LOCUS       NZ_CP138435            13489 bp    DNA     circular CON 20-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 64EVAM plasmid p64EVAM-4, complete
            sequence.
ACCESSION   NZ_CP138435
VERSION     NZ_CP138435.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38124869
            Assembly: GCF_033802585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Tet(X4) producing Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13489)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) Henning Seedorf Group, Temasek Life Sciences
            Laboratory Limited, 1 Research Link National University of Singapore
            Singapore, Singapore 117604, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9; Nanopolish v. 0.13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 499x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/20/2023 01:48:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,825
            CDSs (total)                      :: 5,697
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 93 of 187
            Pseudo Genes (incomplete)         :: 104 of 187
            Pseudo Genes (internal stop)      :: 24 of 187
            Pseudo Genes (multiple problems)  :: 30 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138435.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="64EVAM"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p64EVAM-4"
                     /geo_loc_name="Singapore"
                     /collection_date="2019-01"
     gene            complement(join(13368..13489,1..706))
                     /locus_tag="SE701_RS27455"
     CDS             complement(join(13368..13489,1..706))
                     /locus_tag="SE701_RS27455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="WP_319161652.1"
                     /translation="MSYLVKEVDSKDVFFFKKVLRKKNKSRVIYSVDDKSSSIYKDMEK
                     SISKRFICEISKKENFLIKKVPYAYYPGRSANQNAKAHQPFDFTLSMDIRDHFTSITPK
                     MVDWLLTPRELYLCFIDGSLPQGLSTSPVISNIAMMEVDRAILSELSNVVSNGSEPING
                     FVYRIKNFRYTRYSDDLVVSFNLHEPSMHLHRRIFLYIEALISKTIRQFGFEPNNNKVK
                     MQNEKSGFRVITGAREIIKPTRKTKRKLRSAIHNANIDNAIGLYNWIKLVSRT"
                     /name="reverse transcriptase domain-containing protein"
     gene            complement(703..999)
                     /locus_tag="SE701_RS27460"
     CDS             complement(703..999)
                     /locus_tag="SE701_RS27460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071010377.1"
                     /translation="MDRSEFEKLISDLESKLKRAVLRVGLKKIEVQVFLLFPLGESQLG
                     TIHVEAYRRTDILNRKKPQPIYKIKSDCFKDIERGFLKWVIDYKSTIDKVAIK"
                     /name="hypothetical protein"
     gene            1709..1900
                     /locus_tag="SE701_RS27465"
     CDS             1709..1900
                     /locus_tag="SE701_RS27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2522..2632
                     /locus_tag="SE701_RS27470"
     CDS             2522..2632
                     /locus_tag="SE701_RS27470"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_259425790.1"
                     /translation="MDWISLAIAIALLVVAVYTGISYYKEKKTISRAFRR"
                     /name="small membrane protein"
