LOCUS       NZ_CP138439             3465 bp    DNA     circular CON 20-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 64EVAM plasmid p64EVAM-8, complete
            sequence.
ACCESSION   NZ_CP138439
VERSION     NZ_CP138439.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38124869
            Assembly: GCF_033802585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Tet(X4) producing Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) Henning Seedorf Group, Temasek Life Sciences
            Laboratory Limited, 1 Research Link National University of Singapore
            Singapore, Singapore 117604, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9; Nanopolish v. 0.13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 499x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/20/2023 01:48:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,825
            CDSs (total)                      :: 5,697
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 93 of 187
            Pseudo Genes (incomplete)         :: 104 of 187
            Pseudo Genes (internal stop)      :: 24 of 187
            Pseudo Genes (multiple problems)  :: 30 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138439.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="64EVAM"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p64EVAM-8"
                     /geo_loc_name="Singapore"
                     /collection_date="2019-01"
     gene            50..259
                     /locus_tag="SE701_RS29100"
     CDS             50..259
                     /locus_tag="SE701_RS29100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319161993.1"
                     /translation="MSELVRDHLGRVSVRNKDVDRERIAMLNRINANLNMIARWVNTHK
                     SAASSVEVVAHLMDIERHIRELSR"
                     /name="hypothetical protein"
     gene            complement(346..756)
                     /locus_tag="SE701_RS29105"
     CDS             complement(346..756)
                     /locus_tag="SE701_RS29105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232693406.1"
                     /translation="MVRLSDLTCAPWVIRVSQAIPGVDFTAQVIESLFCGISGRADVQA
                     FPGAKLNAGRHKMQLVMPGMGMPDPEDVVLIGFKPGERDLFKAIHELLLHFRRNLLLRG
                     ERQHAGSVFVPEIERVDKVAGSLRGSSQYFRR"
                     /name="hypothetical protein"
     gene            992..1495
                     /locus_tag="SE701_RS29110"
     CDS             992..1495
                     /locus_tag="SE701_RS29110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_232693405.1"
                     /translation="MSVSLTLQERLKQQHLAESETIQSLTKQQLSSLRSELNGIFQQEL
                     NTMLEDMRRQLHDTTEDMRQSRQSMLLTVVKNRVIFPALSAVTILAVIFLSGWGLSTFQ
                     ESRIMANWQEINSQKATLSALESKTAGLNIVQDKNGTFVVLPKGAKVDDSWTVGKQNAL
                     RIVR"
                     /name="mobilization protein"
     gene            1499..1732
                     /locus_tag="SE701_RS29115"
     CDS             1499..1732
                     /locus_tag="SE701_RS29115"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_232693404.1"
                     /translation="MTELEQHLLSALKRMEQQFSEQLRASEKAQAALRSMFERTAEDNA
                     TLLKQVSFLDNRVTVLSEQLKQFGNLYRQNRR"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            complement(1920..2426)
                     /locus_tag="SE701_RS29120"
     CDS             complement(1920..2426)
                     /locus_tag="SE701_RS29120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232693408.1"
                     /translation="MSTYNLLNLRKFIFKVLMQAFCFFSFLFISLNVKAESLSGKLEYQ
                     NVSGMIYKKLSSCQDVAIDNYSYTDCKFTAEKDFDVYLGFLRSSIENNLKNSNPLLAKF
                     DSDVKCDSLINDRLSNNIGSMWVGISADLCVVMKDNALKKWMLFYEAMKYSDQYNKKLD
                     GIFDK"
                     /name="hypothetical protein"
     gene            complement(2426..2752)
                     /locus_tag="SE701_RS29125"
     CDS             complement(2426..2752)
                     /locus_tag="SE701_RS29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232693407.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLADLDYENYIQVAQIDIAEAL
                     RMQKTHVSRAVKNLIEFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALANGLSVIQ
                     GGRS"
                     /name="hypothetical protein"
     Typing          257..1827
