LOCUS       NZ_CP138432             3264 bp    DNA     circular CON 20-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 64EVAM plasmid p64EVAM-1, complete
            sequence.
ACCESSION   NZ_CP138432
VERSION     NZ_CP138432.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38124869
            Assembly: GCF_033802585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Tet(X4) producing Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2023) Henning Seedorf Group, Temasek Life Sciences
            Laboratory Limited, 1 Research Link National University of Singapore
            Singapore, Singapore 117604, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9; Nanopolish v. 0.13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 499x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/20/2023 01:48:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,825
            CDSs (total)                      :: 5,697
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 93 of 187
            Pseudo Genes (incomplete)         :: 104 of 187
            Pseudo Genes (internal stop)      :: 24 of 187
            Pseudo Genes (multiple problems)  :: 30 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP138432.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="64EVAM"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p64EVAM-1"
                     /geo_loc_name="Singapore"
                     /collection_date="2019-01"
     gene            complement(534..1541)
                     /locus_tag="SE701_RS26580"
     CDS             complement(534..1541)
                     /locus_tag="SE701_RS26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131685.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_065808204.1"
                     /translation="MNIKSALLSAAMAAVSVNCFADTPAPDRHVQQFLDALNGGGGKPI
                     EQLSPQAARQVLIDAQKGATLPAADVSEKTISVDGLNITLKIVKPHNAQGTLPVFMFFH
                     GGGWILGDFQTHERLIRDLVSQSGAAAVYVNYTPSPEAHFPVAINQAYAATRWVAENGS
                     EIGVDGKRLALVGNSVGGNMVAAVALQAKQHKSPAIRYNVMLWPVTDANFDTGSYNQYQ
                     NGYFLSRNMMKWFWDAYTTKESERNNILASPLRATREQLKGLPPTLIQTAEFDVLRDEG
                     EAFGRKLDAAGVDVTVTRYNGLIHDFGLLNALSDVPAVRAAIGQAAGQLKEHLK"
                     /name="alpha/beta hydrolase"
     gene            complement(1758..2523)
                     /locus_tag="SE701_RS26585"
                     /pseudo=""
     CDS             complement(1758..2523)
                     /locus_tag="SE701_RS26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050724.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /name="alpha/beta hydrolase"
     gene            2676..3143
                     /locus_tag="SE701_RS26590"
     CDS             2676..3143
                     /locus_tag="SE701_RS26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884107.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_217259627.1"
                     /translation="MTKDINTGDLLPDVHGMLCFSLYSAGHAFTQLYRPMLDKLGLTYP
                     QYLVMITLWNQDGRTVKELSGILFLESSTLTPLLKRLETAGLITRTRNPKDEREVLIHL
                     TEKGNRLKIEASNIMRCIVDTLEMDADVVADIKASVDKIRDQIHRAHKPDI"
                     /name="MarR family transcriptional regulator"
ORIGIN
        1 atcatcttga gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattggta
       61 agagtcgatg tgcgagagat ttagagagta cttgaagtgg tggcctgagt gcggctacac
      121 tgaaaggaca gttttggtga ctcgtctcgc acaagacagt tacctggtta agcagttccc
      181 caactgactt gaccttcgat caaaccacct ccccaggtgg tttttttcgt tttcagagca
      241 agagattacg cgcagaggaa aaagatctca aggagatcct tttcagatcg atgaatacac
      301 cattatttca tcgacagtag cggtaatgac tccaacttac tgatagtgtt ttatgttcag