     gene            complement(2842..3078)
                     /locus_tag="SE701_RS27475"
     CDS             complement(2842..3078)
                     /locus_tag="SE701_RS27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     gene            3774..3887
                     /locus_tag="SE701_RS27480"
     CDS             3774..3887
                     /locus_tag="SE701_RS27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(4372..5043)
                     /locus_tag="SE701_RS27485"
     CDS             complement(4372..5043)
                     /locus_tag="SE701_RS27485"
                     /inference="COORDINATES: protein motif:HMM:NF012307.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="WP_319161656.1"
                     /translation="MKKENFLIKKVPYAYYPGRSANQNAKAHQPFDFTLSMDIRDHFTS
                     ITPKMVDWLLTPRELYLCFIDGSLPQGLSTSPVISNIAMMEVDRAILSELSNVVSNGSE
                     PINGFVYRIKNFRYTRYSDDLVVSFNLHEPSMHLHRRIFLYIEALISKTIRQFGFEPNN
                     NKVKMQNEKSGFRVITGVSVSREIIKPTRKTKRKLRSAIHNANIDNAIGLYNWIKLVSR
                     T"
                     /name="reverse transcriptase domain-containing protein"
     gene            complement(5254..5499)
                     /locus_tag="SE701_RS27490"
     CDS             complement(5254..5499)
                     /locus_tag="SE701_RS27490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319161654.1"
                     /translation="MDRSEFEKLISDLESKLKRAVLRVGLKKIEVQVFLLFPLGESQLG
                     TIHVEAYRRTDILNRKKPQPIYKIKSDCFKDIERGF"
                     /name="hypothetical protein"
     gene            6207..6398
                     /locus_tag="SE701_RS27495"
     CDS             6207..6398
                     /locus_tag="SE701_RS27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7018..7128
                     /locus_tag="SE701_RS27500"
     CDS             7018..7128
                     /locus_tag="SE701_RS27500"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_259425790.1"
                     /translation="MDWISLAIAIALLVVAVYTGISYYKEKKTISRAFRR"
                     /name="small membrane protein"
     gene            complement(7338..7574)
                     /locus_tag="SE701_RS27505"
     CDS             complement(7338..7574)
                     /locus_tag="SE701_RS27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     gene            8270..8383
                     /locus_tag="SE701_RS27510"
     CDS             8270..8383
                     /locus_tag="SE701_RS27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(9161..9700)
                     /locus_tag="SE701_RS27515"
     CDS             complement(9161..9700)
                     /locus_tag="SE701_RS27515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319161648.1"
                     /translation="MSYLVKEVDSKDVFFFKKVLRKKNKSRVIYSVDDKSSSIYKDMEK
                     SISKRFICEISKKENFLIKKVPYAYYPGRSANQNAKAHQPFDFTLSMDIRDHFTSITPK
                     MVDWLLTPRELYLCFIDGSLPQGLSTSPVISNIAMMEVDRAILSELSNVVSNGSEPING
                     FVYRIKTSDTQGILMI"
                     /name="hypothetical protein"
     gene            10704..10895
                     /locus_tag="SE701_RS27520"
     CDS             10704..10895
