
     2101 gatagcggag tgtatactgg cttaaccatt cggcatcagt gcggattgta tgaaaagtgc
     2161 gccatgtccg gtgtgaaatg ccgcacagat gcgtaaggag aaaatgccag ttcaggcgct
     2221 cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcggctgcgg cgagcggact
     2281 ctcctttctc aaaaacgcta accctgttat ccacagaacc cggggataaa ccaggaaaga
     2341 acatgtgcgc aaaaaacgaa accccgggaa aggccgcgcc ggaggcgctt tttccatagg
     2401 ctccgccccc ctgacggact tcacaaaaca gacacttttt cggtgcatct gtgggagcct
     2461 tgcggctcaa ccatgaaatc cgccagcggt ggcgaaaccc gacgtggctt aaagatcatc
     2521 accgtttccg gtgaatcgct ccctcctgcg cccccacgtt cccgccatat cgattaccgg
     2581 ataccttttc cgcctttctc ccttacggga cacgtggcgc tttctcatag ctcacgtctt
     2641 ctggtatctc ggggcgactt aggcagttcg ctccgggctg ggctgtatgc aggaaccccc
     2701 cgttcagccc gactgctgcg ccttctccgg taactgttct cttgggtcca acccggaaaa
     2761 gacaccacaa agcgccactg gcagcagcca ttggtaaaag tcatgtgcga gatatttaga
     2821 gagtacttga agtggctgcc ggagtgcggc tacactagaa gaacaatttt ggtgactcgt
     2881 ctcgcataag acagttacct ggttaagcag tctgcaagct gacttaacct tcgatcaaac
     2941 cgccctgcaa ggcggttttt tcgtttacag gcagcagatt acgcgcagaa aaaaaggatc
     3001 tcaagaggat cctttgatct ttttgttaca aacgtctcta tttgactccc ctgagagcgt
     3061 tttagcgcgc ctctgacccc cttggttaca ttccgacacc agaaacgctc ccgtttttta
     3121 cgtgcatttg gcacggtatc gatggaggcc ggggggcaat cctgaacaga atgttactcc
     3181 tctgagtatt ttctgttaag aaaatataca cttagcacag ttacaataaa tcctaccccc
     3241 tgtaagaatg tgaattgttc atccaaaaac aaccatgaca aaaataatgc aactagcgga
     3301 ataaagtaca acattatccc tgccaaatga actgggagcc aacgcaagga gttcaagtaa
     3361 aaacaaaaag ctaaaaaata ttgtaatatt ccagatgaaa aaacaataat cacggattcc
     3421 aacggaagat aaattgatga tttagatata aaaataaaga gcgatgacag tataagggag
     3481 aatgtcaact gtccgctcaa taatgtgata ggctcaaacg atttaataag gcgagacgat
     3541 ataacaacat aaaatgctgc cgataacaca ccggcaacaa caagacaata tccaacaata
     3601 ttattcgatc cattactgat gtctggcaag gctaccaaaa tactgccaac aattgcacct
     3661 ataaggaaca atataaaata ttgcggggaa ttaattttaa ctgagcaaat aagaacagac
     3721 aacaccaata taaacactga ttcaagtgaa aaaataattg atgcgcttcc tgctgaaata
     3781 aaataaagtc cgtagagtgt aaaagtatac gctaagaatg gttcaaaaac gcctgtcgca
     3841 taagcaagta tcgcttttga gtttaatggg agtttttttc tttttataaa aagaactatc
     3901 caagatagta ttactgcact acaaatctgc cagaaaaaca cccctccggc atctagttct
     3961 tcaactcctt ttttgctcat tacgatagct gccccccatg agaacgctgc taaaaaagca
     4021 aaaagcattc ctttaatcat attaatcatc ctctttctca taaaggggtt tgttttcaaa
     4081 tttccttggt tgaaaggagt taaaaaacat agatgcatca tctacaggaa taccaataaa
     4141 ctctccataa taaggatcat ctataggaaa ctcttctaat gaaacataaa acaatacatt
     4201 ccagttgaag atcattggat cactattttc tatttcatgg agcaatcttt catccttagt
     4261 taattctcct ggaattaata catcagtatg ataaaaaccg ctgtaataag aactattacc
     4321 ggtgtgcact gggcatccaa aacgatccaa attagattct gaatgccatg ttgtagaaat
     4381 cccttccgga tgtgaatacc cacccttacc aacagactga atagtcatgt tacgaatatc
     4441 cgctaacgct tgttcttgat ctttatagaa acctttttct atcgccagcc tcataaaatg
     4501 ccccggggca tttacagaaa taagtattcc ccctggtatt cttctatcat gatttcttgt
     4561 tctatgagca gaagagtaaa aaacattaac accagcctta tatattttta gcccatcttt
     4621 atcctgaaat ggtacgcttt cagtataaat aacatcctct tttattggat agcactctgg
     4681 catatgcagc gaacaaatat atttttgtat gtccactaac tcttctgatg gacgagcaaa
     4741 ggcaagttgt ggatgaataa aataaatgag atgcgcactg acctctcctc ttcttgcgcg
     4801 ttctttaaaa cgaactcttt cattacgcaa aaaatcaaac aaatgaccat catcattata
     4861 tagttggggg ctatttacaa tagataaatg gaaatcaagc cctttaattt tcttgcttgc
     4921 caatttacca aatacacagg gctgattgcc tgacacccat tttcttagta aatcttcaat
     4981 tttatcttta gaagtttcgt taaagataaa tgaatgaata ttatcaatat cttttccaaa
     5041 tgaaaaactg gatgataatt gtggtagttt atttatacgt tgttgaagat tcatgaaagc
     5101 tcccacaaga cacctcatat atttttttat atactgaaac tacattatat ttattaccgg
     5161 cgtaaaacga aataatttat tatcgttacc gatattttaa aaaaatcatt atttgcacag
     5221 tgttcgagtt gttagtatca ctccttcctg aaaatccgct ccatttacca taacgtcgca
     5281 ttgtacgcac cgccaaaacg cgttcggtgc gatcacggca gcagacaggt aaaaatggca
     5341 acaaaccacc cgaaaaactg ccgcgatcgc acctgataaa ttttaaccgc atgaatacct
     5401 atgcaaccag atgctacagg ccacattagc ccgccttaat ccactgaaca cgtcattttt
     5461 catggtttca ccatcccagc gaagggccat ccagtgtccg ctcctggatt tccggctgac
     5521 gctcccgctc cagagataac tcatgttcgc gttcctgcat cagccgttca tcccttatct
     5581 cctgttctcg ctgtataact ggctcaagcg ttctgtccgc tcgctcaagt g
//