      361 atacgaacaa ggggaacccc ccccccgggg atgcggacac tcccatacct ctcgggggga
      421 aactaaaaaa aactgaaggc gctgtcccgg caaatccgga acctaatcag gatgacaagc
      481 agatggccga gggttcagcg accagccggc ggtaccggct ggtcacttgt tcgttatttc
      541 agatgttctt tcagctggcc ggcggcctgg cctatggctg cccgtacggc aggaacgtca
      601 ctcagcgcat tcagcaaacc aaaatcatgg atcaagccgt tataccgggt caccgtcaca
      661 tccactcccg ctgcgtcgag cttacgtccg aacgcttctc cctcatcacg aagcacgtca
      721 aactcggcag tctggatcag tgtgggtggt aagcccttca gctgctcacg cgttgcacgc
      781 agtggtgagg cgagaatatt attacgctcg ctctctttgg tcgtataggc atcccagaac
      841 cacttcatca tgttccgtga caggaaataa ccgttttggt actgattata agaaccggta
      901 tcaaaatttg cgtcagtgac aggccataac atgacattgt agcgaatggc aggtgactta
      961 tgctgttttg cctgaagcgc tacggcggca accatgttac ctcccacgct gttaccgaca
     1021 agggctaacc gtttgccatc cacgccaatc tcgctgccgt tttcagccac ccagcgggta
     1081 gcagcatatg cctgattgat agcaaccggg aagtgcgctt ctggtgacgg ggtgtagttg
     1141 acatagaccg ctgctgctcc ggactgactg accaggtcac gaataagacg ctcatgtgtc
     1201 tggaaatcgc ccagtatcca gccaccaccg tggaagaaca tgaaaacagg cagagtgcct
     1261 tgtgcgttgt gaggcttcac aattttcagc gtaatattca ggccatcaac actgatcgtt
     1321 ttttcgctga cgtccgctgc cggtagcgtg gcgcctttct gcgcatcaat caacacctga
     1381 cgtgcggcct ggggagataa ttgttcgatg ggctttcccc cgcctccgtt taatgcatcc
     1441 aggaattgct ggacatggcg atccggtgca ggtgtatcag caaaacagtt gactgaaaca
     1501 gctgccatcg cggcagacag taatgccgat ttgatattca ttgtgttacc tcattaacgt
     1561 tgcttgcaat taaattgcaa actactcata tgattaagaa ataatctgta tcaacacttt
     1621 aataaacatt cctgtcatct cacatgtgaa tgccatctca ccagtaaggg gtattcacat
     1681 caccgaagca gagcaggggc gtaagggaaa agacagggcc cctgcgggaa atggagttaa
     1741 agacaggaca aagacgttta ttttgctgct tcctcaataa gtgtcgctac gtcctgagga
     1801 tgagaaacca tcacgacatg ggatgcccct ttgatcacaa cggtgtgttt tgatcccgcg
     1861 cgttcagcca tgaaacgcaa acttttgaca gggatatttt tgtcgctgtc accgtaaatg
     1921 aagtaagaag gcagtgtttt ccaggcggga gcccctgatt ttcagttaat gctgcttcgg
     1981 tcacaggtct ttgtcctgcc gccattaatg cagcattttc agctgacacg tcatgagcga
     2041 attgctgatg gaacttcgac tgctcaatgg acagatcgac gtttccatca gccagcttaa
     2101 ccggtgcggc aagcgcttcg ccaagcgtgc ttccggggaa tttccccgcc agttcagcgg
     2161 ccgcttcacc cgcgtcagga gcaaatgcgg cgacataaac cagtgactta acattttttg
     2221 tttcccgggc tgcattcgtt attacctgac caccatagga atgcccgaca agtacaacgg
     2281 gtcccttaat attgttcaca acagaagaga tgtaagccgc atcagttgaa acactgcgca
     2341 atggattagc cgctgcgacg acgttatacc cgtcttttgc aggatccggg tgacaccatt
     2401 ccagctagaa gagtcagcaa aggcaccatg gaccaggaca acagtgggct ttgcggtttc
     2461 ggcgacagca ctggcagaca gcagagcggt ggcaatggcg agggaggcag caaatttaga
     2521 cataatagtt tctctcttca acggttgtat gttcgtgtgc aatttgtttg tgcgcgattt
     2581 gtttgtatgc aaattaagat agtactcctt tttgtcgttg tcaatcgctt gcaatcaaat
     2641 cgcacacaaa ttattttaga ggcactggag gacatatgac taaagacatc aataccggag
     2701 atttactccc ggatgtgcac ggcatgctct gcttctctct ctactccgcg ggacatgctt
     2761 ttacgcagct ctatcgtcca atgctcgata agctgggatt aacctatccc cagtacctgg
     2821 tgatgatcac gttgtggaat caggatggac gtactgtgaa ggaactgagt ggaatactct
     2881 tccttgagtc cagcacgctg actcctctac tcaagcggct ggagacggcg ggcttgatca
     2941 cccggacacg caatccgaag gatgaacgtg aagtgcttat tcatctcaca gagaagggga
     3001 acagattgaa aattgaggcc agcaatatca tgcgctgtat tgtggatacc cttgagatgg
     3061 atgcggatgt ggttgctgac atcaaagcct cagtcgataa gatccgtgat cagatacatc
     3121 gtgcacataa accggatatc tgatgatgct ataaagcaat atggccctac ataacattaa
     3181 gttatagggg cgtatggagc tgcatgacaa ggtcatcggg cattatctga acataaaaca
     3241 ctatcagtaa gttggagtca ttac
//