                     /locus_tag="SE701_RS27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            11520..11630
                     /locus_tag="SE701_RS27525"
     CDS             11520..11630
                     /locus_tag="SE701_RS27525"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_259425790.1"
                     /translation="MDWISLAIAIALLVVAVYTGISYYKEKKTISRAFRR"
                     /name="small membrane protein"
     gene            complement(11838..12074)
                     /locus_tag="SE701_RS27530"
     CDS             complement(11838..12074)
                     /locus_tag="SE701_RS27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_319161650.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTRSRPRAPRR"
                     /name="hypothetical protein"
     gene            <12781..12885
                     /locus_tag="SE701_RS27535"
                     /pseudo=""
     CDS             <12781..12885
                     /locus_tag="SE701_RS27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(3242..3414)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.356"
                     /coverage="100.0"
                     /evalue="4.47e-49"
     Typing          complement(7738..7910)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.356"
                     /coverage="100.0"
                     /evalue="4.47e-49"
     Typing          complement(12239..12411)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.356"
                     /coverage="100.0"
                     /evalue="4.47e-49"
     Typing          complement(1398..1834)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.871"
                     /coverage="99.0"
                     /evalue="1.2499999999999998e-134"
     Typing          3339..3883
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.364"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(5896..6332)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.033"
                     /coverage="99.0"
                     /evalue="2.68e-136"
     Typing          7835..8379
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.364"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(10391..10829)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.492"
                     /coverage="99.0"
                     /evalue="7.500000000000001e-132"
     Typing          12336..12881
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.196"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 caccagtgat aactctaaag ccagactttt cattttgcat tttaacttta ttgttatttg
       61 gttcaaaacc aaactgcctt atggttttac ttattagggc ctctatatat aaaaatatac
      121 gcctatgtaa atgcatagat ggctcgtgca aattaaagct tacaactaaa tcatcagaat
      181 accttgtgta tcggaagttt tttattctgt atacaaaacc gtttattggt tcggagccgt
      241 tagatacaac atttgataat tcagataata tcgctctatc aacctccatc atggctatat
      301 tagaaattac tgggctagta gagagcccct gaggtaatga accatctata aaacataggt
      361 ataactccct tggggtaagt aaccaatcta ccatcttagg cgttatcgat gtgaagtgat
      421 ctcggatatc catagataat gtaaaatcga atggctgatg agcttttgcg ttttgattag
      481 ccgaccttcc cggatagtag gcatatggta ctttttttat gaggaagttt tctttttttg
      541 atatctcaca aataaatctt tttgatattg atttttccat atctttataa atagaagaag