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgcgaattt gaggcgctga aacagctggc cgaagatgca ggttgcacta tgtcagagct
       61 ggttcgcgat catctcggca gggtcagcgt gcgcaacaaa gacgtcgatc gggagcggat
      121 cgccatgctt aaccgcatca acgccaacct gaacatgatt gcccgctggg ttaatacgca
      181 taaatcggcg gcttcctcgg ttgaggtcgt tgctcacctg atggatatcg agcgtcatat
      241 tcgggagctg tcgcgatgat tgtgggtttc agcaggcacg gtactggcgg cggcggtggc
      301 ccggtcggct atgtcaccga taaaacgcga cccggtcggg aaaactcacc gccggaagta
      361 ctgagaggat ccccggaggc tacccgcaac cttatcgact cgctcgattt ccggcacaaa
      421 tacacttccg gcgtgctgtc tttcgccccg gaggagaagg ttacgccgga aatggagcag
      481 gagttcatgg atcgctttga acaggtcgcg ttcgccgggc ttgaagccga tcagtacaac
      541 atcctctggg tcaggcatac ccatgccggg catcacgagc tgcattttgt gacgccccgc
      601 gttgagcttg gcaccgggaa aagcctgaac atccgcccgc ccggagatgc cgcaaaacag
      661 gctttcgatg acctgcgcag tgaaatcaac gccaggtatg gcctggctga cccggatgac
      721 ccacggcgcg cacgtgagat cgctcagcct gaccatgagc tgaaaatcgc cgcagaagcc
      781 ctcagaaggg gcgagaagcc gcaggaagac atcaggctac tcatagacgc ggttttatct
      841 cagagggccg cacaggggct tatacgcagt cgtagcgacg ttatcgagca ggtttcagac
      901 cttggaatgc aggtaacgcg cgaaggtaaa aattacatca ccgtgtcaga tccgcagagc
      961 ggccagcggt ggcgcatgaa agggggactt tatgagcgtg agtttgacgc tgcaggagcg
     1021 gttgaagcag cagcatctcg cagagagcga gactattcaa agcctgacga agcagcagct
     1081 gagcagtttg cgaagcgagt tgaacggcat atttcagcaa gagctgaata ccatgctgga
     1141 agatatgcgc cggcagctgc acgacacgac agaggatatg cgccagagcc ggcagagcat
     1201 gttgctgacc gtcgtgaaaa accgggtgat attcccggcc ctgagtgcgg ttacgatctt
     1261 ggccgttatc tttctgagcg gctggggcct gagtacgttt caggaaagcc ggatcatggc
     1321 caactggcag gagatcaaca gccagaaagc taccttatcg gcgctggaga gcaaaacagc
     1381 gggtttgaac atcgtccagg acaagaacgg gacgttcgtc gttctgccca aaggggcgaa
     1441 agtggacgac agctggacag tcgggaagca aaacgccctg cggatcgtga ggtagactat
     1501 gacggaactg gaacagcact tgttgagcgc cttgaagcgt atggagcagc aattcagcga
     1561 gcaactcagg gcatccgaga aggcgcaggc cgccttgcgg agcatgttcg agcgtactgc
     1621 ggaagacaac gccacgctgt tgaagcaagt cagcttctta gacaatcggg tgactgtatt
     1681 gtcggagcag ctgaagcagt tcgggaacct ctacaggcag aacaggcgct gaggaggcag
     1741 ctggagtttc aggaggcgtt acaacggagc cagcaggagc cagagcctcc gacgctgaag
     1801 tcaaacagat attatgggcc gagtatgtag gacttacagt aaagccctcg tttaaaacga
     1861 aggctttacc ttatggaagt tgctaacgaa aacaatggaa cttaatttat taaaataaat
     1921 tacttatcaa aaatgccatc tagtttctta ttgtattgat cagagtattt cattgcttca
     1981 taaaaaagca tccatttttt taaagcatta tccttcatca caacacacaa atcagctgaa
     2041 atacctaccc acatgctccc aatattgtta cttagtctat catttattaa tgaatcgcat
     2101 ttaacatcag agtcaaattt agccaaaaga ggattgctgt tttttaaatt attttcgatt
     2161 gatgatctga gaaaacccaa gtacacatcg aaatcttttt ctgctgtaaa cttacaatca
     2221 gtataagaat aattatcaat agctacatct tggcaagagg ataatttttt ataaatcata
     2281 ccactaacgt tctggtattc aagctttcca gataaagatt cggcttttac atttagtgaa
     2341 ataaataaga aagaaaaaaa acaaaacgct tgcattaaaa ctttaaagat gaacttcctc
     2401 aaatttaata aattataagt agacattaag atcggccacc ttggataaca ctaagaccat
     2461 ttgcaagagc ttttttatga ttgctcaccg tacctttcca gccaaactga ggatttagac
     2521 gataagtttt gctgcggcca atctttggcc cctcgagaat aattccaaac tcaattaggt
     2581 tcttaactgc ccgacttacg tgggtctttt gcatgcgcag tgcttcggca atatctatct
     2641 gagctacttg tatgtaattc tcataatcga gatcagctaa gagagccatt aacacgcgca
     2701 tttgatcgtg atttagctca ttggcgattg taatcagtgc agcttgattc atagtaaaat
     2761 gcctctgaaa tgacgacttc tgcttaggtc ggactactgc aacaaatcct cctagatcct
     2821 ctcctgtttc gaggtctatc tgtgtgactc tccgcatctg aatctgccct gatagttacc
     2881 atgtgaggta accaagttac catgtgaggt aactatctgc aaaaactttc ctttgctggc
     2941 acggcttgcg ggacatccct ttctgtatct tgttactccc tctgcactaa tcacagttta
     3001 tgtcataccg agtgaacgca tttttatgtc cgttcagatg atgttccgct tcctcgttcc
     3061 ggctgttcgt tcctcgctgc gctgcggtac gcctcagccg gaactgcggc gagcggtacc
     3121 ggctcattca aaaagcgatc tcgctgcctg gtttcgtcaa gggcgagcgg agcgagtgca
     3181 tttcaccctt gacgactcac cagataaaac acgcttgtca ggggtggatg gtttcacgca
     3241 gtgaggccat ccacctgaca gacgccgttg ccctgggaga gggagcagag cgaccgctgc
     3301 aagggagggg tcaaggggag gcgaagccgc ccattgcaag gcccgatgtg cgacattgag
     3361 cgtagcgaaa tgtagcaaca tcgggtagcc ttgtttaacg tgcaatatgc gacgacggag
     3421 atgaaatgga aaagcgcgat aacatgctgc gggtcaggtt ttctg
//