      601 acttgtcgtc aaccgaatat atcactcttg atttgttttt cttccttaaa accttcttga
      661 agaaaaacac atccttagag tcaacctcct ttactaaata actcatttaa tagccacctt
      721 atcgatagtc gatttataat caatgaccca tttcaaaaat cccctttcga tatccttgaa
      781 acaatcactt tttattttat atattggctg tggtttttta cgattcagta tatctgtacg
      841 tctatatgct tcgacatgaa tagtacctag ttgcgattca ccgagtggga atagtaggaa
      901 tacttgaact tctatttttt tcaggccaac cctcaggact gcccgtttca gttttgactc
      961 cagatctgat attagttttt caaattcaga tctatccata cgccctccct aacctaaaat
     1021 catattggga taatttaaca taaggtcaca ttgcccgcac cgcctcaggc gccctcagag
     1081 cgtttctacg atctcacctg caaaagaaca caccctaccc cctgaaaacc gcccaggatc
     1141 gctcctgtaa ttcacaggcc atgaattgtt atacccccaa ctgactgacc ggcagttccg
     1201 ccactggaaa ccggtgaaat cgccaaaagt ggtggtgatc gggtcacaga aacggtgaac
     1261 gggcgaataa atttttttat tcgtctgtga ggcgttcgcg gggtgtcggg ggtcggccct
     1321 gacgggtttt aactaaaaaa ttttgcggat cagaaaatat tttaagggcg gaatgacacc
     1381 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccatcagggg gaaacgcctg
     1441 gtatctttaa gtcctgtcgg gtttcgccgc cactgatttg agcgtcagat ttcgtgatgc
     1501 ttgtcagggg gcggagccta tggaaaaacg ccccagcggg gcgtggcctt ttcgcccttg
     1561 tccttagctt tcgttatgct taccgttacg ttatctgctt atcctccctc cgctcgccgc
     1621 agactcatga ccgaacggag tgagcgaatg agcgaggaag cggaaaacca tcagaacgta
     1681 cagtaagcac tttcgcgccg gggccttcat gaacaaacaa caaaagacag ccgttaacat
     1741 ggctaaattc atccaggatc agtccctgct tcttctggac aggcttaatg agctggatct
     1801 ggatcatgag gctgatttct gcgagaaact ccatgaggat gcggaacgcc tttaccgaag
     1861 cctgtcagcg cgcctgaatg agcagcagga cggtgcgtaa tggctccgtt gtgggtatta
     1921 ccggagtaat accggcatcg ccttaatttt cattgtcttg gcgcttaata ttattacccc
     1981 ggtaataccc agaatccctt tattttttct ttttatttat ttttcatgaa gttgcgatga
     2041 aaatcgcagc tgcgttaggt gtatgaccat ttaatgatta aaaatcatca tttttggaag
     2101 ggcgctctcc gtaggttaag ggttatttgg ctaaaaagcg tcctagttct ttgatggtca
     2161 tgcaagcatg accatctggg caaccaaaaa ctacagataa actacagata aactacaaaa
     2221 acgttttgca ttagtgatgg cagactacaa aaaactacaa gaaaactaca gataaactac
     2281 aaagtaactg acctgctccc cgtgatttgt tacataccca ccacagggca ttattttttt
     2341 ttgatgatat gtacaaaatc agtgattagg aataatctta gccgccttaa caaacacata
     2401 caaaaacagc ggttatgttt catctcacca caataagtaa cgacagtatc atgctaaaaa
     2461 aagatgatat tatctttatg aaatcataac atgttgtttt tattaaatta atcaggcatt
     2521 tatggactgg atttcacttg ctatcgccat tgctctactt gttgtagctg tctacacagg
     2581 aatctcatac tacaaagaaa aaaaaacaat cagtcgtgca tttaggcgat agcgccctat
     2641 ttaccataat cccttcttac gcgcaccgca gaaacggcct cagggcgttt ctgcgccttc
     2701 agccacaaaa gagcacgtct tccccccaga aaaccgcccg gaatcgctct ggtgagtttt
     2761 acgggggtga atgactatgc accgtagtga ataccacgct gtttcgccct gaactcccca
     2821 cgaaaccgac gaaagtggca atcagcgacg cggcgcccgc ggacgcgacc aggtaaattc
     2881 cgcctgctgc cgctcctcct cacgcgcaga tcgctgccgt tctgcctggc gctgcgattc
     2941 gatctgtgca cgagccctgt taaggacaat gtgcctgttg ggctgattca gcttcttgcc
     3001 tgacgctctc tcagcggcgg caacagcatc gtcgagaaat cttttctcgg cgggcgtcag
     3061 gtttatcagt cgtttcatgg cgatctccgg tcagaataac tctgcacagt ctgccagaca
     3121 cggcgaacct catgaagaac ctgcagccgg gaacagccct gagatatcgt tttggtgccg
     3181 gcatcgtgat ggcggtactc caaaaacaca tcttgtcccg ctttcagttt ctgaatttgc
     3241 gggttccggg cgcagcgccg gaccagtcac gtagcgctag cggagtgtat actgccttag
     3301 tatgttggca tgagtgcgat aatcaggaaa gcgccttatg tggcaggaga aaaatgctgc
     3361 agcagagcgt aagcagaata tgcgatacag aagatattcc gcttcctcgc tcactgactc
     3421 gcttcgctcg gtcgttcggc tgcggcgagc ggtacagact tacggaaggg cggagatttc
     3481 ctggaagatg ccaagaggat acttaacagg gaagcgatag ggccgcggcg aagccgtttt
     3541 tccataggct ccgcccccct gacaagcatc acaaaatctg acgctcaaat cagtggcggc
     3601 gaaacccgac aggacttaaa gataccaggc gtttccccct ggtcgctccc tcgtgcgctc
     3661 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttatctca
     3721 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     3781 aaccccccgt tcagtccgac tgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3841 cggtaagaca tgcaaaagcg ccactggcag cagccactgg taactgattt agaggagata
     3901 gtcttgaagt catgcgccag ttaaggctaa actaaacgaa cagttttggt gactgcgctc
     3961 ctctaactag ccagttacct cggttcaaag agttgctgct ctgagaacct tcgagaaaac
     4021 cgccttgctg ggcggttttt tcgttttcag agcaagagat tacgacgaga ctgaatgaat
     4081 ctcaagattt catttagcct gccacccgat cattcaaagt tttggcgtag ttctgacccg
     4141 ctccccgtga tttttacata cccaccacgg gggcaataag gtttctattg cgttttttcg
     4201 ataaaacccg taggttttag aggtgattag ctgagaggtt ccgctccaca agagagatat
     4261 agcgagatga ttcgtccgct gaataatcct aacatctagt aacttagctt acgctaaacc
     4321 atggcccaca gggcaatgta tggactagaa cgtaatcaca cgtcgagaac attatgttct
     4381 cgacactaat ttgatccagt tatacagccc gatagcattg tctatgttgg cgttatgtat
     4441 tgctgatcta agttttcttt ttgttttcct tgttggcttt atgatttctc ttgagacgga
     4501 tacaccagtg ataactctaa agccagactt ttcattttgc attttaactt tattgttatt
     4561 tggttcaaaa ccaaactgcc ttatggtttt acttattagg gcctctatat ataaaaatat
     4621 acgcctatgt aaatgcatag atggctcgtg caaattaaag cttacaacta aatcatcaga
     4681 ataccttgtg tatcggaagt tttttattct gtatacaaaa ccgtttattg gttcggagcc
     4741 gttagataca acatttgata attcagataa tatcgctcta tcaacctcca tcatggctat
     4801 attagaaatt actgggctag tagagagccc ctgaggtaat gaaccatcta taaaacatag
     4861 gtataactcc cttggggtaa gtaaccaatc taccatctta ggcgttatcg atgtgaagtg
     4921 atctcggata tccatagata atgtaaaatc gaatggctga tgagcttttg cgttttgatt
     4981 agccgacctt cccggatagt aggcatatgg tacttttttt atgaggaagt tttctttttt
     5041 gatatctcac aaataaatct ttttgatatt gatttttcca tatctttata aatagaagaa
     5101 gacttgtcgt caaccgaata tatcactctt gatttgtttt tcttccttaa aaccttcttg
     5161 aagaaaaaca catccttaga gtcaacctcc tttactaaat aactcattta atagccacct
     5221 tatcgatagt cgatttataa tcaatgaccc atttcaaaat cccctttcga tatccttgaa
     5281 acaatcactt tttattttat atattggctg tggtttttta cgattcagta tatctgtacg
     5341 tctatatgct tcgacatgaa tagtacctag ttgcgattca ccgagtggga atagtaggaa
     5401 tacttgaact tctatttttt tcaggccaac cctcaggact gcccgtttca gttttgactc
     5461 cagatctgat attagttttt caaattcaga tctatccata cgccctccct aacctaaaat
     5521 catattggga taatttaaca taaggtcaca ttgcccgcac cgcctcaggc gccctcagag
     5581 cgtttctacg atctcacctg caaaagaaca caccctaccc cctgaaaacc gcccaggatc
     5641 gctcctgtaa ttcacaggcc atgaattgtt atacccccaa ctgactgacc ggcagttccg
     5701 ccactggaaa ccggtgaaat cgccaaaagt ggtggtgatc gggtcacaga aacggtgaac
     5761 gggcgaataa attttttatt cgtctgtgag gcgttcgcgg ggtgtcgggg gtcggccctg
     5821 acgggtttta actaaaaatt ttgcggatca gaaaatattt taagggcgga atgacaccgg
     5881 taaaccgaaa ggcaggaaca ggaaagcgca agagggagcc accgggggga aacgcccggt
     5941 atctttaagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt
     6001 gtcagggggg cggagcctat ggaaaaacgc ccctcggggc gtggcctttt cgcccttgtc
     6061 cttagctttc gttatgctta ccgttacgtt atctgcttat cttccctccg ctcgccgcag
     6121 actcatgacc gaacggagtg agcgaatgag cgaggaagcg gaaaaccatc agaacgtaca
     6181 gtaagcactt tcgcgccggg gccttcatga acaaacaaca aaagactgcc gttaacatgg
     6241 ctaaattcat ccaggatcag tccctgcttc ttctggacag gcttaatgag ctggatctgg
     6301 atcatgaggc tgatttctgc gagaaactcc atgaggatgc ggaacgcctt taccgaagcc
     6361 tgtcagcgcg cctgaatgag cagcaggacg gtgcgtaatg tctccgttct gagtattacc
     6421 ggagtaatac cggcatcgcc ttaattttca ttgtcttagc gcttaacatt attaccccgg
     6481 taatacccag aatccctgta ttttttattt tttatttatt tttcatgaag ttgcgatgaa
     6541 aatcgcagct gcgttaggtg tatgaccatt taatgattaa aaatcatcat ttttggaagg
     6601 gcgctctccg taggttaagg gttatttggc taaaaagcgt cctgttcttt gatggtcatg
     6661 caagcatgac catctgggca accaaaaact acagataaac tacagataaa ctacaaaaac
     6721 gttttgcatt agtgatggca gactacaaaa aactacaaga aaactacaga taaactacaa
     6781 agtaactgac ctgctccccg tgatttgtta catacccacc acagggcatt atttttttga
     6841 tgatatgtac aaaatcagtg attaggaata atcttagccg ccttaacaaa cacatacaaa
     6901 aacagcggtt atgtttcatc tcaccacaat aagtaacgac agtatcatgc taaaaaaaga
     6961 tgatattatc tttatgaaat cataacatgt tgtttttatt aaattaatca ggcatttatg
     7021 gactggattt cacttgctat cgccattgct ctacttgttg tagctgtcta cacaggaatc
     7081 tcatactaca aagaaaaaaa aacaatcagt cgtgcattta ggcgatagcg ccctatttac
     7141 cataatccct tcttacgcgc accgcagaaa cggcctcagg gcgtttctgc gccttcagcc
     7201 acaaaagagc acgtcttccc cccagaaaac cgcccggaat cgctctggtg agttttacgg
     7261 gggtgaatga ctatgcaccg tagtgaatac cacgctgttt cgccctgaac tccccacgaa
     7321 accgacgaaa gtggcaatca gcgacgcggc gcccgcggac gcgaccaggt aaattccgcc
     7381 tgctgccgct cctcctcacg cgcagatcgc tgccgttctg cctggcgctg cgattcgatc
     7441 tgtgcacgag ccctgttaag gacaatgtgc ctgttgggct gattcagctt cttgcctgac
     7501 gctctctcag cggcggcaac agcatcgtcg agaaatcttt tctcggcggg cgtcaggttt
     7561 atcagtcgtt tcatggcgat ctccggtcag aataactctg cacagtctgc cagacacggc
     7621 gaacctcatg aagaacctgc agccgggaac agccctgaga tatcgttttg gtgccggcat
     7681 cgtgatggcg gtactccaaa aacacatctt gtcccgcttt cagtttctga atttgcgggt
     7741 tccgggcgca gcgccggacc agtcacgtag cgctagcgga gtgtatactg ccttagtatg
     7801 ttggcatgag tgcgataatc aggaaagcgc cttatgtggc aggagaaaaa tgctgcagca
     7861 gagcgtaagc agaatatgcg atacagaaga tattccgctt cctcgctcac tgactcgctt
     7921 cgctcggtcg ttcggctgcg gcgagcggta cagacttacg gaagggcgga gatttcctgg
     7981 aagatgccaa gaggatactt aacagggaag cgatagggcc gcggcgaagc cgtttttcca
     8041 taggctccgc ccccctgaca agcatcacaa aatctgacgc tcaaatcagt ggcggcgaaa
     8101 cccgacagga cttaaagata ccaggcgttt ccccctggtc gctccctcgt gcgctctcct
     8161 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt atctcattcc
     8221 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     8281 ccccgttcag tccgactgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8341 aagacatgca aaagcgccac tggcagcagc cactggtaac tgatttagag gagatagtct
     8401 tgaagtcatg cgccagttaa ggctaaacta aacgaacagt tttggtgact gcgctcctct
     8461 aactagccag ttacctcggt tcaagagttg ctgctctgag aaccttcgag aaaaccgcct
     8521 tgctgggcgg tttttcgttt tcagagcaag agattacgac gagactgaat gaatctcaag
     8581 atttcattta gcctgccacc cgatcattca aagttttggc gtagttctga cccgctcccc
     8641 gtgattttta catacccacc acgggggcaa taaggtttct attgcgtttt tcgataaaac
     8701 ccgtaggttt tagaggtgat tagctgagag gttccgctcc acaagagaga tatagcgaga
     8761 tgattcgtcc gctgaataat cctaacatct agtaacttag cttacgctaa accatggccc
     8821 acagggcaat gtatggacta gaacgtaatc acacgtcgag aacattatgt tctcgacact
     8881 aatttgatcc agttatacag cccgatagca ttgtctatgt tggcgttatg tattgctgat
     8941 ctaagttttc tttttgtttt ccttgttggc tttatgattt ctcttgagac ggatacacca
     9001 gtgataactc taaagccaga cttttcattt tgcattttaa ctttattgtt atttggttca
     9061 aaaccaaact gccttatggt tttacttatt agggcctcta tatataaaaa tatacgccta
     9121 tgtaaatgca tagatggctc gtgcaaatta aagcttacaa ctaaatcatc agaatacctt
     9181 gtgtatcgga agtttttatt ctgtatacaa aaccgtttat tggttcggag ccgttagata
     9241 caacatttga taattcagat aatatcgctc tatcaacctc catcatggct atattagaaa
     9301 ttactgggct agtagagagc ccctgaggta atgaaccatc tataaaacat aggtataact
     9361 cccttggggt aagtaaccaa tctaccatct taggcgttat cgatgtgaag tgatctcgga
     9421 tatccataga taatgtaaaa tcgaatggct gatgagcttt tgcgttttga ttagccgacc
     9481 ttcccggata gtaggcatat ggtacttttt ttatgaggaa gttttctttt tttgatatct
     9541 cacaaataaa tctttttgat attgattttt ccatatcttt ataaatagaa gaagacttgt
     9601 cgtcaaccga atatatcact cttgatttgt ttttcttcct taaaaccttc ttgaagaaaa
     9661 acacatcctt agagtcaacc tcctttacta aataactcat ttaatagcca ccttatcgat
     9721 agtcgattta taatcaatga cccatttcaa aaatcccctt tcgatatcct tgaaacaatc
     9781 actttttatt ttatatattg gctgtggttt tttacgattc agtatatctg tacgtctata
     9841 tgcttcgaca tgaatagtac ctagttgcga ttcaccgagt gggaatagta ggaatacttg
     9901 aacttctatt tttttcaggc caaccctcag gactgcccgt ttcagttttg actccagatc
     9961 tgatattagt tttcaaattc agatctatcc atacgccctc cctaacctaa aatcatattg
    10021 ggataattta acataaggtc acattgcccg caccgcctca ggcgccctca gagcgtttct
    10081 acgatctcac ctgcaaaaga acacacccta ccccctgaaa accgcccagg atcgctcctg
    10141 taattcacag gccatgaatt gttatacccc caactgactg accggcagtt ccgccactgg
    10201 aaaccggtga aatcgccaaa agtggtggtg atcgggtcac agaaacggtg aacgggcgaa
    10261 taaatttttt tattcgtctg tgaggcgttc gcggggtgtc gggggtcggc cctgacgggt
    10321 tttaactaaa aaattttgcg gatcagaaaa tattttaagg gcggaatgac accggtaaac
    10381 cgaaaggcag gaacaggaga gcgcacgagg gagccatcag gggggaaacg cctggtatct
    10441 ttaagtcctg tcgggtttcg ccgccactga tttgagcgtc agatttcgtg atgcttgtca
    10501 gggggcggag cctatggaaa aacgccccag cggggcgtgg ccttttcgcc cttgtcctta
    10561 gctttcgtta tgcttaccgt tacgttatct gcttatcctc cctccgctcg ccgcagactc
    10621 atgaccgaac ggaagtgagc gaatgagcga ggaagcggaa aaccatcaga acgtacagta
    10681 agcactttcg cgccggggcc ttcatgaaca aacaacaaaa gacagccgtt aacatggcta
    10741 aattcatcca ggatcagtcc ctgcttcttc tggacaggct taatgagctg gatctggatc
    10801 atgaggctga tttctgcgag aaactccatg aggatgcgga acgcctttac cgaagcctgt
    10861 cagcgcgcct gaatgagcag caggacggtg cgtaatggct ccgttgtggg tattaccgga
    10921 gtaataccgg catcgcctta attttcattg tcttggcgct taatattatt accccggtaa
    10981 tacccagaat ccctttattt tttctttttt atttattttt catgaagttg cgatgaaaat
    11041 cgcagctgcg ttaggtgtat gaccatttaa tgattaaaaa atcatcattt ttggaagggc
    11101 gctctccgta ggttaagggt tatttggcta aaaagcgtcc tgttctttga tggtcatgca
    11161 agcatgacca tctgggcaac caaaaactac agataaacta cagataaact acaaaaaacg
    11221 ttttgcatta gtgatggcag actacaaaaa aactacaaga aaactacaga taaactacaa
    11281 agtaactgac ctgctccccg tgatttgtta catacccacc acagggcatt attttttttt
    11341 gatgatatgt acaaaatcag tgattaggaa taatcttagc cgccttaaca aacacataca
    11401 aaaacagcgg ttatgtttca tctcaccaca ataagtaacg acagtatcat gctaaaaaaa
    11461 gatgatatta tctttatgaa atcataacat gttgttttta ttaaattaat caggcattta
    11521 tggactggat ttcacttgct atcgccattg ctctacttgt tgtagctgtc tacacaggaa
    11581 tctcatacta caaagaaaaa aaaacaatca gtcgtgcatt taggcgatag cgccctattt
    11641 accataatcc cttcttacgc gcaccgcaga aacggcctca gggcgtttct gcgccttcag
    11701 ccacaaagag cacgtcttcc cccagaaaac cgcccggaat cgctctggtg agttttacgg
    11761 gggtgaatga ctatgcaccg tagtgaatac cacgctgttt cgccctgaac tccccacgaa
    11821 accgacgaaa gtggcaatca gcgacgcggc gcccgcggac gcgaccgggt aaattccgcc
    11881 tgctgccgct cctcctcacg cgcagatcgc tgccgttctg cctggcgctg cgattcgatc
    11941 tgtgcacgag ccctgttaag gacaatgtgc ctgttgggct gattcagctt cttgcctgac
    12001 gctctctcag cggcggcaac agcatcgtcg agaaatcttt tctcggcggg cgtcaggttt
    12061 atcagtcgtt tcatggcgat ctccggtcag aataactctg cacagtctgc cagacacggc
    12121 gaacctcatg aagaacctgc agccgggaac agccctgaga tatcgttttg gtgccggcat
    12181 cgtgatggcg gtactccaaa aaacacatct tgtcccgctt tcagtttctg aatttgcggg
    12241 ttccgggcgc agcgccggac cagtcacgta gcgctagcgg agtgtatact gccttagtat
    12301 gttggcatga gtgcgataat caggaaagcg ccttatgtgg caggagaaaa atgctgcagc
    12361 agagcgtaag cagaatatgc gatacagaag atattccgct tcctcgctca ctgactcgct
    12421 tcgctcggtc gttcggctgc ggcgagcggt acagacttac ggaagggcgg agatttcctg
    12481 gaagatgcca agaggatact taacagggaa gcgatagggc cgcggcgaag ccgtttttcc
    12541 ataggctccg cccccctgac aagcatcaca aaatctgacg ctcaaatcag tggcggcgaa
    12601 acccgacagg acttaaagat accaggcgtt tccccctggt cgctccctcg tgcgctctcc
    12661 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc
    12721 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
    12781 ccccccgttc agtccgactg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    12841 gtaagacatg caaaagcgcc actggcagca gccactggta actgatttag aggagatagt
    12901 cttgaagtca tgcgccagtt aaggctaaac taaacgaaca gttttggtga ctgcgctcct
    12961 ctaactagcc agttacctcg gttcaagagt tgctgctctg agaaccttcg agaaaaccgc
    13021 cttgctgggc ggtttttcgt tttcagagca agagattacg acgagactga atgaatctca
    13081 agatttcatt tagcctgcca cccgatcatt caaagttttg gcgtagttct gacccgctcc
    13141 ccgtgatttt tacataccca ccacgggggc aataaggttt ctattgcgtt ttttcgataa
    13201 aacccgtagg ttttagaggt gattagctga gaggttccgc tccacaagag agatatagcg
    13261 agatgattcg tccgctgaat aatcctaaca tctagtaact tagcttacgc taaaccatgg
    13321 cccacagggc aatgtatgga ctagaacgta atcacacgtc gagaacatta tgttctcgac
    13381 actaatttga tccagttata cagcccgata gcattgtcta tgttggcgtt atgtattgct
    13441 gatctaagtt ttctttttgt tttccttgtt ggctttatga tttctcttg
//
